• Title/Summary/Keyword: representative frame

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NDP Kinases Suppressed Bax-Dependent Apoptosis in Yeast System

  • K. C. Hwang;D. W. Ok;D. N. Kwon;H. K. Shin;Kim, J. H.
    • Proceedings of the KSAR Conference
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    • 2001.03a
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    • pp.52-52
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    • 2001
  • Many nucleoside diphosphate (NDP) kinases are ubiquitous enzymes responsible for the exchange of ${\gamma}$-phosphates between tri- and diphosphonucleosides. The catalytic Many nucleoside diphosphate (NDP) kinases are ubiquitous enzymes responsible for the exchange of ${\gamma}$-phosphates between tri- and diphosphonucleosides. The catalytic reaction follows a ping-pong mechanism in which the enzyme is transiently phosphorylated on a histidine residue conserved in all nucleoside diphosphate kinases. Beside their role in nucleotide synthesis, these enzymes present additional functions, possibly independent of catalysis, in processes such as differentiation, cell growth, tumor progression, metastasis and development. To clone murine nm23-M5, several expressed sequence tags (ESTs) of the GenBank data base, selected according to their homology to nm23-H5 cDNA, reconstituted a complete open reading frame (GenBank AF222750). To test whether murine NDPKs (1, 2, 3, 4, 5, and 6) can inhibit Bax-mediated toxicity in yeast, co-transformation was performed respectively. The yeast S.cerevisiae was transformed with a copy expression plasmid containing the histidine selection marker and expressing murine Bax under the control of a galactose-inducible promoter. Several clones were selected and found to be growth inhibited when Bax expression was induced with galactose. A representative clone was transformed again with a copy expression plasmid containing the tryptophane selection marker and expressing either murine Bcl-xL or NDPK under the control of a galactose-inducible promoter. Several subclones of the double-transformants were selected and characterized. The ability of Bcl-xL and NDPKs to suppress Bax-mediated toxicity was determined by growing yeast cells overnight in galactose media and spot-testing on galactose plates starting with an equal number of yeast cells as determined by taking the OD$_{600}$. Ten-fold serial dilutions were used in the spot-test. Plates were grown at 3$0^{\circ}C$ for 2-3 days. All murine NDPKs suppressed Bax dependent apoptosis. Futher study will be peformed whether Bax-toxicity inhibition was caused by NDP kinase activity or additional function.n.

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Review in terms of the earthen wall stamped technique in the Three Kingdoms period (삼국시대 토성 판축기법 용어 검토)

  • SHIN Heekweon
    • Korean Journal of Heritage: History & Science
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    • v.55 no.4
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    • pp.38-53
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    • 2022
  • In this article, I have summarized and reviewed the concepts and terms surrounding the stamped construction technique, focusing on earthen walls in the Three Kingdoms period. This is because confusion is caused by defining the nature of the earthen walls by using various concepts and terms for each researcher regarding the substance and construction method of the earthen walls. The stamped earth method is a representative ancient civil engineering or construction technique in which a frame is made of plates to form a fortress wall, a fence, and the base of a building, and then soil or sand is poured into it layer by layer and then stamped with a bat to make it solid. Therefore, in order to prove that the earthen wall was built by the stamped earth method, evidence such as a narrow plate, a column for fixing it, long horizontal and vertical wood pieces to support the narrow plate, and traces of pounding the soil must be detected. However, in Korea, there are very few cases where such evidence has been fully excavated, so it is necessary to agree on how strictly the standards for the stamped earth method will be applied. The terms related to the stamped technique mobilized for the construction of the earthen walls were explained with actual examples by dividing the terms related to the concept into terms related to the principle and unit of the stamped plate, and the specific stamped technology. In particular, in Pungnabtoseong Earthen wall, a variety of typical and diverse methods of building the ancient stamped earthen wall were identified so that decisive data could be secured to understand the principles and techniques of the stamped earthen wall. In the future, a more general understanding of the stamped technique will be possible only when more evidence related to it is found in relics other than Pungnabtoseong Earthen wall.

