• Korean Chemical Engineering Research
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• v.57 no.1
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• pp.85-89
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• 2019

Cell-free protein synthesis utilizes the translational machinery in a cell extract. Unlike the conventional cell-based expression methods, not being affected by the conditions for cell growth, cell-free protein synthesis enables flexible manipulation of individual factors affecting the efficiency protein biosynthesis. However, the high cost and low stability of the energy sources to regenerate ATP have limited the use of cell-free synthesis for large-scale production of recombinant proteins. One of the approaches to address this problem is to use glucose as an alternative energy source to regenerate ATP through the glucose-metabolizing pathways in a cell extract. In this study, in an attempt to improve the efficiency of ATP regeneration by reinforcing oxidative phosphorylation process, we supplemented with cellular lipids to a glucose-fueled reaction mixture for cell-free protein synthesis. As a result of the lipid supplementation, the productivity of chloramphenicol acetyltransferase in a cell-free synthesis system using glucose increased more than 6 fold compared to when the lipid was not supplemented.

• Clean Technology
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• v.24 no.4
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• pp.301-306
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• 2018

Recently, due to the increase in the fine dust, regulations on PM generated from diesel cars are strengthened. There is a growing interest in diesel particulate filters (DPFs), a post-treatment device that removes exhaust gases from diesel vehicles. Therefore, one of the enhancements of the DPF efficiency is to reduce the pressure drop in the DPF, thereby increasing the efficiency of the filter and regeneration. In this study, the effect of cell density, channel shape, wall thickness, and inlet channel ratio of 5.66" SiC and Cordierite DPF on the pressure drop in DPF was investigated using ANSYS FLUENT simulator. As a result of the experiment, the pressure drop was smaller at 300 CPSI than 200 CPSI, and the anisotropy and O / S cell showed less than Isotropy by pressure drop of about 1,000 Pa. As the porosity increased by 10% the pressure drop was reduced by about 300 Pa and as the wall thickness increased by 0.05 mm, the pressure drop was increased by about 500 Pa.

• Journal of Korean Society of Water and Wastewater
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• v.21 no.5
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• pp.539-549
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• 2007

To establish effective and prompt measures for energy conservation in public wastewater treatment plants in Korea, energy consumption rates in 233 utilities in 9 provinces and 7 metropolitan cities are investigated and compared to the rest of the world. Mean load factor for wastewater treatment utilities is 74.9% and those for influent pumps and aeration blowers are 56.2% and 61.0%, respectively. Mean electrical energy usages as the key performance indicators are $0.243kWh/m^3$ for overall sewage treatments and 2.07 kWh per unit kg BOD removal. Digester gas as one of major byproducts in the process amounts to $382,000m^3/day$ nationwide. While major part of the digester gas is used for sludge heating, only 7.3% of the gas is utilized for electricity generation. Both efficiencies for BOD removal and digestion gas generation are considerably lower than those in USA and EU utilities due to low concentration of organic material in influent wastewater. Such low energy regeneration, in turn, results in significantly higher energy consumption in Korean plants, compared to that in USA and EU ones.

• Transactions of the Korean hydrogen and new energy society
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• v.23 no.1
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• pp.83-92
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• 2012

Syngas combustion characteristics of mass produced oxygen carrier particle (OCN706-1100) were investigated in a pressurized fluidized bed reactor using simulated syngas and air as reactants for reduction and oxidation, respectively. The oxygen carrier showed high fuel conversion, high $CO_2$ selectivity, and low CO concentration at reduction conditions and no NO emission at oxidation conditions. Moreover, OCN706-1100 particle showed good regeneration ability during successive reduction-oxidation cyclic tests up to the 10th cycle. Fuel conversion and $CO_2$ selectivity decreased and CO emission increased as temperature increased. These results can be explained by trend of calculated equilibrium CO concentration with temperature. However, fuel conversion and $CO_2$ selectivity increased and CO emission decreased as pressure and gas residence time increased.

• Transactions of the Korean hydrogen and new energy society
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• v.23 no.2
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• pp.173-182
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• 2012

Effects of temperature, pressure, and gas residence time on methane combustion characteristics of mass produced oxygen carrier particle (OCN706-1100) were investigated in a pressurized fluidized bed reactor using methane and air as reactants for reduction and oxidation, respectively. The oxygen carrier showed high fuel conversion, high $CO_2$ selectivity, and low CO concentration at reduction condition and very low NO emission at oxidation condition. Moreover OCN706-1100 particle showed good regeneration ability during successive reduction-oxidation cyclic tests up to the 10th cycle. Fuel conversion and $CO_2$ selectivity decreased and CO emission increased as temperature increased. These results can be explained by trend of calculated equilibrium CO concentration. However, $CO_2$ selectivity increased as pressure increased and fuel conversion increased as gas residence time increased.

