• Tuberculosis and Respiratory Diseases
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• v.85 no.2
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• pp.147-154
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• 2022

Background: The expression of calcium signaling pathway molecules is altered in various carcinomas, which are related to the proliferation and altered characteristics of cancer cells. However, changes in calcium signaling in anti-cancer drug-resistant cells (bearing a T790M mutation in epidermal growth factor receptor [EGFR]) remain unclear. Methods: Afatinib-mediated changes in the level of store-operated Ca²⁺ entry (SOCE)-related proteins and intracellular Ca²⁺ level in non-small cell lung cancer cells with T790M mutation in the EGFR gene were analyzed using western blot and ratiometric assays, respectively. Afatinib-mediated autophagic flux was evaluated by measuring the cleavage of LC3B-II. Flow cytometry and cell proliferation assays were conducted to assess cell apoptosis and proliferation. Results: The levels of SOCE-mediating proteins (ORAI calcium release-activated calcium modulator 1 [ORAI1], stromal interaction molecule 1 [STIM1], and sarco/endoplasmic reticulum Ca²⁺ ATPase [SERCA2]) decreased after afatinib treatment in non-small cell lung cancer cells, whereas the levels of SOCE-related proteins did not change in gefitinib-resistant non-small cell lung cancer cells (PC-9/GR; bearing a T790M mutation in EGFR). Notably, the expression level of SOCE-related proteins in PC-9/GR cells was reduced also responding to afatinib in the absence of extracellular Ca²⁺. Moreover, extracellular Ca²⁺ influx through the SOCE was significantly reduced in PC-9 cells pre-treated with afatinib than in the control group. Additionally, afatinib was found to decrease the level of SOCE-related proteins through autophagic degradation, and the proliferation of PC-9GR cells was significantly inhibited by a lack of extracellular Ca²⁺. Conclusion: Extracellular Ca²⁺ plays important role in afatinib-mediated autophagic degradation of SOCE-related proteins in cells with T790M mutation in the EGFR gene and extracellular Ca²⁺ is essential for determining anti-cancer drug efficacy.

• The Journal of Korean Medicine
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• v.22 no.4
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• pp.10-21
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• 2001

Objective : To investigate the effect between Fructus Immaturus Ponciri (FIP, the immature fruit of Poncirus trifoliata) and Fructus Ponciri (FP, the ripe fruit of Poncirus trifoliata) on mast cell-mediated immediate-type allergic reactions. Methods : We performed anaphylactic reaction, histamine release, cAMP, $TNF-{\alpha}$, IgE. Results : The aqueous extract of FIP dose-dependently inhibited systemic and local allergic reaction was induced by compound 48/80 or anti-dinitrophenyl (DNP) IgE in a murine model. FIP also significantly inhibited mast cell-dependent ear swelling response induced by topical application of compound 48/80. When mice were orally pretreated with FIP, the plasma histamine levels were reduced in a dose-dependent manner. FIP dose-dependently inhibited histamine release from the rat peritoneal mast cells (RPMCs) was activated by compound 48/80 or anti-DNP IgE. The level of cAMP in RPMCs, when FIP was added, increased compared with that of a normal or control. In addition, FIP had a significant inhibitory effect on anti-DNP IgE-induced tumor necrosis factor-a ($TNF-{\alpha}$) production from the RPMCs and IgE produced by lipopolysaccharide-stimulated murine whole spleen cells or U266B1 as human IgE-bearing B cells. However, FP showed the lower inhibition rate than those of FIP in above all allergic reactions. Conclusion : These data have important implications for our understanding of the clinical effects of FIP and FP on allergic diseases, and FIP is more effective than FP on the allergic reaction.

