• Title/Summary/Keyword: reducing sugar content

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Extraction of Liberated Reducing Sugars from Rapeseed Cake via Acid and Alkali Treatments (산 및 알칼리 처리에 의한 유채박의 유리당 추출)

  • Jeong, Han-Seob;Kim, Ho-Yong;Ahn, Sye-Hee;Oh, Sei-Chang;Yang, In;Choi, In-Gyu
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.40 no.11
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    • pp.1575-1581
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    • 2011
  • Rapeseed cake, which is the organic waste remaining after rapeseed oil production, is readily available and considered an ecologically-friendly resource with very low cost and high dietary fiber content. This research was carried out for two reasons. First, it was done to analyze the liberated reducing sugar content of rapeseed cake. Second, it was done to investigate the effects on the sugar yield of the various concentrations of acidic and alkaline catalysts used for the hydrolysis of rapeseed cake and the concentrations of rapeseed cake in each catalyst. Several amounts of ground rapeseed cake, 0.5 g, 1 g, and 2 g, were put into 100 mL of catalysts such as sulfuric acid (0.5~2%), hydrochloric acid (0.5~2%), and sodium hydroxide (0.5~2%). Then they were hydrolyzed for 5 min at 121$^{\circ}C$. After hydrolysis, HPLC equipped with an RI detector was used to analyze liberated reducing sugars such as sucrose, glucose, galactose, fructose, and arabinose separated from rapeseed cake. The degradation rate of rapeseed cake was the highest in hydrochloric acid. As the catalyst concentrations used for hydrolysis of rapeseed cake increased, the degradation rate of rapeseed cake also significantly increased. Total reducing sugar content was the highest in hydrochloric acid, and it increased with the increase of catalyst concentrations. However, as the amount of rapeseed cake increased, the total reducing sugar content decreased, exceptionally sucrose in the case of sodium hydroxide.

Preparation of Traditional Malt-Sikhye - 2. Preparation by Malt (전통식혜제조-제 2보 엿기름에 의한 제조)

  • 안용근
    • The Korean Journal of Food And Nutrition
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    • v.12 no.2
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    • pp.170-176
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    • 1999
  • Optimum preparation conditions of Korean traditional sweet rice drink 'malt-Sikye' were 1hour of rice soaking time 30min of malt extraction time 60$^{\circ}C$ of malt extraction tem-perature 60$^{\circ}C$ of saccharification temperature 1hour of agitation interval 5hour of saccharification time 5.5 of pH 4% of malt concentration and 20% of rice content. The malt-Sikhy contained to 17.1% of total sugar 11.2% of reducing sugar o.34mg/ml of protein and 4.7${\mu}$mol/ml of amino acid. The most abundant sugar found in malt-Sikhye was maltose. The commercial amylolytic enzymes were not effec-tive in preparation malt-Sikye.

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Biochemical and Histological Charaeteristics of Inferior Red Ginseng (불량홍삼(내백삼)의 생화학적 및 조직학적 특성)

  • Do, Jae-Ho;Kim, Sang-Dal;Seong, Hyeon-Sun
    • Journal of Ginseng Research
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    • v.9 no.2
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    • pp.256-263
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    • 1985
  • In order to investigate the inferior factor of red ginseng quality, the contents of various chemical components, physico-chemical properties and arrangement state of ginseng cells were observed. Contents of total reducing sugar, reducing sugar, crude protein, crude fibre and specific gravity of inside white part of red ginseng were less than those of normal part. But differences in content of crude saponin, HPLC pattern of ginsenosides and reducing ability for DP P H(1,1-dipheny 1-2-picrylhydrazyl) between normal and inside white part of red ginseng were not found. The optical density of 1 water extract of normal part of red ginseng did not differ from that of inside white 1 part of red ginseng, but the visible and UV absorbance of acid hydrolyzate of normal red ginseng showed higher than those of inside white part of red ginseng. The differences in the internal color and tissue of normal and inside white part of red ginseng were easily found with naked eye, and by the microscopic fractography, the orangement state of ginseng cell in the inside white part of red ginseng was less dense than that in normal red ginseng.

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Optimization of Soy Sauce Production Conditions with Black Garlic Extract by Response Surface Methodology (반응표면 분석법에 의한 흑마늘 추출물이 첨가된 간장의 제조 조건 설정)

