• 대한화장품학회지
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• 제22권1호
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• pp.40-59
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• 1996

Retinol은 열과 빛에 쉽게 산화되기 때문에 그 유도체인 Retinyl Palmitate를 사용하고 있으나 이것 또한 안정성이 우려되어 많은 양을 사용하지 못하고 있다. 본 연구에서는 Retinyl Palmitate 5% 와 Tocopgeryl Acetate 10%를 함유한 W/O, W/S, O/W, MLV Type의 크림에서 열적 안정성 및 UV 안정성을 CHROMA METERS로 측정하였으며, $25^{\circ}C$, $45^{\circ}C$에서 RP의 함량을 HPLC로 정량 분석하였다. 또한 RP가 열, 빛 등에 의해서 어떤 물질로 변화하였는지를 측정하였으며, 변화된 물질의 독성에 대한 연구를 하였다. 각각의 크림 TYPE 마다 입자 SIZE를 측정했으며, DNA와 CHROMA METERS를 사용하여 CELLULAR RENEWAL을 측정하였다.

• 대한수의학회지
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• 제44권2호
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• pp.185-193
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• 2004

The effects of membrane lipid peroxidation and retinyl palmitate on rat liver microsomal functions were investigated in vitro. Rat liver homogenates exposed to oxygen tension for 0, 3, 6, 9 or12 hours and lipid peroxidation levels were evaluated by the measurements of fluorescence intensity, malondialdehyde (MDA) and retinyl palmitate. The fluorescence intensity of homogenates and microsomes were elevated and retinyl palmitate concentrations were decreased. But the concentration of MDA was not affected to exposure time. Therefore, fluorescence intensity and retinyl palmitate concentration were used to analyze the correlation between lipid peroxidation and microsomal functions. To investigate the liver microsomal functions, the microsome was isolated from rat liver homogenates exposed to oxygen. The concentration of cytochrome P450 and the activity of NADPH-cytochrome P450 reductase in liver microsomes were gradually decreased with increasing the exposure time. The correlation between fluorescence intensity of microsomes showed a very high inverse correlation of -0.97 and -0.93, respectively. The decrease of cytochrome P450 concentration was due to the regeneration of cytochrome P450 to cytochrome P420. Also, the activities of cytochrome P450-dependent aminopyrine demethylase and benzpyrene hydroxylase of liver microsomes were gradually decreased with increasing the exposure time. The correlation with fluorescence intensity of microsome showed a high inverse correlation of -0.97 and -0.91, respectively. The retinyl palmitate concentrations of rat liver homogenates were decreased with increasing the exposure time. The decrease of retinyl palmitate concentration was followed by a low concentration of cytochrome P450 and activity of NADPH-cytochrome P450 reductase. The correlation indicated high direct correlation of 0.92 and 0.93, respectively. The decrease of retinyl palmitate concentration was also accompanied by the reduction of aminopyrine demethylase and benzpyrene hydroxylase activities. The correlation was analyzed a high direct correlation of 0.90 and 0.85, respectively. In conclusion, these studies have shown that the membrane lipid peroxidation of rat liver microsome proportionally decreased microsomal enzyme activities in vitro experiments.

• 한국임상수의학회지
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• 제19권1호
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• pp.43-48
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• 2002

This study was performed to investigate the effects of recombinant BST formulation treatment on the milk yield, milk components, mastitis, and general cow health condition when the formulations of retinyl palmitate, cholecalciferol and rBST were administered after the peak period of milk production. The milk yields of treatment groups (Group I, II, III and IV) were increased from 21.5% to 29.0% than that of control group. There was significant difference in milk production between treatment group II, IV and control group (P<0.05). However, there was no significant difference in milk production between treatment group I, III and control group (P<0.05). And the addition of retinyl palmitate and cholecalciferol into rBST formulation did not increase the milk yield. The milk of treatment groups with sustained-release rBST did not show significant difference in milk components (milk fat, protein, lactose, and solid not fat). However, there were minor changes, primarily in fat content of milk, during the first few weeks of rBST administration. There was no incidence of clinical mastitis between rBST treatment groups and control group. Addition of high and medium concentration of retinyl palmitate and cholecalciferol into sustained-release rBST formulation was efficient in reduction of somatic cell count in milk. There was great energy deficit in all treatment groups compared with control group during the early study period. Thus, the body condition score of all treatment groups showed lower value than control group. No evidences of metabolic health problems, such as ketosis, milk fever, and downer cow were observed. Incidence of general lameness did not appear on all treatment groups during 140 days of this study.

