• Molecules and Cells
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• 제38권9호
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• pp.796-805
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• 2015

Gintonin is a novel ginseng-derived lysophosphatidic acid (LPA) receptor ligand. Oral administration of gintonin ameliorates learning and memory dysfunctions in Alzheimer's disease (AD) animal models. The brain cholinergic system plays a key role in cognitive functions. The brains of AD patients show a reduction in acetylcholine concentration caused by cholinergic system impairments. However, little is known about the role of LPA in the cholinergic system. In this study, we used gintonin to investigate the effect of LPA receptor activation on the cholinergic system in vitro and in vivo using wild-type and AD animal models. Gintonin induced $[Ca^{2+}]_i $ transient in cultured mouse hippocampal neural progenitor cells (NPCs). Gintonin-mediated $[Ca^{2+}]_i $ transients were linked to stimulation of acetylcholine release through LPA receptor activation. Oral administration of gintonin-enriched fraction (25, 50, or 100 mg/kg, 3 weeks) significantly attenuated scopolamine-induced memory impairment. Oral administration of gintonin (25 or 50 mg/kg, 1 2 weeks) also significantly attenuated amyloid-${\beta}$ protein ($A{\beta}$)-induced cholinergic dysfunctions, such as decreased acetylcholine concentration, decreased choline acetyltransferase (ChAT) activity and immunoreactivity, and increased acetylcholine esterase (AChE) activity. In a transgenic AD mouse model, long-term oral administration of gintonin (25 or 50 mg/kg, 3 months) also attenuated AD-related cholinergic impairments. In this study, we showed that activation of G protein-coupled LPA receptors by gintonin is coupled to the regulation of cholinergic functions. Furthermore, this study showed that gintonin could be a novel agent for the restoration of cholinergic system damages due to $A{\beta}$ and could be utilized for AD prevention or therapy.

• 한국환경생태학회지
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• 제30권1호
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• pp.104-109
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• 2016

정부는 해양환경복원과 수산자원을 회복하기 위해 1971년부터 2014년까지 약 44년 동안 222,627ha의 인공어초어장을 조성하였다. 그러나 인공어초 설치사업은 증가되고 있는 반면 아직까지 설치된 어초의 효과에 대한 연구는 부족한 실정이다. 이에 본 연구에서는 포항시 장길리 해역에 설치된 뿔삼각형어초에 부착 서식하는 저서동물의 시간적 군집변화를 살펴보기 위해 2003년 포항시 장길리 해역 수심 5~7m에 설치된 뿔삼각형어초를 대상으로 2009년 2월부터 2011년 8월까지 총 4회에 걸쳐 어초에 부착된 저서동물의 군집을 분석하였다. 그 결과 우점하는 분류군은 연체동물이고 우점종은 바다방석고둥인 것으로 나타났다. 또한 출현종들은 대부분 시공간의 변화에 따라 달라지는 양상을 보였다. 따라서 이 연구를 통해 시설해역과 목적하는 종의 특성에 적합한 인공어초 개발 연구, 사후관리에 관한 이용, 관리제도 제정 등에 활용될 것으로 기대한다.

• 한국패류학회지
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• 제31권4호
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• pp.307-314
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• 2015

본 조사는 2013년 6월부터 8월까지 금강 상류에서 멸종위기야생생물 I급인 두드럭조개 (Lamprotula coreana)의 서식지를 조사하였다. 서식지 평가를 위하여 하천의 물리적 구조인 여울, 소 및 유수역이 모두 포함된 다양한 서식환경에서 서식상태를 조사하였으며, 하천단면, 수심, 유속, 하상재료 및 서식지 유형 등을 현장에서 측정 하였다. 서식 확인된 두드럭조개는 크기, 무게 및 연령을 기록하고 서식지에 재살포하였고, 서식지적합도지수 (Habitat suitability index, HSI)와 PHABSIM (physical habitat simulation system)에 의한 최적 생태유량 산정을 위하여, 각각의 채집 장소에서 수심, 유속 및 하상재료 등을 기록하였다. 서식지 평가는 수위, 유량 및 하천단면의 현장조사 결과와 두드럭조개의 HSI를 PHABSIM에 적용하여 가용서식지면적 (weighted usable area, WUA)과 유량의 관계 곡선을 작성하였다. 금강 상류에서 채집된 두드럭조개는 각장 $73.1{\pm}18.4mm$ 및 전중 $131.6{\pm}72.3g$ 이었으며, 연령은 2-7년생까지 서식하는 것으로 확인되었다. 두드럭조개의 HSI는 수심 0.4-0.5 m, 유속 0.3-0.5 m/s, 하상재료는 모래-호박돌로 나타났다. PHABSIM에 의해 모의된 최적유량은 2.1 cms였으며, WUA는 $3,730m^2$/1000 m로 나타났다. 두드럭조개의 서식지 평가 결과는 최근 각종 하천 공사 및 생태적 교란 등으로 서식 범위가 감소하고 있는 시점에서 서식지 복원 및 관리를 위한 기초자료로 활용될 수 있을 것이다.

