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Distribution and Ecology of Marsh Clam in Gyeongsangbuk-do II. Reproductive Cycle and Larval Development of the Corbicula japonica (경상북도 재첩자원 분포 및 생태 조사 II. 일본재첩 Corbicula japonica의 생식주기 및 유생발생)

  • 변경숙;정의영
    • The Korean Journal of Malacology
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    • v.17 no.1
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    • pp.45-55
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    • 2001
  • Gametogenes, reproductive cycle, first sexual maturity(biological minimum size), sex ratio and larval development of the marsh clam Corbicula japonica were investigated monthly by histological observations. Samples were collected in brackish water of Gokgang stream, Kyungsangbuk-Do, Korea, from August 1997 to July 1998. Sexuality of Corbicula japonica is dioecious and the species are an oviparous clam. The gonads are irregularly arranged from the sub-region of mid-intestinal gland in visceral cavity to reticular connective tissue of foot. The ovary is composed of a number of ovarian sac which are branched arborescent. Oogonia actively proliferate along the germinal epithelium of ovarian sac, in which young oocytes are growing. The testis is composed of a number of testicular tubules, and the epithelium of the tubule has function of germinal epithelium, along which spermatogonia actively proliferate. A great number of undifferentiated mesenchymal tissue and eosinophilic granular cells are abundantly distributed between developing oocytes and spermatocytes in the early developmental stages. With the further development of the ovary and testis these tissue and cells gradually disappear. Then the undifferentiated mesenchymal tissue and eosinophilic granular cells are considered to be related to the growing of the oocytes and spermatocytes. The spawning period is from July to September, and the main spawning occur between July and August when seawater temperatures reach above 22$^{\circ}C$. The reproductive cycle of this species can be divided into five successive stages; early active (February to April), late active (May to July), ripe (June to September), partially spawned (July to September), degenerative (September to October) and resting stage (October to February). Percentages of first sexual maturity of female and male clams ranging in length from 10 mm to 12 mm are over 50% and 100% for clams over 16.0 mm in shell length. Fertilized eggs or Corbicula japonica were 80-90 ${\mu}{\textrm}{m}$ in diameter. In the early embryonic development of C. japonica, the appearance of polar body, trochophore and D-shaped veliger were observed around 40 min., 27 hours and 4 days after spawning, respectively, at a water temperature of 26.5-28.$0^{\circ}C$. The size of larvae of early umbo stage was about 185-210 ${\mu}{\textrm}{m}$ in shell length, 160-180 ${\mu}{\textrm}{m}$ in shell height around 7 days after fertilization. The correlation of relative growth between the culture day (D) and shell length (SL) was expressed by the following simple formula from D-shaped veliger to metamorphosing stage; SL = 13.300D + 209.36($r^2$= 0.9078).

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A Methodology of Ship Detection Using High-Resolution Satellite Optical Image (고해상도 광학 인공위성 영상을 활용한 선박탐지 방법)

  • Park, Jae-Jin;Oh, Sangwoo;Park, Kyung-Ae;Lee, Min-Sun;Jang, Jae-Cheol;Lee, Moonjin
    • Journal of the Korean earth science society
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    • v.39 no.3
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    • pp.241-249
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    • 2018
  • As the international trade increases, vessel traffics around the Korean Peninsula are also increasing. Maritime accidents hence take place more frequently in the southern coast of Korea where many big and small ports are located. Accidents involving ship collision and sinking result in a substantial human and material damage as well as the marine environmental pollution. Therefore, it is necessary to locate the ships quickly when such accidents occur. In this study, we suggest a new ship detection index by comparing and analyzing the reflectivity of each channel of the Korea MultiPurpose SATellite-2 (KOMPSAT-2) images of the area around the Gwangyang Bay. A threshold value of 0.1 is set based on a histogram analysis, and all vessels are detected when compared with RGB composite images. After selecting a relatively large ship as a representative sample, the distribution of spatial reflectivity around the ship is studied. Uniform shadows are detected on the northwest side of the vessel. This indicates that the sun is in the southeast, the azimuth of the actual satellite image is $144.80^{\circ}$, and the azimuth angle of the sun can be estimated using the shadow position. The reflectivity of the shadows is 0.005 lower than the surrounding sea and ship. The shadow height varies with the position of the bow and the stern, perhaps due to the relative heights of the ship deck and the structure. The results of this study can help search technology for missing vessels using optical satellite images in the event of a marine accident around the Korean Peninsula.

