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Microbial Inhibition Test of Sustained-Release Chlorine Dioxide Gas Freshness Retaining Agent

  • Choe, Yoowha
    • International Journal of Advanced Culture Technology
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    • v.8 no.3
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    • pp.211-215
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    • 2020
  • Currently, most of the chlorine dioxide gas is processed at the beginning of storage or distribution. It has the disadvantage of not being able to continuously process gas since there is no system that can continuously process it during the distribution process. Therefore, in order to minimize changes in freshness and quality during the distribution process of agrifood, there is a need for a sustained-release chlorine dioxide gas treatment technology that can be continuously released. Therefore, in this study, the film to be used was examined so that the chlorine dioxide gas can be continuously released for a certain period of time, the concentration of the reactant and the viscosity at the time of the reaction were determined, and a chlorine dioxide gas gel pack was manufactured using this optimal condition. In addition, the gel pack was used to measure the amount of chlorine dioxide gas released and the sterilization effect of food poisoning bacteria.

Effect analysis of ISLOCA pathways on fission product release at Westinghouse 2-loop PWR using MELCOR

  • Kim, Seungwoo;Park, Yerim;Jin, Youngho;Kim, Dong Ha;Jae, Moosung
    • Nuclear Engineering and Technology
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    • v.53 no.9
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    • pp.2878-2887
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    • 2021
  • As the amount of fission product released from ISLOCA was overestimated because of conservative assumptions in the past, several studies have been recently conducted to evaluate the actual release amount. Among several pathways for the ISLOCA, most studies were focused on the pathway with the highest possibility. However, different ISLOCA pathways may have different fission product release characteristics. In this study, fission product behavior was analyzed for various pathways at the Westinghouse two-loop plant using MELCOR. Four pathways are considered: the pipes from a cold leg, from a downcomer, from a hot leg to the outlet of RHR heat exchanger, and the pipe from the hot leg to the inlet of RHR pump (Pathway 1-4). According to the analysis results, cladding fails at around 2.5 h in Pathways 1 and 2, and on the other hand, about 3.3 h in Pathways 3 and 4 because the ISLOCA pathways affect the safety injection flow path. While the release amount of cesium and iodine ranges between 20 and 26% in Pathways 1 to 3, Pathway 4 allows only 5% to the environment because the break location is submerged. Also, as more than 90% of cesium released to the environment passes through the personnel door, reinforcing the pressure capacity of the doors would be a significant factor in the accident management of the ISLOCA.

Sterilization Test of Microorganisms of Slow-released ClO2 Gas Gel-Pack (서방출형 이산화염소 가스 젤팩의 미생물 살균 시험)

  • Lee, Kyung-Haeng;Kim, Hong-Gil
    • The Korean Journal of Food And Nutrition
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    • v.31 no.2
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    • pp.308-312
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    • 2018
  • Even though chlorine dioxide ($ClO_2$) is utilized in a pre-treatment due to its effective sterilizing activity for microorganisms and its safety for food, it has a limitation in maintaining freshness of the food product. In this study, a low-concentration $ClO_2$ gas was produced in a packaging form of air-permeable gel pack so that it could be released continuously over several days. The amount of $ClO_2$ gas emission and microbial inactivation effect against foodborne pathogens were measured during the release of $ClO_2$ gas. As a result of measuring the change of color in order to confirm whether the chlorine dioxide gas was eluted in the form of a sustained release, the yellowness was significantly higher at higher gel pack concentration and higher value during storage periods. The slow-released $ClO_2$ gel-pack showed clear inactivation effect against Escherichia coli and Staphylococcus aureus with 99.9% inactivation efficiency. As a result of measuring the sterilization effect of Listeria monocytogenes by the concentration of chlorine dioxide gas, the sterilization effect was increased as the concentration was increased. Therefore, the slow-released $ClO_2$ gel-pack is feasible to apply for industry usages.

Formation of Indole Glucosinolates Breakdown Products during Processing Treatment in Cruciferous Vegetables (십자화과 채소의 가공처리 중 Indole Glucosinolate의 분해산물 형성)

  • Shim, Ki-Hwan;Kang, Kap-Suk;Sung, Nack-Kie;Seo, Kwon-Il;Moon, Ju-Seok
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.21 no.1
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    • pp.49-53
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    • 1992
  • The released amount of thiocyanate ion in Cruciferous vegetables treated by wet heat, increased as the reaction time goes by and was maximum value after treatment for 30min, but it was not changed by dry heat treatment. When samples were autolyzed by myrosinase, the amount of thiocyanate ion increased gradually as time goes by, was maximum value after 3hrs and much higher than those in the sample treated by wet. The released amount of thiocyanate ion in each sample showed much in cabbage, Chinese cabbage, radish, kale and mustard in the order. The generated amount of indoleacetonitrile by heat treatment increased as time goes by and the generated amount in each sample determined was high in the order of cabbage, Chinese cabbage and radish.