  • Shim, Hye-Jin;Kang, Min-Jung;Kim, Gyeong-Min;Lee, Chang-Kwon;Kim, Jeong-Hwan;Shin, Jung-Hye
    • Korean journal of food and cookery science
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    • v.32 no.3
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    • pp.307-315
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    • 2016
  • Purpose: The central composition design was used to optimize the mixture conditions of black garlic extract. Methods: The response surface methodology (RSM) was carried out from concentration of black garlic extract ($X_1$) and the amount of the black garlic extract ($X_2$) as independent variables, and salts ($Y_1$), reducing sugars ($Y_2$), the content of total phenolic compounds ($Y_3$) and ABTS radical scavenging activity ($Y_4$) as dependent variables. We confirmed the conditions that salinity was minimized and reducing sugar, total phenolic compounds and ABTS radical scavenging activity had maximum values through the response surface analysis. Results: All results had saddle points in originally set up conditions hence, ridge analysis was carried out for narrowing the experimental area. The minimum salt concentration was 16.03% at black garlic extract concentration of $14.84^{\circ}brix$ and contents of 9.26%. Reducing sugar content had maximum of value 7.30 g/mL at $24.58^{\circ}brix$ and contents of 8.08%. Total phenolic compounds contents and ABTS radical scavenging activity had maximum values at black garlic extract concentration of 20.33 and $25.02^{\circ}brix$. The results indicate that addition of black garlic extract contributed to increased reducing sugar, phenolic compounds contents and antioxidant activity of the soy sauce, but the salt concentration was not significantly affected. Conclusion: Based on the results of RSM, the optimum ranges of addition conditions for lowering the salt concentration and, increasing the sensory and functional ability of soy sauce were as follows: black garlic extract concentration of $15-25^{\circ}brix$ and content of 7.8-9.3%.

Study on the Sugar Composition of Non-germinated Malt (무발아(無發芽) 맥아(麥芽)의 당조성(糖組成)에 관(關)한 연구(硏究))

  • Shin, Eung-Tae;Kim, Chang-Sik
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.14 no.3
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    • pp.244-248
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    • 1985
  • This experiments was carried out to study the identification of sugar formation during malting period in non-germinated malts. The yields of non-germinated and germinated malts were 89.5% and 83.0%, respectively. The content of reducing sugar in non-germinated malts by acid and freezing treatment methods showed more increasing tendency during malting 4 days than standard method. Fructose, glucose, maltose in non-germinated malts (Malting 1) were identified by HPLC and its content were calculated as 3.9 mg/g, 52.1 mg/g, and 20.1 mg/g.

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Manufacturing Characteristics of Solid-State Fermentation Distilled Spirits Using Rice Nuruk (Fermented Starter) and Sorghum (쌀누룩과 수수를 사용한 고체발효 증류주의 제조 특성)

  • Shin, J.Y.;Jeong, D.S.;Kang, C.S.;Choi, H.S.
    • Journal of Practical Agriculture & Fisheries Research
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    • v.23 no.1
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    • pp.39-48
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    • 2021
  • In this study, characteristics of solid-state fermentation distilled spirits with nuruk (fermentation starter) and sorghum were investigated. The starch digestion activity was highest in black koji (A. usamii) at 359.15 U/g dry base, white koji (A. luchuensis) at 306.98 and yellow koji (A. oryzae) at 15.31 was followed. The pH of the solid-state fermented mash in yellow, white and black koji showed 5.09, 4.46 and 4.50 respectively with significant differences (p<0.001). The total acid content of white and black koji was 0.73% as citric acid. The alcohol content of mash prepared with yellow, white and black koji was 4.34, 4.24, 3.89% respectively. The contents of reducing sugar showed 3.28, 3.23 and 2.64%. Total sugar were 14.88, 17.84 and 17.60% respectively. The alcohol content of each distillate ranged from 44.3 to 39.9%, and the furfural content in yellow koji was 1.25 times lower than the others.

The Quality Characteristics of Wheat-Makgeolli Made from Different Cultivars and Milling Rates (국산 밀 품종 및 제분율에 따른 막걸리의 품질 특성)

  • Sim, Eun-Yeong;Lee, Seuk Ki;Woo, Koan Sik;Kim, Hyun-Joo;Kang, Chon-Sik;Kim, Si Ju;Oh, Sea-Kwan;Park, Hye-Young
    • The Korean Journal of Food And Nutrition
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    • v.29 no.5
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    • pp.777-784
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    • 2016
  • This study aimed to evaluate the quality characteristics of wheat-Makgeolli (WM), a traditional Korean cereal alcoholic drink, using three varieties of wheat, namely Jokyoung (JK), Baegjoong (BJ) and Keumkang (KK). Samples of WM brewed from 100%, 85% and 70% milling rates of the three Korean wheat cultivars were analyzed for alcohol, pH, coloring degree, total acids, soluble solid, free sugars, and organic acids. As the milling rates in wheat decreased, total sugar content in WM increased while the pH of all samples decreased. The WM exhibited 0.95~1.27% in acidity, $10.2{\sim}12.5^{\circ}Brix$ in total sugar, and 14~16% in alcohol content. The most organic acids in WM was lactic acid, ranging in all the samples from 85.3~650.3 mg%. The results showed that BJ under a 70% milling rate had the highest reducing sugar contents and 15.97% in alcohol content. The carbohydrate content increased with the milling rate of wheat. Resulting in a positive correlation between carbohydrate content of wheat and total acids, reducing sugars (p<0.001), and alcohol content (p<0.05) in WM. Total sugar content is positively correlated with alcohol and reducing sugar content (p<0.001). Considering the yield, the milling rates will be adjusted to raw material prices.

