• Journal of Microbiology
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• 제43권6호
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• pp.516-522
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• 2005

The phosphorylation of C-terminal domain (CTD) of Rpb1p, the largest subunit of RNA polymerase II plays an important role in transcription and the coupling of various cellular events to transcription. In this study, its role in DNA damage response is closely examined in Saccharomyces cerevisiae, focusing specifically on several transcription factors that mediate or respond to the phosphorylation of the CTD. CTDK-1, the pol II CTD kinase, FCP1, the CTD phosphatase, ESS1, the CTD phosphorylation dependent cis-trans isomerase, and RSP5, the phosphorylation dependent pol II ubiquitinating enzyme, were chosen for the study. We determined that the CTD phosphorylation of CTD, which occurred predominantly at serine 2 within a heptapeptide repeat, was enhanced in response to a variety of sources of DNA damage. This modification was shown to be mediated by CTDK-1. Although mutations in ESS1 or FCP1 caused cells to become quite sensitive to DNA damage, the characteristic pattern of CTD phosphorylation remained unaltered, thereby implying that ESS1 and FCP1 play roles downstream of CTD phosphorylation in response to DNA damage. Our data suggest that the location or extent of CTD phosphorylation might be altered in response to DNA damage, and that the modified CTD, ESS1, and FCP1 all contribute to cellular survival in such conditions.

• 한국지진공학회:학술대회논문집
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• 한국지진공학회 2005년도 학술발표회 논문집
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• pp.90-101
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• 2005

For the site characterization at two representative inland areas, Gyeongju and Hongsung, in Korea, in-situ seismic tests containing boring investigations and resonant column tests were performed and site-specific ground response analyses were conducted using equivalent linear as well as nonlinear scheme. The soil deposits in Korea were shallower and stiffer than those in the western US, from which the site classification system and site coefficients in Korea were derived. Most sites were categorized as site classes C and D based on the mean shear wave velocity to 30 m, Vs30 ranging between 250 and 650 m/s. Based on the acceleration response spectra determined from the site-specific analyses, the site coefficients specified in the Korean seismic design guide underestimate the ground motion in the short-period band and overestimate the ground motion in mid-period band. These differences can be explained by the differences in the bedrock depth and the soil stiffness profile between Korea and western US. The site coefficients were re-evaluated and the preliminary site classification system was introduced accounting for the local geologic conditions on the Korean peninsula.

• 대한의생명과학회지
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• 제16권1호
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• pp.65-67
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• 2010

The endoplasmic reticulum (ER) is a multifunctional intercellular organelle in which several posttranslational modification steps occurred such as protein folding, lipid biosynthesis, calcium storage and release. Perturbations that disrupt ER homeostasis lead to the misfolding of proteins in the ER lumen and up-regulation of ER signaling pathway called the unfolded protein response (UPR). Here, we have demonstrated that ageing changes the expression of ER chaperone and associated ER membrane kinases of IRE1, ATF6 and PERK.

• 한국소음진동공학회:학술대회논문집
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• 한국소음진동공학회 2002년도 추계학술대회논문집
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• pp.301-305
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• 2002

Among many structural dynamics modification methods for plate and shell vibration problems, embedding an emboss to the surface is very efficient. But deciding an optimal position and shape using optimization algorithm needs defining geometry and remeshing the model for every iteration step to implement the method, which takes much numerical cost. An equivalent element produced here lessen the cost by representing the geometrical characteristics of an emboss using the element's material properties and thickness becoming a geometrically homogenous element of the base plate or shell. Some efficient factors which let the equivalent system have the same dynamical response as the original system embedded with emboss will be shown and the degree of equivalence will be tested in terms of natural frequency matching.

• 한국자동차공학회논문집
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• 제14권3호
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• pp.164-169
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• 2006

A systematic methodology has been proposed to establish a reliable design of water pump system. A simplified steady-state dynamic model of water pump system has been developed to study the response of water pump system to the dynamic load mainly due to the run-out and unbalance. Design modifications are needed to strengthen the structural integrity of existing designs. Increasing the natural frequency of system is pursued to prevent a resonance from occurring in the engine excitation range. A computational reliability methodology combined with finite element analysis is used to identify the most significant factor affecting the system performance. This method considered influence of design control parameters for the performance of design. By including control factors to the system model in a systematic way, more reliable design is expected.

• Computers and Concrete
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• 제1권1호
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• pp.77-98
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• 2004

This paper presents a numerical model for simulating the nonlinear response of reinforced concrete (RC) shear walls subject to cyclic loadings. The material behavior of cracked concrete is described by an orthotropic constitutive relation with tension-stiffening and compression softening effects defining equivalent uniaxial stress-strain relation in the axes of orthotropy. Especially in making analytical predictions for inelastic behaviors of RC walls under reversed cyclic loading, some influencing factors inducing the material nonlinearities have been considered. A simple hysteretic stress-strain relation of concrete, which crosses the tension-compression region, is defined. Modification of the hysteretic stress-strain relation of steel is also introduced to reflect a pinching effect depending on the shear span ratio and to represent an average stress distribution in a cracked RC element, respectively. To assess the applicability of the constitutive model for RC element, analytical results are compared with idealized shear panel and shear wall test results under monotonic and cyclic shear loadings.

