• BMB Reports
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• 제55권4호
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• pp.175-180
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• 2022

Peptides are gaining substantial attention as therapeutics for human diseases. However, they have limitations such as low bioavailability and poor pharmacokinetics. Periostin, a matricellular protein, can stimulate the repair of ischemic tissues by promoting angiogenesis. We have previously reported that a novel angiogenic peptide (amino acids 142-151) is responsible for the pro-angiogenic activity of periostin. To improve the in vivo delivery efficiency of periostin peptide (PP), we used proteins self-assembled into a hollow cage-like structure as a drug delivery nanoplatform in the present study. The periostin peptide was genetically inserted into lumazine synthase (isolated from Aquifex aeolicus) consisting of 60 identical subunits with an icosahedral capsid architecture. The periostin peptide-bearing lumazine synthase protein cage nanoparticle with 60 periostin peptides multivalently displayed was expressed in Escherichia coli and purified to homogeneity. Next, we examined angiogenic activities of this periostin peptide-bearing lumazine synthase protein cage nanoparticle. AaLS-periostin peptide (AaLS-PP), but not AaLS, promoted migration, proliferation, and tube formation of human endothelial colony-forming cells in vitro. Intramuscular injection of PP and AaLS-PP increased blood perfusion and attenuated severe limb loss in the ischemic hindlimb. However, AaLS did not increase blood perfusion or alleviate tissue necrosis. Moreover, in vivo administration of AaLS-PP, but not AaLS, stimulated angiogenesis in the ischemic hindlimb. These results suggest that AaLS is a highly useful nanoplatform for delivering pro-angiogenic peptides such as PP.

• International Journal of Computer Science & Network Security
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• 제21권12호
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• pp.309-315
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• 2021

The purpose of the article is to analyze the functioning of economic systems in the context of the development of their potential in a circular economy. It is determined that the functioning of economic systems to ensure their sustainability should meet modern challenges and provide for the formation of competitive institutional architecture, the introduction of structural and regulatory innovations, the transition to an innovative model of development. The specific principles of functioning of economic systems include openness, nonlinearity, multivectority, dynamism, emergence, uncertainty about the development of economic processes. It is substantiated that the linear nature of development and equilibrium are not dominant in the functioning of economic systems, and increasing the level of economic efficiency should go hand in hand with minimizing the activities of enterprises, which necessitates the use of circular economy. The main prerequisites for the transition to a circular economy are analyzed. It is determined that the basic concept of the circular economy involves the development of a system of production and consumption, which is based on processing, reuse, repair, product sharing, change of consumption patterns and new business models and systems. The main elements of the circular economy include: a closed cycle, the use of renewable energy sources, systems thinking. The correlation of the principles of sustainable development and the peculiarities of the application of the circular economy is analyzed. It is determined that the circular economy contrasts with the traditional linear economic model, which is based on the model of "take-do-consume-throw away". The advantages and disadvantages due to the use of the principles of circular economy are given. Based on the study, steps are identified to accelerate the transition from a linear economy to a circular economy.

• Steel and Composite Structures
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• 제44권2호
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• pp.271-281
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• 2022

This study discusses challenges of running expandable profile liners (EPLs) to isolate trouble zones in directional section of a deep well, and summary the expandable profile liner technology (EPLT) field trial experience. Technically, the trial result reveals that it is feasible to apply the EPLT solving lost-circulation control problem and wellbore instability in the deep directional section. Propose schemes for optimizing the EPLT operation procedure to break through the existing bottleneck of EPLT in the deep directional section. Better-performing transition joints are developed to improve EPL string reliability in high borehole curvature section. High-performing and reliable expanders reduce the number of trips, offer excellent mechanical shaping efficiency, simplify the EPLT operation procedure. Application of the expansion and repair integrated tool could minimize the risk of insufficient expansion and increase the operational length of the EPL string. The new welding process and integrated automatic welding equipment improve the welding quality and EPL string structural integrity. These optimization schemes and recent new advancements in EPLT can bring significant economic benefits and promote the application of EPLT to meet future challenges.

