• Archives of Pharmacal Research
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• v.28 no.11
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• pp.1275-1281
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• 2005

The $G_{q/11}$ protein-coupled receptors, such as muscarinic (M1 & M3) receptors, have been shown to regulate the release of a soluble amyloid precursor protein (sAPP$\alpha$) produced from $\alpha$-secretase processing. However, there is no direct evidence for the precise characteristics of G proteins, and the signaling mechanism for the regulation of $G_{q/11}$ protein-coupled receptor mediated sAPP$\alpha$ release is not clearly understood. This study examined whether the muscarinic receptor-mediated release of sAPP$\alpha$ is directly regulated by $G\alpha_{q/11}$ proteins. The HEK293 cells were transiently cotransfected with muscarinic M3 receptors and a dominant-negative minigene construct of the G protein $\alpha$ subunit. The sAPP$\alpha$ release in the media was measured using an antibody specific for sAPP. The sAPP$\alpha$ release enhancement induced by muscarinic receptor stimulation was decreased by a $G_{q/11}$ minigene construct, whereas it was not blocked by a control minigene construct (the G$\alpha$ carboxy peptide in random order, G$\alpha_{q}$R) or $G\alpha_{j}$ constructs. This indicated a direct role of the $G\alpha_{q/11}$ protein in the regulation of muscarinic M3 receptor-mediated sAPP$\alpha$ release. We also investigated whether the transactivation of the epidermal growth factor receptor (EGFR) by a muscarinic agonist could regulate the sAPP$\alpha$ release in SH-SY5Y cells. Pretreatment of a specific EGFR kinase inhibitor, tyrophostin AG1478 (250 nM), blocked the EGF-stimulated sAPP$\alpha$ release, but did not block the oxoM­stimulated sAPP$\alpha$ release. This demonstrated that the transactivation of the EGFR by muscarinic receptor activation was not involved in the muscarinic receptor-mediated sAPP$\alpha$ release.

• Archives of Pharmacal Research
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• v.18 no.3
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• pp.183-188
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• 1995

Polymeric reinforcement and coatings of alginate beads were carried out to control the release rate of drug from alginate beads. A poorly water-soluble ibuprofen (IPF) was selected as a model drug. A commercially available $Eudragit^{\circledR}$ RS100 was also used as a polymer. Effects of polymeric contents, the presence of plasticizers and amount of drug loading on the release rate of drug were investigated. The release rate of drug from alginate beads in the simulated gastric fluid did not occur within 2 h but released immediately when dissolution media were switched to the simulated intestinal fluid. No significant difference of release rate from polymer-reinforced alginate bead without plasticizers was observed when compared to plain (simple) beads. However, the release rate of drug from polymer-reinforced alginate beads was further sustained and retarded when aluminium tristearate (AT) as a plasticizer was added to polymer. However, polyethylene glycol 400 (PEG400) did not change the release rate of drug from alginate beads although PEG400 was used to improve dispersion of polymer and sodium alginate, and plasticize $Eudragit^{\circledR}$ RS100 polymer. The presence of plasticizer was crucial to reinforce alginate gel matrices using a polymer. As the amount of drug loading increased, the release rate of drug increased as a result of decreasing effects of polymer contents in matrices. The significantly sustained release of drug from polymer-coated alginate beads occurred as the amount of polymer increased because the thickness of coated membrane increased so that cracks and pores of the outer surface of alginate beads could be reduced. The sustained and retarded action of polymer-reinforced and coated beads may result from the disturbance of swelling and erosion (disintegration) of alginate beads. From these findings, polymeric-reinforcement and coatings of alginate gel beads can provide an advanced delivery system by retarding the release rate of various drugs.

• Journal of the Korean Wood Science and Technology
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• v.8 no.2
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• pp.1-5
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• 1980

This study is carried out to determine the scavenging effect of resorcinol added into the urea formaldehyde resin on the formaldehyde release of plywood, as the preliminary study of using the phenolic substances. The method for formaldehyde determination used in this report is the improved chromotropic acid determination. The results are summarized as follows: 1. Resorcinol added into the urea formaldehyde adhesive acts as a good scavenger. 4 percent of resorcinol reduced the formaldehyde release to less than half content. 2. Adding resorcinol gave better glue shear strength than that of control, showing the peak of the shear strength, at 2 percent and decreased to the same strength as control along its content of 4 percent. 3. Moisture content of air dried plywood met the standard very well.

