• 제목/요약/키워드: residue study

검색결과 1,374건 처리시간 0.026초

Application of ELISA for the Detection of Sulfamethazine Residue in Live Cattle

  • Lee, H.J.;Lee, M.H.;Han, In K.
    • Asian-Australasian Journal of Animal Sciences
    • /
    • 제14권3호
    • /
    • pp.378-381
    • /
    • 2001
  • Sulfamethazine has been widely used in swine for prevention or treatment of infections. Recently, the safety of the drug to consumers has been questioned because of carcinogenic effects. To prevent unwanted drug residues entering the human food chain, both government authorities and industries have established extensive control measures. The demands for reliable, simple, sensitive, rapid and low-cost methods for residue analysis of foods are increasing nowadays. In this study, we established a rapid prediction test for the detection of cattle with violative tissue residues of sulfamethazine. The recommended therapeutic dose of sulfamethazine (withdrawal time, 15 days) was administered to each of 10 cattle. Blood was sampled before drug administration and during the withdrawal period. The concentration of sulfamethazine in plasma, determined by a semi-quantitative ELISA, was compared to that of an internal standard (10 ppb). The absorbance ratio of internal standard to sample (B/Bs) was employed as an index to determine whether drug residues in cattle tissues were negative or positive. That is, a B/Bs ratio less than 1 was considered residue positive and if larger than 1 was considered negative. All 10 plasma samples from non-treated cattle showed negative to sulfamethazine. Sulfamethazine was detected in plasmas of treated cattle until Day 7 of withdrawal period. The present study showed that the semi-quantitative ELISA could be easily adapted in predicting residues of sulfamethazine in live cattle.

Antimicrobial Effect of Natural Medicines on Bacterial Species from Soybean Curd Residue

  • Kim, Seong-Sun;Jin, Yu-Mi;Jeon, Yong-Deok;Jin, Jong-Sik
    • 한국자원식물학회:학술대회논문집
    • /
    • 한국자원식물학회 2019년도 추계학술대회
    • /
    • pp.102-102
    • /
    • 2019
  • Soybean curd residue (SCR), known as a major waste product of soybean processing, is the water-insoluble fraction which is removed by filtration during soymilk production. For these reasons, SCR was usually considered as food waste. SCR might have a good potential as a functional food material, value-added processing and utilization. SCR contains high-quality protein and consists of a good source of nutrients, including protein, oil, dietary fiber, minerals, along with un-specified monosaccharides and oligosaccharides. Also, SCR might be a potential source of low cost protein for human consumption. However, SCR could be a source of bacterial contamination when during food processing. This study was aimed to investigate antibacterial capacity of natural product through detecting relationship between SCR and microbial. We isolated five bacterial strains from SCR and elucidated antibacterial activity of nature medicines to extend storage capacity of food made with SCR. Thus, the extract which showed antibacterial effects in Corynebacterium calloonae and Raoultella amithinolytica is a combination of seven kinds of extracts: Glycyrrhiza uralensis, Cudrania tricuspidata, Salvia miltiorrhiza Bunge, blueberry, Acorus gramineus, Ginkgo biloba L., Camellia sinensis. This study suggested that antibacterial activities of natural medicines could be used for extension of storage capacity in SCR-contained food.

  • PDF

물오리나무 유래 고함량 Oregonin 추출방법 및 생리활성 연구 (Extraction Method and Physiological Activity of High Content Oregonin Derived from Plant of Alnus sibirica Fisch. ex Turcz)

