• Animal Bioscience
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• 제37권6호
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• pp.1021-1030
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• 2024

Objective: R-loops are DNA:RNA triplex hybrids, and their metabolism is tightly regulated by transcriptional regulation, DNA damage response, and chromatin structure dynamics. R-loop homeostasis is dynamically regulated and closely associated with gene transcription in mouse zygotes. However, the factors responsible for regulating these dynamic changes in the R-loops of fertilized mouse eggs have not yet been investigated. This study examined the functions of candidate factors that interact with R-loops during zygotic gene activation. Methods: In this study, we used publicly available next-generation sequencing datasets, including low-input ribosome profiling analysis and polymerase II chromatin immunoprecipitation-sequencing (ChIP-seq), to identify potential regulators of R-loop dynamics in zygotes. These datasets were downloaded, reanalyzed, and compared with mass spectrometry data to identify candidate factors involved in regulating R-loop dynamics. To validate the functions of these candidate factors, we treated mouse zygotes with chemical inhibitors using in vitro fertilization. Immunofluorescence with an anti-R-loop antibody was then performed to quantify changes in R-loop metabolism. Results: We identified DEAD-box-5 (DDX5) and histone deacetylase-2 (HDAC2) as candidates that potentially regulate R-loop metabolism in oocytes, zygotes and two-cell embryos based on change of their gene translation. Our analysis revealed that the DDX5 inhibition of activity led to decreased R-loop accumulation in pronuclei, indicating its involvement in regulating R-loop dynamics. However, the inhibition of histone deacetylase-2 activity did not significantly affect R-loop levels in pronuclei. Conclusion: These findings suggest that dynamic changes in R-loops during mouse zygote development are likely regulated by RNA helicases, particularly DDX5, in conjunction with transcriptional processes. Our study provides compelling evidence for the involvement of these factors in regulating R-loop dynamics during early embryonic development.

• 생물정신의학
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• 제6권2호
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• pp.176-188
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• 1999

Objective : The purpose of this study was to evaluate the effects of alcohol on neurocognitive function, psychomotor performance and subjective response in healthy Korean adults with different ALDH2 genotypes. Method : A total of 24 males, half with active $ALDH2^*1/2^*1$ and the other with inactive $ALDH2^*1/2^*2$, was selected through genotyping using restriction fragment length polymorphism. In a double-blind, placebo-controlled cross-over design, each subject consumed 0.5g/kg dose of alcohol, given as a mixture of 40% vodka and orange juice, and placebo(orange juice) on two separate occasions on an average of weekly intervals. The blood alcohol concentrations(BACs) were measured using a breath analyzer at baseline and at 30, 60 minutes after drinking. P300s were measured at baseline and at 30 minutes after alcohol and placebo intake. Vital signs and psychomotor performance[Critical Flicker Fusion Threshold(CFFT), Choice Reaction Time (CRT), Digit Symbol Substitution(DSS)] were measured at baseline and at 60 minutes after alcohol and placebo intake. Subjective responses were measured at the end of the study. The statistical analysis focused on whether there were any differences between groups with different ALDH2 genotypes. Results : The major results are as follows. 1) BACs in the inactive group were overall equivalent to those in the active group. Only in terms of time, BACs were significantly higher overall at 30 minutes than at 60 minutes after alcohol intake. 2) Pulse rates were significantly increased after alcohol intake compared with placebo, and the increase was greater in the inactive than in the active group. 3) P300 latencies in leads Fz(frontal), Cz(cental) and Pz(parietal) were significantly increased after alcohol intake compared to placebo, and the increase was greater in the inactive than in the active group. P300 amplitudes in leads Cz and Pz were significantly decreased overall after alcohol intake compared to placebo. 4) Compared with placebo, alcohol produced significant effect on the psychomotor performance : impairment in the inactive group, improvement in the active group. 5) Compared with placebo, alcohol significantly induced a negative or an intense effect on the subjective responses in the inactive group, but little negative and even a somewhat positive effect in the active group. Conclusions : These results suggest that ALDH isozyme variance might be an important factor to determine the effects of acute dose of alcohol on the various psychobehavioural functions and also to determine the alcohol use pattern and to predict the future development of alcohol overuse and/or abuse.

