• Title/Summary/Keyword: regeneration energy

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Efficient plant regeneration from immature embryo cultures of Jatropha curcas, a biodiesel plant

  • Varshney, Alok;Johnson, T. Sudhakar
    • Plant Biotechnology Reports
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    • v.4 no.2
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    • pp.139-148
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    • 2010
  • Jatropha curcas L. (Physic nut) is a commercially important non-edible oil seed crop known for its use as an alternate source of biodiesel. In order to investigate the morphogenic potential of immature embryo, explants from four developmental stages were cultured on medium supplemented with combinations of auxins and cytokinins. It was found that the size of embryo is critical for the establishment of callus. Immature embryos (1.1-1.5 cm) obtained from the fruits 6 weeks after pollination showed a good response of morphogenic callus induction (85.7%) and subsequent plant regeneration (70%) with the maximum number of plantlets (4.7/explant) on Murashige and Skoog's (MS) medium supplemented with IBA (0.5 $mg\;l^{-1}$) and BA (1.0 $mg\;l^{-1}$). The above medium when supplemented with growth adjuvants such as 100 $mg\;l^{-1}$ casein hydrolysate + 200 $mg\;l^{-1}$ L-glutamine + 8.0 $mg\;l^{-1}$ $CuSO_4$ resulted in an even higher frequency of callus induction (100%). Plant regeneration (90%) with the maximum number of plantlets (10/explant) was achieved on MS medium supplemented with 500 $mg\;l^{-1}$ polyvinyl pyrrolidone + 30 $mg\;l^{-1}$ citric acid + 1 $mg\;l^{-1}$ BA + 0.5 $mg\;l^{-1}$ Kn + 0.25 $mg\;l^{-1}$ IBA. It was observed that plantlet regeneration could occur either through organogenesis of morphogenic callus or via multiplication of pre-existing meristem in immature embryos. The age of immature embryos and addition of a combination of growth adjuvants to the culture medium appear to be critical for obtaining high regeneration rates. Well-developed shoots rooted on half-halfstrength MS medium supplemented with 0.5 $mg\;l^{-1}$ IBA and 342 $mg\;l^{-1}$ trehalose. The rooted plants after acclimatization were successfully transferred to the field in different agro-climatic zones in India. This protocol has been successfully evaluated on five elite lines of J. curcas.

The Establishment of Tumor Necrosis Factor Receptor-associated Protein1 (TRAP1) Transgenic Mice and Severe Fat Accumulation in the Liver of TRAP1 Mice during Liver Regeneration

  • Im, Chang-Nim;Zheng, Ying;Kim, Sun Hye;Huang, Tai-Qin;Cho, Du-Hyong;Seo, Jeong-Sun
    • Interdisciplinary Bio Central
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    • v.5 no.4
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    • pp.9.1-9.7
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    • 2013
  • Introduction: Tumor necrosis factor receptor-associated protein 1 (TRAP1) is a mitochondrial heat shock protein (HSP), which belongs to HSP90 family. It plays important roles in regulating mitochondrial integrity, protecting against oxidative stress, and inhibiting cell death. Recent studies suggest that TRAP1 is linked to mitochondria and its metabolism. In this study, we established TRAP1 transgenic mice and performed partial hepatectomy (PH) on wild-type (WT) and TRAP1 transgenic mice to investigate the function of TRAP1 during liver regeneration. Results and Discussion: We found that TRAP1 was highly expressed in liver as well as kidney. In addition, liver regeneration slightly decreased together with increased fatty liver and inflammation at 72 hr after PH in TRAP1 transgenic mice compared with WT control group mice. Concomitantly, we observed decreased levels of p38 protein in TRAP1 transgenic mice compared with WT control group mice. These results suggest that TRAP1 plays a critical role in liver energy balance by regulating lipid accumulation during liver regeneration. Conclusions and Prospects: To our knowledge, we reported, for the first time, that liver regeneration slightly reduced together with increased fat accumulations after PH in TRAP1 transgenic mice compared with WT control group mice. Concomitantly, we observed decreased levels of p38 protein in TRAP1 transgenic mice compared with WT control group mice. Overexpression of TRAP1 might affect liver regeneration via disturbing mitochondrial function leading to fatty liver in vivo.

