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Cytokine expression and localization during the development of glomerulosclerosis in FGS mice

  • Park, Sang-Joon;Lee, Sae-Bom;Lee, Young-Ho;Ryu, Si-Yun;Jeong, Kyu-Shik;Lee, Cha-Soo
    • Korean Journal of Veterinary Pathology
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    • v.3 no.1
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    • pp.15-25
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    • 1999
  • To elucidate the mechanism of age-related development in FGS/NgaKIST mice with spontaneous glomerulosclerotic lesion, we examined expression and localization of various cytokine mRNA in the kidney in the progression of diseases. This mouse model is the first to develop spontanously occuring glomerosclerotic lesion in the kidney. In this study, we detected the up-regulation of local cytokine genes such as IL-1$\beta$, IL-2, IL-6, IL-10, TNF-$\alpha$, TGF-$\beta$, and IFN- $\gamma$ in the kidneys. In RT-PCR and Southern blot analysis, we detected gradual expressions of cytokine mRNA of IL-1$\beta$, IL-2, IL-6, IFN- $\gamma$, and TNF $\alpha$ mRNA during the course of disease. Other cytokines including IL -10 and TGF -$\beta$ were found to be appeared the slightly expressed level at 3 to 12 weeks before onset of inflammatory lesion but they are highly expressed at the end-stage of the disease accompaning high proteinurea and wasting. In situ RT-PCR, each cytokine mRNA were specifically localized in a variety of cells including mesangial, endothelial, parietal epithelial, tubular epithelial, arterial muscle cell, and infiltrated inflammatory cells. In addition, TNF - $\alpha$was detected moderately in the visceral and parietal epithelial cell, but weakly in endothelial and mesangial cells, whereas IL-1 $\beta$ and IL -6 were strong in mesangial regions. IL-6 and TNF- $\alpha$ was highly localized in the damaged proximal and collecting tubules. Especially, TGF -$\beta$ mRNA was highly found in mesangial cells within glomerulus and interstitium during the end-stage of this disease.. These results indicate that pro inflammatory cytokines such as IL-1 $\beta$, IL-2, IL-6, and TNF- $\alpha$ were gradually expressed from the early stage of this disease to the end-stage, and that IL-10 and TGF-$\beta$ may be important in the accumulation of extracellular matrix(ECM) within glomerulus and periglomerular fibrosis in the progression of this disease as well as tissue destruction in end-stage of this disease.

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A Study of Market Segmentation of Optical Shop Based on Customer's Values (고객의 가치관에 따른 안경원의 시장세분화에 관한 연구)

  • Lee, Jung-Kyu;Cha, Jung-Won
    • Journal of Korean Ophthalmic Optics Society
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    • v.20 no.4
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    • pp.405-414
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    • 2015
  • Purpose: We analyse characteristics of optical shop customer's segmented market by using clustering analysis, and we expect it would be a useful indicator of marketing strategy for optical shops. Methods: Survey was conducted from March 10 to March 31, 2015. The survey asked customers who have visited optical shops in Seoul and Northern Gyeonggi-do regions, and analyzed by utilizing SPSS v.10.0 statistical package program. The analysing methods are frequency analysis, factor analysis about variable of values, clustering analysis for market segmentation, and crosstabs. Results: The market is segmented based on values. In the process of establishing marketing strategy, it is useful to establish strategy by classifying customers into 3 types of cluster; "middle level value oriented cluster", "high level value oriented cluster", "high level value oriented and non-religious cluster". In marketing strategy of progressive lenses, it turned out that the most important strategy is to target self-employed person in "middle level value oriented cluster". Conclusions: As a result of market segmentation by using clustering analysis, it was classified into 3 types of cluster, and we found that most important customer for progressive lenses is self-employed person in "middle level value oriented cluster" who is more than 41 years old.

