• 대한한의학방제학회지
• /
• 제25권4호
• /
• pp.483-497
• /
• 2017

Background : Behavioral stress has been suggested as one of the significant factors that is able to disrupt physiological systems and cause depression as well as changes in various body systems. The stressful events can alter cognition, learning, memory and emotional responses, resulting in mental disorders such as depression and anxiety. Results : We used a restraint stress model to evaluate the alteration of behavior and stress-related blood parameter. The animals were randomly divided into two groups of five animals each group. Furthermore, we assessed the change of body weight to evaluate the locomotor activity as well as status of emotional and anxiety in mice. After 7 days of restraint stress, the body weight had significantly decreased in the restraint stress group compared with the control group. We also observed stress-associated behavioral alterations, as there was a significant decrease in open field and forced swim test, whereas the immobilization time was significantly increased in the stress group compared to the control group. We observed the morphological changes of neuronal death and microglia by immunohistochemistry and western blot. In our study restraint stress did not cause change in neuronal cell density in the frontal cortex and CA1 hippocampus region, but there was a trend for an increased COX-2 and iNOS protein expression and microglia (CD11b) in brain, which is restraint stress. Conclusion : Our study, there were significant alterations observed in the behavioral studies. We found that mice undergoing restraint stress changed behavior, confirming the increased expression of inflammatory factors in the brain.

• Steel and Composite Structures
• /
• 제24권6호
• /
• pp.689-695
• /
• 2017

The constitutive relation is an important factor in analysis of confined concrete in composite structures. In order to propose a constitutive model for nonlinear analysis of confined concrete, lateral restraint mechanism of confined concrete is firstly analyze to study the generalities. As the foundation of the constitutive model, peak stress and peak strain is the first step in research. According to the generalities and the Twin Shear Unified Strength Theory, a novel unified equation for peak stress and peak strain are established. It is well coincident with experimental results. Based on the general constitutive relations and the unified equation for peak stress and peak strain, we propose a unified and convenient constitutive model for confined concrete with fewer material parameters. Two examples involved with steel tube confined concrete and hoop-confined concrete are considered. The proposed constitutive model coincides well with the experimental results. This constitutive model can also be extended for nonlinear analysis to other types of confined concrete.

• 대한한의학방제학회지
• /
• 제30권2호
• /
• pp.85-93
• /
• 2022

Objectives : This study investigated anti-inflammatory effects of Nardotidis seu Sulculii Concha water extract (NSCE) against restraint-induced stress. Methods : In vivo, NSCE was orally administered to male white mice at concentrations of 250 mg/kg and 500 mg/kg for 3 days, and then restraint-induced stress was induced for 6 hours. The level of liver damage was measured by serum aspartate aminotransferase (AST), alanine aminotransferase (ALT) and lactate dehydrogenase (LDH). The stress-related hormones such as cortisol and corticosterone were measured by ELISA assay. Also, western blot analysis was performed to detect expression of mitogen-activated protein kinase (MAPK) and cyclooxygenase-2 (COX-2) proteins. Pathological changes were observed by hematoxylin and eosin (H&E) staining of the liver tissue, and Immunohistochemical (IHC) staining was performed to examine liver inflammation through macrophage infiltration. Results : The AST, ALT, LDH and the stress related hormones such as cortisol and corticosterone were significantly decreased in the NSCE treated group compared with stress group. In histological analysis, H&E staining of liver tissues did not detect the hepatic injury or damage in all groups. As a result of IHC staining, it was confirmed that infiltration of macrophages was increased in the stress-induced group, but decreased in the group treated with NSCE. The COX-2 and MAPK proteins expression was significantly increased by restraint-induced stress, but these proteins were decreased in the NSCE treated group. Conclusions : These results suggest that NSCE has the anti-inflammatory activity in restraint-induced stress model, and it is believed that NSCE can be used for the prevention of liver inflammation.

