• Proceedings of the Korean Society of Applied Pharmacology
• /
• 1996.04a
• /
• pp.208-208
• /
• 1996

In this work we investigated coupling of the m2 and m4 subtypes of muscarinic acetylcholine receptors expressed in chinese hamster ovary (CHO) cells to activation of neuronal nitric oxide synthase (nNOS). Stimulation of guanylate cyclase activity in detector neuroblastoma cells was used as an index of generation of nitric oxide (NO) in CHO cells. The agonist carbachol induced marked time and concentration-dependent enhancement of the activity of nNOS at m2 receptors. In sharp contrast, the response in CHO cells transfected with the m4 receptor gene was similar in magnitude to that observed in non-transfected cells, suggesting lack of significant coupling of m4 muscarinic receptors to NO signaling. This novel observation of functional divergence of the two muscarinic receptor subtypes at the level of activation of nNOS is quite intriguing, in light of the currently accepted dogma that they belong to the same functional class. This functional selectivity was not due to differential effects on intracellular Ca$\^$2+/ concentration, since activation of both subtypes of muscarinic receptors produced a comparable, albeit quite small, Ca$\^$2+/ signal. Taken together, our present data strongly suggest that the generally assumed functional equivalence of m2 and m4 muscarinic receptors should be carefully reexamined. These data also suggest the presence of alternate mechanisms of activation of nNOS, which might be operative in the absence of large changes in the concentration of cellular Ca$\^$2+/. The latter mechanisms are expected to be activated by m2, but not m4 muscarinic receptors. Both sets of findings are quits important in regards to refining the functional classification of muscarinic receptor subtypes and the cellular mechanisms of activation of NOS.

• Proceedings of KOSOMES biannual meeting
• /
• 2006.05a
• /
• pp.85-92
• /
• 2006

Crude oil is complex mixture if thousands of different organic compound formed from a variety of organic materials that are chemically converted under differing geological conditions over long periods of time. Also oil composition varies according to crude source, refining, processing, handling and storage. The oil fingerprint method is application if specific knowledge of petrochemicals and use if sophisticated analytical equipment and techniques to identify the source(s) if oil pollution. KCG currently utilizes four primary analytical techniques: Gas Chromatography (GC), Fluorescence Spectroscopy(FL), Infrared Spectroscopy(IR) and Gas Chromatography mass spectrometer(GC/MS). Of all these techniques, GC technique are most widely used Gas Chromatography is used as a primary analytical method because high reliableness, high separating efficiency and repeatability.

• Journal of Navigation and Port Research
• /
• v.43 no.6
• /
• pp.469-474
• /
• 2019

One of the world's major resources is crude oil, the most fundamental part of the industry. There is no place that does not use crude oil. Petroleum refining products and chemical production industrial products are produced through nearby petrochemical complexes and ports after importing crude oil. There would be a possible relationship among the petrochemical complexes and nearby regional ports working with liquid cargoes. To confirm these relations, Ulsan Port, Daesan Port, and Yeosu Gwangyang Port were selected for this study. A Vector Auto Regressive model using time series data was applied. A Unit Root Test was performed. The relationship was confirmed through the Granger and Toda Yamamoto Causality Test.

• Korean Journal of Oriental Medicine
• /
• v.18 no.3
• /
• pp.141-146
• /
• 2012

Objectives : KIOM(Korean Institute of Oriental Medicine) built up korean medical paper database and services it through information portal OASIS. The database are updated about 1,600 papers and 48,000 references annually. Because lots of manpower and time are needed to update database, it is very important to raise up efficiency and quality of it. Methods : In this paper, we implemented web based database building system utilizing pre-built OASIS' database to improve the working process, data quality and ease of management. Results : First we designed and implemented web based system to input bibliography of the paper efficiently. It raised efficiency using OASIS' paper and reference database. Second we improved the refining process using web based system to raise up data quality. And third we developed the manager functions of web based system to control and check the working process. Conclusions : If we add korean medical dictionary and link outside paper database in the future, we hope that work efficiency and data quality will be raised more. And because the database schema of OASIS system and developed system are different, we are implementing the data transformation system.

• Korean Journal of Plant Resources
• /
• v.20 no.2
• /
• pp.125-128
• /
• 2007

Plan stability production investigating 1st harvesting degree for maximum leaf quantity enlargement, is as following it summarize result that test for 3 years since 2002 allowing 4 processing such as trunk lower column department harvesting, Foliar and rhizoma growth were tendency that give protective care 1 st harvesting height is short, but there were many the number of tillering crawl, Distribution of rhizome about diameter 5mm low 58%, large rhizome's ratio was high tendency harvesting height is short. Because foliar amount is much harvesting height is short in ground department, 15% rose in soil surface harvesting since 292kg provision per 5cm harvesting 10a, The time of refining the harvest of stems and leaves before drying has reduced when the height of the harvest is low, and the 5cm harvest has decreased 30% compared to the soil surface harvest.