A Survey on the Sensory Preference for Making Summer Kimchi by Nationwide Region (여름철 배추김치 담금시 지역별 관능적 선호도 조사)

  • Cha, Yong-Jun;Kim, Hun;Cho, Wo-Jin;Jung, Yeon-Jung;Lee, Young-Mi;Kim, Eun-Jeong
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.32 no.3
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    • pp.393-399
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    • 2003
  • The purpose of this study was to investigate the preferred methods for making summer kimchi as a basic research of making commercial kimchi. Questionnaire were collected from 590 housewives nationwide in Korea, and the data were analyzed by SPSS program. The results were as follows: (1) The average salting time of Chinese cabbage was 3~5 hrs when the combined method of dry and brine salting was used, regardless of the region. (2) Seven jeotkals (salt-fermented fishes) including anchovy, anchovy juice, shrimp, northern sand lance juice, hair-tail viscera, flatfish and yellow corvenia were mainly used in kimchi. Among them, anchovy and anchovy juice jeotkals were preferred to all others in Southern area (Busan, Gwangju, Gyeongnam, Gyeongbuk, Jeonnam), while shrimp jeotkal in Seoul, Gwangju, Jeonbuk, Chungnam and Jeju, and northern sand lance juice jeotkal in Daegu, Daejeon and Gyeonggi, respectively. In most regions, however, blending type of 2 jeotkals was used in kimchi. (3) Eleven ingredients such as red pepper, garlic, ginger, green onion, radish, leek, onion, carrot, sugar, sesame and MSG were used as basic components for making kimchi. In particular, MSG was used as a basic ingredient regardless of region and age. However, a standard taste for making kimchi was depended on housewife in this study.

Studies on Analyzing Meteorological Elements Related with Yield of Tricholoma matsutake (S. Ito et Imai) Singer. (송이(Tricholoma matsutake)생산(生産)과 관련되는 기후특성분석(氣候特性分析))

  • Kang, An-Seok;Cha, Dong-Yeol;Kim, Yang-Sup;Park, Yong-Hwan;You, Chang-Hyun
    • The Korean Journal of Mycology
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    • v.17 no.2
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    • pp.51-56
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    • 1989
  • This study was carried out to obtain basic information elucidating relationship meteorological elements and yield of Tricholoma matsutake. The yields of pine mushroom in seven areas including Yangyang-gun more than 30,000 kg. Pine mushrooms were generally distributed in the red pine (Pinus densiflora) forests of East-Coast Region, Sobaek and Taebaek-Mountainous Regions, Yeongnam-Central Region and North-Central Region of Korea. Yields of pine mushroom were significant correlation with precipitation during April-October in last years, average minima temperature during July-September, rainy days during Aug. 21-Sep. 10 and precipitation during Sep. 11-30, etc. Air and soil temperature during pine mushroom yield period were 15-25 and $15-20^{\circ}C$, respectively. The first collection date of pine mushroom could be predicted by measuring the soil temperature at 5 cm depth.

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Characterization of the genomes of Aujeszky's disease virus isolated in Korea (국내분리 오제스키병 바이러스의 게놈 유전자 특성 분석)

  • Hyun, Bang-Hun;Kim, In-Joong;Pyo, Hyun-Mi;Cha, Sang-Ho;Park, Ji-Yeun;Song, Jae-Young;Cho, In-Soo;Yang, Chang-Bum;An, Soo-Hwan;Lee, Joong-Bok
    • Korean Journal of Veterinary Research
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    • v.49 no.1
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    • pp.45-57
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    • 2009
  • The molecular genetic characterization of Aujeszky's disease virus (ADV) Yangsan strain (ADVYS), a Korean isolate, was investigated by analyzing the electrophoresis patterns and the physical maps of the viral DNA digested with various endonucleases. To establish DNA library for ADV-YS, twelve major BamHI restricted segments were cloned. Each location of the segments in the ADV genome was determined by sequence comparison with the sequences reported in Genbank and those sequences of the both termini of the segments. Physical maps were constructed based on the electrophoresis patterns of the digested viral DNA by restriction endonuclease and the results of Southern blot analyses with various DIG labeled probes originated from those of enzyme restricted segments of virulent (Shope) and avirulent (Bartha) strain. Comparing ADV-YS with a standard strain of Kaplan in the maps of restriction enzymes, following major respects were identified: (i) disappearance of BamHI restriction site between the first and second BamHI segments, (ii) creation of the BamHI restriction site in the fifth segment, and (iii) generation of the BglII site in the unique short (US) region. The genome of ADV-YS also contains a type 2 herpesvirus DNA molecule (in which the US region only inverts itself relative to the unique longregion) like all other ADV strains except Norden strain(type3), analyzed up to date. The size of the ADV genome estimated from the sizes of the restriction enzyme fragments, was approximately 145.3 kb (BamHI) or 145.4 kb (BglII). BamHI enzyme cleavage patterns were compared among the five Korean ADV isolates: Yangsan, Yongin, Dangjin, Jincheon and Iksan strains. Difference either in the number or in the size of the DNA fragments, suspected regions of termini of IR and TR, could be detected among all five strains.

