• 식물조직배양학회지
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• 제27권3호
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• pp.197-202
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• 2000

조직배양을 이용한 처녀치마 대량증식을 위한 적정배지, 생장조절제 및 농도구명을 위해 본 연구를 수행하였다. Shootb 및 roots분화의 경우 zeatin 3.0 mgι$^{-1}$을 첨가한 MS 배지에서 shoots및 roots 형성률이 각각 20, 30%로서 가장 양호한 것으로 나타났다. 1/2 MS배지의 경우 zeatin 1.0mgι$^{-1}$에서 shoots 형성률이 67 %, B5 배지에 있어서 zeatin 3.0 mgι$^{-1}$ 처리에서 33 %로 가장 양호한 것으로 나타났다. 배지에 따른 식물 생장조절제 처리에 따른 식물체 분화율은 MS 배지에서는 2,4-D 0.5 mgι$^{-1}$, 1/2 MS 배지에서 zeatin 1.0 mgι$^{-1}$ 및 B5 배지에서 reatin 3.0 mgι$^{-1}$에서 자장 가장 많은 100, 193, 280 및 310%의 분화된 식물체를 얻을 수 있었다. 전반적으로 야생화인 처녀치마의 잎 조직으로부터 multiple shoots을 유도하여 대량증식을 위한 목적으로는 B5 배지에 생장조절제인 zeatin 3.0 mgι$^{-1}$을 첨가시키는 것이 가장 효과적인 것으로 나타났다.

• Journal of Preventive Medicine and Public Health
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• 제30권3호
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• pp.555-566
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• 1997

This study was carried out to evaluate the cytotoxicity of rock wool fibers(RWFs) such as cell division disturbance, chromosomal and DNA damage, and mutagenicity using cultured cells. RWFs were the man made mineral fibers. In order to find the correlation between the cytotoxicity of RWFs and the phagocytic capacity of cells, the phagocytic processes were observed using scanning electron microscope. Cell division disturbance by RWFs was evaluated by the formation of multinucleated giant cells. The chromosomal damage was evaluated by the micronucleus formation. For the evaluation of oxidative DNA damage, 8-hydroxy-2'-deoxyguanosine (8-OH-dG) formation was measured utilizing calf thymus DNA. Mutagenicity was determined by the point mutation of HGPRT and the effect of RWFs on cell transformation was also observed. 1. Compared with the results of chrysotile, RWFs were no or little effect on the cell growth according to the results done by the tests of cell proliferation inhibition and relative plating efficiency. 2. The frequency of multinucleated giant cell formation was increased by the treatment of RWFs and it was dose-dependent. However, the effect of RWFs was weaker than that of chrysotile. 3. The number of micronuclei formed in the RWFs treated cells was between those of cells treated with chrysotile and those of untreated cells. 4. The 2 fold increase in the formation of 8-OH-dG in calf thymus DNA was observed in the cells treated with RWFs in the presence of $H_2O_2$. On the other hand, chrysotile had no effect on the 8-OH-dG formation. 5. RWFs had no effect on the HGPRT point mutation and cell transformation. These results showed that RWFs could induce chromosomal damage, cell division disturbance and oxidative DNA damage in the RWFs treated cells.

• 한국초지조사료학회지
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• 제15권2호
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• pp.118-123
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• 1995

This experiment was canied out to determine the effects of nitrogen (N) fertilization level (0, 70, 140, 280 and 420 k a a ) on the grass growth, dry matter (DM) production, numtive value, and botanical composition in reed canarygrass (Phalaris ancndinacea L.) pasture. \ulcornerhe cultivar used in this study was Palaton, and the grass was harvested at soiling-hay stage (3 times per year) in 1992 and 1993. As the N level was increased, the plant height, DM produdion, and crude protein (CP) content were significantly increased (P<0.05). When the N was applied at the levels of 0, 70, 140, 280, 420 kg, DM yields were 4,540, 5,316, 6,060, 7,923 and 9,960 kgha, and CP contents were 17.6, 17.4, 17.4, 18.4 and 19.8%, respectively. However, as the N level was increased, DM percentage, total digestible nutrients, and relative feed value were decreased, while the contents of neutral detergent fiber and acid detergent fiber, and bareland were continuously increased, particularly in the plot of N 420 kg fdzation. From the above results, it is suggested that reed canarygrass was one of the sensitive pasture grasses to N fertilizer, and the proper amount of N application was 280 kg/ha/yr for desirable production, nutritive value, and botanical composition in r e d conarygrass pasmre.

