• 한국동물학회:학술대회논문집
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• 한국동물학회 1995년도 한국생물과학협회 학술발표대회
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• pp.318.2-318
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• 1995

• Bulletin of the Korean Chemical Society
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• 제20권6호
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• pp.753-756
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• 1999

• 한국작물학회지
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• 제52권1호
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• pp.100-103
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• 2007

A field experiment was conducted in Agronomy farm at an altitude of 1350 m. above sea level in a randomized complete block design with three replications and eight treatments on vegetable type soybean in 2005. The objective was to see the effect of vermicompost alone and in combination with Rhizobium inoculum and mineral fertilizers on the yield of vegetable soybean. The result showed higher number of nodules from the non inoculated plot, however, the nodules weight was highest from the inoculated plots. No significant difference was notice statistically on roots and shoots dry weight. Soybean grain was obtained highest (32.3%) over the non inoculated plot followed by vermicompost plus inoculated, however, there was non significant difference. There was a slight increment on grain and straw yield from the inoculated plots over the non inoculated.

• BMB Reports
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• 제35권5호
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• pp.443-451
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• 2002

Malonate is a three-carbon dicarboxylic acid. It is well known as a competitive inhibitor of succinate dehydrogenase. It occurs naturally in biological systems, such as legumes and developing rat brains, which indicates that it may play an important role in symbiotic nitrogen metabolism and brain development. Recently, enzymes that are related to malonate metabolism were discovered and characterized. The genes that encode the enzymes were isolated, and the regulation of their expression was also studied. The mutant bacteria, in which the malonate-metabolizing gene was deleted, lost its primary function, symbiosis, between Rhizobium leguminosarium bv trifolii and clover. This suggests that malonate metabolism is essential in symbiotic nitrogen metabolism, at least in clover nodules. In addition to these, the genes matB and matC have been successfully used for generation of the industrial strain of Streptomyces for the production of antibiotics.

• Journal of Microbiology and Biotechnology
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• 제16권5호
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• pp.791-794
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• 2006

Cyclosophoraose (cyclic $\beta-(1,2)-glucan$, Cys) isolated from Rhizobium meliloti, a soil microorganism, was used as a solubility enhancer for flavonoids. The complexes of the cyclic oligosaccharide with flavonoids were confirmed through $^1H$ nuclear magnetic resonance (NMR) spectroscopic analysis. Flavonoids solubilized by Cys were quantitatively analyzed through high-performance liquid chromatography (HPLC). Among the flavonoids tested, the solubility of naringenin was greatly enhanced by Cys, compared with other compounds. The solubility of naringenin was enhanced about 7.1-fold by adding 10 mM Cys, compared with a control. $^1H$ NMR spectroscopic analysis indicated that the H-6 and H-8 protons, which are located on the A ring of naringenin, were greatly shifted upfield upon the complexation with Cys. This result suggested that Cys showed a regioselective interaction with the naringenin molecule upon the complexation, resulting in the solubility enhancement of naringenin.

• Applied Biological Chemistry
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• 제30권2호
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• pp.153-162
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• 1987

한국에서 시험재배 되고 있는 대두(Glycine max.) 101품종의 근류로 부터 근류균을 분리하여 AMA 고체배지 상에서의 생육속도 및 근류형성력에 따라 fast-growing soybean rhizobia 7 균주와 slow-growing soybean rhizobia 9균주를 선발하였다. 선정균의 질소고정력, 미생물학적 특징 및 생화학적 특징을 조사한 결과 slow-growing soybean rhizobia 는 .Bradyrhizobium japonicum 과 일치하였으며, fast-growing soybean rhizobia는 Rhizobium fredii 와 유사하였다.

• Bulletin of the Korean Chemical Society
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• 제27권9호
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• pp.1485-1488
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• 2006

• BMB Reports
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• 제32권4호
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• pp.414-418
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• 1999

A novel gene for malonyl-CoA decarboxylase was discovered in the mat operon, which encodes a set of genes involved in the malonate metabolism of Rhizobium trifolii (An and Kim, 1998). The subunit mass determined by SDS-PAGE was 53 kDa, which correspond to the deduced mass from the sequence data. The molecular mass of the native enzyme determined by field flow fractionation was 208 kDa, indicating that R. trifolii malonyl-CoA decarboxylase is homotetrameric. R. trifolii malonyl-CoA decarboxylase converted malonyl-CoA to acetyl-CoA with a specific activity of 100 unit/mg protein. Methylmalonyl-CoA was decarboxylated with a specific activity of 0.1 unit/mg protein. p-Chloromercuribenzoate inhibited this enzyme activity, suggesting that thiol group(s) is(are) essential for this enzyme catalysis. Database analysis showed that malonyl-CoA decarboxylase from R. trifolii shared 32.7% and 28.1% identity in amino acid sequence with those from goose and human, respectively, and it would be located in the cytoplasm. However, there is no sequence homology between this enzyme and that from Saccharopolyspora erythreus, suggesting that malonyl-CoA decarboxylases from human, goose, and R. trifolii are in the same class, whereas that from S. erythreus is in a different class or even a different enzyme, methylmalonyl-CoA decarboxylase. According to the homology analysis, Cys-214 among three cysteine residues in the enzyme was found in the homologous region, suggesting that the cysteine was located at or near the active site and plays a critical role in catalysis.

• 한국토양비료학회지
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• 제16권2호
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• pp.188-194
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• 1983

토양비옥도가 극(極)히 낮은 신개간지(新開墾地)에 있어서 근류균(根瘤菌)의 접종(接種) 및 비료(肥料)와 개량제 시용(施用)이 질소고정(窒素固定)과 대두수량에 미치는 영향(影響)을 밝히고자 pot시험(試驗)을 수행하였던 바 그 결과(結果)를 요약(要約)하면 다음과 같다. 1. 대두(大豆) 근류(根瘤)에 의(依)한 질소고정량(窒素固定量)은 근류균(根瘤菌)의 접종(接種)과 비료(肥料) 및 개량제 시용(施用)으로 현저히 증가하였다. 2. 근류균(根瘤菌) 접종(接種)으로 질소흡수량 및 질소고정량(窒素固定量)을 높여 콩수량을 4~10% 증대시켰다. 3. 콩 다수확재배기술체계 확립(確立)을 위하여 토양비옥도가 극(極)히 낮은 신개간지(新開墾地)에서는 개량제의 시용(施用)과 근류균(根瘤菌)과 접종(接種)이 병행되어야 할 것으로 나타났다.

• 한국미생물·생명공학회지
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• 제13권1호
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• pp.13-17
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• 1985

국내에서 분리된 대두근류균을 생육속도에 따라서 2.4시간의 평균세대시간을 가지는 fast-grower와 13.1시간의 평균세대시간을 가지는 slow-grower로 나눌 수 있었다. Fest-grower는 6-phosogluconate dehydrogenase 효소역가가 slow-grower 보다 높았으며 sucrose를 탄소원으로 이용하였다. Fest-grower 중에서 특히 R4, R278은 NaCl 0.5M농 도에서도 생육하였으며 시험한 대두 근류균은 모두 pH 4.5 이하에서 생육이 저지되었다. 또한 식물배양기 조건하에서 접종실험을 한 결과 시험된 모든 대두품종에 양호하게 근류를 형성하였으며 L-259 (NRRL) 균주에 비해서 0.1-2배의 질소고정력을 나타내었다.