• BMB Reports
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• v.55 no.4
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• pp.175-180
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• 2022

Peptides are gaining substantial attention as therapeutics for human diseases. However, they have limitations such as low bioavailability and poor pharmacokinetics. Periostin, a matricellular protein, can stimulate the repair of ischemic tissues by promoting angiogenesis. We have previously reported that a novel angiogenic peptide (amino acids 142-151) is responsible for the pro-angiogenic activity of periostin. To improve the in vivo delivery efficiency of periostin peptide (PP), we used proteins self-assembled into a hollow cage-like structure as a drug delivery nanoplatform in the present study. The periostin peptide was genetically inserted into lumazine synthase (isolated from Aquifex aeolicus) consisting of 60 identical subunits with an icosahedral capsid architecture. The periostin peptide-bearing lumazine synthase protein cage nanoparticle with 60 periostin peptides multivalently displayed was expressed in Escherichia coli and purified to homogeneity. Next, we examined angiogenic activities of this periostin peptide-bearing lumazine synthase protein cage nanoparticle. AaLS-periostin peptide (AaLS-PP), but not AaLS, promoted migration, proliferation, and tube formation of human endothelial colony-forming cells in vitro. Intramuscular injection of PP and AaLS-PP increased blood perfusion and attenuated severe limb loss in the ischemic hindlimb. However, AaLS did not increase blood perfusion or alleviate tissue necrosis. Moreover, in vivo administration of AaLS-PP, but not AaLS, stimulated angiogenesis in the ischemic hindlimb. These results suggest that AaLS is a highly useful nanoplatform for delivering pro-angiogenic peptides such as PP.

• International Journal of Computer Science & Network Security
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• v.21 no.12
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• pp.309-315
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• 2021

The purpose of the article is to analyze the functioning of economic systems in the context of the development of their potential in a circular economy. It is determined that the functioning of economic systems to ensure their sustainability should meet modern challenges and provide for the formation of competitive institutional architecture, the introduction of structural and regulatory innovations, the transition to an innovative model of development. The specific principles of functioning of economic systems include openness, nonlinearity, multivectority, dynamism, emergence, uncertainty about the development of economic processes. It is substantiated that the linear nature of development and equilibrium are not dominant in the functioning of economic systems, and increasing the level of economic efficiency should go hand in hand with minimizing the activities of enterprises, which necessitates the use of circular economy. The main prerequisites for the transition to a circular economy are analyzed. It is determined that the basic concept of the circular economy involves the development of a system of production and consumption, which is based on processing, reuse, repair, product sharing, change of consumption patterns and new business models and systems. The main elements of the circular economy include: a closed cycle, the use of renewable energy sources, systems thinking. The correlation of the principles of sustainable development and the peculiarities of the application of the circular economy is analyzed. It is determined that the circular economy contrasts with the traditional linear economic model, which is based on the model of "take-do-consume-throw away". The advantages and disadvantages due to the use of the principles of circular economy are given. Based on the study, steps are identified to accelerate the transition from a linear economy to a circular economy.

• Steel and Composite Structures
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• v.44 no.2
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• pp.271-281
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• 2022

This study discusses challenges of running expandable profile liners (EPLs) to isolate trouble zones in directional section of a deep well, and summary the expandable profile liner technology (EPLT) field trial experience. Technically, the trial result reveals that it is feasible to apply the EPLT solving lost-circulation control problem and wellbore instability in the deep directional section. Propose schemes for optimizing the EPLT operation procedure to break through the existing bottleneck of EPLT in the deep directional section. Better-performing transition joints are developed to improve EPL string reliability in high borehole curvature section. High-performing and reliable expanders reduce the number of trips, offer excellent mechanical shaping efficiency, simplify the EPLT operation procedure. Application of the expansion and repair integrated tool could minimize the risk of insufficient expansion and increase the operational length of the EPL string. The new welding process and integrated automatic welding equipment improve the welding quality and EPL string structural integrity. These optimization schemes and recent new advancements in EPLT can bring significant economic benefits and promote the application of EPLT to meet future challenges.

• Journal of the Korean Society of Industry Convergence
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• v.26 no.5
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• pp.855-866
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• 2023

With the rapid advancement and sophistication of defense weapon systems, the government, military, and the defense industry have conducted various innovative attempts to improve the efficiency of post-logistics support(PLS). The Ministry of Defense has mandated RAM-C(Reliability, Availability, and Maintainability-Cost) analysis as a requirement according to revised Total Life Cycle System Management Code of Practice in May 2022. Especially, for the project budget forecast of new PBL(Performance Based Logistics) business contacts, RAM-C is recognized as an obligatory factor. However, relevant entities have not officially provided guidelines or manuals for RAM-C analysis, and each defense contractor conducts RAM-C analysis with different standards and methods to win PBL-related business contract. Hence, this study aims to contribute to the generalization of the analysis procedure by presenting a cost analysis case based on RAM-C for the supply of military depot maintenance PBL project. This study presents formulas and procedures to determine requirements of military depot maintenance PBL project for repair parts supply. Moreover, a sensitivity analysis was conducted to find the optimal cost/utilization ratio. During the process, a correlation was found between supply delay and total cost of ownership as well as between cost variability and utilization rate. The analysis results are expected to provide an important basis for the conceptualization of the cost analysis for the supply of military depot maintenance PBL project and are capable of proposing the optimal utilization rate in relation to cost.