• Earthquakes and Structures
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• 제2권2호
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• pp.127-147
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• 2011

In a companion paper as well as in earlier publications, it has been shown that in asymmetric frame buildings, designed in accordance with modern codes and subjected to strong earthquake excitations, the ductility demands at the so called "flexible" edges are consistently and substantially higher than the ductility demands at the "stiff" edges of the building. In some cases the differences in the computed ductility factors between elements at the two opposite building edges exceeded 100%. Similar findings have also been reported for code designed reinforced concrete buildings. This is an undesirable behavior as it indicates no good use of material and the possibility for overload of the "flexible" edge members with a consequent potential for premature failure. In the present paper, a design modification will be introduced that can alleviate the problem and lead to a more uniform distribution of ductility demands in the elements of all building edges. The presented results are based on the steel frames detailed in the companion paper. This investigation is another step towards more rational design of non-symmetric steel buildings.

• 한국학교보건학회지
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• 제17권1호
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• pp.55-69
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• 2004

Purpose ： To describe epidemiological characteristics and related factors in school personnel with hypertension. Method ： 5,384 school personnel (2,638 males and 2,746 females) received physical examination at the Seoul School Health Center in 2002. blood pressure(BP) was classified by JNC 7th report. Normal BP is systolic BP <120 and diastolic BP<80, prehypertension BP is 120-139 or 80-89mmHg, stage 1 hypertension BP is 140-159 or 90-99, and stage 2 hypertension >=160 or >=100 mmHg. each group was evaluated by gender. We used multiple regression and logistic regression. Results ： Normotension was 20.13% in males and 48.65% in female, prehypertension 43.4% in males and 38.06% in females, stage 1 hypertension 26.38% in males and 10.99% in females, stage2 hypertension 9.59% in males and 3.2% in females. the older age group had higher distribution of stage 1 hypertension and stage 2 hypertension. The means and abnormal rates of BMI, blood sugar, total cholesterol, GOT, GPT, and GTP was higher when their blood pressure was higher. However, an alcohol habit among lifestyle factors had an inverse effect. Higher Bp was correlated to a higher BMI, FBS, and cholesterol-like dose response. In stage 1 hypertension, the related factors of hypertension for males were BMI, amount of cigarettes smoked, exercise, blood sugar, total cholesterol, and GTP. In stage 1 hypertension, age, BMI, exercise, blood sugar, total cholesterol were related factors in female. In stage 2 hypertension, age and smoking were related factors in male, and age, BMI, and cholesterol in female. Conclusions ： It is possible to intervene in all related factors of hypertension except age through life-style modification and appropriate medical management. Active health promotion is needed in School personnel.

• 한국발생생물학회지:발생과생식
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• 제24권1호
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• pp.1-17
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• 2020

Phthalates and those metabolites have long history in industry and suspected to have deficient effects in development and reproduction. These are well-known anti-androgenic chemicals and many studies have examined the effects of these compounds on male reproduction as toxins and endocrine disruptors. Uterus is a key organ for proper embryo development, successful reproduction, and health of eutherian mammals including women. To understand the effects of the phthalate, the horizontal approach with a whole group of phthalate is best but the known phthalates are huge and all is not uncovered. Di-(2-ethylhexyl) phthalate (DEHP) is the most common product of plasticizers in polymer products and studied many groups. Although, there is limited studies on the effects of phthalates on the female, a few studies have proved the endocrine disrupting characters of DEHP or phthalate mixture in female. An acute and high dose of DEHP has adverse effects on uterine histological characters. Recently, it has been revealed that a chronical low-dose exposing of DEHP works as endocrine disrupting chemicals (EDC). DEHP can induce various cellular responses including the expression regulation of steroid hormone receptors, transcription factors, and paracrine factors. Interestingly, the response of uterus to DEHP is not monotonous and the exposed female has various phenotypes in fertility. These suggest that the exposing of DEHP may causes of histological modification in uterus and of disease in female such as endometriosis, hyperplasia, and myoma in addition to developmental and reproductive toxicity.

• Earthquakes and Structures
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• 제19권3호
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• pp.197-214
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• 2020

Diagrid structures have been introduced as a fairly modern lateral load-resisting system in the design of high-rise buildings. In this paper, a novel diagrid system called tube-in-tube diagrid building is introduced and assessed through pushover and incremental dynamic analyses. The main objectives of this paper are to find the optimum angle of interior and exterior diagrid tube and evaluate the efficiency of diagrid core on the probability of collapse comparing to the conventional diagrid system. Finally, the seismic performance factors of the proposed system are validated according to the FEMA P695 methodology. To achieve these, 36-story diagrid buildings with various external and internal diagonal angles are designed and then 3-D nonlinear models of these structures developed in PERFORM-3D. The results show that weight of steel material highly depends on diagonal angle of exterior tube. Adding diagrid core generally increases the over-strength factor and collapse margin ratio of tall diagrid buildings confirming high seismic safety margin for tube-in-tube diagrid buildings under severe excitations. Collapse probabilities of both structural systems under MCE records are less than 10%. Finally, response modification factor of 3.0 and over-strength factor of 2.0 and 2.5 are proposed for design of typical diagrid and tube-in-tube diagrid buildings, respectively.