Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Quality Characteristics of 4 Year-old Ginseng by Enzymatic Hydrolysis Conditions

4년근 인삼의 효소적 가수분해 조건에 따른 품질특성

  • Im, Ga-Young (Center for Herbal Medicine Improvement Research, Korea Institue of Oriental Medicine) ;
  • Ma, Jin-Yeul (Center for Herbal Medicine Improvement Research, Korea Institue of Oriental Medicine) ;
  • Kim, Kun-Woo (School of Bioresource Sciences, Andong National University) ;
  • Choi, Jin-Kook (Gyeong Buk Provincial A.T.A) ;
  • Kang, Dong-Kyoon (Gyeong Buk Provincial A.T.A) ;
  • Kwon, Tae-Ryoung (Gyeong Buk Provincial A.T.A) ;
  • Jang, Se-Young (Dept. of Food Science and Technology, Keimyung University and Keimyung Foodex Co., Ltd.) ;
  • Jeong, Yong-Jin (Dept. of Food Science and Technology, Keimyung University and Keimyung Foodex Co., Ltd.)
  • Received : 2010.11.04
  • Accepted : 2010.12.23
  • Published : 2011.02.28
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Abstract

This study investigated quality characteristics of 4 year-old ginseng by enzymatic hydrolysis conditions to increase utilization. Ginseng was ground after steaming and was each treated with hydrolase A, B, C and D. When quality characteristics by the enzymes were examined, no significant difference was observed with pH of 5.5~5.6 and the sugar content of 4.0~4.33. The crude saponin content was the highest in ginseng treated with D, followed by B, C and A. The crude saponin, the reducing sugar and the total sugar contents increased until 0.3% (w/w) concentration in enzyme D with no significant difference by its concentration. Although active ingredients increased with time passage of hydrolysis, no significant change was found after three hours and the crude saponin content was the highest when ginseng was treated at $60^{\circ}C$. From these results, optimum conditions for 4 year-old ginseng were $60^{\circ}C$ for 3 hours with 0.3% (w/w) enzyme D, and under these conditions the reducing sugar, the total sugar and the crude saponin contents recorded 18.11, 36.21 and 4.23 mg/g, respectively. Therefore, enzymatic hydrolysis was found to be effective in increasing active ingredients of 4 year-old ginseng with various usages expected.

본 연구에서는 4년근 인삼의 활용도를 높이기 위하여 효소적 가수분해 조건에 따른 품질 특성을 조사하였다. 인삼은 증숙 후 분쇄하여 가수분해 효소제 A, B, C 및 D 4종에 처리에 따른 품질특성을 조사한 결과 pH 5.5~5.6, 당도 4.0~4.33으로 큰 차이가 없었다. 조사포닌 함량은 D 효소제에서 가장 높았으며, B, C 및 A 순으로 나타났다. D 효소제의 농도 0.3%(w/w)까지 조사포닌, 환원당 및 총당 함량이 증가하였으며 이후에는 농도에 따른 차이는 없었다. 가수분해시간이 경과함에 따라 유효성분이 증가하였으나 3시간 이후 큰 변화는 없었으며, $60^{\circ}C$로 처리하였을 때 조사포닌 함량이 높게 나타났다. 이상의 실험결과 D 효소제, 효소제 농도 0.3% (w/w)로 $60^{\circ}C$에서 3시간의 조건이 가장 적합한 나타났으며 이때 환원당, 총당 및 조사포닌 함량은 각각 18.11, 36.21 및 4.23 mg/g으로 확인되었다. 따라서 효소적 가수분해 방법은 4년근 인삼의 유효성분 증가에 효과적으로 다양한 활용 방법이 기대되었다.