• The KSFM Journal of Fluid Machinery
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• v.5 no.4 s.17
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• pp.26-31
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• 2002

The heat produced by the fission in the fuel of HANARO, 30 MW of research reactor, was transferred from the primary cooling water to the secondary cooling water through heat exchangers. The secondary cooling water absorbing the heat was circulated by secondary cooling pumps and cooled through 33 MW of cooling tower. Each capacity of the three secondary cooling pumps was fifty percent ($50\%$) of full load. The two pumps were normally operated and the other pump was standby. One of the secondary cooling pumps has often made troubles by high vibration. To release these troubles the pump shaft has been re-aligned, the pump bearing has been replaced with new one, the shaft sleeve has been replaced with new one, the shaft and the impeller have been re-balanced representatively and/or the vibration of motor has been tested by disconnecting the shaft of pump. But the high vibration of pump cannot be cleared. We find out the weight balance trouble of the assembly in which the impeller is installed in the shaft. After clearing the trouble, the high vibration is relieved and the pump is operated smoothly. In this paper, the trouble solution shooting method of secondary cooling pump is described including the reason of high vibration

• Proceedings of the Korea Concrete Institute Conference
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• 2008.11a
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• pp.645-648
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• 2008

The expansion joint is an important part of the bridge, but the failure is occurred on the non-shrinkage concrete which is connected to the slab of the bridge and the expansion joint, and the other problem is the release of anchors in expansion joint due to the impact and vibration during the driven car on the bridge, especially an overloaded car. In this study, to overcome the failure of non-shrinkage of concrete, high impact resisting mortar is developed. The high impact resisting mortar shall be a polyurethane material compounded with an aggregate system to develop excellent flexibility characteristics, high load bearing capacity.

• 유체기계공업학회:학술대회논문집
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• 2001.11a
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• pp.197-202
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• 2001

The heat produced by the fission in the fuel of HANARO, 30 MW of research reactor, was transferred from the primary cooling water to the secondary cooling water through heat exchangers. The secondary cooling water absorbed the heat was circulated by secondary cooling pumps and cooled through 33 MW of cooling tower. Each capacity of the three secondary cooling pumps was fifty percent ($50\%$) of full load. The two pumps were normally operated and the other pump was standby. One of the secondary cooling pumps has often get troubles by high vibration. To release these troubles the pump shaft has been re-aligned, the pump bearing has been replaced with new one, the shaft sleeve has been replaced with new one, the shaft and the impeller have been re-weight balanced representatively or the vibration of motor has been tested by disconnecting the shaft of pump. But the high vibration of pump cannot be cleared. We find out the weight balance trouble of the assembly that the impeller is installed in the shaft. After clearing the trouble, the high vibration is released and the pump is operated with smooth. In this paper the trouble solution of secondary cooling pump is described including the reason of high vibration.

• Natural Product Sciences
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• v.2 no.1
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• pp.29-36
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• 1996

This study was to evaluate the antitumor and immunopotentiation effects of Manda Enzyme (ME). Oral administration of ME (0.2ml/mouse) to tumor bearing mice significantly prolonged survival rate compared to the control group with the prolongation ratio of 40%. The inhibition ratios for the first and the second experiments were 51.8% and 26.4%, respectively. Only the spleen index was significantly increased in the MEF-treated group, but not in the control group. Gamma globulin level of the MEF-treated group was elevated when mice were injected with sarcoma-180 cells on the left groin. Activities of natural killer (NK) and lymphokineactivated killer (LAK) cells were observed by $^{51}Cr-release$ method. Activities of NK cell against YAC-1 cells were significantly increased in the MEF treated group. And LAK cell activities against P815 cells were also significantly increased in the experimental group. These observations, therefore, suggest that ME may have an anticancer effect and immunopotentiating effect in vivo.

• The Plant Pathology Journal
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• v.16 no.3
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• pp.147-150
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• 2000

Sporulation patterns of rice blast fungus were studied at relatively later stages of leaf blast and neck blast seasons in Icheon, Korea. This experiment was done by detaching lesion-bearing leaves and panicle bases. The number of conidia remaining on the leaf blast lesions of different cultivars from Jul 20 to Jul 23 ranged from 3,640 to 82,740 spores. More conidia were observed on the adaxial surface because they were released from abaxial surface. After heading, sporulation was observed from the lesions on the flag leaves but the number of spores was less than in the late July. Detached panicle bases or uppermost internodes infected by Pyricularia grisea produced abundant amount of conidia. Among these panicle bases, 30.1 mm size lesion recorded the highest count of 244,560 spores. When we compared the sporulation amount using the KY-type spore trap, more conidia were recorded from intact lesions than from the lesions which removed conidia and conidiophore The ratio of conidia release against total sporulation ranged from 20.5%-25.0% for leaf blast and 8.2%-25.3% in the neck blast. Effective inoculum potential was also discussed.