• 대한치과보철학회지
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• 제34권2호
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• pp.266-276
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• 1996

Numerous factors are known to affect the accuracy of elastomeric impression materials. Factor often overlooked is the quality of the bond between putty and wash during corrective reline impression technique. The putty-wash bond strength must be strong enough to over-come the local stress at putty-wash interface. It is not always possible to avoid saliva contamination in making corrective wash impres-sion. And putty preliminary impression material con be used as a template for provisional restoration. Human saliva and the residual monomer of autopolymerizing acrylic resin are thought to affect the bond strength and the failure type. This study examined the effect of contaminants like human saliva, and residual resin monomer on the putty-wash bond strength and the effectiveness of treatment. 1. Of the tested three brands of Vinyl Polysiloxane impession meterial, Express Exhibited the greatest bond strength followed by Eamix and Perfect showed the lowest putty-wah bond strength. 2. Coating the putty substrates with human saliva did not produce decreased failure load in all the breands of Vinyl Polysiloxane impression meterail. 3. Of the three brands of VPS impression material that were exposed to methhylmethacry-late resin(Jet), only the putty-wash bond strength of the Perfect group diminished signifi-cantly. Moreover, all the specimens from group C of Perfect exhibited adhesive failure. 4. Exposing the substrates to ethylmethacrylate resin(SNAP. diminished the putty-wash bond strength significantly. With Perfect and Examix, failure occurred cohesively through the light-body, whereas with Express, failure occurred adhesive-cohesively. 5. Removing approximately 1mm thickness of the contaminated putty interface was the most effective treatment in countering the undesirable effect caused by residual resin monomer. The putty-wash bond strength of the groups that were treated with 1mm even putty reduction was not significantly different from those of control groups. With Perfect and Examix, cleaning the specimens with gauze soaked in 70% isopropyl alcohol increased the putty-wash bond strength, but was not as effective as 1mm even reduction of contaminated putty substrates. With Express, 70% isoproryl alcohol treatment exhibi0ted comparable putty-wash bond strength to that of control group.

• 대한치과보철학회지
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• 제40권3호
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• pp.296-305
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• 2002

The main disadvantage of cement-retained implant restorations is their difficulty in retrievability. Advocates of cemented implant restorations frequently state that retrievability of the restoration can be maintained if a provisional cement is used. The purpose of this study was to find the optimal properties of provisional luting cements and the surface treatment of abutments in single implant abutment system. 30 prefabricated implant abutments, height 8mm, diameter 6mm, 3-degree taper per side, with light chamfer margins were obtained. Three commercially available provisional luting agents which were all zinc oxide eugenol type ; Cavitec, TempBond and TempBond NE were evaluated. No cement served as the control. TempBond along with vaseline, a kind of petrolatum (2:1 ratio) was also evaluated. Ten out of thirty abutments were randomly selected and abutment surfaces were sandblasted with $50{\mu}m$ aluminum oxide. Another ten abutments were sandblasted with $250{\mu}m$ aluminum oxide. A vertical groove, 1 mm deep and 5mm long was cut in each twenty abutments. Ten of them were sandblasted with $50{\mu}m$ aluminum oxide. The full coverage casting crowns were cemented to the abutments with the designated provisional luting agent. Specimens were stored in distilled water at $37^{\circ}C$ for 24 hours. Each specimen was attached to a universal testing machine. A crosshead speed of 0.5mm/min was used to apply a tensile force to each specimen. Within the limitations of this in vitro study, the following conclusions were drawn: 1. Tensile bond strength of provisional luting cements in no surface treatment decreased with the sequence of TempBond NE, TempBond, Cavitec, TempBond with vaseline, no cement. 2. Tensile bond strength more increased by surface treatment. Sandblasting with $250{\mu}m$ aluminum oxide exhibited the highest tensile bond strength in the abutment cemented with TempBond NE and sandblasting with $50{\mu}m$ aluminum oxide exhibited the highest tensile bond strength in cemented with TempBond. 3. In the aspect of a groove formation, tensile bond strength significantly increased in TempBond with vaseline only and the others had no significant effect on tensile bond strength.

