• 한국수산과학회지
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• 제29권6호
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• pp.770-778
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• 1996

A rapid and economical method of simultaneous extraction of DNA and RNA from seaweeds has been developed by the use of lithium chloride. Lithium chloride facilitates the softening of cell walls resulting in a decrease in both compressive and tensile modulus of elasticity. The DNA was characterized by high molecular weight larger than 27 kb and a relative lack of carbohydrate and protein contamination. The DNA and RNA extracted by the method from many seaweeds were of sufficient quality to be used as a template for per amplification with a plant intergenic gene primer set, for RAPD analysis with arbitrary primers, and for differential display with arbitrary primers in the morphologically distinct regions of the matured Porphyra thallus. The cDNA polymorphism indicated that the reproductive tissue types (male, female, patch) had a relatively high degree of similarity; the vegetative tissue types (dividing, non-dividing) also showed a similar pattern with respect to each other. Holdfast tissue had very low similarity with the other tissues, but appeared most similar to vegetative non-dividing tissue type.

• Applied Microscopy
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• 제44권2호
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• pp.41-46
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• 2014

In this study, the vegetative and reproductive morphology and anatomy of two Hawaiian endemic Portulaca species were examined. Specifically, P. molokiniensis and P. sclerocarpa were compared to closely related species in the genus. The comparisons were both qualitative and quantitative, using characteristics of leaves, stems, roots, and fruits. Tissue organizations of vegetative and reproductive parts of the plants were assessed using microtechnique procedures, statistical analysis, and scanning electron microscopy. The most notable features of these two species were (1) the size and frequency of stomata in P. molokiniensis, and (2) the large number of sclerenchymatous cell layers in the thickest fruit walls of P. sclerocarpa. These findings may imply that stomata development in P. molokiniensis and thick fruit wall development in P. sclerocarpa are evolved features of survival. In particular, the development of thickened walls in indehiscent fruits likely has evolutionary implications of ecological tolerance for better adaptation.

• 식물조직배양학회지
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• 제27권5호
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• pp.359-366
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• 2000

Epigenetics represents a chromatin-mediated transcriptional repression which plays a control role in both animal and plant development. A number of different mechanisms have been described to be involved in the formation of chromatin structure: especially DNA methylation, nucleosomal histone modification, DNA replication timing, and binding of chromatin remodelling proteins. Epigenetic phenomena include genomic imprinting, dosage compensation of X-chromosome linked genes, mutual allelic interactions, paramutation, transvection, silencing of invasive DNA sequences, etc. They are often unstable and inherited in a non-Mendelian way. A number of epigenetic defects has been preferentially described in floral development. Here, epigenetic phenomena in model angiosperm plants and their corresponding mechanisms are reviewed.

• 한국양식학회지
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• 제14권2호
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• pp.73-79
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• 2001

Testicular development of the male African lungfish, Protopterus annectens (Owen) was investigated histologically. The testis was found to be an elongated structure that possessed two distinct portions: an anterior spermatogenic part that was made up of a system of testicular tubules and a posterior vesicular part that invaded the kidney tissue. Spermatogenesis can be divided into five stages; primary spermatogonia, secondary spermatogonia, spermatocyte, spermatids and spermatozoa. Based on the gonadosomatic index (GSI) and histological changes observed, the reproductive cycle can be divided onto four distinct stages: resting and quiescent (December to February), growing (March to June) ripe and spent (July to August) and postspawning (September to November). The GSI was the maximum on July when reproductive cells were mature, ripe and ready for spawning; and the minimum in August after fish spawned.

• 대한수의학회지
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• 제62권2호
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• pp.11.1-11.4
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• 2022

A captive female capybara (Hydrochoerus hydrochaeris) of unknown age discharged a bloody mass from the vaginal region. A histopathology examination revealed the mass to be a reproductive leiomyosarcoma, and an ovariohysterectomy was performed. The histopathology examination confirmed that the excised tissue was a uterine leiomyosarcoma. The purpose of this report is to describe clinical history and histopathological diagnosis of leiomyosarcoma in capybaras. This report is novel because it describes the first diagnosis of uterine leiomyosarcoma in a capybara. Since clinical data about capybaras are rare, this case report will help to diagnosis and treat reproductive diseases of this species.