• Journal of the Architectural Institute of Korea Structure & Construction
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• v.36 no.4
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• pp.135-142
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• 2020

The new method for evaluating the displacement tolerance of sky-bridges with pin-roller type supports was proposed considering both return period of phase difference between connected buildings and geometrical characteristics of skybridge. Because displacement tolerance is relative value, which is most affected by the phase difference of the connected buildings, the dynamic response of these building with time history analysis should be evaluated. However, the initial phase could not be specified, so the result of displacement tolerance would be varied with respect to initial value. Thus, the tolerance can be reasonably evaluated SRSS calculation with design displacements based on statistical approach and of each building. In addition, the geometrical characteristics of sky-bridge should be considered because the transverse displacement of sky-bridge span causes the shear deformation of the bridge and longitudinal displacement tolerance cannot release the shear deformation. Therefore, the some pin-end support in sky-bridge should have longitudinal displacement tolerance to accommodate the shear deformation. By resolving this shear deformation, it is possible not only to accommodate transverse displacement, but also to avoid the complicated joint details such as both pot bearing and guided supports with shear key.

• Journal of the Mineralogical Society of Korea
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• v.15 no.3
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• pp.183-194
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• 2002

Biotite and its weathering Products in the weathering Profile of Andong granite were examined using X-ray diffraction, chemical analysis, and electron microscopy. Major weathering product of biotite was oxidized biotite, which is decomposed into kaolinite in the upper part. Discrete vermiculite or hydrobiotite was not detected although minor vermiculite (5%) was randomly interstratified with oxidized biotite. Excess positive charge induced by iron oxidation was balanced by release of Fe (16%) and Mg (12%) from octahedral site and K (13%) from interlayer site. After slight chemical and structural modification induced by iron oxidation, oxidized biotite persists through the weathering profiles with partial decomposition in the upper part of the profile. Formation environments and dissolution experiments of oxidized biotite highly resistant to weathering are required to understand the elemental behavior in the surface environments on the biotite-bearing bedrocks.

• Journal of Korean Foot and Ankle Society
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• v.14 no.2
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• pp.130-134
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• 2010

Purpose: The aim of this study was to evaluate the result of combined first metatarsal and calcaneal osteotomy for static cavovarus deformity of the foot. Materials and Methods: We performed a dorsal closing wedge $1^{st}$ metatarsal osteotomy and a lateral and upward displacement calcaneal osteotomy for 9 patients, 12 feet (6 male and 3 female). The mean age at the time of operation was 37 years and the mean followup period was 27 months. The causes of deformity were 2 poliomyelitis, 1 cerebral palsy, 1 Charcot-Marie-Tooth disease and 5 idiopathic type. Five lateral ligament reconstructions of the ankle and six percutaneous Achilles tendon lengthenings were added. The surgical results in terms of pain, function and alignment of the foot were evaluated by means of AOFAS ankle-hindfoot score and talo-$1^{st}$ metatarsal, calcaneus-$1^{st}$ metatarsal and calcaneal pitch angles were checked with weight bearing radiographs in lateral projection. Results: Talo-$1^{st}$ metatarsal and calcaneal pitch angles were reduced from the mean preoperative values of $21^{\circ}$ and $25^{\circ}$ to $12^{\circ}$ and $19^{\circ}$, respectively, at last followup. Also, calcaneus-$1^{st}$ metatarsal angle was increased from the mean $114^{\circ}$ to $114^{\circ}$. The mean AOFAS score was improved from 44.5 points preoperatively to 89.2 points at followup. There were 1 metatarso-cueiform joint nonunion, 1 sural nerve injury and 3 remaining symptomatic claw toes. Conclusion: Combined first metatarsal and calcaneal osteotomy appears to be an effective procedure for the treatment of adult static cavovarus foot.