• Journal of Ginseng Research
• /
• v.26 no.3
• /
• pp.151-158
• /
• 2002

Ginseng (Panax quinquefolius L.) has become one of the most valuable herb crops grown in North America. However, traditional cropping practices are favourable to disease and significant losses due to root disease are common, despite frequent use of fungicides. Seedlots are often contaminated with pathogens, however, little is known about the causes of seed decay and the role of seed pathogens as incitants of root rots. It was shown that both Fusarium spp. and Cylindrocarpon destructans were able to rot seeds and that C. destructans was more virulent than Fusarium spp. on seedling roots. A modified rose bengal agar MRBA) medium (1 g KH$_2$PO$_4$; 0.5 g MgSO$_4$; 50 mg rose bengal; 10 g dextrose; 5 g Bacto peptone; 15 g Bacto agar; 30 mg streptomycin sulfate; 250 mg ampicillin; 10 mg rifampicin; 500mg pentachloronitrobenzene; 500 mg dicloran; and 1 L distilled water) was superior to potato dextrose agar in detecting C. destuctans in diseased roots. Isolation of C. destructans from diseased seedlings arising from seeds sown in replant soil supported the hypothesis that this pathogen is a cause of ginseng replant failure in North America.

• The Plant Pathology Journal
• /
• v.21 no.3
• /
• pp.244-251
• /
• 2005

The induction of growth promotion on numerous crops by rhizobacteria is a well documented phenomenon. In case of tomato (Lycopersicon esculentum), fruit yield is higher in replant soil than that in fresh soil. To investigate what kind of rhizobacterium is involved, microbial community in rhizosphere and on rhizoplane of tomato plants from each soil was analyzed by dilution plating on selective media. Many Gram-negative bacteria and actinomycetes were isolated from tomato in replant soil. One Gram-negative rhizobacterium isolated was identified as Pseudomonas putida based on its biochemical characteristics, fatty acid methyl ester analysis and 16S rDNA sequence. This bacterium designated strain 17 inhibited the growth of Pseudomonas corrugata, and increased growth of tomato seedlings. In addition, its culture filtrate inhibited conidial germination of plant-pathogenic fungi such as Fusarium oxysporum f. sp. radicis-lycopersici, F. oxysporum f. sp. cucumerinum, and Nectria radicicola. Scanning electron microscopy revealed strain 17 colonized and persisted on the epidermal surfaces of tomato radicles and roots. These results suggest that P. putida strain 17 may serve as a biological control agent to suppress multiple soil-borne diseases for tomato plants. Increased microbial populations that suppress deleterious microorganisms including pathogens could be one of the major factors in increased tomato yield in replant soil.

• Archives of Plastic Surgery
• /
• v.38 no.5
• /
• pp.636-641
• /
• 2011

Purpose: There are multiple dependent variables commonly attributed to survival of replanted digits. The ischemia time is thought to be a clinically relevant factor. However, controversy exists as large hand centers have reported successful replant outcomes independent of ischemic time. In this study, we present a single institution experience on the effect of ischemia time on the survival of completely amputated digits. Methods: A retrospective review of a single institution experience was performed. This cohort included all comers who had suffered complete amputation of a digit (Zone 2-4) and underwent replantation from 2003 to 2009. Demographic information as well as injury mechanism, ischemic time, and replantation outcome were recorded for each patient. Chi-square was used to analyze the result. Results: Mean age was 35.5 years old (2-69). Mean replantation survival was 89.5% (37/317). Survival rates were 94, 88, and 88% in respective groups of 0~6, 6~12, of > 12 hours of ischemia time. In chi-square analysis, there was no difference with $p$ value of 0.257. No other independent patient factors showed statistically significant relationship to replant survival rate. In the group with longest ischemia time (12~18 hours) replant survival rate was 88% (37/42). Conclusion: Prolonged ischemia time is commonly believed to be a contributing factor for replant survival. However, our experience has shown that survival rate is uniform up to 18 hours of ischemia.