• 한국산업융합학회 논문집
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• 제26권5호
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• pp.855-866
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• 2023

With the rapid advancement and sophistication of defense weapon systems, the government, military, and the defense industry have conducted various innovative attempts to improve the efficiency of post-logistics support(PLS). The Ministry of Defense has mandated RAM-C(Reliability, Availability, and Maintainability-Cost) analysis as a requirement according to revised Total Life Cycle System Management Code of Practice in May 2022. Especially, for the project budget forecast of new PBL(Performance Based Logistics) business contacts, RAM-C is recognized as an obligatory factor. However, relevant entities have not officially provided guidelines or manuals for RAM-C analysis, and each defense contractor conducts RAM-C analysis with different standards and methods to win PBL-related business contract. Hence, this study aims to contribute to the generalization of the analysis procedure by presenting a cost analysis case based on RAM-C for the supply of military depot maintenance PBL project. This study presents formulas and procedures to determine requirements of military depot maintenance PBL project for repair parts supply. Moreover, a sensitivity analysis was conducted to find the optimal cost/utilization ratio. During the process, a correlation was found between supply delay and total cost of ownership as well as between cost variability and utilization rate. The analysis results are expected to provide an important basis for the conceptualization of the cost analysis for the supply of military depot maintenance PBL project and are capable of proposing the optimal utilization rate in relation to cost.

• Asian-Australasian Journal of Animal Sciences
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• 제30권8호
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• pp.1175-1182
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• 2017

Objective: To create genetically modified goat as a biopharming source of recombinant human lacotoferrin (hLF) with transcription activator-like effector nucleases. Methods: TALENs and targeting vector were transferred into cultured fibroblasts to insert hLF cDNA in the goat beta-lactoglobulin (BLG) locus with homology-directed repair. The gene targeted efficiency was checked using sequencing and TE7I assay. The bi-allelic gene targeted colonies were isolated and confirmed with polymerase chain reaction, and used as donor cells for somatic cell nuclear transfer (SCNT). Results: The targeted efficiency for BLG gene was approximately 10%. Among 12 Bi-allelic gene targeted colonies, five were used in first round SCNT and 4 recipients (23%) were confirmed pregnant at 30 d. In second round SCNT, 7 (53%), 4 (31%), and 3 (23%) recipients were confirmed to be pregnant by ultrasound on 30 d, 60 d, and 90 d. Conclusion: This finding signifies the combined use of TALENs and SCNT can generate biallelic knock-in fibroblasts that can be cloned in a fetus. Therefore, it might lay the foundation for transgenic hLF goat generation and possible use of their mammary gland as a bioreactor for large-scale production of recombinant hLF.

• 한국도로학회논문집
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• 제17권3호
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• pp.85-95
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• 2015

PURPOSES : Recently, bonded concrete overlay has been used as an alternative solution in concrete pavement rehabilitation since its material properties are similar to those of the existing concrete pavements. Deteriorated concrete pavements need rapid rehabilitation in order to prevent traffic jams on Korean expressways. Moreover, speedy and effective repair methods are required. Therefore, the use of bonded concrete overlay with ultra-rapid hardening cement has increased in an effort to reopen promptly the expressways in Korea. However, mobile mixer is required for ultra-rapid hardening cement concrete mixing in the construction site. The use of mobile mixer causes various disadvantages aforementioned such as limitation of the construction supply, open-air storage of mixing materials, increase in construction cost, and etc. In this study, therefore, hydration accelerator in-situ mixing on polymer modified concrete produced in concrete plant is attempted in order to avoid the disadvantages of existing bonded concrete overlay method using ultra-rapid hardening cement. METHODS : Bonded concrete overlay materials using ultra-rapid hardening cement should be meet all the requirements including structural characteristics, compatibility, durability for field application. Therefore, This study aimed to evaluate the application of hydration accelerator in-situ mixing on polymer modified concrete by evaluating structural characteristics, compatibility, durability and economic efficiency for bonded concrete overlay. RESULTS : Test results of structural characteristics showed that the compressive, flexural strength and bond strength were exceed 21MPa, 3.15MPa and 1.4MPa, respectively, which are the target strengths of four hours age for the purpose of prompt traffic reopening. In addition, tests of compatibility, such as drying shrinkage, coefficient of thermal expansion and modulus of elasticity, and durability (chloride ions penetration resistance, freezing-thawing resistance, scaling resistance, abrasion resistance and crack resistance), showed that the hydration accelerator in-situ mixing on polymer modified concrete were satisfied the required criteria. CONCLUSIONS : It was known that the hydration accelerator in-situ mixing on polymer modified concrete overlay method was applicable for bonded concrete overlay and was a good alternative method to substitute the existing bonded concrete overlay method since structural characteristics, compatibility, durability were satisfied the criteria and its economic efficiency was excellent compare to the existing bonded concrete overlay methods.