• Proceedings of the Korean Radioactive Waste Society Conference
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• 2003.11a
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• pp.701-706
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• 2003

A study on the electrosorption of U(VI) onto porous activated carbon fibers (ACFs) was performed to treat uranium-containing lagoon sludge. Effective U(Ⅵ) removal is accomplished when a negative potential is applied to the activated carbon fiber(ACF) electrode. For a feed concentration of 100mg/L, the concentration of U(VI) in the cell effluent is reduced to less than 1mg/L. The adsorbed uranium could be deserted from the ACF by passing a 1M NaCl solution through the cell and applying a positive potential onto the electrode. The regeneration of ACF from the cycling experiments was confirmed.

• Korean Chemical Engineering Research
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• v.43 no.4
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• pp.537-541
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• 2005

A bubbling fluidized bed reactor was used to study $CO_2$ capture from flue gas using a sodium-based dry regenerable sorbent, sorbA which was manufactured by Korea Electric Power Research Institute. A dry sorbent, sorbA, consists of $Na_2CO_3$ for absorption and supporters for mechanical strength. $CO_2$ capture was effective in the lower temperature range of $50-70^{\circ}C$, while regeneration occurred in the range of $120-300^{\circ}C$. To increase initial $CO_2$ removal, some amount of steam was absorbed in the sorbents before injecting simulated flue gas. It was possible to remove most $CO_2$ for 1-2 minutes at $50^{\circ}C$ and residence time of 2 seconds with steam pretreatment. Little or no reduction in initial reaction rate and capture capacity was observed in multicycle tests. The carbonated and regenerated sorbent samples were analyzed by NMR to confirm the extent of reaction. The results obtained in this study can be used as basic data for the scale-up design and operation of the $CO_2$ capture process with two fluidized bed reactors.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.36 no.1
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• pp.65-74
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• 2016

This study mainly focuses on urban regeneration project as a countermeasure to resolve climate change issues by analyzing the carbon-reduction effect of Jeonju test-bed cases. First, an urban regeneration project is designed for city, Jeonju by analyzing its environmental problems and potential improvement. Then, carbon emission and reduction amounts are evaluated for different businesses and scenarios. Carbon emission sources are classified according to a standard suggested by IPCC, and the emissions are calculated by various standard methods. The result shows that carbon emission amount in Jeonju test-bed is 102,149 tCO2eq. The fact that 70% of the emission from energy sector originates from buildings implies that urban regeneration projects can concentrate on building portions to effectively reduce carbon emission. It is also projected carbon emission will decrease by 3,826tCo2eq in 2020 compared to 2011, reduction mainly based on overall population and industry shrinkage. When urban regeneration projects are applied to 5 urban sectors (urban environment, land use, green transportation, low carbon energy, and green buildings) total of 10,628tCO2eq is reduced and 4,857tCO2 (=15.47%) when only applied to the green building sector. Moreover, different carbon reduction scenarios are set up to meet each goal of different sectors. The result shows that scenario A, B, and C each has 5%, 11%, and 15% of carbon reduction, respectively. It is recommended to apply scenario B to achieve 11% reduction goal in a long term. Therefore, this research can be a valuable guideline for planning future urban regeneration projects and relative policies by analyzing the present urban issues and suggesting improvement directions.

• Proceedings of the KSR Conference
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• 2011.10a
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• pp.2998-3006
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• 2011

This paper is about the relations between the electric multiple unit and ESS(Energy Storage system, here after) applying bi-direction Buck-Boost Converter. ESS may be possible to suppress the line voltage drops and the power loading fluctuations and to save the regeneration power lapses. This paper confirm for simulation that ESS reusing regenerative power generated during braking in train. Also, presents the influence of regenerative power variation of train and catenary acceptability for energy saving obtained by applying the ESS to Daejeon Metro line 1.

• Proceedings of the KIPE Conference
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• 1997.07a
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• pp.14-20
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• 1997

Due to several advantages of Pulse Width Modulated(PWM) Converter, such as unity power factor with low-harmonics and energy regeneration, PWM converter has been widely used in industrial application. In every application of energy conversion equipment, the design and implementation must be carried out considering performance and cost. High quality with low cost is the best choice for energy conversion equipment. High dc link voltage can reduce inverter and motor side losses and system dimension compare to low dc link voltage. Analog controller can make PWM converter cheaper without considerable degradation of the performance than digital controller. This paper shows the simplified analog controller-for 600V dc link voltage using stationary reference frame control and the simulation results.