Spent-GAC Regeneration Using Variable Frequency Sono-Fenton Oxidation (가변 주파수 Sono-Fenton 산화를 이용한 Spent-GAC 재생기술)

  • Joo, Soobin;Lee, Sangmin;Kim, Hyungjun;Shim, Intae;Kim, Heejin
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.43 no.4
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    • pp.449-458
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    • 2023
  • As an adsorption technology for dissolved organic matter, the adsorption capacity of granular activated carbon, GAC, can be applied, but activated carbon whose adsorption capacity is significantly reduced by use is inevitably replaced or regenerated. However, due to the economics of replacement cost, thermal regeneration method is used commercially, but high energy cost and loss of activated carbon occur under high temperature conditions above 800℃. In this study, the Sono-Fenton method, a multi-oxidation technology that combines Fenton oxidation and ultrasonic oxidation, was applied to improve the regeneration efficiency of spent GAC used to treat dissolved organic matter in combined sewer overflows (CSOs), and the regeneration efficiency of spent GAC by oxidant and ultrasonic frequency was investigated. In the applied Sono-Fenton treatment, the highest regeneration efficiency of 68.5% was obtained under the regeneration conditions of Fe2+ 10 mmol/L, H2O2 concentration 1,000 mmol/L, ultrasonic treatment time of 120 min, and ultrasonic frequency of 40 kHz. And similar efficiency was also obtained at 750 kHz, while ultrasonic waves of other frequencies had poor regeneration efficiency, and the magnitude of frequency and GAC regeneration efficiency did not show a linear relationship. In the case of continuous operation of the GAC adsorption tower with CSOs prepared by diluting raw sewage, about 700 hours of operation without regeneration was possible, and as a result of applying one Sono-Fenton treatment, 40-70% CODcr removal efficiency was obtained during a total of 1,000 hours of GAC adsorption operation.

A Study on the Regeneration of SCR Catalyst Deactivated by Unburned Carbon Deposition (탄소침적으로 피독된 탈질 촉매의 재생에 관한 연구)

  • Moon, Seung-Hyun;Lee, Seung-Jae;Ryu, In-Soo
    • Journal of Korean Society of Environmental Engineers
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    • v.32 no.10
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    • pp.928-935
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    • 2010
  • A bag filter system was partially burnt down during a trial run of waste wood incineration boiler. This brought about unburned hydrocarbon which caused a rapid deactivation of low temperature SCR catalyst set up in two stage after the bag filter. The deactivated catalyst was investigated in order to trace the origin by several characterization methods such as XRD, EDX, BET, TGA, SEM. The deactivated catalyst was regenerated by different methods such as acid washing, water washing in ultrasonication, and calcination treatment under air condition. It is found the calcination treatment under air condition at $450^{\circ}C$ for 2 hours to be the best regeneration method. The catalytic activity was measured in the form of 2 cm ${\times}$ 2 cm ${\times}$ 10 cm (catalyst weight 10 g) honeycomb type. A deNOx efficiency of the regenerated catalyst showed 100% at $180^{\circ}C$ which is the same level of fresh one.

A Study on the Regeneration Efficiency of the Electric Forklift Using the Variable Hydraulic Motor (가변 유압모터를 이용한 전동지게차 리프트회생 효율에 관한 연구)

  • Park, Yong Soo;Yu, Ying-Xiao;Yun, Jin Su;Do, Tri Cuong;Han, Sung Min;Shin, Jung Woo;Yu, Choong Mok;Ahn, Kyoung Kwan
    • Journal of Drive and Control
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    • v.17 no.3
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    • pp.26-32
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    • 2020
  • In modern society, the energy-saving problem of industrial vehicles is economically and environmentally critical. Energy savings using the potential energy of forklifts are one of the viable solutions to resolving this problem. The basic concept of this study is to operate the hydraulic motor and recharge the battery using the flow rate from the cylinder when loading heavy objects and lowering the fork. To save energy, the torque and rotational speed of the generator should be optimized according to the load and descent speed to increase efficiency. To this end, we propose a system that optimizes energy saving efficiency by controlling the swashplate angle of the variable hydraulic motor through the GA(Genetic-Algorithm). The results were verified by building and comparing fixed motor models and variable motor models using the AMEsim. The results of the study show that the proposed optimized swashplate angle increases the energy saving efficiency by approximately 6%-8%, depending on the working conditions.

