• Molecules and Cells
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• 제37권7호
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• pp.547-553
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• 2014

Glioblastoma multiforme (GBM) is one of the most common brain malignancies and has a very poor prognosis. Recent evidence suggests that the presence of cancer stem cells (CSC) in GBM and the rare CSC subpopulation that is resistant to chemotherapy may be responsible for the treatment failure and unfavorable prognosis of GBM. A garlic-derived compound, Z-ajoene, has shown a range of biological activities, including anti-proliferative effects on several cancers. Here, we demonstrated for the first time that Z-ajoene specifically inhibits the growth of the GBM CSC population. CSC sphere-forming inhibition was achieved at a concentration that did not exhibit a cytotoxic effect in regular cell culture conditions. The specificity of this inhibitory effect on the CSC population was confirmed by detecting CSC cell surface marker CD133 expression and biochemical marker ALDH activity. In addition, stem cell-related mRNA profiling and real-time PCR revealed the differential expression of CSC-specific genes, including Notch, Wnt, and Hedgehog, upon treatment with Z-ajoene. A proteomic approach, i.e., reverse-phase protein array (RPPA) and Western blot analysis, showed decreased SMAD4, p-AKT, 14.3.3 and FOXO3A expression. The protein interaction map (http://string-db.org/) of the identified molecules suggested that the AKT, ERK/p38 and $TGF{\beta}$ signaling pathways are key mediators of Z-ajoene's action, which affects the transcriptional network that includes FOXO3A. These biological and bioinformatic analyses collectively demonstrate that Z-ajoene is a potential candidate for the treatment of GBM by specifically targeting GBM CSCs. We also show how this systemic approach strengthens the identification of new therapeutic agents that target CSCs.

• Asian Pacific Journal of Cancer Prevention
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• 제13권8호
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• pp.3841-3850
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• 2012

Objective: The objective of this study was to explore the knowledge and perceptions of Malaysian tradition healers towards cancer and cancer prevention. Methodology: A total of 25 participants agreed to participate in this qualitative study during the period from $20^{th}$ July 2011 until $24^{th}$ of September 2011. The proposal of this study was approved by the Ethics Committee of Management and Science University (MSU). Once the participant agreed to be interviewed, date, time and place of the interviews were determined. Consent form was obtained from participants before the interview began. Participants were briefed about the study and its purpose, and after asking their permissions, their replies were recorded. The data was organized into themes and analyzed manually. Results: Twenty-five Malaysian traditional healers participated in this qualitative study. The age of participants ranged between 26 to 78 years old. The majority were in the age group of 31-60 years old, male, Chinese, degree holders with a monthly income ranging from 1,000-5,000 Ringgit Malaysia (RM) and were married (56%, 80%, 48%, 52%, 68%, 84% respectively). The majority defined cancer as having high cholesterol or abscess accumulation. A few of them defined cancer as a type of cell growth. The majority mentioned that food and unhealthy lifestyles are the primary causes of cancer. Surprisingly some of them mentioned that cancer is caused by interference by ghosts. Regarding the diagnosis of cancer, the majority mentioned that they refer their patients to modern physicians' medical report when it comes to diagnosing or treating patients with cancer. The most common cancers that many patients came to seek treatment were breast cancers, followed by colon cancers, liver and lung cancers. Conclusions: Despite good knowledgeabout the causes of cancer among traditional healers, misconceptions still exist. Insufficient knowledge about the definition of cancer was noted among the traditional healers. This urges immediate action by the Ministry of Health of Malaysia to set up a strict regulation and regular monitoring of the traditional healers nationally. Traditional and Complementary Medicine may be integrated into the healthcare system and need to have sustained cooperation for the benefit of patients since about 80% of patients use traditional medicines.

• 한국철도학회:학술대회논문집
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• 한국철도학회 2010년도 춘계학술대회 논문집
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• pp.1603-1614
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• 2010

교토의정서 및 발리로드맵 이후 우리나라도 2013년부터 온실가스배출감축대상국에 포함될 예정임에 따라 친환경물류에 대한 정부차원의 대책마련도 시급한 상황이다. 본 연구는 이러한 정부차원의 친환경 녹색성장 정책에 맞춰 가장 친환경적인 철도물류 및 복합운송에 대한 활성화를 본격 연구하게 되었다. 본 연구에서 나타난 의왕ICD 터미널 내 철도물류시설의 주요 문제점으로는 하치장 부족, 공용 CY 비활성화, 비주주사 이용불편 등으로 나타나고 있으며, 철도물류시설의 주요 개선방안으로는 하치장의 추가 확장, 공용CY 활성화를 위한 철도공사 직영 및 상시하역체계 구축 조성 등의 철도물류시설개선이 필요한 것으로 나타났다. 이러한 철도물류시설의 개선은 철도공사 또는 업체의 노력으로만 해결될 수 있는 사안은 아니다. 철도물류시설과 복합화물 터미널은 대규모의 투자자금이 필요한 시설이며 사회간접자본(SOC)성격이 강하므로 민간차원에서 개선하는 데에는 한계가 있으므로 정부차원에서 보다 적극적인 개선방안을 모색할 필요가 있다. 이러한 정부차원의 철도물류시설 개선은 친환경녹색물류에 대한 지원정책으로 물류정책기본법에 의해 지원이 가능하므로 세부적인 지침 등을 마련하여 복합화물터미널이 근본적으로 활성화 될 수 있는 계기가 마련되어야 할 것이다.