• Asian Pacific Journal of Cancer Prevention
• /
• v.16 no.13
• /
• pp.5191-5197
• /
• 2015

A partial response or resistance to chemotherapeutic agents is considered as a main obstacle in treatment of patients with cancer, including breast cancer. Refining taxane-based treatment procedures using adjuvant or combination treatment is a novel strategy to increase the efficiency of chemotherapy. PPM1D is a molecule activated by reactive oxygen species. whose expression is reported to modulate the recruitment of DNA repair molecules. In this study we examined the impact of arsenic trioxide on efficacy of paclitaxel-induced apoptosis in paclitaxel-resistant MCF-7 cells. We also investigated the expression of PPM1D and TP53 genes in response to this combination treatment. Resistant cells were developed from the parent MCF-7 cell line by applying increasing concentrations of paclitaxel. MTT assays were applied to determine the rate of cell survival. DAPI staining using fluorescent microscopy was employed to study apoptotic bodies. Real-time RT-PCR analysis was also applied to determine PPM1D mRNA levels. Our results revealed that combination of arsenic trioxide and paclitaxel elevates the efficacy of the latter in induction of apoptosis in MCF-7/PAC resistant cells. Applying arsenic trioxide also caused significant decreases in PPM1D mRNA levels (p<0.05). Our findings suggest that arsenic trioxide increases paclitaxel-induced apoptosis by down regulation of PPM1D expression. PPM1D dependent signaling can be considered as a novel target to improve the efficacy of chemotherapeutic agents in resistant breast cancer cells.

• Herbal Formula Science
• /
• v.14 no.1
• /
• pp.82-104
• /
• 2006

Objectives: The aim of this study is to find the therapeutic meaning of the Pyrite in herbal medication. Methods: About the origin, the component, the processing the drug, the properties and tastes of drugs, the meridian tropism, the effects, the treating disease, the contraindication and the method of administration. We have researched thirty three literatures to mention the pyrite in time sequence. Results: 1. The pyrite belongs to the metallic herbs and it consists of Iron sulfide, sometimes containing small amounts of cobalt, nickel. silver, and gold. 2. The processing the drug are the tempering, the annealing with vinegar. the refining drug with water or RADIX GLYCYRRHIZAE and et cetra, and accoring to these methods, the pyrite classified into three groups - the rough pyrite, the tempered pyrite, the annealed pyrite. 3. The properties and tastes of drugs is pungent and regular. If pyrite is well refine, it is nearly safe form the metallic virulence. the meridian tropism is mainly liver meridian. 4. From old times, pyrite has come into general use to treat the injury of muscles and tendons and bone facture because it is effective on dissipating blood stasis, alleviating pain and reunion of bone, muscles and ligament. 5. When the symptom is gone, a medicine to contain pyrite must be stoped taking. Because of pyrite's effect on regulateing Gi and promoting blood flow. Conclusions: This study showed that the pyrite is useful herb to treat the injury of muscles and tendons and bone facture.

• KSBB Journal
• /
• v.15 no.2
• /
• pp.201-207
• /
• 2000

The traditional ripening method of clay was analyzed. An advanced refining method of clay using enrichment cultures of iron r reducing bacteria was developed. After the traditional ripening, the whiteness of the clay was increased due to removal of | iron impurities by inhabitant dissilmaltien with iron reducing bacteria. Other characteristics of the refined clay such as v viscosity, plasticity, and strength were also improved by iron reducing bacteria. An advanced method of clay refinement with a anaerobic enrichment cultivation of iron reducing bacteria supplemented with an extra carbon source such as glucose was s suggested. When the clay was treated by the advanced method. the refinement time could be reduced to 1/6 of that r required by the traditional method. The physical properties of the refined clay by the advanced method were better than t those of the traditionally refined clay.

• Corrosion Science and Technology
• /
• v.7 no.6
• /
• pp.350-361
• /
• 2008

Canada's oil sands contain one of the largest reserves of oil in the world. According to recent estimates, there are nearly 180 billion barrels of oil in the Canadian oil sands trapped in a complex mixture of sand, water and clay. More than 40 companies have been currently operating or developing oil sands facilities since the first production in 1967. The process of oil sands upgrading is similar with down stream refinery, but the corrosion environment in upgrading refinery is often more severe than in the refinery because of high chlorides, mineral contents, carbonic acid, heavy viscosity and fouling, higher naphthenic acid [$NA-R(CH_{2})nCOOH$], and greater sulfur contents. Naphthenic acid corrosion (NAC) which is one of the most critical corrosion issues in up & downstream refinery plants was observed for the first time in 1920's in refinery distillation processes of Rumania, Azerbaizan (Baku), Venezuela, and California. As a first API report, the 11th annual meeting stated sources and mechanism of NAC in early 1930's. API has been developing the risk base standards, such as API RP580, 571, and Publication 581 which are based on the worst NAC damage in the world since 2000. Nevertheless not only the NAC phenomena and control in Canadian sands oil process are not much widely known but also there are still no engineering guidances for the Canadian sands oil in API standards. This paper will give NAC phenomina and materials selection guidance against NA environment in Canadian oil sands upgrading processes.

• Journal of Korean Society of Archives and Records Management
• /
• v.15 no.4
• /
• pp.49-76
• /
• 2015

The personal history of individuals directly and vividly reflects the courses of their life and the society of which they are a part. It has become a meaningful part of the social landscape today to pursue efforts to understand humans and humanity correctly and properly through the windows of private records. Notably, the significance of keeping private records on the relationship between individuals and the society of which they are a part lies in the fact that they cooperate to build a foundation on which they can prosper together. One of necessary things to be done to fully appreciate the values of such private records is to perform serious analyses on particular individuals who are behind those records. Developing and refining methods of life history research such as time-line interviews exert positive influence in setting up a direction for private documentation systems where private records are collected and maintained.