• 한국정밀공학회지
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• 제26권11호
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• pp.108-115
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• 2009

In metal forming technologies, the stamping process is one of the significant manufacturing processes to produce sheet metal components. It is important to design stamping process which can produce sound products without defect such as fracture and wrinkle. The objective of this study is to propose the feasible formability diagram which denotes the safe region without fracture and wrinkle for effective design of stamping process. To determine the feasible formability diagram, FE-analyses were firstly performed for the combinations of process parameters and then the characteristic values for fracture and wrinkle were estimated from the results of FE-analyses based on forming limit diagram. The characteristic values were extended through training of the artificial neural network. The feasible formability diagram was finally determined for various combinations of process parameters. The stamping process of turret suspension to support suspension module was taken as an example to verify the effectiveness of feasible formability diagram. The results of FE-analyses for process conditions within fracture and wrinkle as well as safe regions were in good agreement with experimental ones.

• 환경생물
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• 제31권3호
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• pp.225-231
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• 2013

동해안의 암반조간대 무척추동물상의 분포 특성을 연구하기 위하여 독도를 비롯한 울릉도, 경상북도의 경주, 포항, 영덕, 울진 그리고 강원도 등 총 19개 정점의 암반 조간대무척추동물상을 조사하여 출현종의 유사도를 공통종의 출현비율 (%)과 Bray-Curtis similarity matrix를 이용한 집괴분석과 MDS를 이용하여 분석하였으며 SIMPER를 이용하여 독도와 그 외의 동해안 암반저서 무척추동물의 특징종을 선출하였다. 공통종의 출현비와 집괴분석결과, 가장 가까이 위치한 울릉도를 제외하면, 경상북도의 영덕이 가장 높은 유사도를 보였으나, 전체적으로 독도 암반저서생태계는 다른 동해안 연안과 차이를 보인 것으로 나타났다.

• 식물병연구
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• 제10권2호
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• pp.154-158
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• 2004

오이에 발생하는 CGMMV 발병과 품종별 종자감염과의 관계를 분석하기 위하여 1999년부터 2002년까지 전남지역의 오이 주요 재배지에서 CGMMV가 발생한 포장의 품종과 발생정도를 조사하고 시판되는 종자를 수집하여 종자감염여부와 CGMMV에 대한 품종저항성 검정을 실시하였다. 조사 연도와 품종에 따라 발생정도의 차이는 있었으나 장형낙합은 4년 동안 발생하였고, 장죽청장은 3년, 겨우살이와 겨울나기는 2년 동안 발생되었다. 이들 품종에 대한 종자의 ELISA검정을 실시한 결과 장형낙합과 장죽청장에서 CGMMV가 검출되었으며, 장죽청장에서는 생물검정에서 양성을 나타냈다. 또한 국내에서 재배되고 있는 34품종에 대한 CGMMV의 저항성을 검정한 결과 폿트시험에서는 모두 모자이크 병징을 보여 차이가 없었으나 포장시험에서는 한복삼척에서만 마일드모자이크 병징을 보였다.

• 대한바이러스학회지
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• 제28권2호
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• pp.129-138
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• 1998

Bovine growth hormone (bGH) gene was expressed in an insect Spodoptera frugiperda cell line using a Baculovirus, Hyphantria cunea nuclear polyhedrosis virus (HcNPV). The bGH gene in pbGH plasmid was sequenced and amplified by PCR technique with two primers containing NcoI sites. The bGH gene consisted of 654 bp (217 amino acid residues), the 5'-untranslated region of the cloned bGH cDNA contains 56 bp, and the 3'-untranslated region contains 145 bp and two pallindromic regions. The amplified bGH gene DNA fragment (654 bp) was inserted into the NcoI site of the pHcEVII vector, which was named pHcbGH. The pHcbGH transfer vector DNA and the wild type HcNPV DNA were cotransfected into S. frugiperda cells to construct a recombinant virus. Eight recombinant viruses were selected and named HcbGH. One clone, HcbGH-4-1 showed largest plaque size, therefore the recombinant virus was further studied. The multiplication pattern of the recombinant HcbGH-4-1 was similar to that of the wild type HcNPV. The bGH gene DNA in the HcbGH-4-1 recombinant was confirmed by Southern blot hybridization. The amount of the bGH (217 amino acid residues, 21 kDa) produced in S. frugiperda cells infected with the HcbGH-4-1 recombinant was approximately 5.5 ng per ml ($10^6$ cells) by radioimmunoassay.

• 한국지하수토양환경학회지:지하수토양환경
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• 제23권1호
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• pp.92-105
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• 2018

In this study, the projected land use area in 2030 for major crop production was estimated in Jeju Island using land cover map, and corresponding agricultural water demand for 40 sub-regions was quantitatively assessed using the future climate change scenario (RCP 4.5). Estimated basic unit of water demand in 2030 was the highest in the western region, and the lowest in the eastern region. Monthly maximum agricultural water demand analysis revealed that water demand in August of 2030 substantially increased, suggesting the climate of Jeju Island is changing to a subtropical climate in 2030. Agricultural water demand for sub-region in 2030 was calculated by multiplying the target area of the water supply excluding the area not in use in winter season by the basic unit of water demand, and the maximum and minimum values were estimated to be $306,626m^3/day$ at Seogwipo downtown region and $77,967m^3/day$ at Hallim region, respectively. Consequently, total agricultural water demand in Jeju Island in 2030 was estimated to be $1,848,010m^3/day$.