• 한국식품위생안전성학회지
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• 제13권4호
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• pp.406-411
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• 1998

조리방법이 시판 햄 및 소시지의 NA 생성에 미치는 영향을 조사하기 위해 가스레인지 (GR), 전자레인지 (ER) 및 데친 후 전자레인지(BE)로 조리하였을 때 생성되는 NA를 GC-TEA로 분석하였고, 동시에 NA 생성에 영향을 미칠 것으로 판단된 질산염 및 아질산염도 분석하였다. 조리 전 햄의 질산염 및 아질산염질소의 함량은 각각 2.0, 1.8mg/kg이었으나 조리과정 중 단일 햄에서는 미량 증가하였고, 혼합햄에서 질산염질소는 감소하였으나 아질산 염질소는 증가하였다. 소시지의 질산염 및 아질산염 질소는 각각 1.8, 0.9mg/kg이었으며 조리과정 중 질산염질소는 감소한 반면 아질산염질소는 미량 증가하였다. 햄과 소시지 모두에서 NA는 NDMA만이 검출되었는데 조리방법에 관계없이 모두 조리과정 중 증가하는 경향을 보였고 가스레인지로 조리한 경우는 평균 6.0~70.7배, 전자레인지 및 데친후 전자레인지로 조리한 경우는 각각 평균 2.4~39~2, 7.0~56.3배 증가하였다.

• 한국학교수학회논문집
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• 제17권3호
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• pp.385-408
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• 2014

본 연구는 초등학교 방과후학교 수학과 수업을 담당하고 있는 강사들을 대상으로 그 운영실태를 조사했다는 점에서 의의를 갖는다. 이를 위해 부산광역시 초등학교 100개교에서 방과후학교 수학 프로그램을 담당하고 있는 강사 115명을 대상으로 설문조사를 실시하였다. 설문의 내용은 강사 관련 실태, 학생 관련 실태, 수업 관련 실태로 구분되어 있으며, 이 가운데 특히 수업 관련 실태의 경우 수업의 운영과 관련된 전반적인 목표, 시간과 횟수, 교재와 내용, 평가에 이르기까지 강사들의 답변을 종합하였다. 또한 서술형 설문 문항을 통해서 방과후학교 프로그램 진행에서의 어려움과 함께 개선이 요구되는 부분을 정리하였다. 초등학교 수학과 방과후학교 프로그램의 경우 그 유형은 교과 중심의 예습 복습에 초점을 맞추는 교과수학, 교과와 함께 창의적 활동과 체험을 고려한 창의수학, 그리고 연산 중심의 주산/암산으로 구분할 수 있었다. 강사들의 공통 의견으로는 강사의 전문성 확보를 위한 연수 프로그램의 개발을 비롯하여 수학과 방과후학교에 적합한 프로그램과 교재의 개발, 그리고 다양한 형태의 수학 수업을 진행하기 위한 강사의 노력과 함께 학부모의 수학과 방과후학교 프로그램에 대한 인식의 변화, 학교의 방과후학교 지원 등을 꼽았다. 이러한 실태조사를 통해 초등학교에서 방과후학교 수학과 프로그램이 방과후학교의 목적에 부합할 수 있는 개선 방안이 마련되어야 할 것이다.

• 한국해안·해양공학회논문집
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• 제24권3호
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• pp.189-202
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• 2012

공기실 내에서 해수면의 상하운동을 공기흐름으로 변환하고, 이를 터빈의 구동력으로 이용하는 진동수주형(OWC; Oscillating Water Column)의 파력발전시스템은 파랑에너지흡수장치 중에 가장 효율적인 것으로 알려져 있다. 본 연구에서는 3차원불규칙파수치파동수로에 기초한 3D-NIT(3-Dimensional Numerical Irregular wave Tank)모델을 규칙파동장에 적용하여 산정된 공기실 내 수위변동의 시간변화로부터 공기흐름속도를 추정하고, 이의 결과와 수리모형실험으로부터 얻어진 공기흐름속도와를 비교하여 본 3D-NIT모델의 적용성을 검토하였다. 또한, 불규칙파동장하의 OWC파력발전구조물에서 공기실 내 공기흐름의 해석에 3D-NIT모델을 적용하여 입사주파수스펙트럼의 변화에 따른 공기흐름 주파수스펙트럼의 변화특성, 구조물의 존재여부에 따른 공기실 위치에서 주파수스펙트럼의 변화특성 및 구조물에 의한 반사율의 변화특성 등을 검토하였다. 이로부터 공기실 내에서 수위변동 및 공기흐름의 시계열 자료에서 위상차가 존재하며, 공기실 내의 공진에 의해 수위변동의 주파수스펙트럼에서 첨두치가 증폭되는 현상 등을 알 수 있었다.

