• Journal of Environmental Science International
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• v.17 no.4
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• pp.439-449
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• 2008

Algicidal bacterium was isolated from sea water during the declining period of Cochlodinium polykrikoides blooms and this bacterium had a significant algicidal activity against C. polykrikoides. In this study, algicidal bacterium was identified on the basis of biochemical and chemotaxonomic characteristics, and analysis of 16S rDNA sequences. The algicidal bacterium showed 98.6% homology with Micrococcus luteus ATCC $381^T$. Therefore, this bacterium was designated Micrococcus luteus SY-13. The optimal culture conditions of the algicidal bacterium was $25^{\circ}C$, initial pH 8.0, and 3.0% NaCl concentration. M. luteus SY-13 is assumed to produce secondary metabolites which have algicidal activity. When 10% culture filtrate of this strain was applied to C. polykrikoides ($1.0\;{\times}\;10^4\;cells/ml$) cultures, over 98% of C, polykrikoides cells were destroyed within 6 hours. The culture filtrate of M. luteus SY-13 exhibited similar algicidal activity after heat-treatment at $121^{\circ}C$ for 15 min. While algicidal activity remained in filtrates with pH adjusted to 8.0, loss of algicidal activity occurred when the pHs of filtrates were adjusted to over 9.0 or heat-treated at $121{\times}180^{\circ}C$ for 1 hour. M. luteus SY-13 showed significant algicidal activities against C. polykrikoides (98.9%) and a wide algicidal range against various harmful algal bloom (HAB) species. However, there was no algicidal effect on diatom and marine livefood organisms except Isocrysis galbana. These results suggest that M. luteus SY-13 could be a candidate for use in the control of HABs.

• ALGAE
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• v.35 no.3
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• pp.225-236
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• 2020

Gymnodinium smaydae is a newly described mixotrophic dinoflagellate that feeds on only Heterocapsa spp. and Scrippsiella acuminata among 19 tested algal prey. It is one of the fastest growing dinoflagellates when feeding, but does not grow well without prey. To investigate its spatial-temporal distributions in Korean waters, we quantified its abundance in water samples that were seasonally collected from 28 stations along the Korean Peninsula from April 2015 to October 2018, using quantitative real-time polymerase chain reactions. This dinoflagellate had a wide distribution, as reflected by the detection of G. smaydae cells at 23 of the sampling stations. However, this distribution had a strong seasonality; it was detected at 21 stations in the summer and only one station in winter. The abundance of G. smaydae was significantly and positively correlated with chlorophyll a concentration as well as with water temperature. However, there were no significant correlations between the abundance of G. smaydae and salinity, concentrations of nutrients, or dissolved oxygen concentration. During the study period, G. smaydae was present when water temperatures were 7.6-28.0℃, salinities were 9.6-34.1, concentrations of NO₃ were not detectable-106.0 μM, and concentrations of PO₄ were not detectable-3.4 μM. The highest abundance of G. smaydae was 18.5 cells mL^-1 in the coastal waters of Jinhae in July 2017 when the chlorophyll a concentration was 127 mg m^-3 and water temperature was 23.8℃. Therefore, the spatial-temporal distribution of G. smaydae in Korean coastal waters may be affected by chlorophyll a concentration and water temperature.

• 한국해양학회지
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• v.26 no.1
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• pp.67-76
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• 1991

Masan Bay is infamous for its severe eutrophication, exemplified by frequent red tide incidences and anoxic conditions. We carried out daily observations for 16 days at one site immediately after the summer rainy season in 1988 on the basis that shorter observation intervals be necessary to observe a process with high turnover rate. in spite of the relatively short survey period, we could observe dramatic changes in abundance and composition of the phytoplankton populations. Cell densities and chlorophyll concentrations changed in the magnitude of 70 and 10 times, respectively, Skeletonema costatum, a diatom species, dominated the first peak of phytoplankton biomass and was followed by Prorocentrum minimum, a dinoflagellate species, which occurred dominantly in the second peak after about a week, form the viewpoint of time scale, we suggest that at least a weekly sampling might be appropriate in complex coastal environments as Masan Bay. While stratification enabled high production in the surface layer, it hindered the transport of silicate from bottom to the surface, which in turn limited the prolonged growth of diatoms. Ensued second peaks of silicate and diatom abundance in the surface layer suggest periodic flux of silicate from bottom across the discontinuity driven by tidal currents.

