• The Korean Journal of Physiology and Pharmacology
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• v.20 no.5
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• pp.467-476
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• 2016

In the present study, we examined the effect of pertussis toxin (PTX) administered centrally in a variety of stress-induced blood glucose level. Mice were exposed to stress after the pretreatment of PTX (0.05 or 0.1 mg) i.c.v. or i.t. once for 6 days. Blood glucose level was measured at 0, 30, 60 and 120 min after stress stimulation. The blood glucose level was increased in all stress groups. The blood glucose level reached at maximum level after 30 min of stress stimulation and returned to a normal level after 2 h of stress stimulation in restraint stress, physical, and emotional stress groups. The blood glucose level induced by cold-water swimming stress was gradually increased up to 1 h and returned to the normal level. The intracerebroventricular (i.c.v.) or intrathecal (i.t.) pretreatment with PTX, a $G_i$ inhibitor, alone produced a hypoglycemia and almost abolished the elevation of the blood level induced by stress stimulation. The central pretreatment with PTX caused a reduction of plasma insulin level, whereas plasma corticosterone level was further up-regulated in all stress models. Our results suggest that the hyperglycemia produced by physical stress, emotional stress, restraint stress, and the cold-water swimming stress appear to be mediated by activation of centrally located PTX-sensitive G proteins. The reduction of blood glucose level by PTX appears to due to the reduction of plasma insulin level. The reduction of blood glucose level by PTX was accompanied by the reduction of plasma insulin level. Plasma corticosterone level up-regulation by PTX in stress models may be due to a blood glucose homeostatic mechanism.

• The Korean Journal of Physiology and Pharmacology
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• v.3 no.6
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• pp.555-563
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• 1999

It is well known that stress induces analgesia. This study was designed to demonstrate the stress-induced analgesia by employing hemorrhage and restraint and to investigate its mechanism and sex difference. The degree of pain was assessed by measuring the magnitude of jaw opening reflex produced by a noxious electrical stimulation in the dental pulp and by measuring the latency to withdraw the tail from a heat ray. Restraint showed an antinociceptive response. A significant increase in pain threshold on bleeding was shown and the increase was larger in male group than in female group. The tail flick latency (TFL) on bleeding after AVP antagonist injection into the ventricle was decreased and the decrease was greater in male rats than in female rats. Castration resulted in a significant reduction of TFL. This effect was reversed by treatment with sex hormones. TFL was decreased during hemorrhage in castrated rats. This response was opposite to that in non-castrated rats. TFL was further decreased during hemorrhage after infusion of AVP antagonist, and there was a significant sex difference. These results suggest that both restraint and hemorrhage produce an antinociception and that, in hemorrhage-induced analgesia, AVP and sex hormones may play an important role and male rats show a greater analgesic response.

• Journal of Oral Medicine and Pain
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• v.38 no.4
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• pp.291-298
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• 2013

On this study, we treated rats with restraint stress, and observed the changes with an optical microscope. Within the salivary gland tissue, we measured cell apoptosis cycle evaluation which show positive reaction on TUNEL assay, and compared within the groups. For this study, 18 rats were divided into 3 groups; 1) 2 rats of group I were selected as a normal control. 2) 2 rats of group II, as a experimental control were placed in the restraint cone for 2 hours 3) 14 rats of group III were placed in the restraint cone for 2 hours once a day. The rats were sacrificed immediately (group II, as a experimental control), 1, 2, 3, 4, 5, 6 and 7days after application of the stress and the both parotid glands were excised. The conclusions follow. 1. 5 days after giving an confining stress to the parotid gland of Rats, we can observe the hypotropy and pus and inflammation of Rat parotid gland acinar cells, and after 7 days, we can see a cell apoptosis. 2. Through the In situ DNA end labeling assay and TUNEL dye, on serous glands, benign tumor cell increased with statistically significant result after 5 days from confining stress. And the index shows maximum value on 7th days, which is same result with histological opinion. Therefore, our study shows that a cell apoptosis can be induced by restraint stress on salivary gland tissue, and we think more study should be accomplished about the cell signaling pathway in the future.

