• BMB Reports
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• 제30권4호
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• pp.299-301
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• 1997

The asialoglycoprotein receptor (ASGPR) was the first described mammalian lectin that mediates the specific binding and internalization of galactose/N-acetylgalactosamine-terminating glycoproteins by hepatic parenchymal cells. H1 and H2 are known as essential subunits of the functional ASGPR. There were close similarities in ASGPR H2 subunits between cultured cell line HepG2 and normal human liver cells including identical sequences at both termini. It was therefore expected that there may be some similarities between the subunits from normal liver cells and fetal liver cells. The two subunits of human fetal liver ASGPR. designated FL-H1 and FL-H2. were cloned from cDNA library by peR and the sequences were compared with the known HI and H2 sequences of HepG2, and the H1 sequence of nornal human liver cells. The results showed that FL-H1 was identical to H1 of HepG2. Whereas FL-H2 contains a 15-bp miniexon, but missing 57-bp at the near upstream from the membrane-spanning domain compared to H2 of HepG2 and normal human liver cells indicating that FL-H2 resulted from a differential splicing compared to HepG2 and normal liver cells.

• 한국환경과학회지
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• 제23권1호
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• pp.7-23
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• 2014

This study estimates unit for the nonpoint source(NPS), classified according to the existing Level-1(large scale) land cover map, by monitoring the measurement results from each Level-2(medium scale) land cover map, and verifies the applicability by comparison with previously calculated units using the Level-1 land cover map. The NPS pollutant loading for a basin is evaluated by applying the NPS pollutant unit to Dongcheon basin using the Level-2 land cover map. In addition, the BASINS/HSPF(Better Assessment Science Integrating point & Non-point Sources/Hydrological Simulation Program-Fortran) model is used to evaluate the reliability of the NPS pollutant loading computation by comparing the loading during precipitation in the Dongcheon basin. The NPS pollutant unit for the Level-2 land cover map is computed based on precipitation measured by the Sangju observatory in the Nakdong River basin. Finally, the feasibility of the NPS pollutant loading computation using a BASINS/HSPF model is evaluated by comparing and analyzing the NPS pollutant loading when estimated unit using the Level-2 land cover map and simulated using the BASINS/HSPF models.

• BMB Reports
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• 제29권6호
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• pp.545-548
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• 1996

CA(1-8)ME(1-12), the CA-ME hybrid peptide of the amino terminal segments of cecropin A (CA) and melittin (ME), has been reported to have a broad spectrum and improved potency without a hemolytic property. In order to obtain new synthetic peptides with powerful antibacterial activity without hemolytic activity, several hybrid peptides were designed from the sequences of CA, ME, magainin 2, bombinin and lactoferricin. All hybrid peptides were constructed to form an amphipathically basic-flexible-hydrophobic structure and synthesized by the solid phase method. Their hemolytic activities against human red blood cells and antibacterial activities against both Gram-positive and Gram-negative bacteria were detennined. CA(1-8)MA(1-12), CA(1-8)BO(1-12), MA(10-17)ME(1-12) and LF(20-29)ME(1-12) showed comparable activities with broad spectra against both Gram-positive and Gram-negative bacteria relative to CA(1-8)ME(1-12) but without hemolytic properties. These hybrid peptides, therefore, could be useful as model peptides to design a novel peptide with improved antibacterial activity and study on structure-activity relationships of antimicrobial peptides.

• Journal of Microbiology and Biotechnology
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• 제9권5호
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• pp.613-618
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• 1999

L-Proline-producing mutant strains were developed by exposing L-glutamic acid-producing bacteria to N-metyl-N-nitro-nitrosoguanidine and UV irradiation. A L-histidine auxotroph of Corynebacterium acetoacidophilum RYU3161(KCTC 0616BP), which was resistant to sulfaguanidine and proline analogs (DHP, AZC, TAC), was isolated. The activity of the mutant strain's $\gamma$-glutamyl kinase was 45% higher than that of the parent strain. The optimum level of L-histidine for production of L-proline was 0.16 g/l. In a 5-1 jar fermenter, the mutant strain produced L-proline at a high concentration (35 g/l) level within 48 h of cultivation.

• 생약학회지
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• 제28권4호
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• pp.179-184
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• 1997

To search for useful antioxidants from plant materials, we investigated the antioxidative activity in the methanol extracts of 30 Chinese medicinal plants using DPPH method. The highest activity $(RC_{50}:\;12\;{\mu}g)$ was showed in the methanol extract of Rosa rugosa, followed by Potentilla fruticosa $(14\;{\mu}g)$, P. fragarioides $(16\;{\mu}g)$. And Geum aleppicum $(18\;{\mu}g)$. From the leaves of R. rugosa, two antioxidative compounds were isolated by a bioassay guided purification and identified as isoquercitrin and ${\beta}-glucogallin$ on the basis of $^1H-,\;^{13}C-\;NMR$, and FAB-MS data. ${\beta}-Glucogallin$ is the first report in this plant. The DPPH radical scavenging activity of ${\beta}-glucogallin\;(RC_{50}:\;8.5\;{\mu}g)$ was more effective than those of ${\alpha}-tocopherol\;(12\;{\mu}g)$ and BHA $(14\;{\mu}g)$.