• 원예과학기술지
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• 제33권5호
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• pp.625-632
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• 2015

Drought is a severe abiotic stress that affects global crop production. A drought model was created for 'Toyonoka' Fragaria ${\times}$ ananassa, and the effects of drought stress on contents of proline, sugars, and antioxidant enzyme activities were investigated. Strawberry transplants with identical growth were chosen for the experiments and the randomized design included four replications (10 plants per block). The experimental sets differed in the moisture level of the culture medium relative to the range of moisture content as follows: control, 70-85%; mild drought stress, 50-60%; moderate drought stress, 40-50%; and severe drought stress, 30-40%. Drought stress was imposed by limiting irrigation. Plants were sampled and physiological parameters w ere measured on 0, 2, 4, 6, 8, and 10 days after the commencement of droughts tress. The water potential of strawberry leaves decreased in the plants under mild, moderate, and severe stress during the course of the water stress treatment and exhibited a significant difference from the control. Strawberry leaves subjected to drought stress had higher accumulation of proline, sugars, and malondialdehyde, and higher activities of superoxide dismutase, peroxidase, and catalase than leaves of control plants. Malondialdehyde levels increased in parallel with the severity and duration of drought stress. By contrast, antioxidant enzyme activity displayed dynamic responses to drought stress, first increasing and subsequently decreasing as the severity and duration of drought stress increased. These results suggest that strawberry plants respond to drought stress by altering the activities of antioxidant enzymes and the levels of osmotically active metabolites. These biochemical response changes may confer adaptation to drought stress and improve the capacity of plants to withstand water-deficit conditions.

• 원예과학기술지
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• 제29권1호
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• pp.61-67
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• 2011

Barley (Hordeum vulgare L.) sprouts are a vegetable commonly used as a functional food material due to its high vitamin C concentration and antioxidant activity. In this experiment, we measured the changes in the antioxidant activity of several barley cultivars as well as in the concentrations of related compounds such as ascorbate and glutathione upon treatment with UV-B or salicylic acid (SA). The six barely cultivars were grown in a plant growth chamber (25/$18^{\circ}C$, 14/10 h, 200 ${\mu}mol{\cdot}m^{-1}{\cdot}s^{-1}$, 70% relative humidity) for 10 days. All barely cultivars showed different 1, 1-diphenyl-2-picryl-hydrazyl (DPPH) scavenging activities, which were increased by UV-B treatment and not by SA treatment. The changes in ascorbate concentrations were correlated with DPPH scavenging activity in both the treatments, suggesting that the antioxidant activity in barley sprouts was mainly dependent on ascorbate concentration. Furthermore, changes in ascorbate concentration showed similar tendencies to changes in free sugar concentration, especially glucose and sucrose, in both treatments. On the other hand, the concentrations of glutathione and cysteine highly increased by SA treatment, representing different tendencies compared to the DPPH scavenging activity and ascorbate concentration. 'Donghanchal' cultivar showed comparatively higher antioxidant activity, both constitutively and inducingly by UV-B treatment, with its higher concentrations of ascorbate and glutathione. These results suggest that barley sprouts could be used as a health-functional vegetable, contributing to the overall supply of antioxidant and sulfur-containing organic compounds.

• Nutrition Research and Practice
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• 제10권1호
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• pp.74-80
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• 2016

BACKGROUND/OBJECTIVES: Cardiovascular diseases is a major cause of death and is responsible for 23.8% of deaths in Korea. Clinical symptoms manifest in adulthood, but susceptibility begins in utero. Elevated homocysteine levels and adiposity might be linked to a greater risk in children as well as adults. We hypothesized that those who have simultaneous risk for folate and adiposity would be affected with elevated homocysteine levels at 3 years of age. SUBJECTS/METHODS: From the ongoing birth cohort at Ewha Womans University Mok-Dong Hospital, we compared adiposity parameters, serum homocysteine, and folate levels in 238 children (118 boys and 120 girls) at three years of age. The relationship between birth outcome, current weight and body mass index (BMI), postnatal growth, and homocysteine level were assessed using correlation and general linear model. Additionally, we assessed the combined effect between blood folate status and adiposity on current homocysteine levels. RESULTS: Birth characteristics were not correlated with homocysteine. Current weight, BMI, upper-arm circumference, skinfold thickness, waist circumference, and hip circumference were positively correlated with homocysteine at three years of age (P < 0.05). Folate level was negatively correlated with homocysteine at three years of age (P < 0.0001). A relative high anthropometric measure which is compatible with adiposity and low folate level was associated with high homocysteine levels. CONCLUSION: We found a combined effect of adiposity and folate levels with homocysteine levels at three years of age. This implicates the beneficial role of folate supplementation in the high-risk population at an early age.