Automated Finite Element Analyses for Structural Integrated Systems (통합 구조 시스템의 유한요소해석 자동화)

  • Chongyul Yoon
    • Journal of the Computational Structural Engineering Institute of Korea
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    • v.37 no.1
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    • pp.49-56
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    • 2024
  • An automated dynamic structural analysis module stands as a crucial element within a structural integrated mitigation system. This module must deliver prompt real-time responses to enable timely actions, such as evacuation or warnings, in response to the severity posed by the structural system. The finite element method, a widely adopted approximate structural analysis approach globally, owes its popularity in part to its user-friendly nature. However, the computational efficiency and accuracy of results depend on the user-provided finite element mesh, with the number of elements and their quality playing pivotal roles. This paper introduces a computationally efficient adaptive mesh generation scheme that optimally combines the h-method of node movement and the r-method of element division for mesh refinement. Adaptive mesh generation schemes automatically create finite element meshes, and in this case, representative strain values for a given mesh are employed for error estimates. When applied to dynamic problems analyzed in the time domain, meshes need to be modified at each time step, considering a few hundred or thousand steps. The algorithm's specifics are demonstrated through a standard cantilever beam example subjected to a concentrated load at the free end. Additionally, a portal frame example showcases the generation of various robust meshes. These examples illustrate the adaptive algorithm's capability to produce robust meshes, ensuring reasonable accuracy and efficient computing time. Moreover, the study highlights the potential for the scheme's effective application in complex structural dynamic problems, such as those subjected to seismic or erratic wind loads. It also emphasizes its suitability for general nonlinear analysis problems, establishing the versatility and reliability of the proposed adaptive mesh generation scheme.

Study on the Economic Co-operation action by analyzing the North Korea's Military Negotiations - Focusing on Inter-Korean Military Talks and working level talks about Gaeseong industrial complex - (북한 협상모델 분석을 통한 경제협력 실천방안 연구 - 남북 군사협상 및 개성공단 실무회담 사례를 중심으로-)

  • Lee, Sung-Choon
    • International Commerce and Information Review
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    • v.15 no.3
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    • pp.353-384
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    • 2013
  • When it comes to the current inter-Korean relationship, the two Koreas are in the step of core practical negotiation on exchanges and cooperation away from deadlock so far. It is expected that they will have more and frequent meetings in the future. Therefore, now is the time the South Korea needed to come up with systematic countermeasures because there is nothing more important and giving more impact on our society than the matter of North Korea. As the purpose of social science lies with the explanation and prediction of the social phenomena in the society, it is considered to be meaningful to analyze the representative military negotiations such as the defense ministry-level talks, general-level talks, and working-level talks between the two Koreas where the participants from South Korea consisting of the military representatives discussed with their counterparts of North Korea since the signing of the armistice in Korea on July 27, 1953. This study analyzes and evaluates the behaviors of North Korea's military negotiations with the South Korea in the Kim Jong-il era on the overall basis. In particular, the research tries to prove that the behaviors of military negotiations under Kim Jeong-il regime were made in the frame of the negotiation model by analyzing many negotiations presented in 'With Century', Kim Il Sung's Memoirs under his anti-Japan-guerilla era and suggesting the analysis frame of anti-Japan-guerilla style negotiation model. Based on the results of this proof, the study looks at carefully the specific characteristics of anti-Japan guerrilla-type negotiation.

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Evaluation of Robustness of Deep Learning-Based Object Detection Models for Invertebrate Grazers Detection and Monitoring (조식동물 탐지 및 모니터링을 위한 딥러닝 기반 객체 탐지 모델의 강인성 평가)