• Asian-Australasian Journal of Animal Sciences
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• v.30 no.8
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• pp.1175-1182
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• 2017

Objective: To create genetically modified goat as a biopharming source of recombinant human lacotoferrin (hLF) with transcription activator-like effector nucleases. Methods: TALENs and targeting vector were transferred into cultured fibroblasts to insert hLF cDNA in the goat beta-lactoglobulin (BLG) locus with homology-directed repair. The gene targeted efficiency was checked using sequencing and TE7I assay. The bi-allelic gene targeted colonies were isolated and confirmed with polymerase chain reaction, and used as donor cells for somatic cell nuclear transfer (SCNT). Results: The targeted efficiency for BLG gene was approximately 10%. Among 12 Bi-allelic gene targeted colonies, five were used in first round SCNT and 4 recipients (23%) were confirmed pregnant at 30 d. In second round SCNT, 7 (53%), 4 (31%), and 3 (23%) recipients were confirmed to be pregnant by ultrasound on 30 d, 60 d, and 90 d. Conclusion: This finding signifies the combined use of TALENs and SCNT can generate biallelic knock-in fibroblasts that can be cloned in a fetus. Therefore, it might lay the foundation for transgenic hLF goat generation and possible use of their mammary gland as a bioreactor for large-scale production of recombinant hLF.

• International Journal of Highway Engineering
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• v.17 no.3
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• pp.85-95
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• 2015

PURPOSES : Recently, bonded concrete overlay has been used as an alternative solution in concrete pavement rehabilitation since its material properties are similar to those of the existing concrete pavements. Deteriorated concrete pavements need rapid rehabilitation in order to prevent traffic jams on Korean expressways. Moreover, speedy and effective repair methods are required. Therefore, the use of bonded concrete overlay with ultra-rapid hardening cement has increased in an effort to reopen promptly the expressways in Korea. However, mobile mixer is required for ultra-rapid hardening cement concrete mixing in the construction site. The use of mobile mixer causes various disadvantages aforementioned such as limitation of the construction supply, open-air storage of mixing materials, increase in construction cost, and etc. In this study, therefore, hydration accelerator in-situ mixing on polymer modified concrete produced in concrete plant is attempted in order to avoid the disadvantages of existing bonded concrete overlay method using ultra-rapid hardening cement. METHODS : Bonded concrete overlay materials using ultra-rapid hardening cement should be meet all the requirements including structural characteristics, compatibility, durability for field application. Therefore, This study aimed to evaluate the application of hydration accelerator in-situ mixing on polymer modified concrete by evaluating structural characteristics, compatibility, durability and economic efficiency for bonded concrete overlay. RESULTS : Test results of structural characteristics showed that the compressive, flexural strength and bond strength were exceed 21MPa, 3.15MPa and 1.4MPa, respectively, which are the target strengths of four hours age for the purpose of prompt traffic reopening. In addition, tests of compatibility, such as drying shrinkage, coefficient of thermal expansion and modulus of elasticity, and durability (chloride ions penetration resistance, freezing-thawing resistance, scaling resistance, abrasion resistance and crack resistance), showed that the hydration accelerator in-situ mixing on polymer modified concrete were satisfied the required criteria. CONCLUSIONS : It was known that the hydration accelerator in-situ mixing on polymer modified concrete overlay method was applicable for bonded concrete overlay and was a good alternative method to substitute the existing bonded concrete overlay method since structural characteristics, compatibility, durability were satisfied the criteria and its economic efficiency was excellent compare to the existing bonded concrete overlay methods.

• Journal of the Korea Society of Computer and Information
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• v.14 no.8
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• pp.145-152
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• 2009

Manufacturer's competitiveness in the MRO industry, which is to stimulate the growth of the business-to-business e-commerce market, has recently become more important. A nuclear parts manufacturer was supplying products based on irregular demand from clients which differs from ordinary MRO business practices. The reason for this is the Nuclear Parts Manufacturer has fallen behind the e-commerce performance of other industries, and they lack global competitiveness due to the low efficiency of the individual companies within it. In this study, we developed an MRO based a-Marketplace system to minimize repetitive ordering of raw materials, lack of reusability and inefficiency of transaction processing which was a result of the former legacy business practice, In order to accomplish the purpose of this study, we implemented a web based automated CR system which considered the characteristics of the nuclear parts manufacturing: the system has sub modules such as ordering, product management, transaction management, warehousing and raw material handling. As a consequence of the system implementation, H corporate successfully automated ordering of raw materials, quotation processing and inventory management compared to the legacy business process, achieving increased efficiency by reducing wasteful resources.