• 한국해양학회지
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• v.11 no.1
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• pp.25-33
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• 1976

The physical factors and chemical contents were studied at 8 stations within Masan Bay, differing in depth and location with respect to inner and outer harbours. It is a relatively small bay with a long and narrow outlet. The water temperature and salinity at the inner bay was rather subject to change on weather condition of the neighbouring land than on the effects of the water mass of outer bay. The high nutrient contents in the bay were caused by the sewage and industrial activities. The outer harbour was less polluted than that of the inner bay; the high contents were significantly decreased from the entrance of the outer harbour. While the high disolved oxygen contents, over saturation in the surface strata may be the result of nearly all year round phytoplankton blooms, the lack of oxygen contents in the bottom strata were caused by the inadequate mixing of water mass and organic matters. The frequent red tide in the area may be the results of inflowing raw sewage, industrial activities of neighbouring land and inadequate mixing of water masses.

• 한국해양학회지
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• v.26 no.4
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• pp.304-312
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• 1991

The distribution of dinoflagellate cysts have been investigated at 6 stations in Masan Bay, a well known area of red tide in the southern coastal waters of Korea, from May 1986 to March 1987. During the study, a total of 11 species in dinoflagellate cysts were isolated from surface sediments, representing 6 genera, 9 species and 2 unidentified species. The standing crops of dinoflagellate cyst varied extensively by month and station; ranging from 48 to 1,279 cells/cm$^3$ and showing major peaks in July. August and February. At stations, the distribution was most abundant at st. 4 (mouth of the bay), whereas it was very low at st. 1 (inner bay), where motile cell's blooms occur throughout the year. Thus, It is speculated that the distribution between the plankton and cyst populations of dinoflagellates show the different temporal and spatial patterns in a semi-closed bay like this survey area.

• Journal of the Korean Society of Marine Environment & Safety
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• v.16 no.4
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• pp.381-386
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• 2010

We have cultured the bluefin tuna in Yokjido, Tongyeong with floating cage which was vulnerable to harmful dinoflagellate, Cochiodinium polykrikoides. This study inspected a vertical migration of phytoplankton and Chl-a for reasonable sinking depth in floating cage. Furthermore, we analyzed the fluctuation of the phytoplankton including harmful dinoflagellates occurring in Yokgido for 6 years. Total cell density showed a significant monthly variation and the flora was predominated with diatoms. Gymnodinium sp. occurred 19 times, the greatest number of occurrence in all kinds of dinoflagellates during summer. In particular, the total number of occurrence at C. polykrikoides was 8, and harmful dinoflagellates such as Karenia brevis/Fibrcapsa japonica occurred. The relationship between Chl-a concentration/total cell number and sunset/sunrise was significant and reasonable sinking depth in floating cage was found to be at least 3m from the water surface, which was associated with massive fish kills caused by C. polykrikoides.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.27 no.6
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• pp.825-835
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• 1994

The distributions of temperature, salinity, dissolved oxygen, chemical oxygen demand, dissolved inorganic nitrogen and phosphate in the Yellow Sea are described from data collected in June and July, 1994. Based on the observations of water temperature and salinity, the fresh waters originated from the Changjiang River were found to affect the waters adjacent to Cheju Island. In the light of the distributions of dissolved oxygen and chemical oxygen demand, the western part of the Yellow Sea was worse in water quality than the eastern part. Based on data of nutrients, eutrophication indices of the western part were higher than those of the eastern part in summer. It is concluded that the western part of the Yellow Sea appeared to receive high pollution loads from rivers and was evaluated to have high potentiality of red tide occurrence.

• Journal of Environmental Science International
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• v.14 no.12
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• pp.1129-1139
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• 2005

