• 한국식물학회:학술대회논문집
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• 한국식물학회 1995년도 식물학심포지움 식물로부터 유용 2차대사산물의 생산 PRODUCTION OF USEFUL SECONDARY METABOLITES FROM PLANTS
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• pp.67-78
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• 1995

Monoclonal antibodies (MoAbs) are a highly diversified class of proteins with major research and commercial applications such as diagnostics and therapeutics. Currently, the dominant method for producing MoAbs is through the hybridoma technique. However, this technique is slow, tedious, labor intensive, and expensive. The production of MoAbs in cultured transgenic plant cells can offer some advantages over that in the over that in the mammalian systems. The media to cultivate plant cells are well defined and inexpensive. Contamination by bacteria or fungi is easily monitored in plant tissue cultures. Furthermore, these contaminants are usually not potent pathogens to human beings. In our interdisciplinary research efforts, heavy chain monoclonal antibody (HC MAb) was inserted into Ti plasmid vector and transferred into A. tumefaciens for the transformation in tobacco cells. It was found that 76% of the transformants produced HC MAb. The presence of HC MAb in the cell membrane fraction indicated that the signal peptide was functional and efficient. The change of the HC MAb concentration during a batch culture followed a similar trend as dry cell concentration, indicating that the production of HC MAb was growth related. The long-term repeated subcultures of 11 cell lines showed that there was no obvious trend of neither the decrease nor the increase of the productivity with the repeated subcultures.

• 한국구조물진단유지관리공학회 논문집
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• 제14권4호
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• pp.148-153
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• 2010

각종 건설현장에서 구조재료로 사용되고 있는 콘크리트는 레미콘 공장에서 배합되어 레미콘 트럭에 의해 현장까지 운송 타설되는 형태로 공급되고 있다. 그러나 콘크리트가 현장까지 운송되는 동안 교통 체증, 기후 및 기타 여러 영향으로 인해 적정한 슬럼프의 유지가 곤란한 경우를 겪게 된다. 이럴 경우 콘크리트의 적정한 슬럼프 유지를 위해 혼화제를 첨가하게 된다. 그러나 실제 현장에 배달되는 레미콘의 경우는 트럭기사들이 레미콘을 쏟아 낼 때 슬럼프치가 부족하면 혼화제 대신 물을 추가로 넣어 비빈 후 타설을 하여 강도저하의 치명적 원인을 제공하는 경우가 발생되기도 한다. 이는 설계시 결정되어 모든 구조물 설계에 사용된 설계강도에 크게 미달하는 콘크리트 사용하게 됨으로써 추후 심각한 문제를 야기할 수 있다. 따라서 본 연구에서는 레미콘에 추가로 물을 첨가하는 문제를 해결하고자, 콘크리트에 현장에서 많이 사용되고 있는 혼화제를 정해진 시간간격으로 추가하는 것이 굳은 혹은 굳지 않은 콘크리트의 역학적 특성에 미치는 영향을 검토하여 콘크리트의 슬럼프 유지를 위한 적정한 혼화제 사용에 대한 근거자료를 제시하고자 한다.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2001년도 추계학술발표대회
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• pp.333-335
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• 2001

A simple control strategy of DO-stat was introduced to the recombinant rGuamerin production process in Pichia pastoris. This induction strategy consisted of two interrelated control loops ‘by which oxygen ratio of inlet gas and methanol feeding rate was controlled. Using this control strategy, over-feeding or under-feeding of methanol could be avoided in concomitance with the efficient control of dissolved oxygen level. As a result, the cell concentration reached 130 g/L and rGuamerin expression level was 450 iu/L, which was more than 40% increased result comparing with the fed-batch process using manual control of methanol feeding rate.

• 한국염색가공학회지
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• 제11권6호
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• pp.36-42
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• 1999

The antimicrobial activities of the copolymer of N,N'-dimethyl- N,N'-diallyl ammonium chloride(DMDAAC) and diallyl amino(DA) were investigated. The copolymer of DMDAAC and DA was prepared by free radical Polymerization through an intra-intermolecular propagation mechanism ie, cyclopolymerization. The copolymer was, then, reacted with cyanuric chloride for reactivity with hydroxyl group of cellulose. Cotton fabrics were finished by synthesized polymer, and their antimicrobial activities and fastness to launderings were tested. Dichlorotriazinyl DMDAAC-DA copolymer has MIC value of 1ppm against S. aureus and 10ppm against K pneumoniae. The antimicrobial fastness of the finished cotton to launderings were good enough to show colony reduction above 70% against S. aureus and K pneumoniae after 50 repeated laundering in anionic commercial detergent. Optimum treatment concentrations of the polymer were 0.5% in cold pad-batch method, and 0.1% in pad-dry method.