  • Suho Bak;Heung-Min Kim;Tak-Young Kim;Jae-Young Lim;Seon Woong Jang
    • Korean Journal of Remote Sensing
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    • v.39 no.3
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    • pp.297-309
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    • 2023
  • The degradation of coastal ecosystems and fishery environments is accelerating due to the recent phenomenon of invertebrate grazers. To effectively monitor and implement preventive measures for this phenomenon, the adoption of remote sensing-based monitoring technology for extensive maritime areas is imperative. In this study, we compared and analyzed the robustness of deep learning-based object detection modelsfor detecting and monitoring invertebrate grazersfrom underwater videos. We constructed an image dataset targeting seven representative species of invertebrate grazers in the coastal waters of South Korea and trained deep learning-based object detection models, You Only Look Once (YOLO)v7 and YOLOv8, using this dataset. We evaluated the detection performance and speed of a total of six YOLO models (YOLOv7, YOLOv7x, YOLOv8s, YOLOv8m, YOLOv8l, YOLOv8x) and conducted robustness evaluations considering various image distortions that may occur during underwater filming. The evaluation results showed that the YOLOv8 models demonstrated higher detection speed (approximately 71 to 141 FPS [frame per second]) compared to the number of parameters. In terms of detection performance, the YOLOv8 models (mean average precision [mAP] 0.848 to 0.882) exhibited better performance than the YOLOv7 models (mAP 0.847 to 0.850). Regarding model robustness, it was observed that the YOLOv7 models were more robust to shape distortions, while the YOLOv8 models were relatively more robust to color distortions. Therefore, considering that shape distortions occur less frequently in underwater video recordings while color distortions are more frequent in coastal areas, it can be concluded that utilizing YOLOv8 models is a valid choice for invertebrate grazer detection and monitoring in coastal waters.

A Kinematic Analysis of Uchi-mata(inner thigh reaping throw) by Kumi-kata types in Judo (유도 맞잡기 타입에 따른 허벅다리걸기의 Kinematic 분석[I])

  • Kim, Eui-Hwan;Cho, Dong-Hee;Kwon, Moon-Seok
    • Korean Journal of Applied Biomechanics
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    • v.12 no.1
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    • pp.63-87
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    • 2002
  • The purpose of this study was to analyze the kinematic variables when Uchi-mata(inner thigh reaping throw) performing by Kumi-kata(engagement position, basic hold) types A, B(A: grasping part-behind neck lapel, B: chest lapel) in Judo with three dimensional analysis technique DLT method by videography. The subjects were four male judokas who have been training in Yong-In University(YIU), on Korean Representative level and Uchi-mata is their tokui-nage(favorite technique), the throwing form was filmed on two S-VHS 16mm video camera( 30frame/sec. Panasonic). Kinematic variables were temporal, posture, and COG. The data collection was performing by Uchi-mata. Six good trials were collected for each condition (type A, B) among over 10 trials. The mean values and the standard deviation for each variable were obtained and used as basic factors for examining characteristics of Uchi-mata by Kumi-kata types. The results of this analysis were as follows : 1) Temporal variables The total time elapsed(TE) by Uchi-mata of types A, B were 1.45, 1.56 sec. respectively. Types A shorter than B. 2) Posture variables In performing of Uchi-mata, the range of flexion in type A, left elbow was $45^{\circ}$ and B was $89^{\circ}$ from Event 2(E2) to Event 6(E6). Type A and B were quite different in right elbow angle in Event1(E1). Left shoulder angle of type A was extended and type B was flexed in E4. Both types right shoulder angles were showed similar pattern. Also both hip angles(right/left) were showed similar pattern. When type A performed Uchi-mata the knee-angle of supporting foot showed $142^{\circ}$in the 1st stage of kake phase[KP], and extended to $147^{\circ}$in the 2nd stage of KP. And the foot-ankle angle of supporting foot showed $83^{\circ}$in the 1st stage of KP, and extended to $86^{\circ}$in the 2nd stage of KP. moreover, The knee angle of attacking foot showed $126^{\circ}$in the 1st stage of KP, and extended to $132^{\circ}$in the 2nd stage of KP, and the foot-ankle angle of attacking foot showed $106^{\circ}$in the 1st stage of KP, and extended to $121^{\circ}$in the 2nd stage of KP. When type B performed Uchi-mata the knee-angle of supporting foot showed $144^{\circ}$in the 1st stage of KP, and extended to $154^{\circ}$in the 2nd stage of KP. And the foot-ankle angle of supporting foot showed $83^{\circ}$in the 1st stage of KP, and extended to $92^{\circ}$in the 2nd stage of KP. moreover, The knee angle of attacking foot showed $132^{\circ}$in the 1st stage of KP, and extended to $140^{\circ}$in the 2nd stage of KP, and the foot-ankle angle of attacking foot showed $103^{\circ}$in the 1st stage of KP, and extended to $115^{\circ}$in the 2nd stage of KP. During Uchi-mata performing, type A showed pulling pattern and type B showed lift-pulling pattern. As Kumi-kata types, it were different to upper body(elbow, shoulder angle), but mostly similar to lower body(hip, knee, ankle angle) on both types. 3) C. O. G. variables When the subjects performed Uchi-mata, COG of type A, B up and down in vertical aspect was 71cm, 73.8cm in height from the foot in the 2nd stage of KP. As Kumi-kata types, it were different on medial-lateral direction aspect but weren't different in Kuzushi phase on vertical direction aspect.