• Clinical and Experimental Reproductive Medicine
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• 제49권3호
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• pp.168-174
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• 2022

Refractory thin endometrium and recurrent implantation failure are among the most challenging infertility-related factors hindering successful pregnancy. Several adjuvant therapies have been investigated to increase endometrial thickness and the pregnancy rate, but the treatment effect is still minimal, and for many patients, these treatment methods can be quite costly and difficult to approach. Platelet-rich plasma (PRP) is an autologous concentration of platelets in plasma and has recently been elucidated as a better treatment option for these patients. PRP is rich in cytokines and growth factors, which are suggested to exert a regenerative effect at the level of the injured tissue. Another advantage of PRP is that it is easily obtained from the patient's own blood. We aimed to review the recent findings of PRP therapy used for patients with refractory thin endometrium and recurrent implantation failure.

• Reproductive and Developmental Biology
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• 제36권3호
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• pp.219-223
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• 2012

The present study was performed to identify the relationship between plasminogen activator (PA) and Heat Shock Protein-90 (HSP-90) in porcine uterus tissues during the estrous cycle. Porcine uterus tissues were obtained from preovulatory (Pre-Ov), post-ovulatory (Post-Ov) and early to mid-luteal (Early-mid L) stages. The protein was extracted from uterus tissue by using M-PER Mammalian Protein Extraction Reagent. Proteins were refined by RIPA Buffer and quantified by BCA methods. As results, t-PA expression was significantly (p<0.05) higher from pre-ovulatory(Epithelium tissue: $29,067{\mu}g/{\mu}l$, Myometrium tissue: $30,797{\mu}g/{\mu}l$) compared to the post-ovulatory stage(Epithelium tissue: $54,357{\mu}g/{\mu}l$, Myometrium tissue: $53,270{\mu}g/{\mu}l$) and early to mid-luteal stage(Epithelium tissue: $42,380{\mu}g/{\mu}l$, Myometrium tissue: $43,139{\mu}g/{\mu}l$). On the other hand, the uPA expression indicated higher from early to mid-luteal stage (Epithelium tissue: $0.02198{\mu}g/{\mu}l$, Myometrium tissue: $0.02412{\mu}g/{\mu}l$) than pre-ovulatory stage (Epithelium tissue: $0.01577{\mu}g/{\mu}l$, Myometrium tissue: $0.01531{\mu}g/{\mu}l$) and post-ovulatory stage(Epithelium tissue: $0.01414{\mu}g/{\mu}l$, Myometrium tissue: $0.01429{\mu}g/{\mu}l$). However, expression of u-PA did not differ from each estrous cycle in the epithelium tissue and myometrium tissue(p<0.05). Expression of HSP-90 was differ t-PA and u-PA from pre-ovulatory in Epithelium tissue($25,423{\mu}g/{\mu}l$) and early to mid-luteal stage in epithelium tissue($177,922{\mu}g/{\mu}l$) and myometrium tissue($26,664{\mu}g/{\mu}l$). These results suggest that HSP-90 and u-PA were related with change of uterus cycle according to the reformation of the tissues in porcine uterus.

• Clinical and Experimental Reproductive Medicine
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• 제28권3호
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• pp.183-190
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• 2001