The Effect of Plant Growth Regulators on Callus Induction and Shoot Regeneration from the Dwarf Type Variety, Hibiscus syriacus L.var. Ggoma (왜성 변이 품종 '꼬마' 무궁화의 캘러스 유도 및 지상부 형성에 식물생장조절물질이 미치는 영향)

  • Lee, Ji Yeon;Kang, Eun Jeong;Kim, Sang Hoon;Kim, Dong Sub;Kim, Jin-Baek;Ha, Bo-Keun;Kang, Si-Yong
    • Journal of Radiation Industry
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    • v.5 no.3
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    • pp.231-236
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    • 2011
  • 'Ggoma' is a new Hibiscus dwarf type variety developed by gamma irradiation at the Korea Atomic Energy Research Institute (KAERI). This study was conducted to determine the best optimum cultural callus formation and shoot regeneration condition. Sterilized leaf tissues were cultured on MS (Marashige and skoog's) medium containing 3% sucrose, 0.8% agar with different concentration and combination of TDZ, 2, 4-D, KT, BA, and 2iP for 4 weeks in vitro culture. MS medium containing 2,4-D $0.1mg\;l^{-1}$ and BA $0.5mg\;l^{-1}$ were most effective on callus formation and growth. After 4 weeks, callus was transferred on BA (0.5, 1, $1.5mg\;l^{-1}$) and TDZ (0.1, 0.2, $0.3mg\;l^{-1}$) for shoot formation. The best condition for inducing the shoot from callus was BA $1.5mg\;l^{-1}$ and TDZ $0.3mg\;l^{-1}$. This result will be useful for the rapid multiplication of Hibiscus syriacus L.var. Ggoma.

A Study on Effect of Applying Energy Storage System on SeoulMetro Line 2 (에너지저장시스템의 서울메트로 2호선 적용 효과에 관한 연구)

  • An, Cheon-Heon;Lee, Han-Min;Kim, Gil-Dong;Lee, Hi-Sung
    • Proceedings of the KSR Conference
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    • 2009.05a
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    • pp.966-971
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    • 2009
  • The recent environmental protection trend requires more strict energy saving, therefore every transportation system should reduce energy consumption to the minimum value. High-efficiency operation system, energy saving and CO2 emissions shall be addressed as important issue in railway system. These issues are the most essential factors of railway, compared to major public transportation system. Recently, saving energy in the electric railway system has been studied. For such new energy saving, the Energy storage system is considered for saving energy. Energy saving is possible by efficient use of regenerated energy. Regenerated energy is recycled amongst vehicles by mean of charge and discharge corresponding to powering and braking of electric vehicle operations. This energy saving contributes to cut CO2 to reduce greenhouse gas emissions. Recycling regenerated energy demonstrate significant effect on peak cut of consumption energy in railway substation. Absorption of excess energy avoids regeneration failure due to high traction voltage. This paper presents effects by applying the energy storage system to SeoulMetro Line 2.

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Effects of Operating Variables on Sorption Capacity of CO2 Absorbents for SEWGS Process (SEWGS 공정용 CO2 흡수제들의 흡수능력에 미치는 조업변수들의 영향)

