• Journal of Plant Biotechnology
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• v.30 no.4
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• pp.341-346
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• 2003

In an effort to optimize tissue culture conditions for genetic transformation of orchardgrass (Dactylis glomerata L.), an efficient and high-frequency plant regeneration system from seed-derived calli was established. Embryogenic calli induced on MS medium containing 3mg/L 2,4-D and 0.1mg/L BA had significantly improved regeneration ability. Plant regeneration rate was 92％ when embryogenic calli were cultured on N6 medium supplemented with 1mg/L 2,4-D and 3mg/L BA. Among three kinds of medium, MS and N6 medium were optimal for embryogenic callus induction and plant regeneration, respectively. Ho difference in callus induction frequency was observed among four cultivars of orchardgrass, however, "Roughrider" cultivar showed higher regenerability with the frequency of 61％. Addition of maltose to the regeneration medium as a carbon source dramatically increased regeneration frequency up to 69％. A short tissue culture period and high-frequency regeneration system would be beneficial for molecular breeding of orchardgrass through genetic transformation.

• Applied Biological Chemistry
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• v.50 no.4
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• pp.253-256
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• 2007

In an attempt to optimize the in vitro-regeneration conditions necessary for the genetic manipulation of tomato species, we examined 'Ailsa Graig' cultivar of Lycopersicon for regeneration ability. The basal medium used for callus formation and shoot regeneration was MS (MS + vitamin) supplemented with six combinations of zeatin 2 mg/l, zeatin 2 mg/l + IAA 0.1 mg/l, zeatin 2 mg/l + IAA 0.5 mg/l, zeatin 4 mg/l, zeatin 4 mg/l + IAA 0.1 mg/l and zeatin 4 mg/l + IAA 0.5 mg/l. When all conditions tested were considered, however, only zeatin 2 mg/l was shown to be the best in shoot regeneration. The morphological characterization from in vitro-cultured callus of Lycopersicon esculentum L. var. 'Ailsa Craig' was investigated with scanning electron microscope (SEM). The surfaces of in vitro-cultured callus had well-defined epidermal cell in condition of zeatin 2 mg/l, but those of different treatments were twisted. These results suggested that shape of callus was involved in efficiency of shoot regeneration in tomato 'Ailsa Craig'.

• Journal of Plant Biotechnology
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• v.45 no.2
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• pp.146-153
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• 2018

Lilium ledebourii Bioss is a wild species of Lilium, which grows naturally in some provinces of Iran. Previous studies on Lilium tissue culture have been linked to direct regeneration and a few studies have been conducted on indirect regeneration, which has been studied under bright conditions. In this study, for the first time in the world, all the stages of indirect regeneration (callus induction, shoot and root induction) have been studied under dark conditions. Callus formation and the regeneration levels of L. Ledebourii Bioss were examined for three replicates in an MS (Murashige and Skoog) medium with different hormonal compositions and by using a factorial experiment in the framework of a completely random plan. For callus initiation, 2,4-D and kinetin hormones were used in five and four levels, respectively, as auxin and cytokinin. Results showed that the highest percentage of the callus was found in $3{\mu}M$ of 2,4-D and $0.5{\mu}M$ of kinetin. In terms of callus wet weight, the highest amount was found in $3{\mu}M$ of 2,4-D and $0.5{\mu}M$ of kinetin. In addition, in terms of diameter, the highest amount was found in $3{\mu}M$ of 2,4-D, and $0.5{\mu}M$ of kinetin. In summary, the 2,4-D hormone had a major impact on the percentage of regeneration increase so that the best response was related to the composition of $3{\mu}M$ of 2,4-D, and $0.1{\mu}M$ of kinetin. This study contended that auxin and cytokinin can induce long shoots and roots through cell elongation in dark condition.

• Journal of the Korean Institute of Rural Architecture
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• v.15 no.3
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• pp.41-48
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• 2013

The study aims to figure out the problems of Daldongnae (hillside shantytown) in Jaman-dong, Jeonju, and to propose the countermeasures for village regeneration. Based on the survey of current conditions in the area and the case study of successful regeneration projects, this study suggests regenerating concepts for the community. The study on the current state analyzes that Jaman-dong is faced with the population decline, especially among the young, the lack of community facilities and infrastructure, and the unfavorable situation in the residential environment. The case study involves three successful Daldongnae regeneration projects: the culture-based regeneration of Cultural Village (in Gamcheon-dong, Busan), the regeneration focused on healthcare and education of Ibagugil (in Choryang-dong, Busan), and the community-led regeneration of Rainbow Village (in Dae-dong, Daejeon). The regenerating concepts for the community are as follows: utilizing the existing local community center as a gathering place for the residents, furnishing a cultural space for a growing number of visitors, adding new parking space, installing convenient facilities in order to provide better pedestrian environment for the disabled and elderly, improving street landscapes, and establishing citizen participation programs.