Objective: Recently, microdissection of tissue sections has been used increasingly for the isolation of morphologically identified homogeneous cell populations, thus overcoming the obstacle of tissue complexity for the analysis cell-specific expression of macromolecules. The aim of the present study was to establish the minimal conditions required for the RNA extraction and amplification from the cells captured by the laser captured microdissection. Methods : Mouse ovaries were fixed and cut into serial sections (7 im thickness). Oocytes were captured by laser captured microdissection (LCM) method by using PixCell $II^{TM}$ system. The frozen sections were fixed in 70% ethanol and stained with hematoxylin and eosin, while the paraffin sections were stained with Multiple stain. Sections were dehydrated in graded alcohols followed by xylene and air-dried for 20 min prior to LCM. All reactions were performed in ribonuclease free solutions to prevent RNA degradation. After LCM, total RNA extraction from the captured oocytes was performed using the guanidinium isothiocyanate (GITC) solution, and subsequently evaluated by reverse transcriptase-polymerase chain reaction (RT-PCR) for glyceraldehyde-3-phosphate-dehydrogenase (GAPDH). Results: With the frozen sections, detection of the GAPDH mRNA expression in the number of captured 25 oocytes were not repeatable, but the expression was always detectable from 50 oocytes. With 25 oocytes, at least 27 PCR cycles were required, whereas with 50 oocytes, 21 cycles were enough to detect GA PDH expression. Amount of the primary cDNA required for RT-PCR was reduced down to at least 0.25 $\grave{i}$ l with 50 oocytes, thus the resting 19.75 il cDNA can be used for the testing other interested gene expression. Tissue-to-slide, tissue-to-tissue forces were very high in the paraffin sections, thus the greater number of cell procurement was required than the frozen sections. Conclusion: We have described a method for analyzing gene expression at the RNA level with the homogeneously microdissected cells from the small amount of tissues with complexity. We found that LCM coupled with RT-PCR could detect housekeeping gene expression in 50 oocytes captured. This technique can be easily applied for the study of gene expression with the small amount of tissues.

• 생태와환경
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• 제46권3호
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• pp.395-408
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• 2013

환경상태는 생물이 적합도 (번식성공 또는 생존율)를 극대화하기 위해서 초기생활사의 변화를 초래할 수 있다. 본 연구에서는 온도변화와 온도에 따른 수상레저활동 인구변화가 어류의 초기 생활사 특성, 즉 체세포 성장(성장속도), 번식세포 (생식소) 발달 그리고 누적스트레스의 회복과정과 어떠한 관계가 있는지를 동적상태의존모델을 이용하여 분석하였다. 우선 어류의 초기 생활사 특성이 취식행동에 영향을 받는다고 가정하였고, 이러한 관계를 고려하여 어류의 일반 생활사 모델을 개발하였다. 모델은 성장속도와 번식세포(생식소)의 발달이 온도가 상승함에 (단, 성장속도를 감소시키는 임계온도보다는 낮은) 따라 빨라졌으며, 또한 체내에 누적되는 스트레스도 함께 증가하였다. 흥미롭게도 온도가 높을 때에는 수상레저활동 인구의 증가는 성장속도와 생식소의 발달을 느리게 했지만, 스트레스의 누적은 가속화시켰다. 그러나 온도가 낮을 때에는 초기 생활사에 대한 수상레저활동 인구의 영향이 상대적으로 낮았다. 또한 최적취식행동은 높은 온도에서는 수상레저활동 인구의 변화에 관계없이 항상 높았지만, 낮은 온도에서는 수상레저활동 인구가 증가할수록 급격히 감소하였다. 초기성장기간 동안의 생존율은 온도가 낮아지고 수상레저활동 인구가 적을 때에는 취식행동이나 인간 활동에 따른 어류의 사망률 증감이 생존률 변이에 영향을 주었다. 반대로 온도가 높아지고 수상레저활동 인구가 많을 때의 생존율은 취식행동이나 사망률에 관계없이 항상 낮았다. 끝으로 본 연구를 통해 기후변화와 수상레저활동 인구변화와 관련된 어류의 초기 생활사를 수생태계 보전전략이나 건강성 평가분석에 포함시키는 것은 분석의 정확성과 정밀성을 향상시킬 수 있을 것이라 사료된다.

• 한국임상수의학회지
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• 제11권2호
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• pp.573-578
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• 1994

A lymphosarcoma was diagnosed in a 4-year-old female Formosan sika deer presented with persistent reproductive failure, anorexia, depression and diarrhea. Characteristic pathological findings were infiltration of neoplastic lymphoid cells and cancer emboli in the lymph nodes, heart, lung, kidney, urinary bladder, ovary, uterus and peritoneal fat tissue.