A Study of the Impact of Sense of Values on Select Stores and Satisfaction - Centered on Eyeglasses Stores - (가치관이 점포선택과 만족도에 미치는 영향에 관한 연구 - 안경원을 중심으로 -)

  • Lee, Jung-Kyu;Cha, Jung-Won
    • Journal of Korean Ophthalmic Optics Society
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    • v.19 no.4
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    • pp.419-427
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    • 2014
  • Purpose: We propose an effective marketing strategy for eyeglasses stores on the basis of surveys about the relationships between customers' social values, customer satisfaction and selecting glasses store. Methods: All 245 surveys were conducted about customers had visited the eyeglasses store located in Seoul and Northern Gyeonggi-do regions from January 1 to June 30, 2014, and it were analyzed by SPSS v.20.0 statistical package program. Results: The order of customers' social values factors which have an influence on 'Selecting Store' is "Society oriented", "Ability oriented", "Freedom oriented" and "Happiness oriented". Also, the order of customers' social values factors which have an influence on satisfaction is "Society oriented", "Ability oriented". In addition, Customer Satisfaction is affected by Service, one of the factor of 'Selecting Store' factors. Conclusions: Each type of marketing activities should be conducted for customers by their various values. The best plan to elicit good reactions from customers is meeting them through "Society-oriented" factors. Meeting with customers through the "Ability-oriented" factors is second only to the best option. It is shown that the method to increase the satisfaction for customers' return visit is to manage 'Service factors' well.

A Study on the Assessment of Standard Wage System for Forestry Workers in Korea (임업기능인 임금조사를 통한 직종별 기준임금 산정에 관한 연구)

  • Han, Sang-Kyun;Han, Han-Sup;Woo, Hee-Sung;Choi, Byoung-Koo;Cho, Min-Jae;Cha, Du-Song
    • Journal of Korean Society of Forest Science
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    • v.104 no.4
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    • pp.632-639
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    • 2015
  • Working in the forest would require a wide range of skills and experience for specific tasks which involve with a high level of risks to worker's safety. However, there has been a concern on the current standard wage system for forest workers because it does not effectively reflect the characteristics of typical working conditions in the forest. In addition, the current standard wages for forestry workers was estimated based on the construction industry's wage system. Therefore, the purpose of this study is to assess a current wage system through the mail survey method and to develop a new wage system for forest worker which effectively reflects skill sets and experience required for successful completion of the work in the forest. We mailed the survey questionnaire consisting of 19 questions to 659 forest workers and received 188 responses resulting in a 28.5% response rate. The results showed that the current average optimal wages of forest worker, special worker and feller were 97,680won/day, 127,559won/day and 152,403won/day, respectively though there were variations depending on the regions. In developing the new standard wage system, this study suggest the current work types(worker, special worker and feller) could be divided into 5 work types (forest-environment workers, forest operations in beginner, forest operations in intermediate, forest operations in advanced and forest equipment operator) reflecting specialty of forest operation thereby stabilizing the new wage system for forest workers.

Food Behaviors and Food Preferences of the Teenagers in Urban, Fishing, and Rural Areas (일부 농어촌과 도시 청소년들의 식행동 특성 및 식품기호도)