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Underground Nuclear Test and Crustal Deformation (핵실험과 지각변동)

  • Kim, Ik-Gon
    • Journal of the Korean Professional Engineers Association
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    • v.44 no.1
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    • pp.44-47
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    • 2011
  • Huge amount of energy produced by an underground nuclear test is released into the surrounding rock. Depending on the properties of the bed rock surrounding the detonation and overlaying it a variety of effects can occur. At some particular depth the increasing amount of material thrown upward is exactly balanced by the decreasing fraction escaping the crater, the crater volume reaches to a maximum. This depth is called the optimum depth of burial and varies somewhat with the geology of the site, being greater for less dense and structurally weaker material.

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Controlled Release of Nerve Growth Factor from Sandwiched Poly(L-lactide-co-glycolide) Films for the Application in Neural Tissue Engineering

  • Gilson Khang;Jeon, Eun-Kyung;John M. Rhee;Lee, Ilwoo;Lee, Sang-Jin;Lee, Hai-Bang
    • Macromolecular Research
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    • v.11 no.5
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    • pp.334-340
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    • 2003
  • In order to fabricate new sustained delivery device of nerve growth factor (NGF), we developed NGF-loaded biodegradable poly(L-lactide-co-glycolide) (PLGA, the mole ratio of lactide to glycolide 75:25, molecular weight: 83,000 and 43,000 g/mole, respectively) film by novel and simple sandwich solvent casting method for the possibility of the application of neural tissue engineering. PLGA was copolymerized by direct condensation reaction and the molecular weight was controlled by reaction time. Released behavior of NGF from NGF-loaded films was characterized by enzyme linked immunosorbent assay (ELISA) and degradation characteristics were observed by scanning electron microscopy (SEM) and gel permeation chromatography (GPC). The bioactivity of released NGF was identified using a rat pheochromocytoma (PC-12) cell based bioassay. The release of NGF from the NGF-loaded PLGA films was prolonged over 35 days with zero-order rate of 0.5-0.8 ng NGF/day without initial burst and could be controlled by the variations of molecular weight and NGF loading amount. After 7 days NGF released in phosphate buffered saline and PC-12 cell cultured on the NGF-loaded PLGA film for 3 days. The released NGF stimulated neurite sprouting in cultured PC-12 cells, that is to say, the remained NGF in the NGF/PLGA film at 37 $^{\circ}C$ for 7 days was still bioactive. This study suggested that NGF-loaded PLGA sandwich film is released the desired period in delivery system and useful neuronal growth culture as nerve contact guidance tube for the application of neural tissue engineering.

Evaluation of biodegradability according to bait type for crab pots (꽃게 통발용 미끼의 형태에 대한 생분해도 평가)

  • Jeong, Byung-Gon;Chang, Ho-Young;Koo, Jae-Geun
    • Journal of the Korean Society of Fisheries and Ocean Technology
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    • v.44 no.1
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    • pp.20-30
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    • 2008
  • In order to evaluate the biodegradability of bait used in the pot for swimming crab, water tank experiments were conducted. Mackerel is the most commonly used natural form of bait to catch the swimming crabs, and therefore was used in this experiment for the biodegradability according to the manufacturing process of the bait. From the biodegradability test on chemical oxygen demand(COD), total nitrogen(T - N), total phosphorus(T - P), ammoniac nitrogen, nitrite nitrogen and nitrate nitrogen per unit weight of the bait based on the production rate and the accumulated amount of pollutants, it was concluded that the smaller the size of the mackerel pieces, the higher the production rate and accumulated amount of organic matter and nutrients which was unfavorable to water pollution. The amount of pollutants released from the intestine of the tuna was similar with that from the whole mackerel. For the operation period of 111 days, the accumulated concentrations of tested pollutants from the tuna which were 67.3 mgCOD/g d, 86.4 mgT N/g d, 3.1 mgT - P/g d, were almost half comparing with those from the mackerel which were 65.7 - 94.4 mgCOD/g d, 83.8 - 109.4 mgT - N/g d, 3.1 - 5.2 mgT - P/g d. The amount of pollutants released from the intestine of the tuna was slightly less than that from the mackerel that was cut into 8 pieces. but more than that from the mackerel which was not cut into pieces. Therefore, it can be concluded that the key factor in determining water pollution potential is not the kind of bait, but the processing or preparation method used.