• 농업생명과학연구
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• 제44권2호
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• pp.75-84
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• 2010

식품산업의 성장이 국내 농산물에 대한 원료 수요 확대로 직접적으로 이어지지 못하는 가장 큰 요인은 국산 농산물을 원료로 사용하기에는 높은 가격이나 안정적인 조달의 어려움과 같은 제약조건이 존재하기 때문이다. 식품산업과 국산 농산물 간의 연결고리가 이와 같이 약하다는 것은 사실상 국산 농산물을 식품으로 가공하여 전적으로 수요를 확대하는 데에는 한계가 존재하기 때문에 가능성 있는 품목을 발굴하는 것이 우선적인 과제라고 할 수 있다. 그간 정부에서는 농업과 식품산업 간의 연계를 강화하기 위해 다양한 정책을 실시하여왔다. 이 과정에서 지역단위의 농업조직이나 지역농업클러스터는 원료조달과 공급이라는 일차적인 연계를 넘어서 인프라 등을 공동으로 활용하는 방향으로 연계의 또 다른 방향을 모색할 수 있을 것이다. 지역농업과 식품기업의 연계에는 정부의 지역단위 농업활성화를 위한 각종 지원 정책 이외에도 국산 농산물을 원료로 사용하는 식품기업에 대한 인센티브를 부여하는 것도 효과적인 방안 중의 하나가 될 수 있을 것이다.

• 화훼연구
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• 제17권4호
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• pp.237-241
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• 2009

본 연구는 난과 식물의 아황산가스 피해증상을 정확하게 알기 위해 팔레놉시스, 심비디움, 온시디움을 이용하여 밀폐된 챔버에서 수행하였다. 가스처리는 아황산가스 농도를 0, 5, 10, 25, 50 ppm으로 하였고, 노출시간은 18시간, 온도 $25{\pm}5$, 습도 $50{\pm}5%$로 하였다. SPAD를 이용하여 가스처리 전후의 엽록소 함량을 측정하였으며, 피해엽율과 이온유출량을 조사하였다. 팔레놉시스의 경우 10ppm에서 피해엽율이 23.3%로 나타났고 심비디움, 온시디움의 경우 25ppm에서 각각 4.0, 4.4%의 피해가 나타나서 저항성이 강하다고 판단되었다. 주요증상은 초기에 잎의 뒷면이 수침상으로 나타났다가 급격히 잎이 황색으로 변하거나 탈색된다. 이온유출량은 아황산가스 농도가 높을수록 심하게 나타났고, 엽록소 함량은 아황산가스 농도가 높을수록 낮아졌다.

• 디지털융복합연구
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• 제17권10호
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• pp.103-113
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• 2019

본 논문은 한국에서의 석탄산업이 신기후체제의 출범 이후에도 여전히 중요하며, 일정한 물량의 석탄 비축을 통해 에너지원의 수급을 조절할 수 있는 능력을 지닐 필요성에 대한 근거를 제시하는데 있다. 최근 신재생에너지의 비중 증가는 석탄이나 석유와 같은 화석연료의 의존도를 낮출 것으로 전망된다. 그리고 이에 따라 석탄의 사용도 감소할 것으로 전망되지만, 아직 에너지원별 수요의 면에서나 전력 공급의 면에서 석탄이 에너지원으로서의 지위가 여전히 큰 것이 확인되었다. 석탄의 최적 비축 규모는 기존 연구의 연간 수요를 기준으로 추정하였다. 이러한 점에서 지속가능한 성장을 위한 산업적 정책으로서 석탄산업의 유지와 연간 최적 비축 규모의 결정은 매우 중요한 문제이다.

• 한국빅데이터학회지
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• 제6권1호
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• pp.1-12
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• 2021

모바일 기반 온라인 쇼핑의 급성장과 COVID-19로 인해 시작된 비대면 비즈니스의 성장은 택배와 같은 물류 서비스 수요를 폭발적인 증가를 이끌어냈다. 급격하게 성장한 수요에 대응하기 위해 대부분의 물류·유통기업들은 도심 내 풀필먼트 센터 구축을 통한 고객 서비스 수준 향상을 위해 노력하고 있다. 그러나, 높은 지가와 교통 체증 등과 같은 사회적 요인에 의해 도심 내 풀필먼트 센터를 확보하는 데 큰 어려움을 겪고 있다. 본 연구에서는 향후 고속도로에 스마트톨링 서비스가 전면 확대됨에 따라 유휴부지로 전환될 요금소 부지를 공유물류센터로 전환하기 위한 후보지 선정 방안을 제시한다. 이를 위해 사회연결망 분석을 통해 각 후보지들의 중심성을 분석하였으며, 중심성 평가의 특성에 따른 결과의 해석을 위한 네트워크 구조를 거리기반과 시간기반의 두 가지 방법으로 재설계하여 평가하였다. 누적된 중요도를 기준으로 적정 후보지 군을 선택하는데 활용될 수 있을 것이다.