• 식물조직배양학회지
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• 제25권6호
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• pp.501-505
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• 1998

오이의 국내 F1 품종인 조생낙합의 하배축 유래 배발생 현탁배양 세포의 초저온 보존 시스템을 개발하였다. 액체질소 저장 후 캘러스 재생률은 2M DMNSO와 0.4 M sucrose를 혼용 처리하였을 때 캘러스 재생률이 85%로 가장 높았다. 그러나 glycerol 처리구에서는 처리농도에 상관없이 모든 처리구에서 캘러스 재생이 이루어지지 않았다. 또한 고농도의 삼투용액에서 배양세포의 전처리 과정은 필요하지 않았다. 재생된 캘러스를 1 mg/L 2,4-D가 첨가된 MS 배지로 이식하여 배양하였을 때 다수의 체세포배가 발달하였으며, 체세포배를 MS 기본배지로 옮겨 명배양한 결과 다수의 소식물체가 발달하였다.

• Clinics in Shoulder and Elbow
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• 제24권2호
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• pp.98-105
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• 2021

Background: Our aim is to determine the interobserver reliability for surgeons to detect Hill-Sachs lesions on magnetic resonance imaging (MRI), the certainty of judgement, and the effects of surgeon characteristics on agreement. Methods: Twenty-nine patients with Hill-Sachs lesions or other lesions with a similar appearance on MRIs were presented to 20 surgeons without any patient characteristics. The surgeons answered questions on the presence of Hill-Sachs lesions and the certainty of diagnosis. Interobserver agreement was assessed using the Fleiss' kappa (κ) and percentage of agreement. Agreement between surgeons was compared using a technique similar to the pairwise t-test for means, based on large-sample linear approximation of Fleiss' kappa, with Bonferroni correction. Results: The agreement between surgeons in detecting Hill-Sachs lesions on MRI was fair (69% agreement; κ, 0.304; p<0.001). In 84% of the cases, surgeons were certain or highly certain about the presence of a Hill-Sachs lesion. Conclusions: Although surgeons reported high levels of certainty for their ability to detect Hill-Sachs lesions, there was only a fair amount of agreement between surgeons in detecting Hill-Sachs lesions on MRI. This indicates that clear criteria for defining Hill-Sachs lesions are lacking, which hampers accurate diagnosis and can compromise treatment.

• 대한원격탐사학회:학술대회논문집
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• 대한원격탐사학회 2002년도 Proceedings of International Symposium on Remote Sensing
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• pp.228-228
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• 2002

The DLS(Data Link System) is located in the PDTS(Payload Data Transmission Subsystem) of KOMPSAT-II, and its main function is to provide communication link with Ground Segment as a space segment. DLS receive the data of MSC, OBC from DCSU(Data Compression Storage Unit) and transmit to the Ground Station by X-Band RF link. DLS is consist of CCU(Channel Coding Unit), QTX(QPSK Transmitter, ASU(Antenna Switch Unit) CCU makes a packet for communication after several kind of data processing such like Ciphering, RS Coding. QTX transmit PDTS data by OQPSK. Modulation. ASU is the unit for reliability of antenna switching. So, DLS's function is consists of ciphering, RS coding, CCSDS packetizing, randomizing, modulation and switching to antenna. These DLS's functions are controlled by PMU(Payload Management Unit). All commands to DLS are sent by PMU and all telemetries of DLS are sent to the PMU. The PMU receives commands from OBC and sends telemetries to the OBC. The OBC communicates with Ground Station by S-Band RF link. This paper presents the on-orbit DLS operation concept through the ground segment.

• 한국물환경학회지
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• 제27권5호
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• pp.719-728
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• 2011

The results of investigation on the water quality of the unit watersheds in the Yeongsang/Seomjin River indicate that $BOD_5$, SS, T-P decrease in most of the unit watersheds. However, $COD_{Mn}$ and T-N increase since the Total Maximum Daily Loads (TMDLs). It is thought that $COD_{Mn}$, which is included non-biodegradable matters, is difficult to decrease only using by conventional biological treatment facilities and T-N is affected by non-point source, etc. The results of assessment on 3 years $BOD_5$ indicate that most of the unit watersheds were being improved except Yeongbon A, Seombon C and Yocheon B. In addition, it was found that T-P were also being improved except Yocheon B and Hwangryeong A. Consequently, water qualities of the unit watersheds have been improved in many cases since the TMDLs.

• 대한임상약리학회:학술대회논문집
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• 대한임상약리학회 2002년도 제11차 추계학술대회
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• pp.8-8
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• 2002