• 복식
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• 제18권
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• pp.373-381
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• 1992

Woman in Choson era had various kind of accdssories like rings, ear ring, ornamentak hairpins and so on. Among them their favorite was the Norigae, the pendent trinket. With the embroidered ornament at its center, beads and knots were attached to the upper and lower part, and it was hung with a tassel. It was carried on the string of the Chogori. norigae oreginated in waist string of Three Countries era(Sills, Paekje and Koguryo) ; had been changed with the development of fashion, embroidery and the influence of Buddhistic culture. The differences between Embroidered Norigae and Norigae are following. If the ornament at its center is made of such jewels as gold, silver, jade and the like, it is called Norigae. But if the ornament at its center is made of the embroidered Norigae. Such a Embroidered Norigae in which perfume was contained, was as a perfume box or a perfume bag and was carried on the strings of Chogori by Danjak(single work). The Differences between ancient Embroidered Norigae of late Choson era and modern Embroidered Norigae are Following; 1) The type of main body-the embroidered ornament at its center was mainly embroidered with the shape of animals in both of ancient Embroidered Norigae was usually formes like a bat and that of modern Embroidered Norigae like butterfly. 2) plants were adapted as the main design of ancient Embroidered Norigae, but plants and animals as that of modern Embroidered Norigae. 3) The colors of the background and the design are usually red in both ancient and modern Embroidered norigae. 4) The tassels of ancient Embroidered Norigae were mode in the shape of srick and a strawberry. but the tassels of modern Embroidered Norigae were mainly made in the shape of a stick. 5) Nowadays the beauty of external form is considered more important while the symbol of main body was considered more important in Choson era. 6) The role of Thidon in the composition of Embroidered Norigae is declined and a lot of main bodies are used in modern Embroidered Norigae. 7) Modern Korean people are apt to choose Embroidered Norigae depending upon the design of main body and the traditional Korean beauty. 8) Modern people who desire to get new thing, try to make the design of main body various. As their cognition of Korean tradition is of the increase, the restoration of ancient Embroidered Norigae to its original form has been accom

• 한국치위생학회지
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• 제11권1호
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• pp.127-136
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• 2011

Objectives : The writer aimed to examine the dynamic aspect on damage and to be conducive to the swift cure according to right treatment to educate prevention of injury through surveying on the number of the damaged teeth given injury, tooth kind, type in damage, place and cause for being damaged, frequency by month and by time level, and location of the damaged tooth. Methods : Targeting 343 teeth of receiving injury in 201 patients who visited the department of pediatric dentistry, the department of preservation, and the oral and maxillofacial surgery in a dental hospital in Cheonan city from April 2007 to April 2009, by having the dental injury as the main reason, the following conclusions were obtained. Results : 1. Deciduous teeth were indicated to be the highest in imperfect luxation with 60.7%, and were indicated to be in order of crown fracture with 25.5%, root fracture with 12.4%, and perfect luxation with 1.4%. The permanent teeth were indicated to be the highest in crown with 58.1%, and were indicated to be in order of imperfect luxation with 27.3%, root fracture with 13.1%, and perfect luxation with 1.5%(p<0.001). 2. In case of deciduous teeth for a place with damage, the home was indicated to be the highest with 31.7%. The permanent teeth were indicated to be the highest(p<0.001) in the road and stairs with 40.4%. As to a cause for damage, both deciduous and permanent teeth were indicated to be the highest(p<0.001) in a cause for falling with 53.1% and 30.8%, respectively. 3. As for a treatment method, in case of deciduous teeth, the close observation was indicated to be the highest with 46.9%. In permanent teeth, the resin restoration was indicated to be the highest(p<0.001) with 22.2%. Dental injuries in deciduous teeth and permanent teeth are showing diverse aspects. Conclusions : It is important to arrange guidelines on cure of the damaged teeth by injury through continuing a dynamic research on these aspects. Also, the damage in deciduous teeth may have direct and indirect influence upon growth in successional permanent teeth. Thus, through careful treatment, the injury needs to be minimized. It is considered to be likely necessary for a right coping method when injury occurs, and above all, for enough prior education so that injury cannot occur.