  • Ryu, Ho-Jung;Kim, Hyo-Sung;Lee, Seung-Yong;Lee, Dong-Ho;Kim, Jae-Chang
    • Korean Chemical Engineering Research
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    • v.50 no.6
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    • pp.994-1001
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    • 2012
  • The Effects of operating variables on reactivity of two $CO_2$ absorbents (PKM1-SU and P4-600) for SEWGS process were investigated in a pressurized fluidized bed reactor. For both $CO_2$ absorbents, $CO_2$ sorption capacity decreased as the number of absorption-regeneration cycles increased. PKM1-SU absorbent represented higher $CO_2$ sorption capacity than that of P4-600 absorbent. However, P4-600 absorbent represented better performance than PKM1-SU absorbent from the view points of regeneration temperature and regeneration rate. For PKM1-SU absorbent, $CO_2$ sorption capacity increased as the steam concentration increased. However, $CO_2$ sorption capacity increased initially as the steam concentration increased from 5% to 10%, but maintained thereafter for P4-600 absorbent. For both $CO_2$ absorbents, $CO_2$ sorption capacity increased as the final regeneration temperature increased. For PKM1-SU absorbent, $CO_2$ sorption capacity increased as the pressure increased and the increment tendency was drastic at higher pressure than 15 bar.

Development of Energy Regeneration Algorithm using Electro-Hydraulic Braking Module for Hybrid Electric Vehicles (회생제동 전자제어 유압모듈을 이용한 하이브리드 차량의 에너지 회수 알고리즘 개발)

  • Yeo, H.;Kim, H.S.;Hwang, S.H.
    • Transactions of The Korea Fluid Power Systems Society
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    • v.5 no.4
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    • pp.1-9
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    • 2008
  • In this paper, an energy regeneration algorithm is proposed to make the maximum use of the regenerative braking energy for a parallel hybrid electric vehicle(HEV) equipped with a continuous variable transmission(CVT). The regenerative algorithm is developed by considering the battery state of charge(SOC), vehicle velocity and motor capacity. The hydraulic module consists of a reducing valve and a power unit to supply the front wheel brake pressure according to the control algorithm. In order to evaluate the performance of the regenerative braking algorithm and the hydraulic module, a hardware-in-the-loop simulation (HILS) is performed. In the HILS system, the brake system consists of four wheel brakes and the hydraulic module. Dynamic characteristics of the HEV are simulated using an HEV simulator. In the HEV simulator, each element of the HEV powertrain such as internal combustion engine, motor, battery and CVT is modelled using MATLAB/$Simulink^{(R)}$. In the HILS, a driver operates the brake pedal with his or her foot while the vehicle speed is displayed on the monitor in real time. It is found from the HILS that the regenerative braking algorithm and the hydraulic module suggested in this paper provide a satisfactory braking performance in tracking the driving schedule and maintaining the battery state of charge.

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Adsorption of Heavy Metal Ions onto Chemically Oxidized Ceiba pentandra (L.) Gaertn. (Kapok) Fibers

  • Chung, Byung-Yeoup;Cho, Jae-Young;Lee, Min-Hee;Wi, Seung-Gon;Kim, Jin-Hong;Kim, Jae-Sung;Kang, Phil-Hyun;Nho, Young-Chang
    • Journal of Applied Biological Chemistry
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    • v.51 no.1
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    • pp.28-35
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    • 2008
  • The physico-chemical properties of kapok fibers were altered via the combination processes of chlorite-periodate oxidation, in order to assess their efficacy as a heavy metal adsorbent. The chemically-oxidized kapok fibers were found to harbor a certain amount of polysaccharides, together with lowered lignin content. This alteration in lignin characteristics was clearly confirmed via FTIR and NBO yield. Moreover, chemically oxidized kapok fibers retained their hollow tube shape, although some changes were noted. The chemically oxidized kapok fibers evidenced elevated ability to adsorb heavy metal ions with the best fit for the Langmuir adsorption isotherm model. Three cycles of adsorption-desorption were conducted with in-between regeneration steps. Our experimental results indicated that chemically oxidized kapok fibers possessed excellent adsorption characteristics, and the modified kapok fibers could be completely regenerated with almost equimolar diluted sodium hydroxide. Pb, Cu, Cd and Zn ions evidenced adsorption rates of 93.55%, 91.83%, 89.75%, and 92.85% on the chemically oxidized kapok fibers. The regeneration efficiency showed 73.58% of Pb, 71.55% of Cu, 66.87% of Cd, and 75.00% of Zn for 3rd cycle with 0.0125N NaOH.