• Journal of Preventive Medicine and Public Health
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• 제26권1호
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• pp.20-36
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• 1993

Work-related low back pain is one of the major factors that cause the loss of working power especially in actively working age, therefore controlling the work-related low back pain is one of the major issues in the field of industrial safety and health. This study was performed for detecting the risk factors and proposing the efficient control program of the work-related low back pain. The subjects were male workers employed at the manufacturing industry in Pusan with longer than 6 months' working duration. The data was collected by self-reported questionnaire and interview from May 1 to October 31,1992. The contents of questionnaire were as follows:. the experience of low back pain within the recent one month, general characteristics(age, marital status, education level, religion, regular exercise), physical characteristics(height, weight), employment status(working duration, daily working hours, rest during work, satis(action fur the job), type of work and working environments(posture, forward bending, lifting and movement, satisfaction for table and chair). The result was analyzed for 608 entire respondents by case-control comparative method. The number of cases was 152 with a history of work-related low back pain, so the relative frequency of self-reported work-related low back pain was 25.0%, and the number of controls was 344 without any history of low back pain. As a result, two characteristics of the employment status(working duration, satisfaction fur the job) and all characteristics of type of work and working environments showed a statistical significance between the case and control groups(p<0.01). The Oddb ratios of these variables for Work-related low back pain were calculated. They were 7.88 for the satisfaction fur chair, 7.86 for lifting and movement,3.31 for satisfaction for table, and 2.22 fur bending forward(p<0.01). And for the multivariate logistic regression analysis confirmed that unsatisfaction for table was independent risk factor for Work-related low back pain. In summary, though this study was based upon the self-reported questionnaire and the subjective complaints, the satisfaction for chair, lifting and movement, satisfaction for table, and bending forward concerned with the type of work and working environments were considered as the main factors causing the work-related low back pain, and the work-related low back pain may be preventable by the ergonomic control of these factors.

• 지질공학
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• 제26권2호
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• pp.207-221
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• 2016

최근 국내 필댐 및 저수지의 노후화가 급속도로 진행되고 있으며, 이에 따른 제체의 손상이나 붕괴사고가 잇따르고 있다. 그러나 필댐의 핵심적인 차수기능을 담당하는 코어층에 대한 열화와 잠재 위해성 분류에 대한 연구는 거의 미진한 실정이다. 본 연구에서는 노후화가 진행 중인 13개 기존 코어형 필댐에 대해(준공 후 경과년수 9-52년, 댐 높이 22-67 m), 무수보링에 의한 코어층 시추 지반조사와 표준관입시험, 2D 및 3D 전기비저항탐사, 물성·역학시험을 수행하여 공학적 기반의 잠재적 위해성 유형을 국내 최초로 분류, 제시하였다. 중심코어층에 대한 무수보링 시추 지반조사 결과, 코어재 잠재 위해성 유형을 국부적 포화대, 신속한 공내수 유입, 조립질 혼재 코어재, 상대적 저 강성의 네 종류로 분류하였다. 코어형 필댐의 잠재 위해성 유형 중국부적 포화대 유형은 코어존 내 유로의 성장 및 발달에 의한 내적침식의 가능성이 존재하므로 가장 위험한 위해성으로 분류 되었으며, 코어재의 열화 등급에 따라 적절한 차수능 회복을 위한 보수보강이 필요한 것으로 판단되었다. 그 외 세 가지 위해성 유형은 즉각적인 조치보다는 지속적인 모니터링과 정기 검사의 강화가 필요할 것으로 검토되었다. 본 연구대상 댐의 분석 결과, 준공 후 경과년수와 댐 코어재의 열화 양상과는 상관성이 크게 없는 것으로 나타났다. 본 연구 결과는 코어형 필댐의 노후화에 따른 댐의 안전관리와 성능개선에 있어 유용한 자료로 활용 가능할 것으로 판단된다.