A series of experiments was conducted to identify the potential of five phytoplankton species as standard test species for marine ecotoxicological tests. The candidate phytoplankton species are Skeletonema costatum, Heterosigma akashiwo, Prorocentrum micans, Isochrysis galbana, and Tetraselmis suecica. Salinity tolerance and sensitivity on potassium dichromate as a reference material were identified. Toxicity of eleven ocean dumped sewage sludges and four red tide expellent extracts were estimated by the inhibition of population growth rates (PGR) of marine diatom S. costatum, While most species revealed relatively weak tolerance on salinity, T. suecica demonstrated the highest salinity tolerance ranged from $5\~35$ psu and the others $15\~35$ psu. H. akashiwo revealed the highest sensitivity as 72h $IC_{50}$=0.76mg/L and T. suecica the lowest as 72h $IC_{50}$=8.89mg/L on potassium dichromate. Sludge extracts from industrial waste, domestic sewage and livestock farm waste sludge showed high toxicity as 72h $IC_{50}$<$2\%$ and lowest toxicity from filtration bed sludge as 72h $IC_{50}$=$30.50\%$ NOEC (No Observed Effective Concentration) of sludge extract ranged from <$0.4\%$ to $1.6\%$ and this indicated high phytotoxicity of ocean dumped sewage sludge. The test sensitivity of phytoplankton PGR inhibition was much higher than those of marine rotifer Brachionus plicatilis mortality test and bioluminescent inhibition test by marine bacteria Vibrio fischeri, and comparable with the sea urchin (Strongylocentrotus intermedius) fertilization test. As a result the phytotoxicity test using phytoplankton PGR inhibition ($IC_{50}$) must be a useful tool for marine phyto-toxicological evaluation of ocean dumped materials.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2003.05a
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• pp.772-777
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• 2003

선박이 배출하는 안정수(ballast water)는 외부로부터 유해 생물들이 유입되어 전파해 오는 주요경로로써 해양환경의 매우 중요하고 위험한 일종의 하나이지만, 이에 대한 효과적인 처리방법은 아직까지도 개발되지 못하였다. 그러나 최근 강 전리방전을 이용하여 고 밀집 산소와 물분자를 고농도 수산자유기(OH: hydroxyl radical)로 전리, 활성입자를 발생시켜 신속히 확산시키면 넓은 범위에서 비교적 낮은 농도로 유해성 침입 생물을 잔류물 없이 저렴한 비용으로 살균제나 촉매제의 사용 없이 소멸시켜 처리하는 효과적인 새로운 녹색방법을 제안하였다. 또한, 수산기는 강 산화제로써(산화환원 전위는 2.80 eV), 적조생물을 신속, 효과적으로 사멸시켜 잔유물과 오염물 발생 없이 이상적으로 해양적조현상을 처리할 수 있는 활성물질이다. 고출력 강 전리장치를 활용하면 수산기 활성제의 발생 농도를 Sr104 이상으로 얻을 수 있으므로, 해양적조처리에 요구되는 문턱 값 농도(~l$\times$$10^{-6}$)를 충족시킬 수 있으며, 이 경우 적조생물 소멸처리시간은 불과 10 sec 내외이므로 선박 안정수 처리문제와 함께 적조발생의 난문제를 해양동력학적으로 동시에 해결할 수 있는 효과적인 기술이다. 실험결과로부터 시간당 1 k톤의 활성물질을 발생하는 수산기활성제 제조장치의 경우, 약 4$\times$$10^2$ $\textrm{km}^2$/h의 적조해면을 처리할 수 있으며, 그 비용은 약 US$l,000 정도에 상당하므로, 적조에 따른 경제손실과는 비교될 수 없는 저렴하고 효과적인 방법이다. 활성물질의 생성시간과 가공시간은 불과 수십 $\mu\textrm{s}$ 및 수 sec 에 불과하므로, 1 kton/h 용량의 수산기활성제 제조장치의 환산소비동력은 약 200 kW이고, 장치의 체적은 10~30 ㎥의 공간으로 충분하므로, 소형선박으로 상당면적의 적조피해를 효과적으로 해결할 수 있다.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.50 no.5
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• pp.576-588
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• 2017

Some of the mariculture grounds near Ongjin-gun, Korea, were designated as mariculture management areas in 2007. Areas are so designated when the environmental quality of the mariculture ground deteriorates or there is an outbreak of hypoxia or harmful red tide that kills mariculture organisms. We surveyed the water and sediment quality and examined the mortality of mariculture organisms in the Ongjin-gun mariculture area. In a survey conducted in 2016, the water quality was better than the environmental quality standards for mariculture grounds, excepts for dissolved inorganic nitrogen, and the sediment quality was good. However, there was still mortality of mariculture organisms in some of the designated management areas. The areas that met the environmental quality standards should be delisted and the areas in which we observed mortality should be classified as management areas. This will enable the sustainable development of aquaculture and preserve healthy mariculture grounds.