• Microbiology and Biotechnology Letters
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• v.36 no.1
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• pp.12-20
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• 2008

Validation of viral safety is essential in ensuring the safety of mammalian cell culture-derived biopharmaceuticals, because numerous adventitious viruses have been contaminated during the manufacture of the products. Mammalian cells are highly susceptible to minute virus of mice(MVM), and there are several reports of MVM contamination during the manufacture of biopharmaceuticals. In order to establish the validation system for the MVM safety, a real-time PCR method was developed for quantitative detection of MVM in cell lines, raw materials, manufacturing processes, and final products as well as MVM clearance validation. Specific primers for amplification of MVM DNA was selected, and MVM DNA was quantified by use of SYBR Green I. The sensitivity of the assay was calculated to be $6{\times}10^{-2}TCID_{50}/mL$. The real-time PCR method was proven to be reproducible and very specific to MVM. The established real-time PCR assay was successfully applied to the validation of Chinese hamster ovary (CHO) cell artificially infected with MVM. MVM DNA could be Quantified in CHO cell as well as culture supernatant. When the real-time PCR assay was applied to the validation of virus removal during a virus filtration process, the result was similar to that of virus infectivity assay. Therefore, it was concluded that this rapid, specific, sensitive, and robust assay could replace infectivity assay for detection and clearance validation of MVM.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.10 no.10
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• pp.2786-2793
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• 2009

Recently, the development and spread of the new recyclable energy becomes urgent because of the depletion of fossil fuel and strengthening the environmental regulation. To recovery from the waste out of the many new recyclable energies has been proved as the most favorable when the potential value of energy source is compared. The RDF from the waste has been approved as the most economical method out of the other methods. However, the toxic gases (HCl, Dioxin etc) and heavy metals generated during the burning of the industrial wastes have been pointed out as problems. The PVC, alkali metal chloride, and alkaline earth metal chloride are major materials for emitting the chlorine and chlorine compounds have the problem such as the erosion on the heat collection device. This research has analyzed the heavy metal components containing in the industrial waste, and the concentration of Cl and S in the industrial waste generated in B industrial complex are slightly high than that of the A industrial complex. The results can be used to discuss the origin of inorganic components in industrial waste and utilized as a base data to improve the performance of the RDF as fuel.

• Journal of the Ergonomics Society of Korea
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• v.32 no.5
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• pp.437-442
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• 2013

Objective: The objective of this study was to investigate the effects of drive-assisting system in a shower carrier on the upper body muscle activities of caregivers through drivability tests. Background: In care facilities, one of the major ADL (Activities of Daily Living) factors is bathing/showering. Recently, bath/shower-assisting equipment is actively being introduced in care facilities to reduce caregivers' muscle burden. In particular, it is desirable to utilize a shower carrier equipped with drive-assisting system to effectively care for the elderly. However, there were few systematic studies on the relationship between muscle activities and drive-assisting speeds. Method: For the drivability tests to study the effects on the muscle activities according to the drive-assisting speeds(corresponding drive-voltages: 0.0V, 2.0V, 2.1V, 2.3V), 6 females in their 40s($43{\pm}4yrs$, $157{\pm}5cm$, and $54.5{\pm}1.5kg$) were selected. To measure muscle activities of caregivers through drivability tests, 7 muscles in the upper body(TM/Trapezius Muscle, DM/Deltoid Muscle, BBM/Biceps Brachii Muscle, TBM/Triceps Brachii Muscle, ECRLM/Extensor Carpi Radialis Longus Muscle, FCUM/Flexor Carpi Ulnaris Muscle, and ESM/Erector Spinae Muscle) were selected. Results: In the TM, muscle activities were decreased as 21% compared to 0.0V, when drive-voltage 2.0V was applied, as 57% by 2.1V, and 62% by 2.3V(p<0.05), whereas 40%, 56%, and 69% of muscles activities were decreased respectively from the DM(p<0.05). Also, from the UL(BBM+TBM+ECRLM+FCUM), muscle activities were decreased by 17% with 2.0V as against 0.0V, by 47% with 2.1V, and 52% with 2.3V, whereas decreases in muscle activities from the ESM were found by 20%, 34%, and 42% respectively by 2.0V, 2.1V, and 2.3V(p<0.05). Conclusion: The muscle activities were decreased in the order of the DM, TM, ESM, and UL. As muscle activities were remarkably reduced as drive voltage were increased, it was expected to reduce the upper body muscle burden on the caregivers when using shower carriers equipped with driving-assist system. Applications: The results from this study can be applied for the development of a shower carrier including other equipment to possibly reduce the muscle burden of the caregivers.