• 한국병원경영학회지
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• 제14권1호
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• pp.123-144
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• 2009

Recently, a long strike by hospital labor union emerged as a serious social issue. During the Worldcup Games in June, 2002, labor strikes broke up at 'C', 'K' and other hospitals, and in 2007, 'Y' hospital suffered much from a strike. Such series of extreme labor disputes have awakened people of importance of a more stable labor-management relationship for the medical institutions responsible for people's health than any other business organization. The purpose of this study was to examine the labor-management disputes at 'Y' hospital in 2007 and 'C' and 'K' hospitals in 2002. The results of this study can be summarized as follows; First, requests of the labor union such as pay raise, reemployment of the irregular workers as regular employees and participation of the labor union in personnel affairs are the long-held or core issues suffered by the medical institutions. Such issues are not independent from each other but complicated with each other surrounding the pay raise. Accordingly, it is not easy to determine the genuine bone of issue for labor-management disputes. Second, the model type of disputes between labor and management at medical institutions may be strike. However, it is conceived that the type of disputes would be subject to change as the essential medical service area system began to be operated since 2008. Third, the common characteristic of the labor strike among the 3 sample hospitals was occupation of the hospital lobby for a sit-in strike to maximize the negative effects of strike. Article 42 (Prohibition of Violence) of Labor Union and Labor Relation Coordination Act prohibits occupation of production or other important business facilities. In addition, since Ministry of Labor interprets that the hospital lobby belongs to the important business facilities enumerated by Article 42 of the above act, occupation of the hospital lobby for a sit-in strike may be too controversial to be admitted as a fair act of labor dispute when its legitimacy should be judged. Fourth, the counter-measures taken by the hospitals against the strike were observance of the principle 'no labor no pay,' closure, legal action, accusation, claim for recovery of damage, provisional seizure, disciplinary punishment, etc., but the principle of 'no labor, no pay' was not applied in a fair manner by 'C' and 'K' hospitals. However, 'Y' hospital applied this principle thoroughly to the strike; the hospital conduced to correction of the wrong labor-management relationship by refusing inclusion in the labor collective agreement of a provision about payment of wage during the period of strike or labor union's request to that effect during a strike. In addition, 'Y' hospital took an effective measure to end the strike earlier by notifying the labor union of cancellation of the collective agreement and banning the unionists from entering the hospital.

• Clinical and Experimental Reproductive Medicine
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• 제26권3호
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• pp.483-490
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• 1999

Objectives: Polycystic ovary syndrome (PCOS) has the feature of excessive LH, hyperandrogenism and disturbance of folliculogenesis. Also, insulin, IGF-I and IGFBP-l are involved in the pathogenesis of PCOS. Various surgical and medical therapies have been used and the action mechanisms are related to the endocrine effect. Laparoscopic ovarian electrocautery or laser vaporization is effective in the restoration of ovulation and normal menstrual cycle with minimal invasive procedure especially in the patients resistant to medical therapy. Clomiphen citrate (CC) is used for the ovulation induction in pcas and the resistance is known to be related to insulin, IGF-I, IGFBP-l levels. This study was performed to evaluate the effect of the laparoscopic laser vaporization on the levels of LH, FSH, testosterone, IGF-I and IGFBP-l and on the ovarian response to clomiphen citrate in patients with CC-resistant PCOS. Materials and Methods: The fasting basal serum LH, FSH, testosterone, IGF-I and IGFBP-l level were measured in 10 PCOS patients with CC-resistance and 7 normal controls with regular menstrual cycle. In PCOS, after laparoscopic $CO_2$ laser vaporization, endocrine levels were measured in 1 week interval for 4 weeks and then compared with preoperative levels. Results: In PCOS group, mean serum LH/FSH ratio, testosterone, IGF-I levels were higher and IGFBP-l level was lower than control. LH/FSH ratio decreased from $2.51{\pm}0.67$ to $1.7{\pm}0.6$ (p<0.05) in 2 weeks, to $0.56{\pm}0.2$ (p<0.01) in 3 weeks and to $1.41{\pm}0.3$ (p<0.01) in 4 weeks after operation. Testosterone level decreased from $1.51{\pm}0.82ng/ml$ to $0.65{\pm}0.34ng/ml$ (p<0.05) in 2 weeks, to $0.56{\pm}0.67ng/ml $(p<0.01) in 3 weeks after operation. IGF-I level also decreased from $436{\pm}47.5{\mu}g/l$ to $187{\pm}38{\mu}g/l$ (p<0.0l) in 1 week, to $167{\pm}42{\mu}g/l$ (p<0.01) in 2 weeks, $179{\pm}55{\mu}g/l$ (p<0.01) in 3 weeks and to $120{\pm}43{\mu}g/l$ (p<0.01) in 4 weeks after operation. IGFBP-l level showed no significant change. In 8 of 10 PCOS patients, ovulation was induced with low dose clomiphen citrate. Conclusion: Laparoscopic $CO_2$ laser vaporization restores normal menstrual cycle and ovulation through endocrine effect of decreasing LH/FSH ratio, testosterone and IGF-I level and increases the response to CC. Therefore it is useful for restoration of normal menstruation and induction of ovulation in CC resistant PCOS patients.