• Title/Summary/Keyword: restriction map.

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Genetic map of MCPA plasmid isolated from Pseudomonas sp. I

  • Park, Young-Doo
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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    • 2010.10a
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    • pp.663-665
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    • 2010
  • By the curing and transformation experiment, it was found thatthe genes of Pseudomonas sp.KU171(pKU19) for MCPA-degrading were located on a plasmid pKU19. Also the plasmid had degradative gens for 2,4-D, 3CB, and DCP. Molecular size of pKU19 was measured to be 31.2Kb. The restriction pattern were analyzed with Eco RI, BglII,XhoI, and the restriction map was generated.

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Drosophila robusta species group 2종 (D.lacertosa 와 D.sordidula)의 mtDNA 변이에 의한 종분화정도

  • 최준길;박제철
    • Animal Systematics, Evolution and Diversity
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    • v.11 no.4
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    • pp.469-477
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    • 1995
  • Drosophila lacertosa and D. sordidula are members of the robusta species group in virilis section of Drosophila. The mtDNA of both species was analyzed, using 10 restriction endonucleases. The mtDNA genome size of D. lacertosa and D. sordidula was 15.7 kbp, altogether, and the numbers of mtDNA fragment were 26 and 32, respectively. Restriction cleavage map of mtDNA in these species was constructed. The patterns of cleavage map were very different between two species and it means that speciation was taken for a long time ago.

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Genetic Analysis of Mitochondrial DNA from Korean Oysters, Crassostrea gigas (한국산 참굴(Crassostrea gigas) 미토콘드리아 DNA의 유전적 분석)

  • KIM Sang Hae;PARK Mi Seon;KIM Young Hun;PARK Doo Won
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.30 no.5
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    • pp.804-808
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    • 1997
  • The genetic differentiation and characteristics of two oyster populations (Crassostrea gigas) in Korea were assessed based on the restriction fragment length polymorphisms (RFLP) analysis and the restriction patterns of subcloned mtDNA. The restriction fragments of twenty individuals in West Sea revealed an identical pattern, determined by 8 restriction enzymes. On the other hand, two haplotypes having variation at the HindIII site were shown in the specimens from South Sea; minor haplotypes (4 of 20) were similar to the results obtained from individuals in West Sea while major haplotypes were different from those in West Sea. It was suggested that oysters (C. gigas) of West Sea might have been introduced to South Sea. Each mitochondrial DNA from two oyster populations in Korea and from one in Japan was divided to three parts and subcloned into pUC19 to use in genetic studies effectively. Restriction map was constructed based on the cleavage pattern by multiple restriction enzymes.

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Characterization of Multidrug Resistant Plasmid of Staphylococcus aureus (황색포도상구균의 항생제 다제내성을 갖는 플라스미드의 동정)

  • Kim, Ki-Hyun;Lee, Dae-Woon;Kim, Jong-Myung;Moon, Kyung-Ho
    • YAKHAK HOEJI
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    • v.36 no.5
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    • pp.486-490
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    • 1992
  • The clinical isolate Staphylococcus aureus SA2 was resistant to ampicillin, Chloramphenicol, clindamycin, erythromycin, gentamicin, kanamycin, methicillin, streptomycin, and tobramycin and harboured more than two kinds of plasmids. Transformation experiment demonstrated that 40.98-kb plasmid(pKH2) encoded resistance to ampicillin, clindamycin, erythromycin, kanamycin, and streptomycin. The cleavage map of a pKH2 was determined by restriction enzyme mapping techniques. Cleavage map is given for BamHI, BglI, BstEII, SalI and XhoI.