Quality Characteristics of Sikhye Prepared with Puffed Rice Powder during Saccharification (팽화미분 첨가에 따른 식혜의 품질 특성)

  • Lee, Myung-Wook;Yi, Young Hyoun
    • Korean Journal of Food Science and Technology
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    • v.44 no.5
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    • pp.553-558
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    • 2012
  • The pH, turbidity, color, brix, reducing sugar, total sugar, ketose, amino acid, protein and organoleptic characteristics of sikhye prepared with puffed rice powder (0, 25, 50, 75 and 100% (w/w)) were investigated during saccharification. A higher concentration of puffed rice powder and longer saccharification time resulted in pH reduction (p<0.05) and tended to increase turbidity. A higher Hunter "L" value was observed with higher puffed rice powder content, except for the addition of 25% at 0 h. The Hunter "L" value peaked at hour 1 and slowly declined thereafter. Elevations in brix, reducing sugar, total sugar and sucrose were observed with higher puffed rice powder content and longer duration of saccharification (p<0.05). The sucrose content ranged from 0.79-0.86% (w/w) at hour 6, which was markedly lower than that of commercial sikhye. With time, levels of amino acid and protein increased (p<0.05). Sensory evaluation revealed moderate differences between 0% and both 75 and 100% (p<0.001), with 75 and 100% preferred over 0% (p<0.05).

Quality Characteristics of 4 Year-old Ginseng by Enzymatic Hydrolysis Conditions (4년근 인삼의 효소적 가수분해 조건에 따른 품질특성)

  • Im, Ga-Young;Ma, Jin-Yeul;Kim, Kun-Woo;Choi, Jin-Kook;Kang, Dong-Kyoon;Kwon, Tae-Ryoung;Jang, Se-Young;Jeong, Yong-Jin
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.40 no.2
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    • pp.229-234
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    • 2011
  • This study investigated quality characteristics of 4 year-old ginseng by enzymatic hydrolysis conditions to increase utilization. Ginseng was ground after steaming and was each treated with hydrolase A, B, C and D. When quality characteristics by the enzymes were examined, no significant difference was observed with pH of 5.5~5.6 and the sugar content of 4.0~4.33. The crude saponin content was the highest in ginseng treated with D, followed by B, C and A. The crude saponin, the reducing sugar and the total sugar contents increased until 0.3% (w/w) concentration in enzyme D with no significant difference by its concentration. Although active ingredients increased with time passage of hydrolysis, no significant change was found after three hours and the crude saponin content was the highest when ginseng was treated at $60^{\circ}C$. From these results, optimum conditions for 4 year-old ginseng were $60^{\circ}C$ for 3 hours with 0.3% (w/w) enzyme D, and under these conditions the reducing sugar, the total sugar and the crude saponin contents recorded 18.11, 36.21 and 4.23 mg/g, respectively. Therefore, enzymatic hydrolysis was found to be effective in increasing active ingredients of 4 year-old ginseng with various usages expected.

Studies on the Standardization of the Processing Condition of Ko-Choo-Jang(Red Pepper-Paste) (고추장 제조조건(製造條件)의 표준화(標準化)에 관(關)한 연구(硏究))

  • Yeo, Young-Keun;Kim, Ze-Uook
    • Applied Biological Chemistry
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    • v.21 no.1
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    • pp.16-21
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    • 1978
  • In order to determine optimum condition for the Ko-Choo-Jang (red-pepper-paste) processing, chemical analysis, mixed ratio, and sensory teat, resulted as follows. 1. The best nutritional value could be obtained after three hours at $60^{\circ}C$, which proved to be the optimum temperature in koji digestion of starch paste. 2. The component ratio of wheat flour to koji-powder was two to one giving the best digestion. 3. The mixing ratio of salt and red-pepper-powder and flour-koji was determined as 1 : 1.5 : 3 by sensory tests. 4. The highest reducing sugar content appeared in a digested mixture using glutinous rice as a starch source and the reducing sugar increased continuously with decreasing, total sugar content in almost every mixture of starches. 5. As a starch source, glutinous rice powder produced the highest total sugar in the beginning stages, however, after 10 day's curing produced less sugar than rice powder. 6. The order of total nitrogen content in the cured paste was recorded as wheat-flour, rice-powder, corn-powder, glutinous-powder, barley-powder, and sweet potato-starch. 7. Amino-nitrogen was increased with curing and the highest, value was observed in the case of wheat flour. 8. In sensory test, Ko-Choo-Jang made of glutinous rice-powder resulted in the best taste.

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