• Development and Reproduction
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• v.17 no.4
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• pp.379-387
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• 2013

Embryonic stem (ES) cells can self-renew and differentiate to various cells depending on the culture condition. Although ES cells are a good model for cell type specification and can be useful for application in clinics in the future, studies on ES cells have many experimental restraints including low transfection efficiency and transgene expression. Here, we observed that transgene expression after transfection was enhanced by treatment with histone deacetylse (HDAC) inhibitors such as trichostatin A, sodium butyrate, and valproic acid. Transfection was performed using conventional transfection reagents with a retroviral vector encoding GFP under the control of CMV promoter as a reporter. Treatment of ES cells with HDAC inhibitors after transfection increased population of GFP positive cells up to 180% compared with untreated control. ES cells showed normal expression of stem cell markers after treatment with HDAC inhibitors. Transgene expression was further enhanced by modifying transfection procedure. GFP positive cells selected after transfection were proved to have the stem cell properties. Our improved protocol for enhanced gene delivery and expression in mouse ES cells without hampering ES cell properties will be useful for study and application of ES cells.

• Steel and Composite Structures
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• v.26 no.2
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• pp.227-239
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• 2018

Integral abutment bridges (IABs) have no joint across the length of bridge and are therefore also known as jointless bridges. IABs have many advantages, such as structural integrity, efficiency, and stability. More importantly, IABs have proven to be have both low maintenance and construction costs. However, due to the restraints at both ends of the girder due to the absence of a gap (joint), special design considerations are required. For example, while replacing the deck slabs to extend the service life of the IAB, the buckling strength of the steel girder without a deck slab could be much smaller than the case with deck slab in place. With no deck slab, the addition of thermal expansion in the steel girders generates passive earth pressure from the abutment and if the applied axial force is greater than the buckling strength of the steel girders, buckling failure can occur. In this study, numerical simulations were performed to estimate the buckling strength of typical steel girders in IABs. The effects of girder length, the width of flange and thickness of flange, imperfection due to fabrication and construction errors on the buckling strengths of multiple and single girders in IABs are studied. The effect of girder spacing, span length ratio (for a three span girder) and self-weight effects on the buckling strength are also studied. For estimation of the reaction force of the abutment generated by the passive earth pressure of the soil, BA 42/96 (2003), PennDOT DM4 (2015) and the LTI proposed equations (2009) were used and the results obtained are compared with the buckling strength of the steel girders. Using the selected design equations and the results obtained from the numerical analysis, equations for preventing the buckling failure of steel girders during deck replacement for maintenance are presented.

• Journal of the Korea Concrete Institute
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• v.15 no.5
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• pp.759-767
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• 2003

This paper presents the simplified but more rational analysis method for the prediction of additional internal forces induced in integral abutment bridges. These internal forces depend upon the degree of restraint provided tc the deck by the backfill soil adjacent to the abutments and piles. In addition, effect of the relative flexural stiffness ratio among pile foundations, abutment, and superstructure on the structural behavior is also an important factor. The first part of the paper develops the stiffness matrices, written in terms of the soil stiffness, for the lateral and rotational restraints provided by the backfill soil adjacent to the abutment. The finite difference analysis is conducted and it is confirmed that the results are agreed well with the predictions obtained by the proposed method. The simplified spring model is used in the parametric study on the behavior of simple span and multi-span continuous integral abutment PSC beam bridges in which the abutment height and the flexural rigidity of piles are varied. These results are compared with those obtained by loading Rankine passive earth pressure according to the conventional method. From the results of parametric study, it was shown that the abutment height, the relative flexural rigidity of superstructure and piles, and the earth pressure induced by temperature change greatly affect the overall structural response of the bridge system. It may be possible to obtain more rational and economical designs for integral abutment bridges by the proposed method.

• Proceedings of the Computational Structural Engineering Institute Conference
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• 1991.10a
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• pp.111-114
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• 1991

The KMRR 4-wheel crane which has a span of 30.6m long shall be designed to maintain its structural integrity during and after seismic shocks. Horizontal and vertical FRS for OBE and SSE conditions at the crane support are after seismic shock. Horizontal and vertical FRS for analysis are 4% for OBE and 7% for SSE. The crane consists of girder, saddle main and auxiliary trolley, and necessaries. They are modeled as beam elements and lumped masses for the following 4 cases ; trolley at center of the crane with and without the rated load, trolley at end with and without the rated load. The static analysis as well as the linear dynamic analysis including frequency and response spectrum analysis are performed for the seismic qualification of the crane using the Finite Element Method. For the simplicity of the analysis, the decoupling criteria are considered for the crane rope and the crane supporting beams. The main sections of the crane are stiffened until the calculated stresses satisfy the allowable limits. The seismic resultant loads are used to design the seismic restraints of the saddle and the trolley to protect the clue from the seismic uplifting loads the study results have show that the seismic design of the KMRR crane is governed by the OBE condition. not by the SSE condition. This paper briefly describes the analysis procedure used in the seismic design of the KMRR crane, and summarizes the analysis results.

