• Korean Journal of Microbiology
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• v.30 no.6
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• pp.524-527
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• 1992

Synechococcus sp. cyanophage was isolated from Baekwoon reservoir located in KyonggiDo. The cyanophage was purified by employing ultrafiltration. differential centrifugation. and sucrose density gradient centrifugation. Electron microscopic observation indicated that the sizes of its isometric head and contractile tail are 89 nm and] II nm. respectively. which means that the isolated cyanophage is included in the group. Myoviridae. The cyanophage maintained the stability of more than 50 percent from $20^{\circ}C$ to $40^{\circ}C$ and from pH 5 to 8. and had the maximal infectivity at $30^{\circ}C$ and pH 9 implying its ecological significance.

• Korean Journal of Agricultural Science
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• v.40 no.3
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• pp.243-252
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• 2013

This study was carried out to investigate safety evaluation of agricultural reservoirs due to raising the embankment. The seepage analysis and large-scale model test were performed to compare and analyze the pore water pressure(PWP), leakage quantity, settlement and piping phenomenon in the inclined core type and the vertical core type embankments. The PWP after raising the embankment showed smaller than before raising the embankment and the stability for piping after raising the embankment. The allowable seepage quantity and the allowable leakage for the steady state and transient conditions is within the range of safe management standard. After raising the embankment in the inclined core, there was no infiltration by leakage. For the vertical core, the PWP showed a large change by faster infiltration of pore water than in the inclined core. In a rapid drawdown, inclined core was remained stable but the vertical core showed a large change in PWP. Settlement after raising the embankment showed larger amounts of settlement than before raising the embankment. The leakage quantity before raising the embankment and the inclined core type showed no leakage. From the result, an instrument system that can accurately estimate a change of PWP shall be established for the rational maintenance and stabilization of raising the embankment for agricultural reservoirs.

• Proceedings of the Korea Water Resources Association Conference
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• 2017.05a
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• pp.433-433
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• 2017

본 연구에서는 노후된 소규모 농업용저수지의 홍수에 대한 안전성이 부족하여 최근들어 노후된 소규모 농업용 저수지를 재설계하여 치수능력을 보강하고 있다. 이러한 노후 소규모 농업용 저수지의 홍수량을 재산정하기 위해 강우량을 수집하여 확률강우량을 재산정하고 빈도별 홍수량을 산정하였다. 산정된 빈도별 확률홍수량을 이용하여 현재의 치수적 안정성을 검토하고 현재기준에 적합한 저수지의 치수적 안정성을 갖추기 위해 댐을 증고하는 방안에 대해 검토하였다. 본 연구에서 저수지의 안정성을 검토한 결과 심천저수지의 홍수위는 200년 빈도에서 EL. 124.38m, 300년 빈도에서 EL. 124.43m 500년 빈도에서 EL. 124.48m로 현재 댐마루고 EL. 125.60m와 비교시 월류는 하지 않으나 댐설계기준(2011,국토해양부)에서 제시된 식에 따른 여유고와 비교한 결과 현재 상태에서는 여유고가 부족하여 증고가 필요한 것으로 분석되었다. 따라서 심천저수지의 현재 홍수량에 대한 검토를 위해 100년, 80년, 50년 빈도의 저수지 홍수추적결과 심천저수지의 여유고는 100년 빈도에서 0.028m부족, 80년 빈도에서 0.007m부족, 50년 빈도에서 기준여유고를 0.046m 만족하여 현재 상태에서는 50년 빈도에서 안정한 것으로 검토되었다.

• Geomechanics and Engineering
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• v.34 no.1
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• pp.43-49
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• 2023

Knowledge of minimum horizontal stress (S_hmin) is a significant step in determining full stress tensor. It provides crucial information for the production of sand, hydraulic fracturing, determination of safe mud weight window, reservoir production behavior, and wellbore stability. Calculating the S_hmin using indirect methods has been proved to be awkward because a lot of data are required in all of these models. Also, direct techniques such as hydraulic fracturing are costly and time-consuming. To figure these problems out, this work aims to apply the long-short-term memory (LSTM) algorithm to S_hmin time-series prediction. 13956 datasets obtained from an oil well logging operation were applied in the models. 80% of the data were used for training, and 20% of the data were used for testing. In order to achieve the maximum accuracy of the LSTM model, its hyper-parameters were optimized significantly. Through different statistical indices, the LSTM model's performance was compared with with other machine learning methods. Finally, the optimized LSTM model was recommended for S_hmin prediction in the well logging operation.