• 동의신경정신과학회지
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• 제20권2호
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• pp.85-99
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• 2009

Objectives : This study aimed to understand the influence of acupuncture on the human body by comparing changes within human bodies before and after people in normal health are treated with acupuncture at the acupoints HT7 and PC9, which are related to mental functions. Methods : The study was performed from January 3, 2008 to March 5, 2008 on 60 healthy males and females in their 20s. HRV, EEG, skin conductance response, respiration and peripheral skin temperature were measured for 5 minutes before acupuncture simulation was applied to the acupoints HT7 and PC9. During 20 minutes of acupuncture treatment, the same items were continuously measured to determine whether there had been any changes, and they were then measured for 5 minutes after the removal of the acupuncture needles in order to implement a comparative analysis. Results : 1. The HRV measurement showed that in the course of before, during and after acupuncture stimulation, heart rate, HF and HF norm decreased significantly (P<0.05) at HT7. LF, LF norm, and LF/HF ratio increased significantly (P>0.05), while heart rate, HF and HF norm decreased significantly (P<0.05) at PC9. 2. Skin conductance response increased significantly (P<0.05) at PC9 during and after the acupuncture simulation periods, compared with the pre acupuncture period. 3. the peripheral skin temperature increased significantly (P<0.05) both at HT7 and PC9 in the course of before, during and after acupuncture stimulation. 4. Compared with the pre-acupuncture period, respiration rate increased both at HT7 and at PC9 during and after the acupuncture simulation periods, but not in a statistically significance. 5. In the EEG measurement, when compared with the pre-acupuncture period at HT7, mid ${\beta}$ wave decreased significantly (P<0.05) during acupuncture treatment. Compared with the measurements during acupuncture treatment at PC9, low ${\beta}$ wave increased significantly (P<0.05) after the acupuncture needles were removed. Conclusions : When acupuncture treatment is applied at the acupoints HT7 and PC9, the activation of parasympathetic nerves decreases and the activation of sympathetic nerves increases in the HRV measurement. It was determined that PC9 makes the sympathetic nerves become highly activated in a skin conduction response. The effect of stability in the brain wave seemed to bo shown at HT7 than PC9.

• 한국지진공학회논문집
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• 제10권3호
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• pp.85-100
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• 2006

이 논문에서는 서로 다른층 높이를 갖는 인접한 두 빌딩의 내진성능을 효율적으로 향상시킬 수 있는 선형 점성 감쇠기의 최적 설계방법을 제시하고자 한다. 이를 위하여 층간 대각 브레이싱 형태의 에너지 소산장치 연결방법과 인접 구조물간 에너지 소산장치 연결방법을 고려하였으며, 두 가지 연결방법을 적용한 시스템에 대한 감쇠용량별 주파수응답함수 비교를 통하여 구조물간 연결방법의 효율성을 확인하였다. 아울러 구조물간 연결방법에서 주파수응답함수를 최소화하는 최적 감쇠용량이 존재하는 것을 보이고, 최적 설계된 시스템에 대하여 감쇠용량별 구조물의 고유주파수 및 등가감쇠비의 민감도를 분석하였다. 민감도 분석 결과로부터 고층부에 설치되는 에너지 소산장치가 구조물의 등가 감쇠비를 효율적으로 증가시키는 것을 확인하였다. 따라서 민감도에 비례하는 새로운 감쇠기 설계방법을 제시하고, 대각 브레이싱 연결방법과 구조물간 연결로서 균등분포 및 제시하는 민감도 기반 분포에 따른 연결방법을 적용한 시스템들의 내진성능을 비교 분석하였다. 지진응답의 비교결과, 제시하는 방법이 인접 구조물의 효과적인 내진설계방법이 될 수 있음을 입증하였다.