Characteristic Analysis of the Changes of Landscape Architecture Construction Costs in Time-series - Focused on the Cases of Works of Public Institution's at Capital Area - (아파트단지 조경 공사비의 공종별 경년변화 특성 분석 - 공공기관 시행의 수도권 아파트단지를 중심으로 -)

  • Park, Sang-Jin;Cho, Se-Hwan
    • Journal of the Korean Institute of Landscape Architecture
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    • v.43 no.6
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    • pp.150-159
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    • 2015
  • This study was conducted for the purpose of presenting basic data for calculating a more reasonable landscaping project cost in the future through the construction cost calculation ratio of scenic planting and facilities and their time series trend analysis targeting 'J' corporation, a representative Korean public institution that creates apartment complexes. This study targeted scenic planting and the facility construction costs of 37 apartment complexes in the capital area from 2004 to 2012, using statistical analysis methods such as technical analysis, correlation analysis, and regression analysis to analyze the characteristics of the time series change. The conclusion was drawn as follows. First, breaking down the cost of overall landscaping projects to scenic planting and facility construction, the ratio of the average cost of scenic planting to that of facility construction showed 56.1% to 43.9% from 2004 to 2012. Second, the costs of planting construction and facility construction both showed a fluctuation range of about ${\pm}3%$, implying relatively steady costs considering the inflation rate. Third, the landscape construction cost for each type of construction resulted in a fluctuation range from minimum ${\pm}3%$(exercise facility) to maximum ${\pm}5%$(packing facility), reflecting that among the landscaping projects, the facility construction costs tended to show relatively large fluctuations in accordance with the change of time series. Fourth, the comprehensive indication of the ratio of landscaping project costs by time series and landscaping construction type implies that the regional characteristics and positional condition of the apartment complexes were not reflected sufficiently. Fifth, the high level of correlation of landscaping construction types and landscaping construction elements imply that the entire construction costs were controlled through partial adjustment of cost components within the overall frame of construction cost. These results reveal the problems of standardized landscaping cost irrespective of user satisfaction or environmental traits such as the conditions of the apartment complexes.

A Study on Curriculum Improvement of the Korea Army Nursing Academy (국군간호사관학교 교육과정 개선을 위한 기초 연구)