Studies on the Morphological, Anatomical and Physiological Characters in Populus alba × glandulosa and its Parent (Populus alba × glandulosa 와 그의 양친종(両親種)의 형태(形態), 해부학(解剖學), 생리학적(生理学的) 연구(硏究))

  • Kim, Chung Suk;Chon, Sang Keun;Hwang, Jin Sung
    • Journal of Korean Society of Forest Science
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    • v.49 no.1
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    • pp.11-31
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    • 1980
  • The morphological, anatomical and physiological traits were eximined for Populus alba ${\times}$ glandulosa which is an important planting species in Korea. The results obtained are as follows: 1. External characters in the leaf shape and chaff shape in the catkin were inherited as incomplete dominance but nectar gland was inherited as dominance. 2. Among the 15 selected clones, 9 clones were male, 2 clones female and 2 clones monoecious. 3. There were well-developed cork layers and bast fiber bundles in the bark. 4. Primordial leaves composed of 3 layers of cells and those undifferentiated into palisade and spongy parenchymas differed in its origin. 5. Leaf scare consisted of two kinds of tissues; one is connected to vascular bundle and the other not to vascular bundle. Tissues which had been connected to vascular bundle were isolated with only 2 or 3 layers of cork cells from the outside. 6. There was complicated arrangement in the vascular bundle of petioles. 7. Growth of the hybrid was sensitively influenced by external temperature, day-length and amount of light. In particular, it was apparent in height growth. 8. Flatness, loam soils and a $60{\times}60cm$ spacing might be best factors for the growth of P. alba ${\times}$ glandulosa. 9. The rooting of 15 clones was dependant upon external factors. 10. The growth of P. alba ${\times}$ glandulosa was best at around 80% of soil moisture content on the basis of plot water capacity. 11. Temperature difference between inside and outside stems below 100cm during the winter was the greatest at the south among seasons and among directions. 12. The sap movement was markedly influenced by air temperature, relative humidity in forest stand and moisture content in stem. 13. Total sugars in the cortex changed with season but did not differ in the dircetion of the stem. 14. Isoperoxidase variations in the leaf were different among 15 clones. Thus, it may be useful as a criterium for clonal identification. 15. The rate of soil moisture content decreased at a rapid slope was faster than that at a slow slope. Poor growth of P. alba ${\times}$ glandulosa at the slope was probably due to depletion of soil moisture.

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Growth and Tissue Nutrient Responses of Fraxinus rhynchophylla, Fraxinus mandshurica, Pinus koraiensis, and Abies holophylla Seedlings Fertilized with Nitrogen, Phosphorus, and Potassium at a Nursery Culture (묘포에서 질소, 인, 칼륨 비료주기가 물푸레나무, 들메나무, 잣나무, 전나무의 생장 및 양분에 미치는 영향)

  • Park, Byung-Bae;Byun, Jae-Kyung;Kim, Woo-Sung;Sung, Joo-Han
    • Journal of Korean Society of Forest Science
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    • v.99 no.1
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    • pp.85-95
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    • 2010
  • The purpose of this study was to quantitatively measure both growth performances and nutrient responses of Fraxinus rhynchophylla, Fraxinus mandshurica, Pinus koraiensis, and Abies holophylla seedlings, which are commercially planted in Korea, to nitrogen, phosphorus, and potassium fertilization. We used Dickson's quality index (QI) to compare growth performances and vector diagnosis to interpret nutrient status. Nitrogen fertilization increased more height and root collar diameter growth in F. rhynchophylla and F. mandshurica relative to no fertilization treatment. The QI of F. rhynchophylla and F. mandshurica was the highest on N treatment, but there were no significant differences between treatments for P. koraiensis and A. holophylla. Nitrogen fertilization increased total dry weight by 43, 41, 26, -9% for F. rhynchophylla, F. mandshurica, P. koraiensis and A. holophylla, respectively. In F. rhynchophylla, N fertilization increased N contents with similar N concentrations ("sufficiency"), decreased both P concentrations and P contents ("antagonism"), and decreased K contents with similar K concentrations ("toxic accumulation"). In P. koraiensis, N fertilization decreased N, P, and K concentrations because of more dry weight increases compared to uptaken contents ("dilution"), but N fertilization decreased N, P, and K contents with similar N, P, and K concentrations ("toxic accumulation"). In the light of quality index and vector diagnosis, F. rhynchophylla and F. mandshurica seedlings treated with N fertilization would have high field performance.