• The Journal of Engineering Geology
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• v.9 no.3
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• pp.207-225
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• 1999

Twenty water samples (eleven groundwater and nine geothermal water samples) were collected to elucidate hydrogeochemical characteristics of the groundwater and geothermal water in the Dongrae hot-spring area and its vicinity. Major and minor elements were analyzed for ground and geothermal water samples. Physicochemical properties of the groundwater and the geothermal water were examined and chemical composition of the two waters were compared. Factor and correlation analyses were carried out to simplify the physicochemical data into grouping some factors and to find interaction between them. The groundwaters belong to $Ca-HCO_3$ type, while the geothermal waters belong to Na-Cl type. The Na and Cl concentrations in the Dongrae hot-spring area are higher than those of other granite areas in South Korea. The Na/Cl weight ratio ranges from 0.7 to 1.3 for the geothermal waters. On the phase stability diagram groundwaters fall effectively in the field of stability of kaolinite, while geothermal waters fall in the stability field of microcline or kolinite depending on the chemical composition system. Based on the Na-K, Na-K-Ca and Na-K-Ca-Mg geothermometers, the geothermal reservoir is estimated to have equilibrium temperature between 115 and $145^{\circ}C$.

• Proceedings of the Korean Vacuum Society Conference
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• 2013.08a
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• pp.273-273
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• 2013

Recently, Point-of-care (POC) testing microdevices enable to do the patient monitoring, drug screening, pathogen detection in the outside of hospital. Immunochromatographic strip (ICS) is one of the diagnostic technologies which are widely applied to POC detection. Relatively low cost, simplicity to use, easy interpretations of the diagnostic results and high stability under any circumstances are representative advantages of POC diagnosis. It would provide colorimetric results more conveniently, if the genetic analysis microsystem incorporates the ICS as a detector part. In this work, we develop a reverse transcriptase-polymerase chain reaction (RT-PCR) microfluidic device integrated with a ROSGENE strip for colorimetric influenza H1N1 virus detection. The integrated RT-PCR- ROSGENE device is consist of four functional units which are a pneumatic micropump for sample loading, 2 ${\mu}L$ volume RT-PCR chamber for target gene amplification, a resistance temperature detector (RTD) electrode for temperature control, and a ROSGENE strip for target gene detection. The device was fabricated by combining four layers: First wafer is for RTD microfabrication, the second wafer is for PCR chamber at the bottom and micropump channel on the top, the third is the monolithic PDMS, and the fourth is the manifold for micropump operation. The RT-PCR was performed with subtype specific forward and reverse primers which were labeled with Texas-red, serving as a fluorescent hapten. A biotin-dUTP was used to insert biotin moieties in the PCR amplicons, during the RT-PCR. The RT-PCR amplicons were loaded in the sample application area, and they were conjugated with Au NP-labeled hapten-antibody. The test band embedded with streptavidins captures the biotin labeled amplicons and we can see violet colorimetric signals if the target gene was amplified with the control line. The off-chip RT-PCR amplicons of the influenza H1N1 virus were analyzed with a ROSGENE strip in comparison with an agarose gel electrophoresis. The intensities of test line was proportional to the template quantity and the detection sensitivity of the strip was better than that of the agarose gel. The test band of the ROSGENE strip could be observed with only 10 copies of a RNA template by the naked eyes. For the on-chip RT-PCR-ROSGENE experiments, a RT-PCR cocktail was injected into the chamber from the inlet reservoir to the waste outlet by the micro-pump actuation. After filling without bubbles inside the chamber, a RT-PCR thermal cycling was executed for 2 hours with all the microvalves closed to isolate the PCR chamber. After thermal cycling, the RT-PCR product was delivered to the attached ROSGENE strip through the outlet reservoir. After dropping 40 ${\mu}L$ of an eluant buffer at the end of the strip, the violet test line was detected as a H1N1 virus indicator, while the negative experiment only revealed a control line and while the positive experiment a control and a test line was appeared.