• Korean Journal of Food Science and Technology
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• v.52 no.2
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• pp.177-182
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• 2020

This study evaluated the effect of steam heating, gamma irradiation, and ultraviolet (UV) irradiation on microorganism reduction in order to determine an effective sterilization method for red pepper powder. The effect of each treatment on the reduction of thermoduric bacteria and total aerobic bacteria in red pepper powder were as follows: 10 kGy gamma irradiation, reduction of 4 log and 6 log CFU/g, respectively; 12 mW/㎠ UV irradiation (264 nm UV-C), reduction of less than 1 log CFU/g; steam heating at 120℃ for 40 s, reduction of approximately 2 log CFU/g. High-temperature short-time processing at 110℃ for 30 s reduced the total bacterial count in Gochujang solution from 5.70 log CFU/g to 2.26 log CFU/g; at 121℃, the solution was commercially sterile. Steam heating resulted in 1, 2, and 4 log microbial inactivation in garlic, onion, and pepper powder, respectively. Steam sterilization, which consumers prefer over other methods, may be an effective method for reducing microorganisms in spice powders, including those in red pepper powder.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.1 no.1
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• pp.21-32
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• 1981

Recent trends in design standards development in some European countries and U.S.A. have encouraged the use of probabilistic limit sate design concepts. Reliability based design criteria such as LSD, LRFD, PBLSD, adopted in those advanced countries have the potentials that they afford for symplifying the design process and placing it on a consistent reliability bases for various construction materials. A reliability based design criteria for RC flexural members are proposed in this study. Lind-Hasofer's invariant second-moment reliability theory is used in the derivation of an algorithmic reliability analysis method as well as an iterative determination of load and resistance factors. In addition, Cornell's Mean First-Order Second Moment Method is employed as a practical tool for the approximate reliability analysis and the derivation of design criteria. Uncertainty measures for flexural resistance and load effects are based on the Ellingwood's approach for the evaluation of uncertainties of loads and resistances. The implied relative safety levels of RC flexural members designed by the strength design provisions of the current standard code were evaluated using the second moment reliability analysis method proposed in this study. And then, resistance and load factors corresponding to the target reliability index(${\beta}=4$) which is considered to be appropriate level of reliability considering our practices are calculated by using the proposed methods. These reliability based factors were compared to those specified by our current ultimate strength design provisions. It was found that the reliability levels of flexural members designed by current code are not appropriate, and the code specified resistance and load factors were considerably different from the reliability based resistance and load factors proposed in this study.

• Journal of Korea Spatial Information System Society
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• v.11 no.1
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• pp.43-54
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• 2009

As R&D(Research and Development) is going on actively to develop technologies for the ubiquitous computing environment, which Is the human-oriented future computing environment, GIS dealing with spatio-temporal data is emerging as a promising technology. This also increases the necessity of the middleware for providing services to give interoperability in various heterogeneous environments. The core technologies of the middleware are real-time processing technology of data streams coming unceasingly from positioning systems and data stream processing technology developed for non-spatio-temporal data. However, it has problems in processing queries on spatio-temporal data efficiently. Accordingly, this paper designed and implemented the spatio-temporal middleware that provides interoperability between a mobile spatio-temporal DBMS(DataBase Management System) and a server spatio-temporal MMDBMS(Main Memory DataBase Management System). The spatio-temporal middleware maintains interoperability among heterogeneous devices and guarantees data integrity in query processing through real-time processing of unceasing spatio-temporal data streams and two way synchronization of spatio-temporal DBMSs. In addition, it manages session for the connection of each spatio-temporal DBMS and manages resources for its stable operation. Finally, this paper proved the usability of the spatio-temporal middleware by applying it to a real-time position tracking system.