  • 최선은
    • 생약학회지
    • /
    • 제50권3호
    • /
    • pp.165-174
    • /
    • 2019
  • Oregonin is known as a representative active substance of plants of the Alnus species. In this study, we will show the results of establishing a method for extracting trace amounts of useful substances in plants. Additionally, a variety of physiological activity studies were conducted with extracts containing high content of useful substances. In this study, anti-oxidant, anti-inflammatory and anti-allergic activities of supercritical fluid residue 60% EtOH extracts of Alnus sibirica (ASCFR) were investigated in vitro. To explore anti-oxidant and anti-inflammatory characteristics of supercritical extract, DPPH radical scavenging, and ABTS radical scavenging activity, inhibition of nitric oxide production in LPS-stimulated RAW 264.7 cells and ${\beta}$-hexosaminidase release in IgE-stimulated RBL-2H3 cells were examined, respectively. Results showed a concentration-dependent increase in DPPH and ABTS radical scavenging activity. Cells stimulated with LPS produced more nitric oxide than normal control cells. However, cells treated with supercritical fluid residue 60% EtOH extract decreased production in a concentration-dependent manner. In anti-allergic activity studies, ASCFR showed concentration-dependentanti-allergic activity. Based on results and strong antioxidant activity, ASCFR has potential as basic research materials for development of therapeutic supplements-based medicines or functional cosmetics related to chronic inflammatory skin immunity diseases.

Effect of Carbonized Biomass Application on Organic Carbon Accumulation and Soy Bean Yields in Upland Soil

  • Lee, Sun-Il;Park, Woo-Kyun;Kim, Gun-Yeob
    • 한국토양비료학회지
    • /
    • 제49권1호
    • /
    • pp.1-6
    • /
    • 2016
  • Carbonized biomass could be used as a mechanism for long-term storage of C in soils. However, experimental results are variable. Objective of this study was carried out to evaluate the effect of carbonized biomass made from soybean residue on soil organic carbon and seed yield during soybean cultivation. The carbonized biomass was made by field scale mobile pyrolyzer. Pyrolyzer was performed in a reactor operated at $400{\sim}500^{\circ}C$ for 2 hours using soybean residue. The treatments consisted of four levels as the control without input and three levels of carbonized biomass inputs as $357kg\;ha^{-1}$, C-1 ; $714kg\;ha^{-1}$, C-2 ; $1,428kg\;ha^{-1}$, C-3. It was appeared that seed yield of soybean was $2,847kg\;ha^{-1}$ for control, $2,897kg\;ha^{-1}$ for C-1, $2,946kg\;ha^{-1}$ for C-2 and $3,211kg\;ha^{-1}$ for C-3 at the end of experiment. It was shown that the contents of SOC were $5.21g\;kg^{-1}$ for C-1, $5.93g\;kg^{-1}$ for C-2, $7.00g\;kg^{-1}$ for C-3 and $4.73g\;kg^{-1}$ for the control at the end of experiment. Accumulated SOC contents linearly significantly (P < 0.001) increased with increasing the carbonized biomass input. The slopes (0.00162) of the regression equations suggest that SOC contents from the soil increase by $0.162g\;kg^{-1}$ with every $100kg\;ha^{-1}$ increase of carbonized biomass rate. Consequently the carbonized biomass for byproducts such as soybean residue could increase SOC. It might be considered that the experimental results will be applied to soil carbon sequestration for future study. More long-term studies are needed to prove how long does SOC stay in agricultural soils.

서울지역 여고생의 배변 실태와 음료 섭취에 관한 연구 (The Relationship between the Prevalence of Constipation and Beverage Intake of Female High School Students in Seoul)

  • 임은태;김영남
    • 대한지역사회영양학회지
    • /
    • 제8권6호
    • /
    • pp.856-866
    • /
    • 2003
  • The purpose of this study was to study the relationship between the prevalence of constipation and beverage intake. Research data were collected from first and second grade female students at Hyehwa High School in Seoul by means of questionnaires. A total of 190 questionnaires were analyzed using the SPSS/WIN program. The results are summarized as follows: The average body mass index (BMI) was 20.7. Of the students, 38.4% had a BMI of less than 20(underweight), and 15.8% had a BMI of over 23 (overweight). However, 84.7% of the students wanted to lose weight. To estimate the prevalence of constipation, five factors were used: frequency and regularity of defecation, time spent defecating, defecation difficulty, and feeling of residue after defecation. The percentage of the students who emptied their bowels every day was 22.1%. About 2/5 of the students (42.1%) had irregular defecation, and 15.2% spent more than 10 minutes in the rest room when defecating. The students who did not have difficulty defecating and those who did not have feelings of residue after defecating were only 24.7% and 28.9%, respectively. The amount of beverage intake per day was 1,062$m\ell$ 674$m\ell$ of water, 128$m\ell$ of milk, 88$m\ell$ of soft drinks, and 53$m\ell$ of fruit juice. The amount of beverage intake before breakfast, during breakfast, before lunch and during lunch was about 100 $m\ell$, each; before dinner, during dinner, and after dinner it was about 200$m\ell$, each. The amount of beverage intake during the weekend was 120$m\ell$ greater than on weekdays. Students who drank more beverages, especially water, before breakfast, showed a higher defecation frequency, more regular defecation, spent less time defecating, had less difficulty defecating, and had less feeling of residue after defecation, so that they have a better defecation pattern. Based on these results, increasing the amount of beverage consumption, especially of water, and drinking greater quantities of beverage before breakfast are ways to reduce constipation.