• Journal of Korea Water Resources Association
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• v.37 no.7
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• pp.569-579
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• 2004

Recently, the extreme drought is often occurred due to the global warming and the serious weather changes. Also, the problems of the water pollution In the developed areas, the oppositions from people in the upper stream area and water concession from the local governments affect the national request to get more clean water resources in upper stream of the undeveloped areas. It also brings on the necessity of recognition for water supply managements. Therefore, as the water demand is rapidly changes in the metropolitan areas, the capability of water supply from the north Han river basin dams should be appropriately investigated. In this study, we developed a simulation system using STELLA (equation omitted) software environment, a shared vision model, to analyze the possibility of the stable water supply from north Han river basin dams. Also, three different rules are applied on this model by dividing the water level to minimum(Rule 1), medium(Rule 2) and maximum(Rule 3). Using the rules, the safety yield changes are analyzed for dam rule curve of the reservoir and hydropower release.

• The Journal of Engineering Geology
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• v.26 no.4
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• pp.497-503
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• 2016

The cement grouting by vibration method (CGVM), a new construction technology developed in Korea, aims to efficiently reinforce a target ground area by injecting vibrated grout into it. The present study applies the CGVM to test sites and verifies its effect. Standard penetration tests (SPTs), field permeability tests, and geophysical surveys were conducted in two study areas, and the results were compared between before and after CGVM application. The SPT conducted before and after the CGVM application showed that the N value was increased by 33.57% point to 60.90% point. The field permeability test showed that the permeability coefficient decreased. These results indicate that CGVM may increase imperviousness and improve reservoir system stability. In addition, a resistivity survey found no low-resistivity zone, unlike before the CGVM application, thereby indicating the excellent grouting effect of the CGVM.

• Tunnel and Underground Space
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• v.27 no.2
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• pp.77-88
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• 2017

The geology of the study area is composed mainly of conglomerate, sandstone, and shale and basalt. It is a rock that has been observed to move relatively recently through various brittle deformation and various stress fields during the recent period. To form a gentle terrain with severe crushing. The slope is located at the intersection of the Taegok Fault in the north-northeast direction and the Bukok Fault in the western north-west direction, and many faults, fault zones and fracture zones of various sizes are developed in the rock bed. In this study, the geological characteristics of the slope are investigated and the countermeasure method is suggested. It is suggested that periodical measurement and analysis should be performed by installing a measuring instrument according to each structure for safety management of the surrounding roads and grounds during construction or reinforcement by the countermeasure method for the slope of the study area.

• Journal of Korean Society of Water and Wastewater
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• v.27 no.1
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• pp.1-10
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• 2013

Major issues in water supply service have changed from expansion of service area to improvement of service quality, i.e., water quality and safety, and early response to emergency situation. This change in the service concept triggers the perceptions of limitation with the current centralized water supply system and of necessities of decentralized (distributed) water supply system (DWSS), which can make up the limitations. DWSS can reduce the possibility of water supply outage by establishing multiple barriers such as emergency water supply system, and secure better water quality by locating treatment facilities neighboring consumers. On the other hand, fluctuation of water demand will be increased due to the reduced supply area, which makes difficult to promptly respond the fluctuating demand. In order to supplement this, hybrid water supply system was proposed, which combined DWSS with conventional water supply system using distributing reservoir to secure the stability of water supply. The Optimal connection point of DWSS to existing water supply network in urban area was determined by simulating a supply network using EPANET. Optimal location of decentralized water treatment plant (or connection point) is a nodal point where changes in pressure at other nodal points can be minimized. At the same time, the optimal point should be selected to minimize hydraulic retention time in supply network (water age) to secure proper water quality. In order to locate the point where these two criteria are satisfied optimally, Distance measure method, one of multi-criteria decision making was employed to integrate the two results having different dimensions. This methodology can be used as an efficient decision-support criterion for the location of treatment plant in decentralized water supply system.