• Journal of Animal Science and Technology
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• 제63권3호
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• pp.651-661
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• 2021

This study aimed to investigate whether breed, sex, and age affected temperament differently (more or less neophobic) in mature horses during a novel object test. The study included Jeju crossbred (n = 12, age = 9.42 ± 4.57 y), Thoroughbred (n = 15, age = 10.73 ± 3.09 y), and Warmblood horses (n = 12, age = 13.08 ± 3.55 y) with the females (n = 22, age = 11.36 ± 4.24 y) and geldings (n = 17, age = 10.65 ± 3.66 y). Jeju crossbreds (Jeju horse × Thoroughbred) are valuable considering their popular usage in Korea, but limited studies have explored temperament of Jeju crossbred horses. A trained experimenter touched the left side of the neck with a white plastic bag (novel object). The test ended when the horse stopped escape response and heart rate (HR) dropped to baseline. Behavioral score and escape duration were measured as behavioral variables. Multiple variables related to HR and heart rate variability (HRV) were measured to reflect emotional state. These included basal HR (BHR), maximum HR (MHR), delay to reach maximum heart rate (Time to MHR), standard deviation of beat-to-beat intervals (SDNN), root mean square of successive differences (RMSSD), and ratio of low to high frequency components of a continuous series of heartbeats (LF/HF). Statistics revealed that Thoroughbreds had significantly higher behavioral scores, and lower RMSSD than Jeju crossbreds (p < 0.05), suggesting greater excitement and fear to the novel object in Thoroughbreds. None of the behavioral or cardiac parameters exhibited sex differences (p < 0.05). Age was negatively correlated with SDNN and RMSSD (p < 0.05), indicating that older horses felt more anxiety to the novelty than younger horses. Thoroughbreds and females had distinct correlations between behavioral and HRV variables in comparison with other groups (p < 0.05), implying that escape duration might be a good indicator of stress, especially in these two groups. These results are expected to improve equine welfare, safety and utility, by providing insights into the temperament of particular horse groups, to better match reactivity levels with specific functions.

• Journal of Veterinary Science
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• 제22권1호
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• pp.9.1-9.11
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• 2021

Background: Scalding burn injuries can occur in everyday life but occur more frequently in young children. Therefore, it is important to develop more effective burn treatments. Objectives: This study examined the effects of bee venom (BV) stimulation on scalding burn injury-induced nociception in mice as a new treatment for burn pain. Methods: To develop a burn injury model, the right hind paw was immersed temporarily in hot water (65℃, 3 seconds). Immediately after the burn, BV (0.01, 0.02, or 0.1 mg/kg) was injected subcutaneously into the ipsilateral knee area once daily for 14 days. A von Frey test was performed to assess the nociceptive response, and the altered walking parameters were evaluated using an automated gait analysis system. In addition, the peripheral and central expression changes in substance P (Sub P) were measured in the dorsal root ganglion and spinal cord by immunofluorescence. Results: Repeated BV treatment at the 2 higher doses used in this study (0.02 and 0.1 mg/kg) alleviated the pain responses remarkably and recovered the gait performances to the level of acetaminophen (200 mg/kg, intraperitoneal, once daily), which used as the positive control group. Moreover, BV stimulation had an inhibitory effect on the increased expression of Sub P in the peripheral and central nervous systems by a burn injury. Conclusions: These results suggest that a peripheral BV treatment may have positive potency in treating burn-induced pain.

• 한국유기농업학회지
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• 제28권4호
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• pp.627-641
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• 2020

Intensive farming methods that do not guarantee animal welfare can induce stress in pigs. Stress, in turn, can reduce their disease resistance and influence their hormones and metabolites in such a manner that productivity is negatively affected. This study was conducted to compare the stress related factors and blood characteristics of pigs raised on conventional farms and those raised on animal welfare farms. We measured the levels of cortisol, epinephrine and norepinephrine, biochemical parameters in blood and glycogen, L-lactate and heat shock protein 70 (HSP70) in muscle, as physiological markers of indicating the stress in conventional farm pigs (Control, n=10) and animal welfare farm pigs (Welfare, n=10). We found that there was a significant difference in the albumin-globulin ratio (A/G ratio), as well as the albumin (ALB), blood urea nitrogen (BUN) and aspartate aminotransferase (AST) levels between the two farms. Epinephrine was significantly higher in conventional farm, while level of norepinephrine was higher in animal welfare farm. There was no significant difference in cortisol, which is known as a stress hormone, across the two groups of farms. Muscular glycogen content was significantly high in animal welfare farm pigs. While L-lactate tended to be low in the animal welfare farm pigs, the difference between them and the conventional farm cohorts was not significant. HSP70 showed high levels of expression in conventional farm. Thus, we suggested that blood parameter results showed a stress response in the livers of conventional farm, and that catecholamine hormones, glycogen, L-lactate and HSP70 can be used as physiological factors of assessing animal welfare.