• Asian-Australasian Journal of Animal Sciences
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• 제33권12호
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• pp.1985-1990
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• 2020

Objective: The objective of this experiment was to investigate the effects of fat-soluble vitamin injection on plasma and tissue vitamin status in nursery pigs. Methods: A total of 16 pigs (initial body weight: 7.15±1.1 kg) were allotted to 2 treatments at d 7 post-weaning. Pigs were fed a corn-soybean meal-based basal diet with no supplemental vitamin A and i.m. injected with 300,000 IU of retinyl palmitate, 900 IU of d-α-tocopherol and 30,000 IU of vitamin D₃ with control pigs having no vitamin injection. Blood (d 0, 3, 7, and 14 post-injection) and tissue samples (liver, brain, heart, lung, and muscle; d 7 and 14 post-injection) were collected from pigs. Retinyl palmitate, retinol, and α-tocopherol concentrations were analyzed in plasma and tissues, while plasma was assayed for 25-hydroxycholecalciferol (25-OHD₃). Results: Plasma retinol and 25-OHD₃ concentrations increased by the vitamin injection from d 3 to 14 post-injection (p<0.05) whereas plasma retinyl palmitate was detected only in the vitamin treatment at d 3 and 7 post-injection (115.51 and 4.97 ㎍/mL, respectively). Liver retinol, retinyl palmitate, and retinol+retinyl palmitate concentrations increased by retinyl palmitate injection at d 7 and 14 post-injection (p<0.05) whereas those were not detected in the other tissues. The d-α-tocopherol injection increased α-tocopherol concentrations in plasma at d 3 and 7 post-injection (p<0.05) and in liver, heart (p<0.10), and muscle (p<0.05) at d 7 post-injection. Conclusion: Fat-soluble vitamin injection increased plasma status of α-tocopherol, retinol, retinyl palmitate and 25-OHD₃. As plasma levels decreased post-injection, vitamin A level in liver and vitamin E level in muscle, heart and liver increased. The α-tocopherol found in plasma after injection was distributed to various tissues but retinyl palmitate only to the liver.

• 대한화장품학회지
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• 제34권3호
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• pp.175-181
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• 2008

생체 친화성 에칠셀룰로오스 막제를 사용하여 자발적인 유화확산에 의하여 레티닐 팔미테이트를 봉입한 나노입자를 제조하였다. 분산매인 유화제로는 폴리소로베이트 20, 폴리소르베이트 60, 피피지 26-부테스-26/피이지-40 하이드로제네이티드캐스터오일를 이용하여 제조하였으며, 본 결과로서 나노크기 입자를 제조하기에 가장 적절하며 안정한 조건은 1w/v% 에칠셀룰로오스와 3w/v% 폴리소그베이트 60 수용액을 이용한 것이었으며, 레티닐 팔미테이트의 봉입률을 최대화하기 위한 조건은 2w/v% 에칠셀룰로오스와 1w/v% 폴리소르베이트 60 수용액을 이용한 제조 조건이었다. 또한 이 최적 조건에 다른 유효성분에도 적용 가능한 것을 확인하였다.

• Journal of Applied Biological Chemistry
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• 제65권4호
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• pp.307-312
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• 2022

A hair essence containing α-tocopheryl acetate, retinyl palmitate, and phytantriol (TRP-hair essence) was prepared. TRP-hair essence was excellent in thermal stability to the extent that it did not harden at all even at 210 ℃. TRP-hair essence potently protected the hair from heat stress, significantly reducing the protein leakage in heat-treated hair (p <0.001). Treatment of TRP-hair essence to dyed human hair significantly protected hair against heat stress (p <0.05) as well as improved hair cuticle and color persistence (p <0.05). In addition, as a result of directly treating human hair with TRP-hair essence, the cuticle and tensile strength of human hair were significantly improved (p <0.05). These results suggest that TRP-hair essence can be effective for hair protection and hair quality improvement.