• Journal of Nutrition and Health
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• v.28 no.4
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• pp.291-297
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• 1995

This study aimed to verify the nutritional and curative effects of protein hydroysate in rats model with gastric ulcer induced by restraint and water-immersion stress. Sprague-Dawley, famale rats weighing approximtely 200g were forced in 5$\times$5$\times$15cm plexiglas cage. The restraint and water immersion stress was carried at 20$\pm$2$^{\circ}C$ for 8-hours. After stress 4 kinds of diets(10% casein, 20% casein, 10% casein hydrolysate, 20% casein hydrolysate) were given for 5 days. In the gastric ulcer rats model, the growth, gastric emptying rate, trypsin activity in gastrointestinal content, plasma total protein, albumin, $\alpha$-amino-N, UUN, creatinine and hydroxyproline of the urine and nitrogen retention were analyzed for nutritional effects of dietary nitrogen levels(10%, 20%) and sources (casein, casein hydrolysate). The results were as follows ; In gastric ulcer rats model, severeness of ulcer, plasma protein, gastric emptying rate, nitrogen retention rate were not different between 20% casein-fed group and 20% casein hydrolysatefed group. But 10% casein hydrolysate-fed group had more curative group. The casein hydrolysate diet-fed group was lower trysin activity in small intestianl content than the casein-fed group, at both casein level(10%, 20%). Finally at 20% levels, there was no difference between casein and casein hydrolysate diet, but 10% level, casein hydrolysate diet was more curative of ulcer than casein diet in gastric ulcer rat model. The results of this study provide useful information concerning diet therapy for the patients with gastrointestinal diseases and the field of enteral diet materials.

• Steel and Composite Structures
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• v.4 no.1
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• pp.23-36
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• 2004

The inelastic lateral-distortional buckling of doubly-symmetric hot-rolled I-section beam-columns subjected to a concentric axial force and uniform bending with elastic restraint which produce single curvature is investigated in this paper. The numerical model adopted in this paper is an energy-based method which leads to the incremental and iterative solution of a fourth-order eigenproblem, with very rapid solutions being obtained. The elastic restraint considered in this paper is full restraint against translation, but torsional restraint is permitted at the tension flange. Hitherto, a numerical method to analyse the elastic and inelastic lateral-distortional buckling of restrained or unrestrained beam-columns is unavailable. The prediction of the inelastic lateral-distortional buckling load obtained in this study is compared with the inelastic lateral-distortional buckling of restrained beams and the inelastic lateral-torsional buckling solution, by suppressing the out-of-plane web distortion, is published elsewhere and they agree reasonable well. The method is then extended to the lateral-distortional buckling of continuously restrained doubly symmetric I-sections to illustrate the effect of web distortion.

• Proceedings of the Korean Geotechical Society Conference
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• 2002.03a
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• pp.611-618
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• 2002

In this study the CGS as an injection method by low slump mortar was performed the pilot test to confirm the applicability of this method and the effectiveness of settlement restraint. From the results, there has been concluded the construction control standard such as an institutional diameter, space, depth, injection materials, Infection pressure etc. Also, there has been estimated the ground improvement effectiveness which has resulted from the field investigation and consolidation test etc. From the results, in the adjacent ground the CGS, generally, has been confirmed to in-crease ground strength to improve the consolidation characteristic obtained from the field investigation and consolidation test. Especially, the CGS which performed the larger stiffness to the ground has been concluded that the settlement restraint to the ground complicates the ground effect which Increases the bearing capacity and stress assignment. So, the CGS can be considered as an effective method to increase the bearing capacity as well as the settlement restraint of soft ground.

• Journal of Korean Biological Nursing Science
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• v.22 no.4
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• pp.279-287
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• 2020

Purpose: Some of the adolescent drinks more sugar-sweetened beverages. However, there is little evidence on the effect of eating behavior on emotional state and neurochemical changes under stress, especially on the levels of typical inhibitory neurotransmitters and gamma-aminobutyric acid. This article demonstrates that sucrose or saccharin drink reduces stress-related behavior responses and GABAergic deficits in adolescent rats. Methods: We randomly assigned 7-weeks-old Sprague-Dawley male rats to three groups: control group (Control), restraint stress only group (Stress), and restraint stress with unrestricted access to saccharin solution (Saccharin) and sucrose solution (Sucrose) as a positive control. We evaluated both anxious and depressive moods using an open field test and forced swim test, respectively. Using western blot analyses, the expression of a GABA-synthesizing enzyme, glutamate decarboxylase-67 (GAD67) and GABAergic markers, including calbindin and parvalbumin was assessed in the prefrontal cortex and the amygdala. Results: We found that both the drinks alleviated anxiety and depressive moods, induced significant attenuation in GAD67 level, and reduced calbindin level under stress in the prefrontal cortex and the amygdala. Conclusion: The results provide an understanding of the effect of sucrose or saccharin drink on stress-related responses. We propose the consumption of sweet drinks as a plausible strategy to alleviate stress-related alterations in adolescents.