폐자동차 파쇄잔재물(ASR) 재활용 시설의 에너지 회수효율 적용성 평가 (Applicability of the Energy Recovery in Automotive Shredder Residue (ASR) Recycling Facilities)

  • 유하녕;강준구;권영현;고영재;권준화;박호연;전태완;이영기
    • 한국폐기물자원순환학회지
    • /
    • 제35권7호
    • /
    • pp.660-669
    • /
    • 2018
  • Domestic automotive shredder residue (ASR) recycling facilities must comply with 60% of the energy recovery criteria calculated by the waste control act, based on resource circulation of electrical and electronic equipment and vehicles. The method of calculating energy recovery criteria was newly enacted on November 6, 2017, and it has been judged that it is necessary to consider applicability. In this study, the energy recovery efficiency of 7 units was calculated by past and present calculation methods. Furthermore, this study attempts to find applicability and a method of increasing the energy recovery efficiency by taking advantage of available potentials. An analysis of the calculation results showed that the average values calculated by past methods, present methods, and the method that includes available potentials are 76.35%, 70.68%, and 78.24%, respectively. Therefore, the new calculation method for energy recovery efficiency is also applicable to domestic automotive shredder residue recycling facilities.

생산단계 참외 중 Metalaxyl-M 및 Flusilazole의 잔류허용기준 설정연구 (Determination of Pre-Harvest Residue Limits of Pesticides Metalaxyl-M and Flusilazole in Oriental Melon)

  • 김다솜;김경진;김해나;김지윤;허장현
    • 농약과학회지
    • /
    • 제18권1호
    • /
    • pp.1-7
    • /
    • 2014
  • 본 연구는 참외에 대한 살균제 metalaxyl-M과 flusilazole의 잔류량 변화를 측정하여 약제별 잔류특성을 파악하고, 반감기를 산출함으로써 생산단계 잔류허용기준(PHRL, Pre-Harvest Residue Limit) 설정을 위한 기초자료로서 활용하고자 수행하였다. 공시 약제인 metalaxyl-M과 flusilazole은 안전사용기준에 준하여 7일 간격으로 1회, 2회살포하였으며, 살포 2시간 후를 0일차로 하여 0, 1, 2, 3, 5, 7, 9, 11일차에 수확하였고, 잔류량의 경시변화 결과를 토대로 생물학적 반감기를 산출하였다. 시료는 QuEChERS방법을 이용하여 전처리하였으며, metalaxyl-M의 경우 GC/NPD를 이용하여 분석하였으며, flusilazole은 LC/MS/MS를 이용하여 분석하였다. 참외 중 metalaxyl-M과 flusilazole의 LOQ (Limit of quantitation)는 각각 0.02 mg/kg, 0.01 mg/kg이었으며, metalaxyl-M 회수율은 95.7-103.2%이었고, flusilazole은 100.2-106.8%이었으며, 표준편차는 모두 10% 미만이었다. 참외 중 각 농약에 대한 생물학적 반감기는 flusilazole의 경우 1, 2회 처리구에서 12일이었으며, metalaxyl-M의 경우 23일이었다. 본 연구결과, metalaxyl-M 및 flusilazole의 수확 10일 전 PHRL은 각각 1.5, 0.3 mg/kg로 나타났으며, 수확 시 metalaxyl-M, flusilazole의 잔류농도는 MRL을 초과하지 않을 것으로 예측되었다.