• Journal of Microbiology and Biotechnology
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• 제30권6호
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• pp.937-945
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• 2020

N-acyl-homoserine lactone (AHL)-mediated quorum sensing (QS) plays a major role in development of biofilms, which contribute to rise in infections and biofouling in water-related industries. Interference in QS, called quorum quenching (QQ), has recieved a lot of attention in recent years. Rhodococcus spp. are known to have prominent quorum quenching activity and in previous reports it was suggested that this genus possesses multiple QQ enzymes, but only one gene, qsdA, which encodes an AHL-lactonase belonging to phosphotriesterase family, has been identified. Therefore, we conducted a whole genome sequencing and analysis of Rhodococcus sp. BH4 isolated from a wastewater treatment plant. The sequencing revealed another gene encoding a QQ enzyme (named jydB) that exhibited a high AHL degrading activity. This QQ enzyme had a 46% amino acid sequence similarity with the AHL-lactonase (AidH) of Ochrobactrum sp. T63. HPLC analysis and AHL restoration experiments by acidification revealed that the jydB gene encodes an AHL-lactonase which shares the known characteristics of the α/β hydrolase family. Purified recombinant JydB demonstrated a high hydrolytic activity against various AHLs. Kinetic analysis of JydB revealed a high catalytic efficiency (k_cat/K_M) against C4-HSL and 3-oxo-C6 HSL, ranging from 1.88 x 10⁶ to 1.45 x 10⁶ M^-1 s^-1, with distinctly low K_M values (0.16-0.24 mM). This study affirms that the AHL degrading activity and biofilm inhibition ability of Rhodococcus sp. BH4 may be due to the presence of multiple quorum quenching enzymes, including two types of AHL-lactonases, in addition to AHL-acylase and oxidoreductase, for which the genes have yet to be described.

• 보존과학회지
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• 제33권6호
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• pp.541-552
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• 2017

발굴된 모든 매장문화재는 보존조치의 필요성에 따라 문화재청장은 보존조치를 결정한다. 하지만 보존조치 결과에 따라 이전 복원된 유적의 관리에 대한 법적인 규정이 없는 상태로 인해 관리에 대하여 소홀한 실정이다. 이에 본 논문에서는 충청남도 지역을 대상으로 최근까지 보존조치되어 이전복원된 매장문화재의 보존현황 및 보존방안에 대하여 제시하였다. 충청남도에서 매장문화재 발굴 후 보존조치된 유적은 23개 유적 46개 유구가 확인되었다. 보존유형은 해체이전 27개 유구, 유구전사 14개 유구, 해체이전+유구전사 병행 2개 유구, 모형제작 3개 유구로 이루어졌다. 복원 설치된 유적의 지역별로는 부여군 9개 유적, 공주시 3개 유적으로 부여군과 공주시에 12개 유적 18개 유구가 집중적으로 분포되어 있고, 시대별로는 백제시대 유적이 16개 유적으로 약 70%를 차지하고 있었다. 보호유형은 노천형이 33개 유구로 약 72%를 차지하고 있는 것으로 확인되었다. 이전복원된 유적 관리 상태조사 결과 관리 주체가 명확한 박물관 등 공공기관은 관리를 위한 자체인력을 보유하고 있어 보존 관리가 잘 이루어지고 있었으나 관리 주체가 모호한 경우에는 관리 소홀로 인한 유구 손상이 심각한 것으로 나타났다. 이러한 문제점 해결을 위해 명확한 관리 주체의 명시, 주기적인 관리 및 점검, 이전 복원 방법에 대한 보존처리 방법 연구 등이 필요하다. 발굴된 매장문화재는 학술적 목적뿐만 아니라 홍보자료 및 전시자료로도 활용하고 있어 보다 철저한 관리를 통한 매장문화재의 활용이 필요하다고 판단된다.

• 환경생물
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• 제37권3호
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• pp.229-248
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• 2019

본 연구는 경상누층군 자암산의 관속식물상을 밝히고 주요 식물에 대해 조사하였다. 2017년 3월부터 10월까지 총 8회에 걸쳐 현지조사를 실시한 결과, 관속식물은 110과 325속 483종 8아종 35변종 2품종 2교잡종으로 총 530분류군이 확인되었다. 자암산은 온대 중부지역에 속하고 침엽수와 낙엽활엽수 혼합림으로 산지의 대부분은 소나무-참나무류가 우점하며, 2차림으로 구성된다. 한반도 고유종은 12분류군, IUCN 평가기준에 따른 적색목록식물은 17분류군, 식물구계학적 특정식물은 65분류군이 관찰되었다. 침입외래식물은 55분류군이며, 귀화율 10.4%, 도시화지수 17.2%로 나타났다. 자암산은 고유종, 희귀종을 포함하여 식물다양성이 높을 뿐만 아니라, 다양한 지형요소들이 주요 경관을 이루고 있다. 본 연구를 통해 조사지 내의 퇴적암벽, 건조한 능선, 계곡, 하천 등의 다양한 환경 요인이 종조성과 분포에 영향을 미치는 것으로 파악되었다. 또한 이 지역의 암석과 토양은 양지식물에 대한 특이적 환경을 제공하는 데 있어 주요한 요인으로 작용하였다.