• 제목/요약/키워드: resonance energy

검색결과 880건 처리시간 0.026초

Backbone NMR chemical shift assignment for the substrate binding domain of Escherichia coli HscA

  • Jin Hae Kim
    • 한국자기공명학회논문지
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    • 제28권2호
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    • pp.6-9
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    • 2024
  • HscA is a Hsp70-type chaperone protein that plays an essential role to mediate the iron-sulfur (Fe-S) cluster biogenesis mechanism in Escherichia coli. Like other Hsp70 chaperones, HscA is composed of two domains: the nucleotide binding domain (NBD), which can hydrolyze ATP and use its chemical energy to facilitate the Fe-S cluster transfer process, and the substrate binding domain (SBD), which directly interacts with the substrate, IscU, the scaffold protein of an Fe-S cluster. In the present work, we prepared the isolated SBD construct of HscA (HscA(SBD)) and conducted the solution-state nuclear magnetic resonance (NMR) experiments to have its backbone chemical shift assignment information. Due to low spectral quality of HscA(SBD), we obtained all the NMR data from the sample containing the peptide LPPVKIHC, the HscA-interaction motif of IscU, from which the chemical shift assignment could be done successfully. We expect that this information provides an important basis to execute detailed structural characterization of HscA and appreciate its interaction with IscU.

SEPARATION OF GAMMA-RAYS PRODUCTION FROM $^{13}C(p,\;{\gamma})^{14}N,\;^{14}N({\gamma},\;{\gamma})^{14}N$ REACTIONS USING DOPPLER SHIFT EFFECT

  • Kim, Y.K.;Ha, J.H.;Youn, M.;Han, S.H.;Chung, C.E.;Moon, B.S.
    • Journal of Radiation Protection and Research
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    • 제26권3호
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    • pp.287-290
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    • 2001
  • The 9.17MeV gamma-rays from the $^{13}C(p,\;{\gamma})^{14}N,\;^{14}N({\gamma},\;{\gamma})^{14}N$ reactions were measured. The incident 9.17MeV gamma-ray was produced from the $^{13}C(p,\;{\gamma})^{14}N$ reaction at Ep=1.75MeV resonance. The 1.75MeV proton beam was accelerated using the 3MV SNU-AMS Tandetron and 1.7MV KIGAM Tandem accelerators. The enriched 13C target was $121{\mu}g/cm^2$ self-supporting foil, and we used liquid nitrogen as a resonant absorption target. We used a HP-Ge detector with 30% efficiency and less 2keV energy resolution. We developed new method to detect the scattered 9.17MeV gamma-ray from the nitrogen target by using the energy difference between the Doppler shifted gamma-ray from the $^{13}C(p,\;{\gamma})^{14}N$ reaction and the resonant absorbed and rescattered gamma-ray from the $^{14}N({\gamma},\;{\gamma})^{14}N$ reaction.

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ANALYSIS ON THE VIBRO-ACOUSTICAL CHARACTERISTICS OF A PANEL-CAVITY COUPLED SYSTEM

  • Kim, Seock-Hyun;Kang, Sang-Wook;Lee, Jang-Moo
    • Journal of Theoretical and Applied Mechanics
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    • 제3권1호
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    • pp.34-44
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    • 2002
  • Theoretical analysis Is carried out to identify the modal coupling effect between some particular acoustic modes of a vehicle compartment cavity and vibration modes of body panels like side doors, roof or floor. A simplified panel-cavity coupled model is investigated on the coupled resonance frequencies, modes and frequency response characteristics. Through parametric study, It Is possible to explain how the acoustic response of a coupled system will be determined by the vibration and acoustic property of the individual panel and cavity system. Full coupled system shows some interesting features different from those of the semi-coupled system In frequency, mode and acoustic response.

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Solution Structure of Bovine Pancreatic Trypsin Inhibitor using NMR Chemical Shift Restraints

  • Park, Kyunglae;Wil
    • 한국자기공명학회논문지
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    • 제1권2호
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    • pp.79-94
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    • 1997
  • The solution structure of bovine pancreatic trypsin inhibitor(BPTI) has been refined by NMR chemical shift data of C${\alpha}$H using classical molecular dynamics simulation. The structure dependent part of the observable chemical shift was modeled by ring current effect, magnetic anisotropy effect from the nearby groups, whereas the structure independent part was replaced with the random coil shift. A new harmonic function derived from the differences between the observed and calculated chemical shifts was added into physical force field as an pseudo potential energy term with force constant of 250 kJmol-1 ppm-2. During the 1.5 ns molecular dynamics simulation with chemical shift restraints BPTI has accessed different conformation space compared to crystal and NOE driven structure.

