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http://dx.doi.org/10.14374/HFS.2022.30.2.85

Effect of Nardotidis seu Sulculii Concha water extract (NSCE) on liver damage and depression in restraint-induced stress model  

Kim, Min-Jung (Department of Veterinary Physiology, College of Veterinary Medicine, Gyeongsang National University)
Oh, Tae-woo (Korean Institute of Oriental Medicine Technology Application Center)
Do, Hyun-joo (Daegu-gyeongbuk Medical innovation foundation)
Kim, Kwang-yeon (Korean Institute of Oriental Medicine Technology Application Center)
Yang, Joo-hye (Korean Institute of Oriental Medicine Technology Application Center)
Son, Jae-Dong (Department of Veterinary Physiology, College of Veterinary Medicine, Gyeongsang National University)
Yang, Ye-jin (Department of Veterinary Physiology, College of Veterinary Medicine, Gyeongsang National University)
You, Young-Zoo (Department of Veterinary Physiology, College of Veterinary Medicine, Gyeongsang National University)
Kim, Woo-Hyun (Department of Veterinary Epidemiology College of Veterinary Medicine, Gyeongsang National University)
Kang, Seung-Ho (Daegu Haany University)
Lee, Dong-ho (Department of Anaesthesiology and Pain Medicine, Busan Paik Hospital, Inje University)
Ki, Seung-hee (Department of Anaesthesiology and Pain Medicine, Busan Paik Hospital, Inje University)
Kim, Young-Woo (School of Korean Medicine, Dongguk University)
Park, Kwang-Il (Department of Veterinary Physiology, College of Veterinary Medicine, Gyeongsang National University)
Publication Information
Herbal Formula Science / v.30, no.2, 2022 , pp. 85-93 More about this Journal
Abstract
Objectives : This study investigated anti-inflammatory effects of Nardotidis seu Sulculii Concha water extract (NSCE) against restraint-induced stress. Methods : In vivo, NSCE was orally administered to male white mice at concentrations of 250 mg/kg and 500 mg/kg for 3 days, and then restraint-induced stress was induced for 6 hours. The level of liver damage was measured by serum aspartate aminotransferase (AST), alanine aminotransferase (ALT) and lactate dehydrogenase (LDH). The stress-related hormones such as cortisol and corticosterone were measured by ELISA assay. Also, western blot analysis was performed to detect expression of mitogen-activated protein kinase (MAPK) and cyclooxygenase-2 (COX-2) proteins. Pathological changes were observed by hematoxylin and eosin (H&E) staining of the liver tissue, and Immunohistochemical (IHC) staining was performed to examine liver inflammation through macrophage infiltration. Results : The AST, ALT, LDH and the stress related hormones such as cortisol and corticosterone were significantly decreased in the NSCE treated group compared with stress group. In histological analysis, H&E staining of liver tissues did not detect the hepatic injury or damage in all groups. As a result of IHC staining, it was confirmed that infiltration of macrophages was increased in the stress-induced group, but decreased in the group treated with NSCE. The COX-2 and MAPK proteins expression was significantly increased by restraint-induced stress, but these proteins were decreased in the NSCE treated group. Conclusions : These results suggest that NSCE has the anti-inflammatory activity in restraint-induced stress model, and it is believed that NSCE can be used for the prevention of liver inflammation.
Keywords
Anti-Inflammation; restraint-induced stress; liver; Nardotidis seu Sulculii Concha;
Citations & Related Records
Times Cited By KSCI : 4  (Citation Analysis)
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