• Journal of Korea Water Resources Association
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• v.33 no.6
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• pp.745-755
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• 2000

The dam operation system of KOWACO for flood control doesn't have capability to account for the downstream hydrologic conditions and any feasible index to decide the pre-release from the forecasted rainfall and inflow. In this study, a dam operation model for flood control was developed to account for the flood flow condition of its downstream to give users the dam release schedules. Application test of EV ROM to Keum River showed that EV ROM is superior to the Rigid ROM and Technical ROM which are currently used by KOWACO. EV ROM developed in this study provides a release schedule accounting for the cumulative lateral flow hydrograph at the downstream control points where the discharge does not depend only on the dam operation. but also on lateral inflow from the tributaries. In order to reduce the peak discharge at the control points, it suggests the preliminary release during the early rising phase of the predicted hydrograph, holding the flood flow inside the dam during a peak phase, and afterward resuming the release. Three case studies of flood control by the operation of Daechung Multipurpose Dam in Geum River Basin show that the EV ROM is superior to the Rigid ROM and Technical ROM. This must be due to its nature to account for the downstream flow condition as well as the inflow and water level of the dam. It was also conceived that further case studies of EV ROM and more accurate rainfall prediction would improve the dam operation for flood control.ontrol.

• The Korean Journal of Physiology and Pharmacology
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• v.1 no.5
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• pp.573-580
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• 1997

In this report, the inhibitory action of eupatilin was investgated by using leukotriene $D_4$ in the human neutrophils and Kato III cells (Gastric adenoma cells as a substitute for gastric mucosal cells) stimulated by Helicobacter pylori. Leukotriene $D_4$ ($LTD_4$) was released from both neutrophils and Kato III cells when these cells were incubated with H. pylori. The release of $LTD_4$ increased time-dependently and the maximum release of $LTD_4$ was $2.3{\sim}2.5$ pmol. But in the presence of eupatilin, the release of $LTD_4$ from these cells was inhibited in a dose-dependent manner. In the neutrophils, the release of $LTD_4$ was suppressed to 70% and 50% of the control levels when neutrophils was incubated with 0.01 and 0.1 mM of eupatilin. In the Kato III cells, the release of $LTD_4$ was suppressed to 59% and 27% of the control levels by adding 0.01 and 0.1 mM of eupatilin. We estimated the intracellular $Ca^{2+}$ levels when Kato III cells and neutrophils were stimulated by H. pylori using $^{45}Ca$. But the suppressive effect of eupatilin on $Ca^{2+}$ influx into these cells was not significant. We also obtained the results that H. pylori induced $Ca^{2+}$ influx into these cells by confocal microscopy, however there was no differences in the dose level of eupatilin. These results were confirmed by 1H Nuclear Magnetic Resonance(NMR) spectroscopy. The NMR patterns of eupatilin in the absence of $Ca^{2+}$ was changed compare with when $Ca^{2+}$ was present, but its effect was not strong.

• The Korean Journal of Physiology
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• v.25 no.1
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• pp.27-35
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• 1991

Generally, it has been known that cholecystokinin (CCK) release into the plasma is under cholinergic control, but secretin release is not. Thus in anesthetized dogs we studied the effect of atropine $(50\;{\mu}g/kg\;followed\;by\;50\;{\mu}g/kg/hr)$ on pancreatic secretion and plasma concentrations of bioactive CCK and immunoreactive secretin in response to intraduodenal perfusion of sodium oleate (1, 3 and 9 mmol/hr). The volume, protein output and bicarbonate output of the secretion were increased by sodium cleats and this oleate-induced secretion was decreased significantly by atropine administration. However the increased plasma CCK and secretin levels by sodium oleate were not changed by atropine. These results indicate that atropine suppressed sodium oleate-induced pancreatic secretion through inhibiting cholinergic mechanism directly rather than decreasing the release of pancreatic secretory hormones. In another set of experiments, bilateral cervical vagi were stimulated electrically to observe the changes of pancreatic secretion and the above two plasma hormone levels in the presence or absence of atropine. In the vagally stimulated dogs, the volume, protein output and bicarbonate output of the pancreatic secretion were increased significantly. Both plasma secretin and CCK were concomitantly released significantly by vagal stimulation. Atropine significantly depressed the pancreatic secretory response as well as the release of these two pancreatic secretory hormones. Therefore, we conclude that in the presence of atropine the depressed pancreatic response to vagal stimulation is at least, in part, due to decreased release of endogenous CCK and secretin. In the vagally stimulated animals, however, the involvement of direct cholinergic influence on pancreatic exocrine gland remains to be answered.