• 대한토목학회논문집
• /
• 제32권5A호
• /
• pp.317-325
• /
• 2012

본 연구에서는 초고성능 섬유보강 콘크리트(Ultra High Performance Fiber Reinforced Concrete, UHPFRC)의 구속 상태에서의 수축 거동을 평가하고자 국내 외에서 가장 보편적으로 사용되는 링-테스트(ring-test)를 이용하여 구속 수축 실험을 수행하였다. 특히, 다양한 구속도에서의 수축 거동을 평가하기 위하여 내부 강재 링의 두께와 내부 반경을 달리하여 실험을 수행하였으며, 자유 수축과 인장강도 실험을 수반하여 구속도 및 응력 이완, 수축 균열 가능성 등을 복합적으로 평가하였다. 실험 결과 내부 링의 두께가 증가할수록 내부 링의 평균 변형률과 잔류 인장응력은 감소하였으며, 반면에 구속도는 증가하는 경향을 보였다. 내부 링의 반경에 따라서는 변형률 및 잔류 인장응력, 구속도의 차이가 거의 없는 것으로 나타났다. 모든 시험체에서 잔류 인장응력이 인장강도에 비해 작은 것으로 나타났으며, 수축 균열은 발생하지 않았다. 지속적으로 작용하는 계면 구속 하중에 의해 탄성 수축 응력의 약 39~65%가 이완되는 것으로 나타났으며, 최대 이완 응력은 내부 링의 두께가 두꺼울수록 증가하는 것으로 나타났다. 마지막으로 본 연구에서는 비선형 회귀분석을 수행하여 재령에 따라 변하는 구속도를 예측하였으며, 실험 결과와 잘 일치하는 것으로 나타났다.

• Steel and Composite Structures
• /
• 제4권1호
• /
• pp.23-36
• /
• 2004

The inelastic lateral-distortional buckling of doubly-symmetric hot-rolled I-section beam-columns subjected to a concentric axial force and uniform bending with elastic restraint which produce single curvature is investigated in this paper. The numerical model adopted in this paper is an energy-based method which leads to the incremental and iterative solution of a fourth-order eigenproblem, with very rapid solutions being obtained. The elastic restraint considered in this paper is full restraint against translation, but torsional restraint is permitted at the tension flange. Hitherto, a numerical method to analyse the elastic and inelastic lateral-distortional buckling of restrained or unrestrained beam-columns is unavailable. The prediction of the inelastic lateral-distortional buckling load obtained in this study is compared with the inelastic lateral-distortional buckling of restrained beams and the inelastic lateral-torsional buckling solution, by suppressing the out-of-plane web distortion, is published elsewhere and they agree reasonable well. The method is then extended to the lateral-distortional buckling of continuously restrained doubly symmetric I-sections to illustrate the effect of web distortion.

• 대한수의학회지
• /
• 제44권4호
• /
• pp.607-613
• /
• 2004

Helicobacter pylori (H. pylori)-infection is an important pathogen of stomach cancer after chronic gastritis and ulceration in the stomach and duodenum. However, the virulences of H. pylori strains have not been well-defined between clinical isolates. This study was designed to establish water-immersion-restraint stress (WIRS) model in mongolian gerbil for comparison of pathogenicity of H. pylori strains. To determine an optimal duration time for WIRS model in gerbil, 5-week-old Mongolian gerbils were divided into different groups by WIRS duration time. After graded duration of WIRS, the macroscopic ulcer index (UI) was measured with the stomach and duodenum of sacrificed animal. There were no significant differences between male and female in same duration group. However, the UI increased significantly in a time-dependent fashion. The group of 6 hours-WIRS animals showed severe hemorrhage and ulceration in their stomach and duodenum. On the other hand, the very mild lesions induced in 2 hours-treated animals. Therefore, we determined an optimal duration time for WIRS model in gerbil as 4 hours. Thereafter, we evaluated whether this WIRS model in gerbil could be used as an useful tool for in vivo comparison of pathogenicity of H. pylori strains by enhancement of pathological severity in H. pylori-infected gerbils. Mongolian gerbils were divided into H. pyloriinfected and PBS-inoculated groups. Thereafter, they were divided again into 4 hours-WIRS and no WIRS subgroups. After treatment, the severity of pathological changes was evaluated in a same manner with previous duration-determining experiment. When the animals were exposed to WIRS, the UI was significantly higher in the infected group than in the uninfected group. These results suggested that the established gerbil-WIRS model in this study enhanced effectively the severity of pathogenic changes in the H. pylori-infected Mongolian gerbils and could be used as an useful tool for in vivo comparison of pathogenicity of H. pylori strains.