• Journal of Plant Biotechnology
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• v.4 no.3
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• pp.129-134
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• 2002

We report the development of a new selection system for the transformation of pepper plants by mannose. In order to achieve this, we first tested several factors related to regeneration conditions. Among the 30 inbred lines examined, line P9l5 was able to generate shoots at the highest rate from both cotyledons and hyporotyls in MS media. A dosage curve for optimizing the selection conditions was established by mixing mannose (range 0-50 g/L) and sucrose (range 0-30 g/L). The least selection pressure on shoot formation was created by a mixture of sucrose and mannose at 20 g/L and 15 g/L, respectively, and the threshold for ultimate tissue death was 50 g/L of mannose irrespective of the sucrose concentration. However, we found that mannose itself was not the sole inhibitor of pepper shoot development. High concentrations of sucrose (30 g/L) contributed additively to the inhibition of shoot formation at higher mannose concentrations. Genotype preference is a major factor that enhances regeneration ability in mannose media, as was observed in MS media. P9l5 and P410 line had high regeneration rates under mannose selection conditions in the presence of Agrobacterium infection. Different virulence levels of Agrobacterium strains did change the regeneration rates, probably due to interaction with the specificities of the inbred lines. Taken together, P9l5 offers the best pepper inbred line for transformation and we recommend a selection condition of 20 g/L of sucrose and 15 g/L or more of mannose up to 50 g/L in media.

• Journal of Microbiology and Biotechnology
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• v.1 no.4
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• pp.246-250
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• 1991

Conditions for the production and regeneration in Lactobacillus bulgaricus protoplasts were investigated. Protoplasts of L bulgaricus strains were obtained by treatment with mutanolysin and lysozyme together in a protoplast forming buffer containing 0.02 M N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid (HEPES) (pH 7.0) and 0.5 M sucrose. High protoplast yield was obtained from cells cultured in the de Man, Rogosa and Sharpe(MRS) medium at the middle to late logarithmic growth phase. Regeneration was efficiently accomplished with a complex medium containing 1% sucrose, 20 mM $MgCl_2$, 5% gelatin, and 0.5% bovine serum albumin. The frequency of regeneration of protoplasts was 10~20% after 5 days of incubation at $30^{\circ}C$.

• Microbiology and Biotechnology Letters
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• v.21 no.2
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• pp.101-106
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• 1993

The optimal conditions for the production and regeneration of L. helveticus protoplasts were examined. The protoplast formation of L. helveticus was most efficient obtained when the cells grown to mid and late logarithmic phase in MRS medium were used. The maximum number of protoplasts was obtained when lysozyme and mutanolysin were used to lysis the cell wall in 20mM HEPES buffer (pH 7.0) containing 1M sucrose. Regeneration was accomplished with a complex medium containing 10% sucrose, 10mM MaCl2, 20mM CaCl2, 5% gelatin and 0.5% bovine serum albumin. The regeneration frequency of the protoplasts was 10-20% after 5 days of incubation at 30C.

• Microbiology and Biotechnology Letters
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• v.23 no.3
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• pp.359-364
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• 1995

Two lactic strains, Leuconostoc mesenteroides Lu5 and Pediococcus pentosaceus P1 isolated from Kimchi, were used to determine the optimum conditions for protoplast formation and regeneration. The maximum protoplast formation rate was obtained with both strains at early exponential growth phase and decreased rapidly during growth phase. For P. pentosaceus P1, 30 $\mu$g/ml of lysozyme treatment was sufficient to obtain over 90% of protoplast formation and 300 $\mu$g/ml for L. mesenteroides Lu5, showing great difference in sensitivity of these strains to lysozyme. For both strains, best results were obtained at pH 7, using 0.5 M sucrose as osmotic stabilizer. For regeneration of protoplast, the highest regeneration rate was obtained after 15 minutes of lysozyme treatment and declined drastically with prolonged digestion.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.7 no.5
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• pp.788-792
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• 2006

The test was conducted on an 8000cc heavy-duty turbo-charged heavy-duty diesel engine on which continuous regeneration DPF was installed in order to investigate regeneration characteristics fur DPF and engine performance under conditions of standard (430ppm) or ultra low sulfur diesel (50ppm) and the results were compared with each other. Exhaust emissions, CO, HC, NOx, PM and soot were investigated carefully and tested under D-13 and D-3 modes.

• The Korean Journal of Ecology
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• v.21 no.4
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• pp.389-399
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• 1998

this study was focused on the effects of fire on spatial change of vegetation landscape in rural region. Fire types recognized as erown fire, severe surface fire and light surface fire in order of increasing intensity were described in a fire map. GIS was introduced to understand the relationship between fire types and topographic conditions or vegetation types. We also investigated land-use type and regeneration strategies after burning. Fire intensity depended on topographic conditions and vegetation types. Special land-use type in this area was collection of edible mushroom (Tricholoma matsutake). Mushrooms had been obtained from Pinus densiflora forests existing as edaphic climax or managed artificially. Regeneration strategy in burned areas was to make sprouts from burned oak stumps. A higher density and growth rate of sprouts, as compared to those on unburned areas, facilitated vegetation succession from P. densiflora forest to oak forest and consequently led to change of landscape pattern.