• Journal of Ginseng Research
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• v.22 no.3
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• pp.188-192
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• 1998

We established the model of clonogenic assay with human tumor cell line such as Calu-3 (lung carcinoma), HEC- lB (endometrial adenocarcinoma) , HEp-2 (larnyx carcinoma), Hs-5787 (breast carcinoma), K-562 (chronic myelogenous leukemia), SF-188 (brain carcinoma), SNU-1 (stomach carcinoma) and WiDr (colon carcinoma) . We investigated growth inhibition of solvent (EtOH, MeOH) and water (100$^{\circ}C$, 121$^{\circ}C$) extracts from Korean red ginseng by clonogenic assay. The results of clonogenic assay showed that EtOH extract had growth inhibition against Calu-3, SF-188 and SNU-1, MeOH extract had growth inhibition against Calu-3, Hs-5787, K-562, and WiDr, but water extract at 100$^{\circ}C$ and water extract at 121$^{\circ}C$ had not growth inhibition against used cell lines.

• Journal of the Korean Society of Food Science and Nutrition
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• v.27 no.4
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• pp.633-638
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• 1998

Fibrionolytic activity(FA) of kimchi in rat and FAs of water and methanol extracts of kimchi ingredient were determined using fibrin plate method to see where the active principles for FA are present in kimchi. Nine Sprague-Dawley per each group were fed diet containing 3, 5 or 10% of freeze dried kimchi for 6 weeks. The FA of plasma obtained from 10% kimchi group showed stronger FA than that of control. FAs of 3 and 5% kimchi group were not noticeable. FA from methanol extract of kimchi was approximately 6 times higher than that from water extract assuming that the active material may be present in a fat soluble fraction. From water extract of kimchi ingredients, dropwort, green onion, radish and baechu showed FA in descending order. And from methanol extracts, red pepper powder, radish, green onion and baechu showed FA in decending order. The Fa of methanol extract of red pepper powder was the most noticeable among samples.

• The Korean Journal of Mycology
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• v.21 no.1
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• pp.73-76
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• 1993

227 red yeast strains were isolated from air (60 isolates), plant flowers (45 isolates), soil (40 isolates), water (37 isolates) and dairy products (45 isolates). On the basis of 33 different physiological and morphological properties, the isolated strains were assigned to 6 species belonging to 4 genera. Rhodotorula mucilaginosa and Cryptococcus albidus were the most dominant species among red yeasts of the air, plant flowers, water and dairy products, whereas Cryptococcus albidus and Rhodotorula glutinis were prevailed in soil. Cryptococcus laurentii was represented by considerable number of strains, whereas the other spesies were of low occurrence. Noteworthy was the isolation of 2 different groups of isolates belonging to Rhodotorula glutinis. These groups were differentiated from each other on the basis of rhamnose, cellobiose and arabinitol assimilation and growth at $37^{\circ}C$. Systematic position of Rhodotorula glutinis was discussed.

• Proceedings of the Ginseng society Conference
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• 1998.06a
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• pp.160-167
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• 1998

Chemical studies of nitrogen compounds of Panax ginseng seem relatively rare, Probably due to the isolation difficulties, subsequently the investigations of biological activities are little. The experimental conditions were established for highly complete extraction of peptides (basic, acidic and neutral) from Panax ginseng. This task was achieved by applying the follow isolation procedure: 1 , the sequential extraction with water, 0.1% TFA in 20% acetonitril and buffer pH 6.5 (water-pyridine-acetic acid 100:3:900) : 2, fractionation by ultrafiltration : 3, n-butanol extraction 4, cation- and anion-exchange chromatography : 5, chromato-electrophoresis. The comparison of red ginseng (xylem Sl pith part) and red ginseng inside white (xylem Sc pith part) was also provided. To analyze the peptide mixture the chromato-electrophoresis method of separation was applied. Optimal conditions for peptides mapping of sample were explored. Our experiments revealed the quantitative difference of peptide between xylem & pith and phloem & cortex part. We have also found the qualitative difference in the composition of polypeptides between normal red ginseng (xylem Sc pith part) and red ginseng inside-white (xylem St pith part)

• Journal of Ginseng Research
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• v.25 no.2
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• pp.82-88
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• 2001