Study on hydroxy fatty acid contents changes and physiological responses under abiotic stresses in transgenic Camelina

  • Kim, Hyun-Sung;Lee, Hyun-Sook;Lim, Hyun-Gyu;Park, Won;Kim, Hyun-Uk;Lee, Kyeong-Ryeol;Ahn, Sung-Ju
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2017.06a
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    • pp.191-191
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    • 2017
  • Hydroxy fatty acid (HFA) is an important industrial resource that known to be extracted from seeds of Castor or Lesquerella. However, mass production of HFA from those crops are difficult because of their behavior or life cycle. In this study, we applied HFA synthesis related gene FAH12, RcPDAT1, RcLPCAT, RcDGAT2, and RcPDCT on bioenergy crop Camelina sativa. Furthermore, we determined NaCl or cold stress tolerance changes of transgenic Camelina. RcFAH12, RcPDAT1, RcLPCAT, RcDGAT2, and RcPDCT genes were cloned into multigene expression vector which is engineered with seed specific promoter of FAE1 or Napin. Combination of HFA genes multi-expression vector constructs were divided into Set3 (RcFAH12, RcPDAT1-2, RcLPCAT), Set4 (RcFAH12, RcDGAT2, RCPDAT1-2, RcLPCAT), and Set5 (RcFAH12, RcDGAT2, RCPDAT1-2, RcLPCAT, RcPDCT). Transgenic HFA synthesis Camelina plants were generated using agrobacterium-mediated vacuum infiltration system. Results of fatty acid composition of T1 transgenic Camelina seeds analyzed by GC-MS showed 9.5, 9.0, and 13.6 % of HFA contents in Set3#6, Set4#8, and Set5#10, respectively. Therefore, seeds of T2 generation were harvest from Set5#10 which is shown highest HFA contents, and, 17.7, 8.1 and 10.5 % of HFA contents were determined in Set5#10-5, Set5#10-8, and Set#10-10, respectively. However, 7.7% of C18:2 and 22.3 % of C18:3 among unsaturated fatty acids were decreased in Set5#10-5 than WT. Meanwhile, we confirmed abiotic stress responses in T2 transgenic Camelina Set5#10-5 and Set5#10-10 under 0, 100, 150, and 200 mM NaCl or 25, 15, and $10^{\circ}C$ temperature for 5 weeks. Both Set5#10-5 and Set5#10-10 showed lower growth in height than WT in control and NaCl condition. Growth of leaf length and width were similar in WT and Set5#10-10 but lower in Set5#10-5 under NaCl stress. Number of opened flowers showed that both transgenic Camelina were lower than WT under normal condition. But, WT and Set5#10-10 showed similar opened flower number in 100 and 200 mM NaCl. In cold stress, 15 and $10^{\circ}C$ treatment for 5 weeks did not showed significant changes in between WT and both transgenic lines even they showed different growth rate in control condition. Taken together, growth and development are delayed by expression of exogenous HFA related genes in transgenic lines but relative abiotic stress sensitivity is similar with WT. In conclusion, reduced C18:2 or C18:3 fatty acid composition of seed by HFA synthesis is resulted from lack of resource supplement for development at seedling stage but it is not affect NaCl and cold stress tolerance.

