Isolation of a High-Yield Mutant Strain for L-Proline Production and Its Fermentation Conditions

  • Ryu, Wuk-Sang (Biochemical Process Engineering Research Unit, Korea Research Institute of Bioscience and Biotechnology) ;
  • Jang, Hyung-Wook (Biochemical Process Engineering Research Unit, Korea Research Institute of Bioscience and Biotechnology) ;
  • Cho, Kyoung-Hee (Biochemical Process Engineering Research Unit, Korea Research Institute of Bioscience and Biotechnology) ;
  • Chang, Soon-Jae (Yang Ji Chemical Co., Ltd.) ;
  • Ryu, Yeon-Woo (Department of Molecular Science and Technology, Ajou University) ;
  • Park, Young-Hoon (Biochemical Process Engineering Research Unit, Korea Research Institute of Bioscience and Biotechnology)
  • 발행 : 1999.10.01

초록

L-Proline-producing mutant strains were developed by exposing L-glutamic acid-producing bacteria to N-metyl-N-nitro-nitrosoguanidine and UV irradiation. A L-histidine auxotroph of Corynebacterium acetoacidophilum RYU3161(KCTC 0616BP), which was resistant to sulfaguanidine and proline analogs (DHP, AZC, TAC), was isolated. The activity of the mutant strain's $\gamma$-glutamyl kinase was 45% higher than that of the parent strain. The optimum level of L-histidine for production of L-proline was 0.16 g/l. In a 5-1 jar fermenter, the mutant strain produced L-proline at a high concentration (35 g/l) level within 48 h of cultivation.

키워드

참고문헌

  1. Biotechnology of Amino Acids Production Aida, K.;L. Chibita;K. Nakayama;K. Takinami;H. Yamada
  2. Agric. Bio. Chem. v.39 Fermentation production of L-proline with auxotrophs of Corynebacterium glutamicum Araki, K.;Y. Takasawa;J. Nakajima
  3. Biochem. Biophys. Acta. v.192 Proline synthesis in Escherichia coli: A proline-inhibitable glutamic acid kinase Baich, A.
  4. Biochem. Biophys. Acta. v.104 Control of proline synthesis in Escherichia coli Baich, A.;D. J. Pierson
  5. J. Microbiol. Biotechnol. v.7 Sustained production of amino acids by immobilized analogue-resistant mutants of a Cyanobacterium Anacystis nidulans BD-1 Bagchi, S. Nath;N. S. Rao
  6. Manual of Methods for General Bacteriology Gene mutation Carlton, B. C.;B. J. Brown;P. Gerhardt(ed.)
  7. J. Biol. Chem. v.199 Photometric estimation of proline and ornithine Chinard, F. P.
  8. Biochem. J. v.173 The enzyme of proline biosynthesis in Escherichia coli Hyzer, D. J.;V. Moses
  9. Crit. Rev. Biotechnol. v.15 Recent advances in the physiology and genetics of amino acid producing bacteria Jettern, M. S. M.;A. J. Sinskey
  10. Appl. Microbiol. v.23 Effect of L-aspartic acid and L-glutamic acid on production of L-proline Kato, J.;M. Kisumi;I. Chibata
  11. Biology of Industrial Microorganisms L-Glutamic acid bacteria Kimoshita, S.;Demain, A. L.(ed.);N. A. Slolmon(ed.)
  12. The Nicrobial Production of Amino Acid Kinoshita, S.;K. Tanaka
  13. Experiments in Molecular Genetics Miller, J. H.
  14. J. Biol. Chem. v.46 Fermentation production of L-proline by DL-3,4-dehydro proline resistant mutant of L-glutamate producing bacteria Nakamori, S.;H. Moriola;F. Yoshinaga;S. Yamanaka
  15. Amino acid products and technology Ruffio, P.
  16. Metabolism at a Glance Salway, J. G.
  17. Moilecular Cloning. A Laboratory Manual Appendix 3(2nd ed.) Sambrook, J.;E. F. Fritch;T. Maniatis
  18. J. Gen. Appl. Microbiol. v.17 Microbioal production of alanine, L-proline and L-valine by theronine or methionine Sensitive mutants of Brevibacterium flavum Sano, K.;I. Shiio
  19. J. Biochem. v.51 Free intracellular amino acid of a glutamate-forming bacterium. Breibacterium flavum No. 2247 Shiio, I.;K. Narui;N. Yahaba;M. Takahashi
  20. Appl. Environ. Microbiol. v.49 Proline-hyperproducing strain of Serratia marcescens: Enhancement of proline analog-mediated growth inhibition by increasing osmotic stress Sugiura, M.;M. Kisumi
  21. Agric. Biol. Chem. v.50 Improvement of L-proline production by sulfaguanidine resistant mutants derived from L-glutamic acid producing bacteria Tsuchida, T.;K. Kubota;F. Yoshinaga
  22. J. Am. Chem. Soc. v.78 Ornithine-proline interrelation in Escherichia coli Vogel, H. J.
  23. Biochem. v.40 On the glutamate-proline-ornithine interrelation in Neurospora crassa Vogel, H. J.;D. M. Bonner
  24. J. Am. Chem. Soc. v.74 Proline biosynthesis Vogel, H. J.;B. D. Davis
  25. Amino Acid Metabolism Vogel, H. J.;W. D. McElroy;B. Glass
  26. J. Gen. Appl. Microbiol. v.15 Studies on the fermentative production of L-proline, Ⅳ. Mechanism of L-proline production by Brevibacterium flavum 2247 No.14-5 Yoshinaga, F.
  27. J. Gen. Appl. Microbiol. v.12 Study on the fermentative production of L-proline, Ⅰ. Production of L-proline by an isoleucine auxotropic mutant of Brevibacterium flavum 2247 Yoshinaga, F.;S. Konishi;S. Okumura;N. Katsuya
  28. Biochem. Biophys. Res Commun. v.27 Glutamate kinase activity in Brevibacterium flavum: Relationship between L-proline and L-glutamine biosynthesis Yoshinaga, F.;Y. Takeda;S. Okumura
  29. Agric. Bio. Chem. v.39 Purification and properties of glutamate kinase required for L-proline and L-glutamine biosynthesis Yoshinaga, F.;T. Tsuchida;S. Okumura
  30. J. Gen. Appl. Microbiol. v.13 no.14-15 Study on the fermentative production of L-proline, Ⅱ. Conditions for L-proline production by brevibacterium flavum 2247 Yoshinaga, F.;Y. Yoshihara;S. Konishi;S. Okumura;N. Katsuya
  31. Genetic Engineering of Gram Positive Bacteria in Biotechnology Whollen, W.;G. Muth;J. Kalinowski;Rehm, H. J.(eds.);G. Reed(eds.);A. Puehler(eds.);H. Sahm(eds.)