• Current Research on Agriculture and Life Sciences
• /
• v.11
• /
• pp.91-99
• /
• 1993

In order to clarify the possibility of occurrence of apple replant disease and the severity of it in Kyungbuk area, 23 apple orchard soils older than 20 years were collected and growth response of apple seedlings in the soil was tested by soil fumigation. Some factors concerned with apple replant disease in some cases were also checked. The results were as follows. 1. Plant height of apple seedlings was significantly increased in 6 apple orchards and up to 38% in a most conspicuous case by soil fumigation. 2. Fresh weight of aboveground part(except leaves), underground part and whole plant(except leaves) of apple seedlings was significantly increased in 10,4 and 9 apple orchards, respectively by soil fumigation. The response of soil fumigation was pronounced in fresh weight of aboveground part of apple seedlings, more than 50% increase in 5 apple orchards. 3. The effects of available copper and arsenic content in soil and soil nematodes population on the plant height and fresh weight of apple seedlings were not recognized as important factors causing apple replant disease. 4. Effect of soil PH on the occurrence of apple replant disease was not recognized.

• Proceedings of the Korean Society of Crop Science Conference
• /
• 2020.06a
• /
• pp.101-101
• /
• 2020

• Restorative Dentistry and Endodontics
• /
• v.24 no.2
• /
• pp.426-429
• /
• 1999

When the tooth avulsion occur in accidents the drying damage to the periodontal ligament has extremely detrimental effects on healing. Pulp necrosis always occurs after an avulsion injury, but revascularization can only take place in teeth with immature apexes. Therefore complications after avulsion injuries are common, and treatment must be carried out in a timely and correct fashion to prevent or limit these complications. Every effort should be made to replant the tooth within the first 15 to 20 minutes. If doubt exists that the tooth can be replanted adequately, the tooth should quickly be stored in an appropriate medium until the patient can get to the dental office for replantation. A complication of inflammatory root resorption is occurred by bacterial infection of periodontal ligament and dental pulp. Therefore aseptic endodontic treatment must be carried out in a timely and systemic antibiotics given at the time of replantation and before endodontic treatment are effective in preventing bacterial invasion. Further studies are needed to establish the clinical importance of preparation of the socket and root.

• Korean Journal of Medicinal Crop Science
• /
• v.26 no.3
• /
• pp.248-253
• /
• 2018

Background: Dazomet are widely used as soil fumigant to solve soilborne problems, and the degradation intermediates are toxic to nematodes, fungi, bacteria, insects and weeds. Methods and Results: The effects of cultivation of green manure crop, maize before and after soil fumigation on the control of ginseng root rot disease were compared using soil where 6-years-old ginseng was harvested. Fumigant (dazomet) were used for soil fumigation in May and September, respectively. Maize was grown for soil management before and after soil fumigation. After May fumigation, the sowing date of maize was delayed by 15 days and thus its dry weight was decreased significantly. Maize cultivation after May fumigation increased pH but decreased EC, $NO_3$, $P_2O_5$, and K significantly. Maize cultivation after May fumigation decreased fungi population and the ratio of fungi and bacteria. Growth of 2-years-old ginseng was improved and the incidence of ginseng root rot was significantly decreased by maize cultivation after May fumigation. After harvesting 2-years-old ginseng, the population of Cylindrocarpon destructans was not different between treatment of May and September, but Fusarium solani showed a significant increase in September fumigation after maize cultivation. Conclusions: Maize cultivation after soil fumigation was effective in inhibiting ginseng root rot by the amendment of mineral composition and microorganism in fumigated soil.