• 한국항만경제학회지
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• 제28권3호
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• pp.111-125
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• 2012

광양항의 컨테이너 물동량은 인천항이나 평택 당진항에 비해 더딘 증가를 보이고 있으며, 부단위당 부가가치가 낮고 중국시장을 제대로 활용하지 못하고 있다. 광양항은 다른 두 항에 비해 환율계수와 경기계수가 크게 낮아 환율변동과 경기변동에 가장 작은 영향을 받게 되고 그에 따라 경기와 환율의 긍정적 변동을 이용하는데 가장 서투른 항만임을 보인다. 오차수정방정식을 도출하여 오차수정계수가 광양항에서 가장 작아서 적정 물동량 수준에 이르지 못할 경우 물동량 수준으로 돌아가는데 다른 두 항만에 비해 크게 떨어진다는 것을 밝힌다. 이것은 광양항이 다른 두 항만에 비해 물동량 확보능력이 부족하다는 것과, 물동량 부족이 발생할 경우 그것이 오래 지속될 수 있다는 것을 의미한다. 충격반응함수를 이용하여 경기충격이 광양항에 대해 가장 낮은 반응을 야기하여 경기상승에 따른 물동량 증가가 가장 적을 것이라는 것을 밝힌다. 마지막으로 2012년과 2013년의 물동량을 개입-승법계절 ARIMA 모형을 통해 예측하여 광양항은 2012년과 2012년 전년 대비 2.6%, 3.1% 증가하는데 비해, 인천항 6.8%, 8.1%, 평택 당진항 24.2%, 10.3%가 증가하여, 2012년에는 인천항과 물동량 차이가 거의 없는 수준으로 좁혀지고 2013년에는 비교적 큰 차이로 인천항이 광양항을 앞설 것이라는 것을 보인다. 경제적 또는 경제외적 요인의 변화에 대한 광양항의 적응능력이 타 항만에 비해 떨어지고, 그에 따라 다른 항만에 추월당할 상태에 처하고 있다는 것을 밝힌다.

• Asian Pacific Journal of Cancer Prevention
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• 제15권11호
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• pp.4499-4505
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• 2014

Glioblastoma, the most aggressive and malignant form of glioma, appears to be resistant to various chemotherapeutic agents. Hence, approaches have been intensively investigated to targeti specific molecular pathways involved in glioblastoma development and progression. Aloe emodin is believed to modulate the expression of several genes in cancer cells. We aimed to understand the molecular mechanisms underlying the therapeutic effect of Aloe emodin on gene expression profiles in the human U87 glioblastoma cell line utilizing microarray technology. The gene expression analysis revealed that a total of 8,226 gene alterations out of 28,869 genes were detected after treatment with $58.6{\mu}g/ml$ for 24 hours. Out of this total, 34 genes demonstrated statistically significant change (p<0.05) ranging from 1.07 to 1.87 fold. The results revealed that 22 genes were up-regulated and 12 genes were down-regulated in response to Aloe emodin treatment. These genes were then grouped into several clusters based on their biological functions, revealing induction of expression of genes involved in apoptosis (programmed cell death) and tissue remodelling in U87 cells (p<0.01). Several genes with significant changes of the expression level e.g. SHARPIN, BCAP31, FIS1, RAC1 and TGM2 from the apoptotic cluster were confirmed by quantitative real-time PCR (qRT-PCR). These results could serve as guidance for further studies in order to discover molecular targets for the cancer therapy based on Aloe emodin treatment.

• 보건행정학회지
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• 제4권2호
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• pp.27-57
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• 1994

The objective of running a long-term care institution is to provide services helpful for maintaining, supporting, and improving elderlies' optimum level of physical, mental, and psychosocial functioning. For the purpose of analyzing the current situations of institutional long term care facilities in Korea, 27 facilities were selected proportionately from each of the cities and provinces, out of the total 152 facilities. About 20% of those who were institutionalized during 25 August through 2 Qctober 1993, the 391 elderlies were chosen on a systematic random basis. The instrument of this study was developed by modifying the tools of CARE, MAI and PCTC. A multivariate approach of discriminant analysis and clustering technique were employed for this study. The Stiudy reveals that there is no clear differentiation of goals and functions among the longterm care institutions in Korea. Staffing patte군 of long-term care facilities shows a shortage of nurses, physical therapists, and dieticians. The linkage between acute care facilities and long-term care is weak, and administration of long-term care faciltiy is carried out by non-professionals. They are responsible for assessing health status before entering the facility, and evaluating elderlies' care. Therefore, it is not surprising to find that most of the facilities have accommodated agede regardless of their real needs and health status. Based upon findings of the analysis, this study has classified long-term care facilities into four types : Type I is to help elderlies maintain independence in daily living activities. Type II facilities have the objective of maintaining and improving the current level of elderlies' function. Type III is to maintain maximum independence of elderlies in activities of daily living. And Type IV is identified for the group of facilities designed to restore or improve functional abilities of elderlies. In conclusion, the following suggestions are made : the need for long-term care should be assessed by multidimensional measurement. Institutional long-term care facilities should be classified and developed in response to type of type of care and service need. Both acute and long-term care facilities should be linked together in order to support the evaluation of service operation and program development.