• The Journal of Korean Physical Therapy
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• v.31 no.1
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• pp.24-30
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• 2019

Purpose: This study compared the effectiveness of three methods, fascial distortion model (FDM), myofascial release (MFR), self-myofascial release (SMR), on the neck range of motion and pain. Methods: In this study, the collected data were processed statistically using SPSS version 22.0 for Windows. Descriptive statistics were used to analyze the general characteristics of the subjects. Repeated measure ANOVA was conducted to analyze the range of motion of the neck of the group and VAS, and Contras was used to see the difference in significance over time. One-way ANOVA was used to compare the differences among the groups and a post-hoc test was used. The significance level (${\alpha}$) was 0.05. Results: In the range of motion, the flexion and extension of the neck, right rotation, and left rotation were significantly different in the SMR, FDM, and MFR groups. The right lateral flexion showed significant differences in the FDM, MFR, and SMR groups. The VAS was similar in the groups at 2 and 4 weeks, but there was a significant difference among the FDM, MFR, and SMR groups at 6 weeks. Conclusion: In this study, MFR and MSR as well as FDM were effective in controlling the range of motion and pain control of the neck. Further studies will be needed to determine the effects of long-lasting treatments other than pain control. These studies and the present study will be used as a basis for ongoing research into the duration and method of application for musculoskeletal therapies.

• Advances in nano research
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• v.12 no.6
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• pp.629-638
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• 2022

This work shows the impregnation of scents using a graphene sponge (GS). This was functionalized by the modified Hummers method, pursuing to add different functional groups. It is proposed to achieve the release and seek to control it through electrical potential applied to the graphene sponge with essential oils. The graphene sponge was functionalized and steeped with two kinds of oil. The electrochemical study demonstrates the variation in the electrochemical behaviour of the functionalized graphene sponge without and impregnated with oil. The release of the oil and its aromatic scents was carried out by applying an electrical potential of 30 V, with a release rate of 1.86 mg/min. The heating of the sample that causes the release of oil, associated with the electrical resistance of the system, reaches temperatures of about 150℃. The essential oils, graphene sponge, surfactant, graphene sponge with essential oils, graphene sponge recuperated after applying electric potential, graphene sponge recovered by temperature and dipropylene glycol (DPG) were characterized using Fourier transformed infrared spectroscopy (FTIR), digital microscopy, and x-ray photoelectron spectroscopy (XPS).

• Journal of East-West Nursing Research
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• v.8 no.1
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• pp.63-72
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• 2003

Purpose: The Purpose of this study was to test the effects of the Muscles Along Meridians Release Therapy on the function of upper limb as a means of nursing intervention. Method: The design used for this study was quasi - experimental with a nonequivalent control group pretest - post test design. The subjects were 40 stroke patients who were admitted in K oriental medical center of K University. This study was carried out from 6, May to 18, October, 2003. The experimental group (21) and the control group (19) were assigned by means of Participation order. The experimental group took Muscles Along Meridians Release Therapy on affected upper limb for 3 minutes daily for 2 weeks. Outcome were assessed by Modified Ashworth Scale, VAS, Fugl - Meyer score and goniometer. Data were analysed by SPSS PC. Result: After 2 weeks of treatment, function of affected upper limb, elbow joint spasticity were significantly better than control group, but, there was no significant difference in pain between experimental group and control group. Conclusion: The above results state that the Muscles Along Meridians Released Therapy could be an effective intervention for improving upper limb function and elbow joint spasticity of stroke patients.

• Journal of Environmental Science International
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• v.28 no.5
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• pp.511-520
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• 2019

This study was investigated to improve the phosphorus release and water quality by transformation of sedimentary P fraction for application of $CaO_2$. For the experiment, 0.5% (w/w) of $CaO_2$ was homogenized in the sediment and incubated with the control for 20 days. The analytical results showed that pH increased with $CaO_2$ and redox potential (ORP) was improved in the sediment of the reactor. The growth rate of chlorophyll-a was lower in the $CaO_2$ reactor and Dissolved Oxygen (DO) of overlying water maintained higher than that of the control. Total phosphorus (T-P) concentration in the overlying water increased from the initial concentration to 0.304mg/L in the control at 20 days. The reactor of $CaO_2$ was lowered by 29.3%. Ex-P, Fe-P and Ca-P in sediment P fraction were increased with the $CaO_2$. The formation of bound Fe-P and Ca-P in the sediments seemed to control the release of P by removing the Soluble Reactive Phosphorus (SRP) presented in the pore water. From the result, this indicated that the reduction of P release from the sediments seems to be effective in suppressing the eutrophication of P and improving the oxygen condition in the water quality with the application of $CaO_2$.