• 대한화장품학회지
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• 제23권3호
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• pp.48-74
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• 1997

Retinlyl Palmitate, the skin normalizerm is useful to promote greater skin elasticity, to diminish lipid peroxidation and skin roughness following UV exposure, and promote a youthfulgeneral skin appearance. In manufacturing creams, Retinyl Palmitate, which is a derivative of retinol, is used since reionol is easily oxidized by heat and light. However, only a small mount of retinol, is used since using a large amount of it may be harmful to its stability. In this study, thermal stability and UV stability of W/O-, W/S-, O/W-, and MLV-type creams containing 5% of retinyl palmitate and 10% of tocopheryl acetate are measured by Chroma Meters, and the content of RP is quantitatively analyzed by HPLC at 25 $^{\circ}C$ and 45 $^{\circ}C$. Also, how RP has been changed by heat, light, etc. is measured by HPLC, and toxicity of the changed substance is studied. Particle size of each type of the cream if measured, cellular renewal is measured by using DHP and Chroma Meters in order to study their efficacy and effect, moisture content is measured by using Corneometer and Tewameter, and how much wrinkles are improved is studied by using Image Analyzer. Development of MLV-type cream containing 5% of RP and 10% of TA, and satisfying conditions for better creams has been successful.

• Biomolecules & Therapeutics
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• 제22권1호
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• pp.73-77
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• 2014

The purpose of this study was to examine the effect of stabilization of retinyl palmitate (RP) on its skin permeation and distribution profiles. Skin permeation and distribution study were performed using Franz diffusion cells along with rat dorsal skin, and the effect of drug concentration and the addition of pectin on skin deposition profiles of RP was observed. The skin distribution of RP increased in a concentration dependent manner and the formulations containing 0.5 and 1 mg of pectin demonstrated significantly increased RP distributions in the epidermis. Furthermore, it was found that skin distribution of RP could be further improved by combined use of pectin and ascorbyl palmitate (AP), due largely to their anti-oxidative effect. These results clearly demonstrate that the skin deposition properties of RP can be improved by stabilizing RP with pectin. Therefore, it is strongly suggested that pectin could be used in the pharmaceutical and cosmetic formulations as an efficient stabilizing agent and as skin penetration modulator.

• Journal of Pharmaceutical Investigation
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• 제29권4호
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• pp.279-285
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• 1999

Retinoids in anti-wrinkle cosmetics are widely used for the treatment of the photoaging skin these days, but very unstable in the presence of water. Multilamellar liquid crystal vesicles consisted of $poly(oxyethylene)_{15}oleyl$ ether $(POE_{15}OE)$ or $poly(oxyethylene)_{15}stearyl$ ether $(POE_{15}SE)$, cetostearyl alcohol (CSA) and cholesterol or oils were prepared to increase the stability of retinyl acetate or retinyl palmitate. $POE_{15}SE-CSA-Water$ systems were more unstable than $POE_{15}SE-CSA-Water$ systems due to the bended structure of oleyl groups. For the modification of vesicles, cholesterol was added. The enthalpy values were decreased reaching to zero and the water permeability of systems was decreased, but the stabilizaton of retinyl acetate was not greatly improved. The stability of retinyl acetate may depend on its location in the structure. Various oils were added for further stabilization and isopropyl myristate was most effective. In practice, retinyl palmitate showed better stability in the same vesicle than retinyl acetate, and the improved vesicles could be used for the anti-wrinkle cosmetics

• 대한화장품학회:학술대회논문집
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• 대한화장품학회 1999년도 IFSCC . ASCS 학술대회 발표 논문
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• pp.145-154
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• 1999

A novel retinol derivative, polyethoxylated retinamide (Medimin A) was synthesized, as an anti-aging agent. Collagen synthesis, skin permeation, stability, and toxicity of Medimin A were evaluated and compared with those of retinol and retinyl palmitate. In vitro collagen synthesis was evaluated by quantitative assay of [$^3H$]-proline incorporation into collagenase sensitive protein in fibroblast cultures. For in vitro skin permeation experiments, Franz diffusion cells (effective diffusion area: $1, 766{\;}\textrm{cm}^2$) and the excised skin of female hairless mouse aged 8 weeks were used The stabilities of retlnoids were evaluated at two different temperature ($25{\;}^{\circ}C$ and $40{\;}^{\circ}C$) and under UV in solubilized state and in OW emulsion. To estimate the safety, acute oral toxicity, acute dermal toxicity, primary skin irritation, acute eye irritation and human patch test were performed The effect of Medimin A on collagen synthesis was similar to that of retinol. The skin permeability of Medimin A was higher than those of retinol and retinyl palmitate. The Medimin A was more stable than retinol and retinyl palmitate. Medimin A was nontoxic in various toxicological tests. These results suggest that Medimin A would be a good anti-aging agent for enhancing bioavailability and stability.