  • 고자경
    • Journal of Korean Academy of Nursing
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    • v.13 no.2
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    • pp.22-43
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    • 1983
  • 1. Need for and Purpose of the Study. There is an increasing demand for curriculum improvement of the Korean Army Nursing Academy (KANA), since it was upgraded into 4-year institution of higher learning from 3-year one. In particular, it is strongly advocated that the KANA needs the outside expertise for its curriculum improvement-namely not only from the internal military view of points but also from the viewpoints of professional educational society, In line with such a necessity for the study, this study was aimed at 1) analyzing the current actual practices of KANA'S curriculum, 2) investigating the desired practices of KANA'S curriculum, and 3) identifying the discrepancy between the actual and desired practices of curriculum. 2. Problems for the Study This study had 4 problems to be answeared as follows: 1) What are the actual curriculum practices of KANA? 2) What are the desired curriculum practices of KANA? 3) How are the extents of perception of actual and desired curriculum different in four groups (student, faculty & administrator, nurse, and medical doctor in militay hospital) ? 4) What are the restraining forces that impede the change from actual to desired curriculum practices? 5) What are the relationships of curriculum component,』 in actual and desired curriculum practices? 3. Methods and Procedures This study was conducted by means of document analysis in addition to literature review and by means of needs assessment questionnaire which was developed by the researcher. The questionnaire included 62 statments with 7 questions for demographic data collection. The needs assessment questionnaire was managed to a total of 243 subjects (100 students, 46 faculty & administrators, 55 nurses, and 42 medical doctors), The collected data were treated using SPSS computer system so as to calculate mean scores, standard deviations, and correlation coefficients. The significance test was made through t-test and one-way ANOVA. The statistical significance level was set at both .05 and .01 level. 4. Major findings The major findings in this study are as follows: 1) The score of desired practices was significantly greater than that of actual practices, representing a strong need for curriculum betterment. 2) There were significant differences in the perceptions of actual practices as well as desired practices among four groups (student, faculty & administrater, nurse, and medical doctor). 3) The most frequently selected restraining forces were army's inherent character, economical limitation, and educational expertise limitations. 4) Such variables as sex, position attachment to the KANA and grade made a statistically significant effect on the perception of desired curriculum practice, while the variables like marrige, position, and military class made it on the perception of actual curriculum practice. 5) The coefficients among the curriculum components were lower in perception of the actual curriculum practices than those in the desired practices. 5. Conclusions The conclusions based on the major findings of this study are as follows: 1) The current curriculum development procedure of the KANA is not consistent with the theoretical frame of systematic development sarategy of curriculum. 2) There are wide conflicts among the groups who are supposed to participate in curriculnm development, concerning the actual and desired practices of KANN'S curriculum. 3) A great deal of need for curriculum improvement for the KANA is clearly felt, and in particular, in the process of teaching and learning. 4) Each component of curriculum is not intergrated into a whole development procedure, being segregated each other. 5) For better curriculum improvement, such restraining forces as financial and professional limitations should be eliminated. 6. Recommendations 1) For Further Research a. There is a need to replicate this study after in-depth statistical analysis of each item of need assessment questionnaire, and with more representative subjects. b. A study should be conducted which. has its focus on the analysis of restraining forces for the change from actual to desired curriculum practices of the KANA. 2) For KANA'S Curriculum Improvement a. There is a need to promote the professional expertise of the participants in curriculum development and the communication among them. b. It is desirable to establish an institution or section of administration, which is soley in charge of curriculum development. c. To better develop KANA's curriculum not only faculty and administrators but also students should be encouraged to participate in development process, while the military medical doctors' participation should be carefully considered.

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Design and Implementation of Content-based Video Database using an Integrated Video Indexing Method (통합된 비디오 인덱싱 방법을 이용한 내용기반 비디오 데이타베이스의 설계 및 구현)

  • Lee, Tae-Dong;Kim, Min-Koo
    • Journal of KIISE:Computing Practices and Letters
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    • v.7 no.6
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    • pp.661-683
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    • 2001
  • There is a rapid increase in the use of digital video information in recent years, it becomes more important to manage video databases efficiently. The development of high speed data network and digital techniques has emerged new multimedia applications such as internet broadcasting, Video On Demand(VOD) combined with video data processing and computer. Video database should be construct for searching fast, efficient video be extract the accurate feature information of video with more massive and more complex characteristics. Video database are essential differences between video databases and traditional databases. These differences lead to interesting new issues in searching of video, data modeling. So, cause us to consider new generation method of database, efficient retrieval method of video. In this paper, We propose the construction and generation method of the video database based on contents which is able to accumulate the meaningful structure of video and the prior production information. And by the proposed the construction and generation method of the video database implemented the video database which can produce the new contents for the internet broadcasting centralized on the video database. For this production, We proposed the video indexing method which integrates the annotation-based retrieval and the content-based retrieval in order to extract and retrieval the feature information of the video data using the relationship between the meaningful structure and the prior production information on the process of the video parsing and extracting the representative key frame. We can improve the performance of the video contents retrieval, because the integrated video indexing method is using the content-based metadata type represented in the low level of video and the annotation-based metadata type impressed in the high level which is difficult to extract the feature information of the video at he same time.