• The Korean Journal of Pesticide Science
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• v.9 no.3
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• pp.221-230
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• 2005

Temperature is an important ambient factor affecting the physiology and metabolism of aquatic invertebrates. In this study, we studied about the survival, reproduction and growth effects of 4 different temperatures(16, 20, 24, $28^{\circ}C$) in four Korean cladoceran, Daphnia sp., Daphnia obtusa, Moina macrocopa, Simocephalus vetulus and Daphnia magna as an international standard species. All 5 water flea tested showed that molting time, fecundity and intrinsic rate of natural increase(r) in high temperature condition were higher than those in lower temperature. On the other hand, lower survivals and longer time to start of offspring were showed in high temperature. Our results suggest that the optimal temperatures of Daphnia sp.,, Moina macrocopa and Simocephalus vetulus seem to be about $20^{\circ}C$ except for Daphnia obtusa which as showed good survivals and reproductions in $16^{\circ}C$.

• The Journal of the Institute of Internet, Broadcasting and Communication
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• v.13 no.5
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• pp.17-26
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• 2013

ISO/IEC 29157 originally developed in the Republic of Korea and is based on commercially available PicoCast v1.0. ISO/IEC JTC1 SC6 was registered by the international standard on May 2010. A single platform for a variety of applications and media formats to support development objectives were. ISO/IEC 29157 based wireless networks, ie, Pico-net to master node periodically transmit sync signal is synchronized to the number of slave nodes have the communications structure. Pico-net also supports a variety of network topologies and direct communication between nodes(single-hop communication) and QoS is guaranteed. But Pico-net network structure has the following problems. Loss of communication problems due to mobile nodes, resulting in limitations of node mobility and wireless network operation range of conventional wireless networks operating range less than 1/4 was reduced to the problem. In this paper, a possible solution to the problems mentioned is proposed, using multi-hop communication technology and sync signal transmission technology between nodes.

• Korean Chemical Engineering Research
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• v.57 no.4
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• pp.475-483
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• 2019

Special chemical warfare agents are lethal gases that attack the human respiratory system. One of such gases are blood agents that react with the irons present in the electron transfer system of the human body. This reaction stops internal respiration and eventually causes death. The molecular sizes of these agents are smaller than the pores of an activated carbon, making chemical adsorption the only alternative method for removing them. In this study, we carried out a Computational Fluid Dynamics simulation by passing a blood agent: cyanogen chloride gas through an SG-1 gas mask canister developed by SG Safety Corporation. The adsorption bed consisted of a Silver-Zinc-Molybdenum-Triethylenediamine activated carbon impregnated with copper, silver, zinc and molybdenum ions. The kinetic analysis of the chemical adsorption was performed in accordance with the test procedure for the gas mask canister and was validated by the kinetic data obtained from experimental results. We predicted the dynamic behaviors of the main variables such as the pressure drop inside the canister and the amount of gas adsorbed by chemisorption. By using a granular packed bed instead of the Ergun equation that is used to model porous materials in Computational Fluid Dynamics, applicable results of the activated carbon were obtained. Dynamic simulations and flow analyses of the chemical adsorption with varying gas flow rates were also executed.

• Composites Research
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• v.37 no.1
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• pp.7-14
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• 2024

Due to the structural characteristic of a double roller, the double roller can have various deformation behaviors depending on a gap block design, even if dimensions and loading conditions for the double roller are the same. Based on this feature, we propose a strategy for designing the gap block of the carbon-fiber reinforced plastic (CFRP) double roller to minimize defects (e.g., sagging and wrinkling), which can be raised during the product conveying process, with the pursue of the lightweight design. In the suggested strategy, analysis cases are first selected by considering main design parameters and engineering tolerances of the gap block, and then deformation behaviors of these selected cases are extracted using the finite element method (FEM). Here, to obtain the optimal gap block parameters that satisfy the purpose of this study, deformation deviations in the contact area are calculated and compared using the extracted deformation behaviors. Note that the contact area in this work is located between the product and the roller. As a result, through the design method of the gap block proposed in this work, it is possible to construct the CFRP double roller that can significantly decrease the defects without changing the overall sizes of the roller. A detailed method is suggested herein, and the results are evaluated in a numerical way.