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Elevated Temperature Treatment Induced Rice Growth and Changes of Carbon Content in Paddy Water and Soil (온도상승 환경 처리가 논토양과 용수에서 탄소량 변화와 벼 생육에 미치는 영향)

  • Hong, Sung-Chang;Hur, Seung-Oh;Choi, Soon-Kun;Choi, Dong-Ho;Jang, Eun-Suk
    • Korean Journal of Environmental Agriculture
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    • v.37 no.1
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    • pp.15-20
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    • 2018
  • BACKGROUND: The global mean surface temperature change for the period of 2016~2035 relative to 1986~2005 is similar for the four representative concentration pathway (RCP)'s and will likely be in the range of $0.3^{\circ}C$ to $0.7^{\circ}C$. Climate change inducing higher temperature could affect not only crop growth and yield, but also dynamics of carbon in paddy field. METHODS AND RESULTS: This study was conducted to evaluate the effect of elevated temperature on the carbon dynamics in paddy soil and rice growth. In order to control the elevated temperatures, the experiments were set up as the small scale rectangular open top chambers (OTCs) of $1m(width){\times}1m(depth){\times}1m(height)$ (Type 1), $1 m(W){\times}1m(D){\times}1.2m(H)$ (Type 2), and $1m(W){\times}1m(D){\times}1.4m(H)$ (Type 3). The average temperatures of Type 1, Type 2, and Type 3 from July 15 to October 30 were higher than the ambient temperatures at $0.4^{\circ}C$, $0.5^{\circ}C$, and $0.9^{\circ}C$, respectively. For the experiment, Wagner's pots (1/2,000 area) were placed inside chambers. The pots were filled with loamy soil, and chemical fertilizer and organic compost were applied as recommended after soil test. The pots were flooded with agricultural water and rice (Shindongjin-byeo) was planted. It was observed that TOC (total organic carbon) of the water increased by the elevated temperatures and the trend continued until the late growth stage of the rice. Soil TOC contents were reduced by the elevated temperatures. C/N ratios of the rice plant decreased by the elevated temperature treatments. Thus, it was assumed that the elevated temperatures induced to decompose soil organic matter. Elevated temperatures significantly increased the culm length (P<0.01) and culm weight (P<0.05) of rice, but the number and weight of rice panicle did not showed significant differences. CONCLUSION: Based on the results, it was suggested that the elevated temperatures had an effect on changes of soil and water carbons under the possible future climate change environment.

Development of lumped model to analyze the hydrological effects landuse change (토지이용 변화에 따른 수문 특성의 변화를 추적하기 위한 Lumped모형의 개발)

  • Son, Ill
    • Journal of the Korean Geographical Society
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    • v.29 no.3
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    • pp.233-252
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    • 1994
  • One of major advantages of Lumped model is its ability to simulate extended flows. A further advantage is that it requires only conventional, readily available hydrological data (rainfall, evaporation and runoff). These two advantages commend the use of this type of model for the analysis of the hydrological effects of landuse change. Experimental Catchment(K11) of Kimakia site in Kenga experienced three phases of landuse change for sixteen and half years. The Institute of Hydrology offered the hydrological data from the catchment for this research. On basis of Blackie's(l972) 9-parameter model, a new model(R1131) was reorganized in consideration of the following aspects to reflect the hydrological characteristics of the catchment: 1) The evapotranspiration necessary for the landuse hydrology, 2) high permeable soils, 3) small catchment, 4) input option for initial soil moisture deficit, and 5) othel modules for water budget analysis. The new model is constructed as a 11-parameter, 3-storage, 1-input option model. Using a number of initial conditions, the model was optimized to the data of three landuse phases. The model efficiencies were 96.78%, 97.20%, 94.62% and the errors of total flow were -1.78%, -3.36%, -5.32%. The bias of the optimized models were tested by several techniques, The extended flows were simulated in the prediction mode using the optimized model and the data set of the whole series of experimental periods. They are used to analyse the change of daily high and low-flow caused by landuse change. The relative water use ratio of the clearing and seedling phase was 60.21%, but that of the next two phases were 81.23% and 83.78% respectively. The annual peak flows of second and third phase at a 1.5-year return period were decreased by 31.3% and 31.2% compared to that of the first phase. The annual peak flow at a 50-year return period in the second phase was an increase of only 4.8%, and that in the third phase was an increase of 12.9%. The annual minimum flow at a 1.5-year return period was decreased by 34.2% in the second phase, and 34.3% in the third phase. The changes in the annual minimum flows were decreased for the larger return periods; a 20.2% decrease in the second phase and 20.9% decrease in the third phase at a 50-year return period. From the results above, two aspects could be concluded. Firstly, the flow regime in Catchment K11 was changed due to the landuse conversion from the clearing and seedling phade to the intermediate stage of pine plantation. But, The flow regime was little affected after the pine trees reached a certain height. Secondly, the effects of the pine plantation on the daily high- and low-flow were reduced with the increase in flood size and the severity of drought.