Keywords

References

  1. Im GY, Jang SY, Jeong YG. 2010. Quality characteristics of Panax ginseng C. A. Meyer with steaming heat and wet grinding conditions. J Korean Soc Food Sci Nutr 39: 1005-1010. https://doi.org/10.3746/jkfn.2010.39.7.1005
  2. Shibata S, Tanaka O, Soma K, Iita Y, Ando T, Nakamura H. 1965. Studies on saponins and sapogenins of ginseng. Tetrahedron Lett 3: 207-213.
  3. Sotaniemi EH, Haapakoski E, Rautio A. 1995. Ginseng therapy in non-insulin-dependent diabetic patients. Diabetes Care 18: 1373-1375. https://doi.org/10.2337/diacare.18.10.1373
  4. Nam KY. 2002. Clinical applications and efficacy of Korean ginseng. J Ginseng Res 26: 111-131. https://doi.org/10.5142/JGR.2002.26.3.111
  5. Sacchio O. 1990. Clinical effectiveness of Korea ginseng on climacteric disturbances and its possible mechanism of action. J Ginseng Res 14: 162-166.
  6. Lee MJ, Kim EH, Rhee DK. 2008. Effects of Panax ginseng on stress. J Ginseng Res 32: 8-14. https://doi.org/10.5142/JGR.2008.32.1.008
  7. Choe HJ, Han HS, Park JH, Son JH, Bae JH, Seong TS, Choe C. 2003. Natural products organic chemistry: antioxidantive, phospholipase $A_2$ inhibiting, and anticancer effect of polyphenol rich fractions from Panax ginseng C. A. Meyer. J Korean Soc Agric Chem Biotechnol 46: 251-256.
  8. Hwang WI, Oh SK. 1984. A study on the anticancer activities of lipid soluble ginseng extract and ginseng saponin derivatives against some cancer cells. J Ginseng Res 8:153-166.
  9. Kwak YS, Choi KH, Kyung JS, Won JY, Rhee MH, Lee JG, Hwang MS, Kim SC, Park CK, Song KB, Han GH. 2008. Effects of high temperature heating on the some physicochemical properties of Korean red ginseng (Panax ginseng C.A. Meyer) water extract. J Ginseng Res 32: 120-126. https://doi.org/10.5142/JGR.2008.32.2.120
  10. Park CK, Kwak YS, Hwang MS, Kim SC, Do JH. 2007. Trends and prospect of ginseng products in market health functional food. Food Sci Ind 6: 30-45.
  11. Han BH, Park CK, Han YN. 1982. Degradation of ginseng under mild acidic condition. Planta Med 44: 146-149. https://doi.org/10.1055/s-2007-971425
  12. Kim MH, Lee YC, Choi SY, Cho CW, Rho JH. 2009. Characteristics of acid pre-treated red ginseng and its decoction. J Ginseng Res 33: 343-348. https://doi.org/10.5142/JGR.2009.33.4.343
  13. Yang SJ, Woo KS, Yoo JS, Kang TS, Noh YH, Lee JS, Jeong HS. 2006. Change of Korean ginseng components with high temperature and pressure treatment. J Korean Food Sci Technol 38: 521-525.
  14. Bae EA, Kim NY, Han MJ, Choo MK. 2003. Transformation of ginsenosides to compound K (IH-901) by lactic acid bacteria of human intestine. J Microbiol Biotechnol 13: 9-14.
  15. Im KS, Chung HY, Park SH, Je NK. 1995. Anticancer effect of hydrolyzed monogluco-ginsenoside of total saponin from ginseng leaf. J Ginseng Res 19: 291-294.
  16. Ko SR, Suzuki Y, Choi KJ, Kim YH. 2000. Enzymatic preparation of genuine prosapogenin, 20(S)-ginsenoside $Rh_1$, from ginsenosides Re and $Rg_1$. Biosci Biotechnol Biochem 64: 2739-2743. https://doi.org/10.1271/bbb.64.2739
  17. Kim YC, Yim JH, Rho JH, Cho CW, Rhee YK. 2007. Antioxidant activity of white ginseng extracts prepared by enzyme treatment on V79-4 cells induced by oxidative stress. J Ginseng Res 31: 203-209. https://doi.org/10.5142/JGR.2007.31.4.203
  18. Kim MJ, Lim KR, Jung TK, Yoon KS. 2007. Antiaging effects of astragalus membranaceus root extract. J Soc Cosmet Sci 33: 33-40.
  19. Quan LH, Liang ZQ, Kim HB, Kim SH, Kim SY, Noh YD, Yang DC. 2008. Conversion of ginsenoside Rd to compound K by crude enzymes extracted from Lactobacillus brevis LH8. J Ginseng Res 32: 226-231. https://doi.org/10.5142/JGR.2008.32.3.226
  20. Kwon SC, Choi GH, Hwang JH, Lee KH. 2010. Physicochemical property and antioxidative activity of hot-water extracts from enzyme hydroly sate of Astragalus membranaceus. J Korean Soc Food Sci Nutr 39: 406-413. https://doi.org/10.3746/jkfn.2010.39.3.406
  21. Kim KR, Jang MJ, Choi SW, Woo MH, Choi JH. 2006. Effects of water extract from enzymic-treated hamcho (Salicornia herbacea) on lipid metabolism in rats fed high cholesterol diet. J Korean Soc Food Sci Nutr 35: 55-60. https://doi.org/10.3746/jkfn.2006.35.1.055
  22. Park NY, Jeong YJ. 2006. Quality properties of oak mushroom (Lentinus edodes) based on extraction condition and enzyme treatment. J Korean Soc Food Sci Nutr 35: 1273-1279. https://doi.org/10.3746/jkfn.2006.35.9.1273
  23. Miller GL. 1959. Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal Chem 31: 426-428. https://doi.org/10.1021/ac60147a030
  24. Ando T, Tanaka O, Shibata S. 1971. Chemical studies on the oriental plant drugs. (XXV) Comparative studies on the saponins and sapogenins of ginseng and related crude drugs. Soyakugaku Zasshi 25: 28-33.
  25. Jeong JY. 2010. Enzymatic bioconversion of saponins from Panax ginseng. MS Thesis. Inje university, Gyeongnam, Korea. p 1-60.
  26. Park SY, Bae EA, Sung JH, Lee SK, Kim DH. 2001. Purification and characterization of ginsenoside $Rb_1$ metabolizing beta-glucosidase from Fusobacterium K-60, a human in testinal anaerocia bacterium. Biosci Biotechnol Biochem 64: 1163-1169.
  27. Takino Y. 1994. Studies on the pharmacodynamics of ginsenoside- Rg1, -Rb1 and -Rb2 in rats. Yakugaku Zaxxhi 114: 550-564. https://doi.org/10.1248/yakushi1947.114.8_550
  28. Jang SY, Woo SM, Kim TY, Yeo SH, Kim SB, Hong JY, Jeong YJ. 2008. Quality characteristics on enzyme treatment of brown rice (Goami ) alcohol fermentation byproduct. Korean J Food Preserv 15: 477-482.

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