  • 김말분;이연경;이혜성
    • Journal of the East Asian Society of Dietary Life
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    • v.7 no.3
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    • pp.341-354
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    • 1997
  • The purpose of this study was to investigate the food behaviors and food preferences of teenagers by regional groups. The subjects were composed of 365 third grade students in middle schools from urban, rural and fishing areas in Kyungpook province. The data was collected by using a survey questionnaire. The results of the study are summarized as follows: Eating habits of 21-47% of the subjects, according to the regions, were poor and female students in large city showed the best eating habits while female students in fishing village showed the poorest eating habits. Out of the three regional groups studied, the subjects from the fishing villages had the highest rates of skipping meals and eating between meals. Out of all the people investigated, thirty to forty percent had unbalanced diets, the highest percentage coming from the urban areas and the lowest from the fishing villages. All together an allergic reaction to food was the highest in the fishing villages' subjects and female students were more sensitive compared to the male students. Students from the urban area had higher frequency of symptoms of lactose intolerance. Upon the investigation of the subjects 'food preferences, they preferred boiled rice to any other rice as a staple food. Cha jang myun was the most preferred out of all noodles and breads. It was found that teenagers preferred one course meal such as fried rice to a rice-centered regular meal. Out of all various meat soups, beef soup and beef-rib soup were preferred the most. As for subsidiary foods the most preferred were soybean paste stew and cuttlefish stew which they ate daily. There was a very high preference for fried foods, the most favored being pork cutlet followed by fried vegetables, sweet potatoes, and minced meat. The most preferred cooked vegetables were bean sprouts and seasoned cucumber. Seasoned cubed radish roots were the most preferred kind of kimchi. For desserts there was a very high preference for the following fruits: apples, plums, strawberries, and oranges. For beverages the most popular choice were chocolate drinks followed by orange juice, apple juice, milk and tomato juice. According to these investigations, most teenagers liked daily foods which they are used to. The study showed that on the whole the teenagers had poor eating habits. A problem that needs immediate attention is that female students and students from the fishing villages have a high rate of skipping meals. It is expected that the results of this food preference test of teenagers could be useful in the meal planning of middle school lunch programs in the future.

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An Algorithm for Segmenting the License Plate Region of a Vehicle Using a Color Model (차량번호판 색상모델에 의한 번호판 영역분할 알고리즘)

  • Jun Young-Min;Cha Jeong-Hee
    • Journal of the Institute of Electronics Engineers of Korea CI
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    • v.43 no.2 s.308
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    • pp.21-32
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    • 2006
  • The license plate recognition (LPR) unit consists of the following core components: plate region segmentation, individual character extraction, and character recognition. Out of the above three components, accuracy in the performance of plate region segmentation determines the overall recognition rate of the LPR unit. This paper proposes an algorithm for segmenting the license plate region on the front or rear of a vehicle in a fast and accurate manner. In the case of the proposed algorithm images are captured on the spot where unmanned monitoring of illegal parking and stowage is performed with a variety of roadway environments taken into account. As a means of enhancing the segmentation performance of the on-the-spot-captured images of license plate regions, the proposed algorithm uses a mathematical model for license plate colors to convert color images into digital data. In addition, this algorithm uses Gaussian smoothing and double threshold to eliminate image noises, one-pass boundary tracing to do region labeling, and MBR to determine license plate region candidates and extract individual characters from the determined license plate region candidates, thereby segmenting the license plate region on the front or rear of a vehicle through a verification process. This study contributed to addressing the inability of conventional techniques to segment the license plate region on the front or rear of a vehicle where the frame of the license plate is damaged, through processing images in a real-time manner, thereby allowing for the practical application of the proposed algorithm.

Study on the Measurement of TMP Pumping Speed (터보분자펌프(TMP) 배기속도 측정에 관한 고찰)

  • Kang, S.B.;Shin, J.H.;Cha, D.J.;Koh, D.Y.;Cheung, W.S.;Lim, J.Y.
    • Journal of the Korean Vacuum Society
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    • v.19 no.4
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    • pp.249-255
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    • 2010
  • Methods of the characteristics evaluation of turbo-molecular pumps (TMP) are well-defined in the international measurement standards such as ISO, PNEUROP, DIN, JIS, and AVS. The Vacuum Center in the Korea Research Institute of Standards and Science (KRISS) has recently designed, constructed, and established the integrated characteristics evaluation system of TMPs based on the international documents by continuously pursuing and acquiring the reliable international credibility through measurement perfection. The measurement of TMP pumping speed is normally performed with the throughput and orifice methods dependent on the mass flow regions. However, in the UHV range of the molecular flow region, the high uncertainties of the gauges, mass flow rates, and conductance are too critical to precisely accumulate reliable data. In order to solve the uncertainty problems of pumping speeds in the UHV range, we introduced a SRG with 1% accuracy and a constant volume flow meter (CVFM) to measure the finite mass flow rates down to $10^{-1}$ Pa-L/s with 3% uncertainty for the throughput method. In this way we have performed the measurement of pumping speed down to $10^{-4}$ Pa with an uncertainty of less than 6% for a 1000 L/s TMP. In this article we suggest that the CVFM has an ability to measure the conductance of the orifice experimentally with flowing the known mass through the orifice chambers, so that we may overcome the discontinuity problem encountering during introducing two measurement methods in one pumping speed evaluation sequence.