• Wind and Structures
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• 제32권4호
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• pp.355-369
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• 2021

Shape optimization of tall buildings is an efficient approach to mitigate wind-induced effects. Several studies have demonstrated the potential of shape modifications to improve the building's aerodynamic properties. On the other hand, it is well-known that the cross-section geometry has a direct impact in the floor area availability and subsequently in the building's profitability. Hence, it is of interest for the designers to find the balance between these two design criteria that may require contradictory design strategies. This study proposes a surrogate-based multi-objective optimization framework to tackle this design problem. Closed-form equations provided by the Eurocode are used to obtain the wind-induced responses for several wind directions, seeking to develop an industry-oriented approach. CFD-based surrogates emulate the aerodynamic response of the building cross-section, using as input parameters the cross-section geometry and the wind angle of attack. The definition of the building's modified plan shapes is done adopting the reduced basis approach, advancing the current strategies currently adopted in aerodynamic optimization of civil engineering structures. The multi-objective optimization problem is solved with both the classical weighted Sum Method and the Weighted Min-Max approach, which enables obtaining the complete Pareto front in both convex and non-convex regions. Two application examples are presented in this study to demonstrate the feasibility of the proposed strategy, which permits the identification of Pareto optima from which the designer can choose the most adequate design balancing profitability and occupant comfort.

• 한국육종학회지
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• 제43권5호
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• pp.391-398
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• 2011

Physiological responses to salinity stress were evaluated in six rice genotypes differing in their tolerance to salinity at the seedling stage. Susceptible genotypes ('Dongjingbyeo', 'Hwayeongbyeo', and 'IR29') showed salt injury symptoms (mean 8.8) and higher visual score under salt stress than that of tolerant ones ('Pokkali', 'IR74009', and 'IR73571'). As salinity affects growth and physiological parameters, the six genotypes thus showed significant reduction because of salt stress. Tolerant Japonica/Indica bred lines ('IR74009', 'IR73571') showed lower reduction, 33.9%, 34.5%, and 50%, respectively, in plant seedling height, dry shoot weight and dry root weight than those of the susceptible Japonica varieties ('Dongjingbyeo', 'Hwayeongbyeo'), and the highest reduction under salt stress was observed in dry root weight, followed by dry shoot weight and seedling height, respectively. Shoot $Na^+$ concentration of IR74099 and IR73571 was lower than that of the susceptible varieties, 'Dongjinbyeo' and 'Hwayeongbyeo'. There were no significant differences among genotypes in root $Na^+$ concentration. Shoot $K^+$ concentration showed a reverse tendency compared to shoot $Na^+$ concentration. IR74009 and IR73571 had considerably lower ratio compared to 'Dongjinbyeo' and 'Hwayeongbyeo' in $Na^+/K^+$ ratio of their shoot and was not different the tolerant check, 'Pokkali'.

• 한국전산구조공학회논문집
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• 제31권6호
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• pp.283-291
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• 2018

본 연구는 지진에 저항하는 부재인 비보강 조적벽체로 구성된 건물의 내진성능평가에 활용되는 비선형 정적해석을 위한 비보강 조적벽체의 해석모델을 수립하고자 하였다. 본 연구의 해석모델은 비보강 조적벽체의 휨거동을 모사하기 위한 파이버 요소와 비보강 조적벽체의 전단에 대한 응답을 예측하기 위한 전단스프링 요소로 구성된다. 본 논문은 먼저 제안하고 있는 모델의 형상에 대해서 설명하고, 기존에 행해진 조적조 프리즘의 실험결과로부터 얻은 응력-변형률 곡선을 근거로 파이버와 전단스프링 요소의 물성치에 대한 결정 방법을 설명한다. 제시하고 있는 모델은 비선형 정적 해석결과와 다른 연구자들에 의해 수행된 실험결과를 비교하여 타당성을 검증한다. 해당 모델은 최대강도, 초기강성, 그리고 이들로부터 얻어지는 비보강 조적벽체의 하중-변위 곡선을 적절하게 모사하고 있다. 또한, 해석모델이 비보강 조적벽체의 파괴모드를 예측할 수 있는 것으로 나타난다.