• 한국컴퓨터정보학회논문지
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• 제14권8호
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• pp.145-152
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• 2009

기업간 전자상거래의 성장을 견인하는 MRO산업에서의 제조업 경쟁력이 점차 중요시되고 있다. 일반적인 형태의 MRO와는 달리 원전부품제조업은 수요와 공급측면에서 철저히 부정기적인 형태를 보이고 있다. 이와 같은 구조적인 특징으로 인해 완전부품제조업은 타 산업에 비해 상대적으로 전자상거래 추진이 미흡하며 개별기업의 효율성이 낮아 국제적인 경쟁력을 갖추지 못하고 있다. 본 연구에서는 원전부품제조업 MRO를 수행하는 H기업에 존재하던 원부자재 중복발주, 잔여재고 재사용성 부재, 재고관리 및 거래관리의 비효율성 등의 문제를 최소화하기 위해 CR 기반의 e-Marketplace 시스템을 구현하였다. 성공적인 시스템 구현을 위해 첫째, 원전부품제조업의 특성을 반영하고 들째, H기업의 철저한 사용자 요구에 따라 수 발주관리, 상품관리, 거래관리, 입출고관리, 원자재관리 등 각 하위모듈을 통합한 웹 기반 시스템을 개발하여 CR을 구현하고자 하였다. 결과적으로 본 연구를 통해 H기업은 기존의 업무처리방식에 비해 시간과 비용측면의 효율성을 현저히 개선하고, 원부자재 매입매출과 관련한 CR을 성공적으로 자동화하였으며, 잔여재고 재사용 등 자원요소의 낭비요소를 제거하였다.

• 한국항해항만학회:학술대회논문집
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• 한국항해항만학회 2020년도 추계학술대회
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• pp.163-164
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• 2020

세계적인 물동량의 증가와 함께 물류기업들은 원가 절감을 위해 화물을 운송하는 선박의 대형화와 항만의 양적화 장비의 고효율화 그리고 육상에서의 운송장비 역시 무인화 전동화가 되는 추세이다. 우리나라 해군 항만 역시 세계적인 추세와 같은 상황에 직면해있다. 과거 FF나 PCC, 참수리 등의 중소형 함정은 내구연한으로 퇴역하고 있으며 그 자리를 KDX, FFG, LST-II 등 대형 함정으로 대체되고 있으며 특히 독도함과 차기 경항공모함과 같은 대형 함정 역시 준비되고 있다. 해군 함정은 일반 상선과는 달리 주기적인 점검과 예방 정비가 필요하여 정비창과 같은 수리부두가 운영되고 있다. 해군 정비창에서는 주로 트레일러, 트럭, 트럭형 크레인을 주로 사용하여 함정 중량물 양적하 작업을 진행 중에 있으며 민간에서 행하는 자동화 및 무인화 장비의 적용이나 개발은 미비하며 자체 개선 사례 역시 매우 미약한 실정이다. 본 연구에서는 해군함정의 중량품 정비품 이송, 보관 및 하역 등의 물류체계에 관한 연구를 통하여, 군 물류체계의 효율화를 행하고자 한다.