• Korean Journal of Medicinal Crop Science
• /
• v.27 no.4
• /
• pp.284-291
• /
• 2019

Background: Ginseng root rot disease, caused by Cylindrocarpon destructans and Fusarium solani is a major cause of replant failure in continuous cropping ginseng. Methods and Results: To control replant injury in soil infected with C. destructans and F. solani, biosolarization was performed by covering the plot with transparent polyethylene film after adding green manure of maize and sunflower for the summer season. Per 10 a, fresh and dry weight of maize was 10.1 and 2.5 tons, respectively, and that of sunflower was 8.1 tons and 1.2 tons, respectively. Mean maximum temperature at 20 cm depth was $33.2^{\circ}C$, $41.5^{\circ}C$ and $41.8^{\circ}C$ in the control, maize-incorporated and sunflower-incorporated plots, respectively. The elapsed time over $40^{\circ}C$ was 36.4 h in the maize-incorporated plot and 77.3 h in the sunflower-incorporated plot. Biosolarization increased $NO_3$ content in soil, while content of organic matter, Ca, and Mg was decreased. Electrical conductivity, $NO_3$ and $P_2O_5$ in soil significantly increased after two years of biosolarization. The number of spores of C. destructans in soil was significantly decreased by biosolarization, and sunflower treatment was more effective than maize treatment in decreasing the number of spores. Root yield of 3-year-old ginseng was significantly increased by biosolarization, however, there was no significant difference between maize and sunflower treatments. Rate of root rot in 3-year-old ginseng decreased to 16.5% with the incorporation maize and 5.0% with the incorporation of sunflower, while that in control 25.6%. Conclusions: Biosolarization was effective in inhibiting ginseng root rot by decreasing the density of root rot disease and improving soil chemical properties.

• Archives of Reconstructive Microsurgery
• /
• v.22 no.2
• /
• pp.82-85
• /
• 2013

If the replantation on the original position is not possible, the amputated tissue of a hand may be used as a donor for recovering hand functions at other positions. This procedure is termed 'heterodigital replantation'. An 63-year-old male patient who was in press machine accident came to Our Hospital. He had large dorsal soft-tissue defects ($5{\times}3cm$) on his left long finger and complete amputation on his left index finger through the proximal interpharyngeal joint. Replantation was not indicated because crushing injury of index finger was severe. So we decided to use index finger soft tissue as heterodigital free flap for the coverage of the long finger defect. The ulnar digital artery and dorsal subcutaneous vein of the free flap were anastomosed with the radial digital artery and dorsal subcutaneous vein of the long finger. The heterodigital free flap provided satisfactory apperance and functional capability of the long finger. The best way to treat amputation is replantation. But sometimes surgeon confront severely crushed or multi-segmental injured amputee which is not possible to replant. In this situation, reconstructive surgeons should consider heterodigital free flap from amputee as an option.

• Proceedings of the Ginseng society Conference
• /
• 2002.10a
• /
• pp.212-226
• /
• 2002

North American ginseng production may have been maximized in the traditional growing areas in the last decade and further increases may be in woods grown root, for niche markets. The marketplace demands high quality roots. Most problems leading to low quality roots start with the grower and can be avoided. These include poor site selection, inadequate soil drainage, untimely and poorly applied pesticides, and neglect of good sanitary practices. Selection of low lying sites increased the plant damage from frost in Ontario in May 2002. Seeding is still the major method of propagation of ginseng in spite of some success in culturing different parts of the plant. Opportunities exist for shortening the stratification period of North American ginseng seed to allow spring planting. This may reduce disease incidence. Since only one-third of ginseng seed sown ultimately produces plants harvested after 3 years any approach that reduces disease incidence and improves seed germination, seedling emergence and crop stand must be pursued. Disease is the major problem in ginseng cutivation from seed stratification, soil preparation prior to planting, right through to drying of the roots. Replant disease remains as an unresolved problem and needs full characterization and new approaches for control. Much progress has been made in research and related extension activities in disease control although challenges will arise such as with Quintozene and its replacement with Quadris for control of diseases caused by Rhizoctonia. Decreased labor populations and increased associated costs for ginseng production are causing rapid mechanization in every aspect of the ginseng industry. Engineers, machinery dealers, and fabricators, and growers are being challenged to increase efficiency by mechanization.