• 한국응용과학기술학회지
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• 제35권4호
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• pp.1393-1406
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• 2018

본 연구는 3개의 운전변수(압력, 공기량, 운전시간)를 실험 설계하고 마이크로 버블의 종말부상속도(Terminal rise velocity)를 반응 값으로 하여 예측식 모델과 최적 조건을 수립하였다. 다항식 회귀분석을 통해 펌프의 압력($X_1$) 4.5bar, 공기량($X_2$) 3.3L/min 그리고 운전시간($X_3$)이 2.2min에서 종말상승속도(Terminal rise velocity)에 대한 최적값인 5.14 cm/min ($85.7{\mu}m/sec$)을 얻었다. 또한, 레이저 입자계수 측정장치를 이용하여 $2{\sim}5{\mu}m$ 및 $25{\sim}50{\mu}m$ 영역에서의 가장 높은 마이크로버블 직경크기 분포를 확인하였다.

• Environmental Engineering Research
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• 제23권4호
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• pp.474-484
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• 2018

The cleaning efficiency of reverse osmosis (RO) membranes inevitably fouled by organic foulants depends upon both chemical (type of cleaning agent, concentration of cleaning solution) and physical (cleaning time, flowrate, temperature) parameters. In attempting to determine the optimal procedures for chemical cleaning organic-fouled RO membranes, the design of experiments concept was employed to evaluate key factors and to predict the flux recovery rate (FRR) after chemical cleaning. From experimental results and based on the predicted FRR of cleaning obtained using the Central Composite Design of Minitab 17, a modified regression model equation was established to explain the chemical cleaning efficiency; the resultant regression coefficient ($R^2$) and adjusted $R^2$ were 83.95% and 76.82%, respectively. Then, using the optimized conditions of chemical cleaning derived from the response optimizer tool (cleaning with 0.68 wt% disodium ethylenediaminetetraacetic acid for 20 min at $20^{\circ}C$ with a flowrate of 409 mL/min), a flux recovery of 86.6% was expected. Overall, the results obtained by these experiments confirmed that the equation was adequate for predicting the chemical cleaning efficiency with regards to organic membrane fouling.

• Journal of Microbiology and Biotechnology
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• 제29권7호
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• pp.1117-1123
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• 2019

Control of pine wilt disease, which is caused by pine wilt nematode Bursaphelenchus xylophilus, is heavily dependent on the use of chemicals such as abamectin. Although such chemicals are highly effective, demands for alternatives that are derived preferentially from natural sources, are increasing out of environmental concerns. One of the challenges to discovery of alternative control agents is lack of fast and efficient screening method that can be used in a high-throughput manner. Here we described the development of colorimetric assay for the rapid and accurate screening of candidate nematicidal compounds/biologics targeting B. xylophilus. Contrary to the conventional method, which relies on laborious visual inspection and counting of nematode population under microscope, our method utilizes a redox dye that changes its color in response to metabolic activity of nematode population in a given sample. In this work, we optimized parameters of our colorimetric assay including number of nematodes and amount of redox dye, and tested applicability of our assay for screening of chemicals and biologics. We demonstrated that our colorimetric assay can be applied to rapid and accurate quantification of nematode viability/mortality in a nematode population treated with candidate chemicals/biologics. Application of our method would facilitate high-throughput endeavors aiming at finding environment-friendly control agents for deadly disease of pine trees.

• Steel and Composite Structures
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• 제31권6호
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• pp.575-589
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• 2019

The main focus of this study is to numerically investigate the influence of strong earthquake and tsunami-induced wave impact on the response and behavior of a cable-stayed steel bridge with large caisson foundations, by assuming that the earthquake and the tsunami come from the same fault motion. For this purpose, a series of numerical simulations were carried out. First of all, the tsunami-induced flow speed, direction and tsunami height were determined by conducting a two-dimensional (2D) tsunami propagation analysis in a large area, and then these parameters obtained from tsunami propagation analysis were employed in a detailed three-dimensional (3D) fluid analysis to obtain tsunami-induced wave impact force. Furthermore, a fiber model, which is commonly used in the seismic analysis of steel bridge structures, was adopted considering material and geometric nonlinearity. The residual stresses induced by the earthquake were applied into the numerical model during the following finite element analysis as the initial stress state, in which the acquired tsunami forces were input to a whole bridge system. Based on the analytical results, it can be seen that the foundation sliding was not observed although the caisson foundation came floating slightly, and the damage arising during the earthquake did not expand when the tsunami-induced wave impact is applied to the steel bridge. It is concluded that the influence of tsunami-induced wave force is relatively small for such steel bridge with large caisson foundations. Besides, a numerical procedure is proposed for quantitatively estimating the accumulative damage induced by the earthquake and the tsunami in the whole bridge system with large caisson foundations.