• Structural Engineering and Mechanics
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• v.5 no.1
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• pp.105-113
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• 1997

This paper proposes a model for simulating concrete shrinkage taking into account aggregate restraint. In the model, concrete is regarded as a two-phase material based on shrinkage property. One is paste phase which undergoes shrinkage. Another is aggregate phase which is much more volumetrically stable. In the concrete, the aggregate phase is considered to restrain the paste shrinkage by particle interaction. Strain compatibility was derived under the assumption that there is no relative macroscopic displacement between both phases. Stresses on both phases were derived based on the shrinking stress of the paste phase and the resisting stress of the aggregate phase. Constitutive relation of paste phase was adopted from the study of Yomeyama, K. et al., and that of the aggregate phase was adopted from the author's particle contact density model. The equation for calculating concrete shrinkage considering aggregate restraint was derived from the equilibrium of the two phases. The concrete shrinkage was found to be affected by the free shrinkage of the paste phase, aggregate content and the stiffness of both phases. The model was then verified to be effective for simulating concrete shrinkage by comparing the predicted results with the autogeneous and drying shrinkage test results on mortar and concrete specimens.

• Korean Journal of Veterinary Research
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• v.41 no.4
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• pp.597-602
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• 2001

This study was carried out to investigate the effectiveness of active biotelemetry to monitor stress response, and the effects of acute stressor (raising a hind foot of a standing animal) on body temperature (BT) and heart rate (HR) in comparison heifer group (n=4) with cow group (n=3). Both parameters were recorded by active telemetry. Cortisol assay was used to compare the result of active biotelemetry. The stressor consisted in restraining the cows for hoof treatments. Body temperature and heart rate were measured at 0, 10, 20, 30, 40, 50, 60, 90, 120, 180 and 360 minutes during the experiments, and also cortisol assay at 0, 0.5, 1, 2, 3, 12 and 24 hours. Base line for these animals was set in there accustomed environment at -24hrs and -30 mins before the beginning of experiments. The results obtained in this study were as follows: 1. In the BT, heifer group showed no significant difference from cow group, but were observed slightly higher BT than cow group during restraint stress for 30 minutes. 2. The both groups showed directly significant increase of HR immediately after the beginning of the restraint stress (P<0.01), but in the required time for the recovery of HR after finishing the stress, heifer group was 40 minutes and cow group 60 minutes. 3. In the cortisol assay, the both groups increased from 30 to 120 minutes after the beginning of experiment (i.e, from 0 to 90 minutes after finishing the restraint stress), showing significant difference from before the beginning of the stress (P<0.01), but heifer group showed great increase of the cortisol level from 30 to 60 minutes after the beginning of the stress, while cow group was observed at 60 minutes. In conclusion, we suppose that care is required to decrease psychological stress in cattle when they suffer from a series of procedures for diagnosis and treatment, especially in heifers rather than in cows. Also BT and HR obtained by active biotelemetry are reliable indicators of stress in cattle.

• Structural Engineering and Mechanics
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• v.4 no.4
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• pp.383-396
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• 1996

In this paper, the behavior of plates partially restrained against rotation under stress gradient is investigated. As a first stage, an energy formulation is presented to model this boundary condition and a general expression is derived for the prediction of the elastic buckling of the plate under this general loading condition. The accuracy of the derived expression is compared numerically using the Galerkin method with other available data for the two limiting conditions of rotationally free and clamped boundaries. Results show that the prediction is within a 5% difference. The influence of rotational restraint and stress gradient upon the buckling load and the associated buckling mode is investigated. Numerical results show sensitivity of the buckling mode to the degree of rotational restraint and the variation of the buckling load with the stress gradient.