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Abdominal Obesity in Relation to the Incidence of Type 2 Diabetes Mellitus and Impaired Fasting Glucose among some Korean Adults: A Retrospective Cohort Study (복부비만이 제2형 당뇨병 및 공복혈당장애 발생에 미치는 영향: 후향적 코호트 연구)

  • Ryu, Seung-Ho;Beck, Sung-Ho;Kim, Dong-Il;Suh, Byung-Seong;Kim, Woon-Sool;Sung, Ki-Chul;Chang, Yoo-Soo
    • Journal of Preventive Medicine and Public Health
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    • v.37 no.4
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    • pp.359-365
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    • 2004
  • Objectives : This study was performed to determine whether an increase in abdominal obesity is an independent risk factor for impaired fasting glucose and type 2 DM. Methods : Among 24,212 adults over 30 years who undertook comprehensive medical screening examinations from Jan to Dec 1999, in a university hospital in Seoul, a total of 11,183 subjects were selected who had no DM at baseline and who were followed up more than once by Dec 2002. The average follow up period was 2.4 (${\pm}0.5$) years. DM was defined as having a fasting glucose level $\geq$ 126mg/dl, and impaired fasting glucose as showing a fasting glucose level between 110 and 125 mg/dl. Body weight, height and waist circumference (WC) were simultaneously measured with blood sampling. The relative risks (RRs) for DM and impaired fasting glucose by WC were calculated using Cox proportional hazard model. Ageadjusted rates were estimated by direct standardization using a reference population of 2000 from 30 to 80 years. Results : The average age of the subjects was 41.7 (${\pm}7.0$) years; males 41.2 (${\pm}6.5$) and females 45.6 (${\pm}9.2$). RRs for type 2 DM by WC with the reference group of WC < 80cm were as follows: 2.66 (95%, CI $0.55{\sim}12.8$) for WC of $80{\sim}89cm$ in men, 5.92 (95%, CI $1.08{\sim}32.3$) for WC $\geq$ 90 cm in men, and 2.64 (95%, CI $0.23{\sim}29.8$) for WC of $80{\sim}89cm$ in females. RRs for impaired fasting glucose by WC were 3.03 (95%, CI $2.18{\sim}4.22$) for WC $80{\sim}89cm$ in men, 6.10 (95%, CI $4.25{\sim}8.75$) for WC $\geq$ 90cm in men, and 1.56 (95%, CI $0.43{\sim}5.67$) for WC $80{\sim}89cm$ in women, and 8.08 (95%, CI $2.22{\sim}29.4$) for WC $\geq$ 90cm in females. These results remained significant after adjustment for age, BMI and fasting glucose concentrations at baseline in both sexes. Annual increment of more than 1 cm in WC was associated with the development of DM and impaired fasting glucose independently of age, sex, BMI, or presence of abdominal obesity. Conclusion : In Korean adults, abdominal obesity increased the risk for the development of type 2 diabetes and impaired fasting glucose. This result supports many other prospective studies suggesting abdominal obesity as a risk factor for type 2 diabetes.