• 한국미생물·생명공학회지
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• 제24권6호
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• pp.705-711
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• 1996

For the efficient production of 2, 5-diketo-gluconic acid (2, 5-DKG) by the immobilized cells of Erwinia herbicola, basic characteristics of 2, 5-DKG fermentation were analyzed and a process employing immobilized cell reactor was developed. The immobilized cells appeared to have diffusion limitation, and a maximum production of 2, 5-DKG was accomplished with 2 mm diameters of immobilized beads. Long-term stabilities of the immobilized cells could be maintained by addition of 1.75% (w/v) polypep- tone. Repeated batch fermentations with about 80 mol% of 2, 5-DKG yields were carried out six times in the fluidized bubble column reactors filled with immobilized cells at an aeration rate of 6 vvm.

• Fisheries and Aquatic Sciences
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• 제14권3호
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• pp.230-233
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• 2011

To determine the cost-effectiveness of converting fish waste into liquid fertilizer, this study analyzed the production of 3 L of liquid fertilizer from the fermentation of fish waste. The total product cost of the fertilizer was calculated to be $165.26 for a one-batch operation. If the seed culture was repeated five times, the total product cost could be reduced to $36.39/L. According to this analysis, the reutilization of fish waste as liquid fertilizer was not particularly economically attractive at present, and plant-scale production would be necessary for commercialization. This is the first cost-effectiveness analysis of the bioconversion of fish waste into liquid fertilizer.

• 한국식품영양학회지
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• 제2권2호
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• pp.14-20
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• 1989

A strain of Saccharomyces cerevisiae BY-366 was isolated to produce a strong sucrose-hydrolyzing enzyme. After entrapment of yeast cell invertase with alginate, enzymatic properties of immobilized cells were investigated. The results are as follows. 1. The optimum pH of invertase in immobilized cells and non- immobilized cells was 6.0 and 5.0, and pH stability of invertase in immobilized cells and non- immobilized cells was 6.0 and 5.0, respectively. 2 Activation energy of immobilized cells was 4.7 kcal/mol. 3 The immobilized preparation exhibited high resistance to heat and urea Induced denaturation. 4, The bead size less than 2 mm in diameter was desirable. 5. In spite of repeated use, the enzyme activity of immobilized cells was inhibited slightly in batch reaction, and a small column of the immobilized preparation could hydrolyze relatively high concentration of sucrose almost quantitatively to more than 6 days.

• Biotechnology and Bioprocess Engineering:BBE
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• 제8권1호
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• pp.23-31
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• 2003

A mathematical model was formulated to simulate the long-term performance of an anaerobic bioreactor designed to digest Korean food wastes. The system variables of various decomposition steps were built into the model, which predicts the temporal characters of Solid waste, and volatile fatty acid (VFA) in the reactor, and gas production in response to various input loadings and temperatures. The predicted values of VFA and gas production were found to be in good agreement with experimental observations in batch and repeated-input systems. Finally, long-term reactor performance was simulated with respect to the seasonal temperature changes from 5C in winter to 25C in Summer at different food waste input loadings. The simulation results provided us with information concerning the success or failure of a process during long-term operation .

• Korean Chemical Engineering Research
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• 제46권4호
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• pp.665-668
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• 2008

티타늄 함유 제올라이트 촉매 Ti-MCM-22를 이용하여 과산화수소를 산화제로 한 프로필렌 에폭시화반응을 수행하였으며, 반응 온도와 압력, 촉매량, 한계 반응물인 과산화수소의 농도, 산화프로필렌의 첨가, 용매 및 촉매 재생 효과를 조사하였다. Acetonitrile 용매 하에서 반응 실험을 수행한 결과, 99% 이상의 $H_2O_2$ 전화율과 100%에 근접하는 산화프로필렌 선택도를 보였다.

• Journal of Biosystems Engineering
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• 제30권5호
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• pp.312-317
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• 2005

A flow injection type biosensor was tested to confirm the performance of a batch and a continuous type flow injection unit. Reproducibility and consistence of the biosensor were investigated to determine the effect of pulsations and air bubbles, and the applicability of on-line monitoring. The air bubbles affected the performance of the sensor irrespective of the location, and also the pulsations of the pump influenced the performance of the sensor. The applicability of on-line motoring was accepted as the result of the repeated and long-term measurements.