• 정보통신설비학회논문지
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• 제2권1호
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• pp.52-62
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• 2003

본 논문에서는 그라운드 판에 주기적으로 에칭된 원으로 구성된 Photonic Bandgap(PBG) 여파기가 광대역 하모닉 성분을 제거할 수 있음을 보인다. FDTD법과 제작, 실험을 통해 얻은 결과를 이용하여 PBG 구조의 특성을 분석해 보았고, FDTD법은 다양한 3차원 구조를 시뮬레이션을 할 수 있고 광대역의 주파수 응답을 볼 수 있다. 분석 결과는 에칭된 원들을 2차원으로 배열한 경우와 일렬로 배열한 경우 모두 똑같은 결과가 나오는 것을 나타냈다. 그리고 에칭된 원과 원 사이의 공진기의 특성을 필드패턴을 통해서 알아 보았고, 에칭된 원들의 수 n, 에칭된 원의 반지름 r과 주기 a의 변화에 따른 주파수의 특성을 보았다.

• BMB Reports
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• 제39권1호
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• pp.26-36
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• 2006

Differential Display PCR technique (DD-PCR) was used for the analysis of altered gene expression in hemocytes of Vibrio harveyi-infected Penaeus monodon. Forty-four combinations of arbitrary and oligo(dT) primers were used to screen for differentially expressed genes. A total of 79 differentially expressed bands could be identified from 33 primer combinations. These included 48 bands (61%) whose expression level increased and 31 bands (39%) decreased after V. harveyi challenge. Subsequently, forty-eight differential display fragments were successfully reamplified and cloned. A total of 267 clones were randomly selected and sequenced. The sequence analysis showed that 85 (31%) out of 267 clones were matched with sequences in the GenBank database which represented 24 different genes with known functions. Among the known genes, glucose transporter 1, interferon-related developmental regulator 1, lysozyme, profilin, SERPINB3, were selected for further confirmation of their differentially expression patterns by real-time PCR. The results showed increasing in expression level of the selected genes in shrimp hemocytes after microbial challenge suggesting the involvement of such genes in bacterial response in shrimp. The anti-lipopolysaccharide factor type 3 (ALFPm3) gene, previously reported in P. monodon (Supungul et al., 2002) was found among the up-regulated genes but diversity due to amino acid changes was observed. Increase in ALFPm3 transcripts upon V. harveyi injection is in accordance with that found in the previous study.

• 한국소음진동공학회논문집
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• 제22권12호
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• pp.1243-1249
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• 2012

이 논문에서 유한요소 모형은 2단계로 개선된다. 첫 단계에서는 감쇠를 무시하고, 최적화 방법을 사용하여 유한요소 모형의 질량행렬과 강성행렬을 개선한다. 최적화를 위한 목적함수는 모드시험 데이터와 유한요소해석으로부터 구한 고유진동수와 진동형으로 이루어져 있다. 두 번째 단계에서는 첫 단계에서 구한 질량행렬과 강성행렬을 고정시키고, 감쇠를 고려한다. 먼저 비례감쇠를 가정하고 감쇠행렬을 추정한 다음, 해석적인 주파수응답함수와 측정한 주파수응답함수의 차가 최소가 되도록 최적화 과정을 이용하여 감쇠행렬을 조정한다. 이와 같은 모형개선 방법을 시뮬레이션 계와 실제 외팔보에 적용하였다.