Ecological Characteristics of Bombus ignitus Smith in Korea (한국산 호박벌(Bombus ignitus Smith)의 생태적 특성)

  • ;;;;M. Bilinski
    • Korean journal of applied entomology
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    • v.38 no.2
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    • pp.101-107
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    • 1999
  • Queens of Korean native bumblebee species, Bombus ignitus were collected from the field in the spring of 1998 to investigate their ecological characteristics and reared under the laboratory conditions; 3 0 f 1$^{\circ}$C, 55 25% of relative humidity and darkness. As a result, 94.4% of the queens laid eggs in 6.00f 2.41 days after the field collection. The first worker and the first drone from the egg cell of the collected queens appeared in 18.90 f 1.16 and 68.96 f 3.94 days, respectively. In the colony foundation, 86.1% of the collected queens founded their colony, and 96.8% of them produced new queens in 71.14f 6.88 days. The life span of the colony-founded queen covered for 86.90f24.35 days, whereas that of non-colonized queen for 15.67 f 7.37 days. One colony sized as big as 987.82f 145.84 in number, composing of workers (188.79f 18.23), drones (691.10f 159.24) and queens (107.93 f47.48). Copulation lasting time of queen and drone took 23.00f 7.33 minutes in average. In a new queen emergence, there were two patterns; single peak type of new queen emergence in 12.31 k6.95 days and two peaks type of it in 74.00f 17.61 days. The number of eggs per egg cell of new queen averaged as 8.86f 4.00 grains and an egg period covered 3 days after oviposition. An egg cell sized as 7.15 f0.88 mm in a width, 7.79 f 1 .l 1 mm in a length and 3.33 f 0.23 mm in a height. An egg shaped as banana and sized as 1.22 f 0.09 mm in a width and as 3.5 1 f 0.21 mm in a length, weighing as 2.70 + 0.30 mg.

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Vegetation Structure of Vaccinium bracteatum Thunb. Habitat Area at A Valley of Southern Slope in Mt. Halla, Jeju Island (한라산 남사면 계곡 모새나무 자생지 일대의 식생구조)

  • Song, Jung-Min;Kim, Yunjung;Sun, Byung-Yun;Kim, Chul Hwan;Do, Seon-Gil;Song, Gwanpil
    • Korean Journal of Plant Resources
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    • v.26 no.5
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    • pp.589-596
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    • 2013
  • Vaccinium bracteatum Thunb. is an evergreen plant that belongs to the Genus Vaccinium distributed in Korea, and its fruit has been used for food by the common people and thus has a high possibility of commercialization. Therefore, the habitat environment and individual characteristics of V. bracteatum in Jeju Island were investigated to obtain the basic data for commercialization and preservation. The results showed that V. bracteatum is mostly distributed on the southern slope of Mt. Halla at an altitude of 100 to 500 m. V. bracteatum that occurred in the quadrat were total 30 individuals, which had an average height of 6.0 m, an average DBH of 7.8 cm, and an average number of trunk of 4.3. As for the community structure of the habitat, the average percentage of vegetation cover for the tree layer was 36%, while that for the subtree layer was 78%, which emphasized the importance of the subtree layer. The total number of species that occurred in the quadrat was 76. For the tree layer, total 10 species occurred, which include 2 species of evergreen coniferous trees, 3 species of evergreen broad-leaved trees, and 5 species of deciduous broad-leaved trees. For the subtree layer, total 24 species occurred, which include 1 species of evergreen coniferous tree, 11 species of evergreen broad-leaved trees, and 10 species of deciduous broad-leaved trees. Among these, Eurya japonica Thunb. was the species with a high relative contribution, which threatened V. bracteatum For the shrub layer, 27 species occurred including Cinnamomum japonicum Siebold, and for the herbaceous layer, 64 species occurred including Ardisia crenata Sims. The similarity between the habitats of V. bracteatum was very low, and it did not prefer a characteristic community. The V. bracteatum community around Shinrei stream was distributed on the surrounding area adjacent to the slope of the stream rather than the slope. There were a lot of basins, but the branches, which did not receive sunlight, had withered. It is thought that V. bracteatum would decline if the forest formation continues.