• Animal Bioscience
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• 제37권5호
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• pp.929-943
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• 2024

Objective: The present study was executed to explore the molecular mechanism of fibroblast growth factor 10 (FGF10) gene in bovine adipogenesis. Methods: The bovine FGF10 gene was overexpressed through Ad-FGF10 or inhibited through siFGF10 and their negative control (NC) in bovine adipocytes, and the multiplicity of infection, transfection efficiency, interference efficiency were evaluated through quantitative real-time polymerase chain reaction, western blotting and fluorescence microscopy. The lipid droplets, triglycerides (TG) content and the expression levels of adipogenic marker genes were measured during preadipocytes differentiation. The differentially expressed genes were explored through deep RNA sequencing. Results: The highest mRNA level was found in omasum, subcutaneous fat, and intramuscular fat. Moreover, the highest mRNA level was found in adipocytes at day 4 of differentiation. The results of red-oil o staining showed that overexpression (Ad-FGF10) of the FGF10 gene significantly (p<0.05) reduced the lipid droplets and TG content, and their down-regulation (siFGF10) increased the measurement of lipid droplets and TG in differentiated bovine adipocytes. Furthermore, the overexpression of the FGF10 gene down regulated the mRNA levels of adipogenic marker genes such as CCAAT enhancer binding protein alpha (C/EBPα), fatty acid binding protein (FABP4), peroxisome proliferator-activated receptor-γ (PPARγ), lipoprotein lipase (LPL), and Fas cell surface death receptor (FAS), similarly, down-regulation of the FGF10 gene enriched the mRNA levels of C/EBPα, PPARγ, FABP4, and LPL genes (p<0.01). Additionally, the protein levels of PPARγ and FABP4 were reduced (p<0.05) in adipocytes infected with Ad-FGF10 gene and enriched in adipocytes transfected with siFGF10. Moreover, a total of 1,774 differentially expressed genes (DEGs) including 157 up regulated and 1,617 down regulated genes were explored in adipocytes infected with Ad-FGF10 or Ad-NC through deep RNA-sequencing. The top Kyoto encyclopedia of genes and genomes pathways regulated through DEGs were the PPAR signaling pathway, cell cycle, base excision repair, DNA replication, apoptosis, and regulation of lipolysis in adipocytes. Conclusion: Therefore, we can conclude that the FGF10 gene is a negative regulator of bovine adipogenesis and could be used as a candidate gene in marker-assisted selection.

• 멤브레인
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• 제21권1호
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• pp.22-29
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• 2011

중전기의 유지 보수 및 교체 과정에서 절연체로 사용된 $SF_6$ 가스는 교체 충전과 정제과정에서 유입되는 공기와의 아크 방전에 의해 많은 종류의 부산물($N_2$, $O_2$, $CF_4$, $SO_2$, $H_2O$, HF, $SOF_2$, $CuF_2$, $WO_3$ 등)이 발생된다. 부산물 중에서도 대부분을 차지하는 것이 $N_2$, $O_2$, $CF_4$이며, $SF_6$가스를 재사용하기 위해서는 이들을 효과적으로 분리 회수하는 공정이 필요하다. 주요 부산물인 $N_2$, $O_2$, $CF_4$와의 분리 효율 측면에서 분리막법은 기존의 흡착, 심냉법에 비하여 상대적으로 높은 효율을 보이고 있어 이에 대한 관심 또한 증가하고 있다. 따라서 본 논문에서는 중전기 산업에서 발생되는 $SF_6$ 가스 함유 농도 90 vol% 이상의 가스에 대하여 분리막법을 적용하여 $N_2$, $O_2$, $CF_4$와 $SF_6$ 가스의 온도와 배출유량의 변화에 따른 분리 회수 가능성을 관찰하였다. PSF와 PC 중공사 분리막을 이용하여 고농도 $SF_6$에 대한 분리 회수 실험 결과, PSF 분리막의 최대 회수율은 압력 0.3 MPa, 온도 $25^{\circ}C$, 배출유량 150 cc/min에서 92.7%를 나타내었으며, PC 분리막에서는 압력 0.3 MPa, 온도 $45^{\circ}C$, 배출유량 150 cc/min 일 때 74.8%의 최대 회수율을 나타내었다. 또한, 사용된 두 가지 분리막과 운전 조건에서 주요 부산물인 $N_2$, $O_2$, $CF_4$의 최대 제거율은 각각 약 80%, 74%, 그리고 58.9%가 관찰되었다. 이로부터 분리막 공정은 고농도 폐 $SF_6$ 가스에서 주요 부산물로부터 $SF_6$의 효과적인 분리 및 회수가 가능한 공정으로 적용할 수 있는 가능성을 파악 할 수 있었다.