• 한국약용작물학회지
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• 제22권6호
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• pp.489-496
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• 2014

Polygonatum is a genus placed in the family Liliaceae, distributed throughout the Northern Hemisphere and 16 of the species are grown naturally in Korea. In oriental medicine, the rhizomes of Polygonatum have been used as two different medicines, Okjuk (Polygonati odorati Rhizoma) and Hwangjeong (Polygonati Rhizoma). However, it is difficult to identify the morphological and chemical differences between the medicinal groups and thus easy to confuse the one with the other. Therefore, a clear classification standard needs to be established so as to be able to discriminate between them. In the study, the morphological characteristics of the plants, Polygonatum spp., were examined. Then, the differences in SNPs among the DNA sequences of 7 of the Polygonatum spp. and 1 of the Disporum spp. were analyzed by DNA barcoding with rpoC1, rpoB2, matK, and psbA-trnH of the cpDNA region. In the results, three regions, rpoC1, rpoB2, and matK were useful for discriminating the species, P. stenophyllum and P. sibiricum. Furthermore, it was possible to discriminate the individual germplasm within the species by using the combination of the results obtained from rpoB2, rpoC1, and matK.

• The Korean Journal of Physiology and Pharmacology
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• 제7권3호
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• pp.157-162
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• 2003

Our earlier studies found a significant correlation between the activities of ranitidine N-oxidation catalyzed by hepatic flavin-containing monooxygenase (FMO) and the presence of mutations in exon 4 (E158K) and exon 7 (E308G) of the FMO3 gene in Korean volunteers. However, caffeine N-1 demethylation (which is also partially catalyzed by FMO) was not significantly correlated with these FMO3 mutations. In this study, we examined another common mutation (V257M) in exon 6 of FMO3 gene. The V257M variant, which is caused by a point mutation (G769A), was commonly observed (13.21% allele frequency) in our subjects (n=159). This point mutation causes a substitution of $Val^{257}$ to $Met^{257}$, with transformation of the secondary structure. The presence of this mutant allele correlated significantly with a reduction in caffeine N-1-demethylating activity, but was not correlated with the activity of N-oxidation of ranitidine. In a family study, the low FMO activity observed in a person heterozygous for a nonsense mutation in exon 4 (G148X) and heterozygous for missense mutation in exon 6 (V257M) of FMO3 was attributed to the mutations. Our results suggest that various point mutations in the coding regions of FMO3 may influence FMO3 activity according to the probe substrates of varying chemical structure that correlate with each mutation on the FMO3 gene.

• Parasites, Hosts and Diseases
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• 제50권3호
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• pp.225-227
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• 2012

The seroepidemiological status of toxoplasmosis was surveyed among the residents of Cheorwon-gun, Gangwon-do by means of ELISA using a crude extract antigen of Toxoplasma gondii. The sera of 1,661 adult residents (866 males and 795 females) were collected and checked for IgG antibody titers, which showed 17.0% positive rate (282 sera). The positive rate was significantly different between the sex; 20.6% for males and 13.1% for females (P<0.05). The positive rates were higher in fifties of males (28.7%) and forties of females (20.0%). This positive rate of toxoplasmosis in Cheorwon-gun residents is regarded as the highest among the surveys of different geographical regions of Korea. This high positive rate may due in part to peculiar geographical locality of the surveyed area near the naturally well preserved demilitarized zone (DMZ) or presumably consumption of the pork imported from high endemic nations. Therefore, it is necessary to study further the epidemiology of toxoplasmosis in Cheorwon-gun.

• Parasites, Hosts and Diseases
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• 제53권3호
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• pp.271-277
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• 2015

The genetic diversity of Schistosoma haematobium remains largely unstudied in comparison to that of Schistosoma mansoni. To characterize the extent of genetic diversity in S. haematobium among its definitive host (humans), we collected S. haematobium eggs from the urine of 73 infected schoolchildren at 5 primary schools in White Nile State, Sudan, and then performed a randomly amplified polymorphic DNA marker ITS2 by PCR-RFLP analysis. Among 73 S. haematobium egg-positive cases, 13 were selected based on the presence of the S. haematobium satellite markers A4 and B2 in their genomic DNA, and used for RFLP analysis. The 13 samples were subjected to an RFLP analysis of the S. haematobium ITS2 region; however, there was no variation in size among the fragments. Compared to the ITS2 sequences obtained for S. haematobium from Kenya, the nucleotide sequences of the ITS2 regions of S. haematobium from 4 areas in Sudan were consistent with those from Kenya (> 99%). In this study, we demonstrate for the first time that most of the S. haematobium population in Sudan consists of a pan-African S. haematobium genotype; however, we also report the discovery of Kenyan strain inflow into White Nile, Sudan.

• Molecules and Cells
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• 제21권3호
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• pp.360-366
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• 2006

Up to 35% of the rice genome consists of various kinds of transposons, and CACTA and MITE are two of the major class 2 DNA transposons in the genome. We have employed the consensus sequences of Rim2/Hipa CACTA, Stowaway MITE Pangrangja, and Tourist MITE Ditto for transposon display (TD) analysis to locate them on a genetic map, with 58 SSR markers used to anchor them. The TD analysis produced a high profile of the polymorphisms between the parental lines, Oryza sativa var. Gihobyeo/O. sativa var. Milyang, in intraspecific $F_{15}$ RIL lines, locating 368 markers of Rim2/Hipa CACTA, 78 markers of Tourist MITE Ditto, and 22 markers of Stowaway MITE Pangrangja. In the segregation analysis, non-parental segregating bands and segregation distortion bands were observed. The recombinant genetic map spans 3023.9 cM, with 5.7 cM the average distance between markers. The TD markers were distributed unequally on the chromosomes because many TD markers were located in pericentric chromosomal regions except in the cases of chromosomes 2, 3, 6 and 9. Although the number of transposon markers was not sufficient to include all rice class 2 transposons, the current map of CACTA and MITE transposons should provide new insight into the genome organization of rice since no previous DNA transposon map is available.