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양단 공진과 듀티 컨트롤을 이용한 1-2cm 공극을 통한 에너지 전달 (Transcutaneous Energy Transmission with Double Tuned Duty Cycle Control)

  • 김창균;조보형
    • 대한전기학회:학술대회논문집
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    • 대한전기학회 1996년도 하계학술대회 논문집 A
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    • pp.486-488
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    • 1996
  • A dc-dc converter which transfers l2-48W of power(regulated output voltage 24V) across a large, variable air gap(1-2cm) has been designed. This converter employs double resonance to compensate the large leakage inductance of the primary and secondary of the transcutaneous transformer. To maximize the effect of resonance, a constant frequency, duty cycle control method is used. The duty cycle control presents advantages over a frequency control in terms of increase in dc voltage gain and reduction of the primary current. The analysis and design procedure for the proposed scheme is presented and the performance result is simulated and verified experimentally.

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광픽업 잔류 진동 저감을 위한 이송 속도 분포 해석 (Velocity Profile Analysis to Reduce Residual Vibration in Optical Pick-up)

  • 전홍걸;박영필
    • 소음진동
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    • 제10권2호
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    • pp.221-228
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    • 2000
  • In this paper, analytical study is performed to reduce residual vibration in seeking mode of optical pick-up. The conditions for acceleration adn deceleration time in trapezoidal velocity profile to reduce residual vibration are derived for undamped vibration system. To verify the validity of conditiosn two example studies are carried out. Numerical and experimental implementations for flexible arm system attached to moving part show that residual vibration is effectively reduced by calculated velocity profile. In addition, simulation study for optical pick-up reveals that by changing natural frequency to resonance frequency the conditions derived assuming undamped system can be applied to obtain velocity profile for minimum residual energy in damped vibration system.

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이종재료 접합을 위한 초음파 진동자 설계 (Design Method for Ultrasonic Transducer to Bonding with Dissimilar Materials)

  • 정안목;김철호
    • 한국소음진동공학회논문집
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    • 제22권1호
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    • pp.3-8
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    • 2012
  • In an attempt to improve adhesion strength between glass and metal due to use of Pb-free solder as a sealant between glass and metal in the manufacturing process of vacuum insulation window glass to maintain the vacuum volume, ultrasonic energy is often applied during the process of Pb-free sealing. In this study, we propose an ultrasonic vibrator with a 4 mm end tip radius which performs resonance frequency of 60 kHz and 14 um or higher vibration displacement. A frequency variation due to applied pressure on piezo disks, which was excluded in the computer simulation, was verified experimentally, and we have demonstrated a 17 um vibration displacement at 50 V input through the performance test of a vibrator constructed with our specification.

허니콤 구조물을 갖는 차체 바닥의 투과손실 해석기법 연구 (Research on the Transmission Loss Analysis of the Honeycomb Structure's Floor Section)

  • 김관주;김상수;박진규
    • 한국철도학회논문집
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    • 제11권1호
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    • pp.1-6
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    • 2008
  • 본 연구에서는 현 고속 차량의 차체바닥 구조의 투과손실 특성 파악을 연구하고자 하였다. 이를 위하여 우선 허니콤 구조인 알루미늄 시편을 제작하여 투과손실 측정 실험을 수행하였다. 측정된 결과에서는 800Hz이상부터 질량 법칙을 따르는 투과손실의 특성을 파악할 수 있었다. 상용프로그램인 NASTRAN, AUTOSEA2를 사용하여 구조물에 대한 투과손실을 해석한 후 이를 실험치와 비교하여 신빙성 있는 결과를 얻을 수 있었다. 아울러 다른 단면 형상을 갖는 허니콤 구조물에 대한 투과손실 해석을 수행하여 특성을 예측해 보았다.

Low-Cost High-Efficiency PDP Sustaining Driver with a Resonance Bias Level Shift

  • Park, Kyung-Hwa;Yi, Kang-Hyun
    • Journal of Power Electronics
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    • 제13권5호
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    • pp.779-786
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    • 2013
  • A highly efficient sustaining driver is proposed for plasma display panels (PDPs). When the PDP is charged and discharged, the proposed sustaining driver employs an address voltage source used in an addressing period. A voltage source is used for fully charging the panel to the sustaining voltage, and an initial inductor current helps the panel discharge to 0 V. The resonance between the panel and an inductor is made by shifting the voltage and current bias level when charging and discharging the panel. As a result, the proposed circuit can reduce power consumption, switching loss, heat dissipation, and production cost. Experimental results of a 42-inch PDP are provided to verify the operation and features of the proposed circuit.

Prediction of Resonance Frequency rind Mode Shape of Rotor in Switched Reluctance Motor

  • Ha, Kyung-Ho;Jeong, Seung-Kyu;Hong, Jung-Pyo;Kang, Do-Hyun
    • KIEE International Transaction on Electrical Machinery and Energy Conversion Systems
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    • 제12B권1호
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    • pp.19-23
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    • 2002
  • This paper investigates the influence of various stator pole shapes and yoke structures in Switched Reluctance Motors (SRM) on the mechanical behavior caused by the electromagnetic farce. The stator part in SRM produces most vibration. The geometric design of the stator is therefore necessary to reduce the vibration. Based on electromagnetic and structural Finite Element Method (FEM), the free and farced vibrations for the various structures of SRM with 6/4 poles are analyzed. Then a less vibration stator structure is proposed. Some of numerical computations for a prototype motor are verified by experimental results.