• Proceedings of the Korean Institute of Navigation and Port Research Conference
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• 2020.11a
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• pp.163-164
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• 2020

Along with the increase in global volume of goods, logistics companies are trying to reduce costs by increasing the size of ships carrying cargo, increasing the efficiency of quantitative equipment at ports, and unmanned electric vehicles on land. Korean naval ports are also facing the same situation as the global trend. In the past, small and medium-sized ships such as FF, PCC, and beheaded eagles are being retired for their longevity, and their positions are being replaced by large ships such as KDX, FFG, and LST-II. In particular, large ships such as Dokdo and the next light aircraft carriers are also being prepared. Unlike general merchant ships, naval ships require periodic inspections and preventive maintenance, so repair piers such as maintenance depots are in operation. The naval maintenance depot mainly uses trailers, trucks, and truck-type cranes to carry out loading and unloading of heavy ships, and the application or development of automation and unmanned equipment performed by the private sector is inadequate, and self-improvement cases are also very weak. This study aims to improve the efficiency of the military logistics system through research on the logistics system such as transport, storage and unloading of heavy goods and maintenance products of naval ships.

• Animal Bioscience
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• v.37 no.5
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• pp.929-943
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• 2024

Objective: The present study was executed to explore the molecular mechanism of fibroblast growth factor 10 (FGF10) gene in bovine adipogenesis. Methods: The bovine FGF10 gene was overexpressed through Ad-FGF10 or inhibited through siFGF10 and their negative control (NC) in bovine adipocytes, and the multiplicity of infection, transfection efficiency, interference efficiency were evaluated through quantitative real-time polymerase chain reaction, western blotting and fluorescence microscopy. The lipid droplets, triglycerides (TG) content and the expression levels of adipogenic marker genes were measured during preadipocytes differentiation. The differentially expressed genes were explored through deep RNA sequencing. Results: The highest mRNA level was found in omasum, subcutaneous fat, and intramuscular fat. Moreover, the highest mRNA level was found in adipocytes at day 4 of differentiation. The results of red-oil o staining showed that overexpression (Ad-FGF10) of the FGF10 gene significantly (p<0.05) reduced the lipid droplets and TG content, and their down-regulation (siFGF10) increased the measurement of lipid droplets and TG in differentiated bovine adipocytes. Furthermore, the overexpression of the FGF10 gene down regulated the mRNA levels of adipogenic marker genes such as CCAAT enhancer binding protein alpha (C/EBPα), fatty acid binding protein (FABP4), peroxisome proliferator-activated receptor-γ (PPARγ), lipoprotein lipase (LPL), and Fas cell surface death receptor (FAS), similarly, down-regulation of the FGF10 gene enriched the mRNA levels of C/EBPα, PPARγ, FABP4, and LPL genes (p<0.01). Additionally, the protein levels of PPARγ and FABP4 were reduced (p<0.05) in adipocytes infected with Ad-FGF10 gene and enriched in adipocytes transfected with siFGF10. Moreover, a total of 1,774 differentially expressed genes (DEGs) including 157 up regulated and 1,617 down regulated genes were explored in adipocytes infected with Ad-FGF10 or Ad-NC through deep RNA-sequencing. The top Kyoto encyclopedia of genes and genomes pathways regulated through DEGs were the PPAR signaling pathway, cell cycle, base excision repair, DNA replication, apoptosis, and regulation of lipolysis in adipocytes. Conclusion: Therefore, we can conclude that the FGF10 gene is a negative regulator of bovine adipogenesis and could be used as a candidate gene in marker-assisted selection.

• Membrane Journal
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• v.21 no.1
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• pp.22-29
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• 2011

During the maintenance, repair and replacement process of circuit breaker, $SF_6$ reacted with input air in arc discharge, which led to the production of by-product gases (eg, $N_2$, $O_2$, $CF_4$, $SO_2$, $H_2O$, HF, $SOF_2$, $CuF_2$, $WO_3$). Among these various by-product gases, $N_2$, $O_2$, $CF_4$ is major component. Therefore, the effective separation process is necessary to recycle the $SF_6$ gas from the mixture gas containing $N_2$, $O_2$, $CF_4$. In this study, the membrane separation process was applied to recycle the $SF_6$ gas from the mixture gas containing $N_2$, $O_2$, $CF_4$. The concentration of $SF_6$ gas in gas produced from the electric power industry is over than 90 vol%. Therefore, we made the simulated gas containing $N_2$, $O_2$, $CF_4$, $SF_6$ which the concentration of $SF_6$ gas is minimum 90 vol%. From the results of membrane separation process of $SF_6$ gas from $N_2$, $O_2$, $CF_4$ $SF_6$ mixture gases, PSF membrane shown the highest recovery efficiency 92.7%, in $25^{\circ}C$ and 150 cc/min of retentate flow rate. On the other hand, PC membrane shown the highest recovery efficiency 74.8%, in $45^{\circ}C$ and 150 cc/min of retentate flow rate. Also, the highest rejection rate of $N_2$, $O_2$, $CF_4$ is 80, 74 and 58.9% seperately in the same operation condition of highest recovery efficiency. From the results, we supposed the membrane separation process as the effective $SF_6$ separation and recycle process from the mixture gas containing $N_2$, $O_2$, $CF_4$, $SF_6$.