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http://dx.doi.org/10.7783/KJMCS.2014.22.6.489

Morphological Characteristics and Phylogenetic Analysis of Polygonatum Species Based on Chloroplast DNA Sequences  

Kim, Jeong Hun (Department of Biological & Environmental Science, Dongguk University)
Seo, Jae Wan (Department of Biological & Environmental Science, Dongguk University)
Byeon, Ji Hui (Department of Biological & Environmental Science, Dongguk University)
Ahn, Young Sup (Department of Herb Crop Resources, NIHHS, RDA)
Cha, Seon Woo (Department of Herb Crop Resources, NIHHS, RDA)
Cho, Joon Hyeong (Department of Biological & Environmental Science, Dongguk University)
Publication Information
Korean Journal of Medicinal Crop Science / v.22, no.6, 2014 , pp. 489-496 More about this Journal
Abstract
Polygonatum is a genus placed in the family Liliaceae, distributed throughout the Northern Hemisphere and 16 of the species are grown naturally in Korea. In oriental medicine, the rhizomes of Polygonatum have been used as two different medicines, Okjuk (Polygonati odorati Rhizoma) and Hwangjeong (Polygonati Rhizoma). However, it is difficult to identify the morphological and chemical differences between the medicinal groups and thus easy to confuse the one with the other. Therefore, a clear classification standard needs to be established so as to be able to discriminate between them. In the study, the morphological characteristics of the plants, Polygonatum spp., were examined. Then, the differences in SNPs among the DNA sequences of 7 of the Polygonatum spp. and 1 of the Disporum spp. were analyzed by DNA barcoding with rpoC1, rpoB2, matK, and psbA-trnH of the cpDNA region. In the results, three regions, rpoC1, rpoB2, and matK were useful for discriminating the species, P. stenophyllum and P. sibiricum. Furthermore, it was possible to discriminate the individual germplasm within the species by using the combination of the results obtained from rpoB2, rpoC1, and matK.
Keywords
DNA Barcoding; Polygonatum; SNPs; rpoC1; rpoB2; matK; psbA-trnH;
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Times Cited By KSCI : 7  (Citation Analysis)
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