• Journal of the military operations research society of Korea
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• v.17 no.2
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• pp.1-15
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• 1991

If one were to say that war is political in its nature, then war could be one of the several ways of achieving political goals. Thus, the aspect of wars will, in certainty, be altered by the changes in political environment. Wars in the past broke out mainly due to ideological differences that ran to extremes and were of high intensity. However, wars today show such restraints as controlling them beforehand or during the war in anticipation of the post-war situation. The trend of ideas to actualize such wars in the effective operation of airpower is on the rise. Airpower normally possesses speed and operative flexibility as well as capability of destruction, so it is possible to destroy the politically declared targets clearly. Previous airpower was merely employed as means of helping to vertically detouring the spatial obstacle that the ground forces encountered. Over the years, due to the speedy improvement of aviation technology and of ideas concerning wars in space, more advanced application has been developed. but they also were nothing more than an auxiliary role to facilotate the ground forces, that needed a longer effective range of firepower and did not become forces with the right of self-determination, that is, the fact of decisive war that makes its outcome. However, under transitional strategic environment like that of the present, Airpower possesses not only the capability to operate as a decisive means of war but also as theories to support it. The advancement in air technology has enabled supremacy over targets in depth, and the development of electronic technology has empowered the improvement of degree of destruction but also triumphant war by means of an overwhelming supremacy in a relatively short period. Thus, the method of systematic destruction that accomplishes the stated goals while rejecting the damage of accumulative destruction has been realized. The progress of such a concept has also proved that the counter-force strategy that has been developed in nuclear strategy is useful in conventional warfare as well. Therefore, it can be said that the under today's strategic environment airpower is an outstanding means of military strategy that can deeply affect to achieve the national objectives.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2004.10a
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• pp.131-136
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• 2004

The limited slip differential(LSD) is a device which enables the driving force to be transmitted from one slipping wheel to another wheel in such case that the car is stuck in clay or snow. When the unwanted slipping occurs on one wheel, the LSD temporarily restraints the differential motion to transmit the driving force in the other wheel. So far, many types of LSD were developed such as mechanical lock type, disk clutch type, viscous coupling type, torsion type and multiple clutch type. However these types of LSD is too complicated and expensive, so it is used only for 4WD outdoor vehicles, military vehicles, and a portion of deluxe car. So, many studies has been devoted to improve new types of LSD to cover those demerits of existing LSDs that the hydraulic LSD is developed as arepresentative result of that. The hydraulic LSD which uses the principle of gear pump is packed with viscous oil in tight container. When a slip occurs on one wheel, the hydraulic LSD generates torque caused by high oil pressure in the container. This study has been devoted to suggest an improved hydraulic LSD. In order to achieve it, we designed a new type of hydraulic LSD, produced it and did a rig test with it on real vehicle. From the rig test, it has been confirmed that the new type of hydraulic LSD can be directly applied to exiting vehicles without changing the design criteria

• Journal of KIISE:Information Networking
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• v.30 no.2
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• pp.179-188
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• 2003

Advances in wireless networking technology has engendered a new paradigm of computing, called mobile computing, in which users carrying portable devices have access to a shared infrastructure independent of their physical locations. Wireless communication has some restraints such as disconnection, low bandwidth, a variation of available bandwidth, network heterogeneity, security risk, small storage, and low power. Power adaptation routing scheme overcome the shortage of power by adjusting the output power, was proposed. Existing power saving routing algorithm has some minor effect such as seceding from shortest path to minimize the power consumption, and number of nodes that Participate in routing than optimal because it select a next node with considering only consuming power. This paper supplements the weak point in the existing power saving routing algorithm as considering the gradual approach to final destination and the number of optimal nodes that participate in routing.

• Journal of the Korean Society for Advanced Composite Structures
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• v.4 no.2
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• pp.39-44
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• 2013

This study is aimed to examine the influence of the rotational stiffness of U-shaped ribs on the local buckling behaviors of laminated composite plates. Applying the orthotropic plates with eight layers of the layup $[(0^{\circ})4]s$ and $[(0^{\circ}/90^{\circ})2]s$, 3-dimensional finite element models for the U-rib stiffened plates were setup by using ABAQUS and then a series of eigenvalue analyses were conducted. There is a need to develope a simple design equation to establish the rotational stiffness effect, which could be easily quantified by comparing the theoretical critical stress equation for laminated composite plates with elastic restraints based on the Classical laminated plate theory. Through the parametric numerical studies, it is confirmed that there should clearly exist an increasing effect of local plate buckling strength due to the rotational stiffness by closed-section ribs. An applicable coefficient for practical design should be verified and proposed for future study. This study will contribute to the future study for establishing an increasing coefficient for the design strength and optimum design of U-rib stiffened plates.

• Journal of Korean Society of Steel Construction
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• v.27 no.4
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• pp.377-386
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• 2015

In this study, inelastic buckling strength of HSA800 steel columns were tested to investigate the applicability of current column curve (KBC2009, 2010 AISC-LRFD) to HSA800 steel. Key test variables included width-thickness ratio, plate-edge restraints, and column slenderness ratio. Specimens made of ordinary steel (SM490) were also tested for comparison purposes. All the concentrically loaded HSA800 specimens exhibited sufficient buckling strength compared to the current column curve, but some of SM490 specimens showed understrength. This difference can be explained by the lower residual stress present in HSA800 specimens.

• Journal of Korean Society of Steel Construction
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• v.20 no.3
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• pp.389-398
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• 2008

The current design practice of electric transmission tower is based on the allowable stress design. However, it is difficult to find the cause behind a transmission tower's collapse by the above design approach as the collapse is caused by large secondary deformations based on and geometrical nonlinear behavior.influence factor for the nonlinear behavior is mainly residual stress, initial imperfection and end restraints on members. In this study, the necessity of the nonlinear analysis is examined through the comparison between elastic ana the nonlinear analysis, a new analytical method (equivalent nonlinear analysis technique) is proposed. To confirm the reliability of the proposed method, the computed ultimate load of the transmission tower using the method was compared with that of the nonlinear finite element analysis. Effects of parameters, such as compressive force and the slenderness ratio of the brace member on the main post member, were investigated. The effective member length according to influential parameters was formulated in table form for practical purposes.