Hydrogen Embrittlement Resistance and Diffusible Hydrogen Desorption Behavior of Multipass FCA Weld Metals (다층 FCA 용착금속의 수소취성 저항성 및 확산성 수소 방출 거동)

  • Yoo, Jaeseok;Xian, Guo;Lee, Myungjin;Kim, Yongdeok;Kang, Namhyun
    • Journal of Welding and Joining
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    • v.31 no.6
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    • pp.112-118
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    • 2013
  • In this study, constant loading test (CLT) was performed to evaluate the hydrogen embrittlement resistance for multipass FCA weld metals of 600MPa tensile strength grade. The microstructures of weld metal-2 having the smallest carbon equivalent (Ceq=0.37) consisted of grain boundary ferrite and widmanstatten ferrite in the acicular ferrite matrix. The weld metal-1 having the largest Ceq=0.47, showed the microstructures of grain boundary ferrite, widmanstatten ferrite and the large amount of bainite (vol.%=19%) in the acicular ferrite matrix. The weld metal-3 having the Ceq=0.41, which was composed of grain boundary ferrite, widmanstatten ferrite, and the small amount of bainite (vol.%=9%) in the acicular ferrite matrix. Hydrogen desorption spectrometry (TDS) used to analyze the amount of diffusible hydrogen and trapping site for the hydrogen pre-charged specimens electrochemically for 24 hours. With increasing the current density of hydrogen pre-charging, the released amount of diffusible hydrogen was increased. Furthermore, as increasing carbon equivalent of weld metals, the released diffusible hydrogen was increased. The main trapping sites of diffusible hydrogen for the weld metal having a low carbon equivalent (Ceq=0.37) were grain boundaries and those of weld metals having a relatively high carbon equivalent (Ceq: 0.41~0.47) were grain boundaries and dislocation. The fracture time for the hydrogen pre-charged specimens in the constant loading test was decreased as the carbon equivalent increased from 0.37 to 0.47. This result is mainly due to the increment of bainite that is vulnerable to hydrogen embrittlement.

Biological Phosphorus and Nitrogen Removal in Anaerobic-Aerobic Activated Sludge Process (활성오니를 이용한 인 및 질소의 생물학적 제거)

  • CHOI Seung-Tae;PARK Mi-Yeon;CHANG Dong-Suck
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.27 no.6
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    • pp.690-695
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    • 1994
  • Simultaneous removal of phosphorus and nitrogen from wastewater was studied by the anaerobic-aerobic system of activated sludge. In the anaerobic stage, most of the influent glucose was removed and orthophosphate was released, when the nitrate and/or nitrite concentration in the wastewater was almost zero. The amount of the released phosphorus was found to be directly proportional to the amount of the removed glucose. When the ratio of phosphorus to glucose in the influent was less than 0.04, the phosphorus in the wastewater was almost completely removed during the aerobic state. Under the anaerobic condition, activated sludge released phosphate and excess removal of phosphate occurred during the aerobic condition. Namely, the stress received in anaerobic period stimulated the uptake of phosphorus in aerobic period. The amounts of phosphorus release in the anaerobic and uptake in the aerobic stage were less in proportional to the concentration of $NO_x-N$. Further, if the initial ratio of $NO_2-N$/glucose was less than 0.37, the inorganic nitrogen in the influent could be completely removed.

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Antigenicity of Protein Entrapped in Poly(lactide-co-glycolide) Microspheres (폴리락티드-글리콜리드 마이크로스피어에 봉입된 단백질의 항원성 평가)

  • Song, Seh-Hyon;Cho, Seong-Wan;Shin, Taek-Hwan;Yoon, Mi-Kyoung;Choi, Young-Wook
    • Journal of Pharmaceutical Investigation
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    • v.31 no.3
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    • pp.191-196
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    • 2001
  • Biodegradable polymeric microspheres were studied for their usefulness as carriers for the delivery of vaccine antigens. However, protein antigen could be denatured during microencapsulation processes due to the exposure to the organic phase and stress condition of cavitation and shear force. Therefore this study was carried out to re-evaluate the degree of protein denaturation during microencapsulation with poly(lactide-co-glycolide) (PLGA) copolymer. PLGA microspheres containing ovalbumin (OVA), prepared by W/O/W multiple emulsification method, were suspended in pH 7.4 PBS and incubated with shaking at $37.5^{\circ}C$. Drug released medium was collected periodically and analyzed for protein contents by micro-BCA protein assay. In order to evaluate the protein integrity, release medium was subjected to the analyses of SDS-PAGE and size exclusion chromatography (SEC). And enzyme-linked immunosorbent assay (ELISA) was introduced to measure the immunoreactivity of entrapped OVA and to get an insight into the three-dimensional structure of epitope. The structures of entrapped protein were not affected significantly by the results of SDS-PAGE and SEC. However, immunoreactivity of released antigen was varied, revealing the possibility of protein denaturation in some microspheres when it was evaluate by ELISA method. Therefore, in order to express the degree of protein denaturation, antigenicity ratio (AR) was obtained as follows: amount of immunoreactivity of OVA/total amount of OVA released ${\times}100(%)$. ELISA method was an efficient tool to detect a protein denaturation during microencapsulation and the comparison of AR values resulted in more accurate evaluation for immunoreactivity of entrapped protein.

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