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A Study on the Imitation and Transformation of Gugok-Wonlim Culture through Management of the Myungam Jeong Sik's Muyi-Gugok in Sancheong (명암(明庵) 정식(鄭拭)의 산청 무이구곡(武夷九曲) 원림경영을 통해 본 구곡문화의 모방과 변용)

  • Rho, Jae-Hyun
    • Journal of the Korean Institute of Traditional Landscape Architecture
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    • v.33 no.3
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    • pp.84-94
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    • 2015
  • This study is to examine how the admiration for Chutzu(朱子)'s achievement while he was preaching his policies after renouncing the world in Wuyi-Gugok(武夷九曲), Mt. Wuyi and Joseon-style transformation culture were unfolded and developed through Gugok management cases such as position, name, poetic diction, Jungsa(精舍) architecture and engraving of Muyi-Gugok set in Mt. Gugok, Snacheong. The results were as follows: Myungam(明庵) Jeong Sik(鄭拭, 1683~1746)'s Muyi-Gugok, which consists of Suhongkyo(垂虹橋, gok 1) - Oknyeobong(玉女峰) - Nhongwhaldam(弄月潭) - Nacwhadam(落花潭) - Daeeunbyeong(大隱屛) - Gwangpyungryea(光風瀨) - Jaewhaldae(霽月臺) - Gorooam(鼓樓巖) - Wharyongpok(臥龍瀑, gok 9) is the representative case where Chutzu's Wuyi-Gugok was exactly copied and fulfilled to the Joseon Dynasty. In a large frame, Gugok Wonlim culture, Myungam's Muyi-Gugok management has a will of succession of Dotong(道統) through admiration for Chutzu in a rigid way. Another name of Mt. Gugok is Mt. Muyi and Gugok's name is Muyi-Gugok and the residence existed between gok 4 and gok 5. In addition, the name of Jeongsa for Gugok management is also 'Muyi Jeongsa(武夷精舍)' and Gugok name and contents of Poetry are also similar and all of these are clear evidence that Myungam tries to copy Chutzu's Wuyi-Gugok to Mt. Gugok. Also, Gugok set before Myungam were located in Mt. Gugok and among them, verified four Gugok names are corresponded to those of Chutzu's Wuyi-Gugok and it tells that conforming behavior as one of admiration ways for Chutzu already arrived at Mt. Gugok before Myungam and this was an oppotunty to widen Mt. Gugok Muyi-Gugok's tradition and horizon. Also, considering that Myungam's gok 6, Gwangpyungryea and gok 7, Jewoldae are names from 'Gwangpungjewol(光風霽月)' Based on Chutzu's poem and they are closely related to Joseon's classical scholar spirit, they are associated with Joseon-style transformation of Chutzu's Muyi-Gugok. Meanwhile, gok 5 'Daeeunbyeong' was transformed to 'Nangaam(爛柯巖)' in gok 5 - "Deoksan-Gugok(德山九曲) of Jooko(竹塢) Ha Beom-Woon(河範運, 1792~1858) and those characters's engravings are handed down. In "Pome of Deoksan Gugok" transformed from Myungam's Muyi-Gugok, respect and admiration for Chutzu is weaken while Ha Beom-Woon admires Nammyeong(南冥) Cho shik(曺植, 1501~1572), a symbolic character of himself's school and from this, a movement to promote partisan unity is identified. After Myungam died, Muyi-Gugok in Mt. Gugok was transformed from a space to succeed Chutzu's Dotonga to one to commemorate the memory of ancient sages, but, it is a typicality case that widen the spectrum of Joseon's Gugok-Wonlim culture through Muyi-Gugok's imitation and transformation.