• Structural Engineering and Mechanics
• /
• 제4권4호
• /
• pp.383-396
• /
• 1996

In this paper, the behavior of plates partially restrained against rotation under stress gradient is investigated. As a first stage, an energy formulation is presented to model this boundary condition and a general expression is derived for the prediction of the elastic buckling of the plate under this general loading condition. The accuracy of the derived expression is compared numerically using the Galerkin method with other available data for the two limiting conditions of rotationally free and clamped boundaries. Results show that the prediction is within a 5% difference. The influence of rotational restraint and stress gradient upon the buckling load and the associated buckling mode is investigated. Numerical results show sensitivity of the buckling mode to the degree of rotational restraint and the variation of the buckling load with the stress gradient.

• Structural Engineering and Mechanics
• /
• 제55권1호
• /
• pp.1-27
• /
• 2015

This article presents a theoretical parametric analysis on the ultimate torsional behaviour of axially restrained reinforced concrete (RC) beams. This analysis is performed by using a computing procedure based on a modification of the Variable Angle Truss Model. This computing procedure was previously developed to account for the influence of the longitudinal compressive stress state due to the axial restraint conditions provided by the connections of the beams to other structural members. The presented parametric study aims to check the influence of some important variable studies, namely: torsional reinforcement ratio, compressive concrete strength and axial restraint level. From the results of this parametric study, nonlinear regression analyses are performed and some design charts are proposed. Such charts allow to correct the resistance torque of RC beams (rectangular sections with small height to width ratios) to account for the favorable influence of the axial restraint.

• Biomolecules & Therapeutics
• /
• 제6권3호
• /
• pp.232-241
• /
• 1998

This study was done to investigate whether cholic acid derivatives have anti-stress activity in various stress models. Two cholic acid derivatives, ursodeoxycholic acid (UDCA) and tauroursodeoxycholic acid (WDCA), were used. physical, psychological, chemical and environmental stress models were performed. Adrenal weight, serum glucose levels and ALP activity were elevated in restraint stress model, but this elevation was prevented by UDCA treatment. Moreover, UDCA and TUDCA inhibited exploratory and spontaneous movements in oscillation stress model. In alcohol-induced stress model, TUDCA improved rotarod performance. UDCA and TUDCA significantly reduced the involution of lymphoid organs and the increment of WBC counts in cold stress model. These findings suggest that choric acid derivatives have antistress effects in various stress models.

• 한국콘크리트학회:학술대회논문집
• /
• 한국콘크리트학회 2001년도 가을 학술발표회 논문집
• /
• pp.641-646
• /
• 2001

In early-age concrete, volumetric deformations due to thermal expansion and moisture transfer are restrained by various boundary conditions, and then restraint stresses occur in proportion to developed stiffness. With increase of the age, these stresses are gradually relieved by significant relaxation behavior of early-age concrete. Therefore, it is necessary to consider the stress relaxation in order to analyze the behavior of early-age concrete more accurately. In this paper, we propose a unified algorithm which combines a relaxation model with hydration model, heat conduction model, micropore structure formation model, moisture diffusion model and mechanical properties development model and develop a finite element program based on the algorithm. The program is applied to evaluate stress development if a temperature-stress test machine (TSTM) specimen and a massive concrete structure, and then validity of the program is discussed and evaluated.