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Mobile Vector Map Generalization Methods for Location Information Search

  • Choi, Jin-Oh
    • Journal of information and communication convergence engineering
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    • v.6 no.2
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    • pp.187-191
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    • 2008
  • In the mobile environments for the vector map services, a map simplification work through the map generalization steps helps improve the readability of the map on a large scale. The generalization operations are various such as selection, aggregation, simplification, displacement, and so on, the formal operation algorithms have not been built yet. Because the algorithms require deep special knowledge and heuristic, which make it hard to automate the processes. This thesis proposes some map generalization algorithms specialized in mobile vector map services, based on previous works. This thesis will show the detail to adapt the approaches on the mobile environment, to display complex spatial objects efficiently on the mobile devices which have restriction on the resources.

Molecular Cloning and Expression of Bacillus pasteurii Urease Gene in Escherichia coli (B. pasteurii Urease 유전인자의 E. coli의 복제와 발현)

  • Kim, Sang-Dal;John Spizizen
    • Microbiology and Biotechnology Letters
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    • v.13 no.3
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    • pp.297-302
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    • 1985
  • The 7.1 Mdal Xbaf fragment of Bacillus pasteurii ATCC 11859 containing gene for urease was inserted into the Xbal site of bifunctional plasmid pGR71, and its urease gene was cloned and expressed in E. coil RRI. But the cloned gene was not expressed in Bacillus subtilis BR151 in consequence of deletion of inserted DNA fragment. The recombinant plasmid thus formed was named pGU66. The restriction map of the plasmid pGU66 was determined, and the size of the plasmid was estimated to be 12.6 Mdal by double digestion of restriction enzymes of the plasmid. The urease of the cloned strain was accumulated in periplasmic space and very similiar to that of donor strains in their enzymatic properties.

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DIGITAL (k0,k1)-COVERING MAP AND ITS PROPERTIES

  • HAN, SANG-EON
    • Honam Mathematical Journal
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    • v.26 no.1
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    • pp.107-117
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    • 2004
  • The aim of this paper is to introduce a digital $({\kappa}_0,\;{\kappa}_1)$-covering map and a local $({\kappa}_0,\;{\kappa}_1)$-homeomorphism. And further, we show that a digital $({\kappa}_0,\;{\kappa}_1)$-covering map is a local $({\kappa}_0,\;{\kappa}_1)$-homeomorphism and the converse does not hold. Finally, some property of a digital covering map is investigated with relation to some restriction map. Furthermore, we raise an open problem with relation to the product covering map.

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Study on the Filtering Methods for Mobile Vector Map Service (모바일 벡터 맵 서비스를 위한 필터링 기법 연구)

  • Choi Jin-Ho;Lee Sang-Wook
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.10 no.9
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    • pp.1612-1616
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    • 2006
  • For map services in the mobile environment, it should be considered that resource restriction or the mobile device. on, if a map database dedicated to mobile services may not be developed, the spatial data extracted from general map databases should be simplified before transmitting. % is paper suggests the filtering methods to manipulate the spatial data, which are changed to be able to displayed on the mobile devices. The suggested methods are evaluated by experiments. This method is based on the map generalization operator 'selection' and is refined to adapt on mobile phone environments.

SEQUENCE ANALYSIS AND COMPARISON OF BOVINE αS1-CASEIN GENOMIC DNA

  • Lin, C.S.;Huang, M.C.;Choo, K.B.;Tseng, Y.H.
    • Asian-Australasian Journal of Animal Sciences
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    • v.6 no.4
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    • pp.541-547
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    • 1993
  • A phage clone containing the partial ${\alpha}_{S1}$-casein gene was isolated from a bovine genomic library by using mixed probes of ovine ${\alpha}_{S1}$-, ${\beta}$- and ${\kappa}$-casein cDNAs. Restriction enzyme mapping analysis for 14.6 kb revealed that the map was in conflict with the report of Meade et al. (1990), especially in the 3'-end fragment. Sequence analysis of 12.6 kb revealed a high AT/GC ratio (1.64); we have identified eight exon sequences according to the bovine ${\alpha}_{S1}$-casein cDNA sequence. The same exon/intron splice junction sequence was observed between these exons. We suggest that the bovine ${\alpha}_{S1}$-casein gene night contain a minimum of 18 exons and the full length is approximately 18-19 kb.