This study was carried out to investigate a point of difference between normal and inferior Korean red ginseng (Naeback red ginseng = red ginseng with white part of clear boundary in phloem and/or xylem of ginseng body, saengnaeback red ginseng red ginseng with white part of indistinct boundary). White part with clear or indistinct boundary in center of ginseng body was observed in inferior red ginseng (naeback and saengnaeback red ginseng), and the differences in the internal color intensity was also found with naked eye. In hunter color values of normal and inferior parts of red ginseng in accordance with particle size, L value was increased with a diminishment in particle size, while a and b value were decreased. Absorbance at visible spectrum did not differ from water and 70% ethanol extract from normal and inferior parts of red ginseng, but absorbance in UV spectrum of extract from naeback part showed higher than those of normal and saengnaeback part. In comparison of intrastructure by electron microscope, the horizontal and vertical section of cortex and pith layer from normal part showed the very dense state, but small holes were found in naeback part of red ginseng by naked eye and electron microscope. The specific surface area of normal, naeback and saengnaeback part appeared 3.02, 3.33 and 6.55 ㎡/g, respectively. From above results, we consider saengnaeback red ginseng is red ginseng in the intermediate process which normal red ginseng changes to naeback red ginseng.

• Korean Journal of Remote Sensing
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• v.19 no.1
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• pp.21-30
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• 2003

It was studied the relationship between the red tide occurrence and the meteorological and oceanographic factors, the choice of potential area for red tide occurrence, and the satellite monitoring for red tide. From 1990 through 2001, the red tide continuously appeared and the number of red tide occurrence increased every year. Then, the red tide bloomed during the periods of July and August. An important meteorological factor governing the mechanisms of the increasing in number of red tide occurrence was heavy precipitation. Oceanographic factors of favorable marine environmental conditions for the red tide formation included warm water temperature, low salinity, high suspended solid, low phosphorus, low nitrogen. A common condition for the red tide occurrence was heavy precipitation 2∼4 days earlier, and the favorable conditions for the red tide formation were high air temperature, proper sunshine and light winds for the day in red tide occurrence. From satellite images, it was possible to monitor the spatial distributions and concentrations of red tide. It was founded the potential areas for red tide occurrence in August 2000 by CIS conception: Yeosu∼Dolsan coast, Gamak bay, Namhae coast, Marado coast, Goheung coast, Deukryang bay, respectively.

• Food Science and Preservation
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• v.25 no.1
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• pp.155-163
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• 2018

The present study was carried out to investigate the physicochemical properties of hot water extracts of red ginseng powder prepared by two-stage hot air drying method using steamed ginseng and steaming liquid for 2.5 h under high-temperature and high-pressure autoclave condition. The total polyphenols, total flavonoids, total sugar, acid polysaccharides and crude saponin in hot water extracts from red ginseng powder were analyzed and determined, and the flavor components of ginseng were measured using color difference meter and an electronic tongue. The total polyphenol, total flavonoid, total polysaccharide, and acid polysaccharide of the red ginseng hot water extract obtained by autoclaving (ARG) were 9.06 mg GAE/g, 3.38 mg NE/g, 35.22 g/100 g, and 10.90 g/100 g, respectively. The final contents of the total polyphenols, total flavonoids, crude saponin were higher than those determined using other red ginseng methods; the time required for steamed red ginseng production reduced. The total ginsenoside content of ginseng including Rb1 was 10.69 mg/g, which is the lowest ARG. The processing conditions affected the conversion to ginsenosides unique to red ginseng. Red ginseng and steaming liquid obtained from the autoclave are expected to be in need for non-food materials and products as well as foods by improving the flavor components through conversion of red ginseng components into low molecular weight.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.6 no.2
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• pp.49-62
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• 2001

Hydrographic surveys were carried out seven times during May 31, 1998 and September 24, 1998 in order to study the physical environments of the coastal area between Narodo Is. and Sorido Is. in the southern sea of Korea (the South Sea) where the occurrence of Cochlodinium polykrikoides red tide is frequently observed in summer. Temperature and salinity of the water column from the surface to the depth of 30 m exhibit large seasonal variations. Mean temperature of the water column increased by 6 and mean salinity of the water column decreased by 2.71 psu during the observation period. Both the freshwater supplied from the adjacent land and the precipitation over the study area cannot account for the observed salinity variations. The influx of the low salinity water from the offshore area is considered to be the main cause for the observed salinity changes. Surface salinity in the study area shows different spatial distribution in the period of outbreaking, mid-stage and disappearance of the red tide. Especially, salinity was abruptly lowered at the stage of disappearance of red tide as compared to salinity of the previous observation period. Vertical structure of water properties also became vertically homogeneous at the disappearance stage, while it was highly stratified in the previous observation. Such changes can only be explained by the inflow of low salinity water from the offshore, which is considered as the most possible cause for the disappearance of the red tide in the study area. This study suggests that exchanges of water, and chemical and biological factors between coastal areas and of shore area in the South Sea need to be studied in association with the general circulation of the South Sea in order for the better understanding of the occurrence and disappearance of the red tide in the coastal area of the South Sea.

• Korean Journal of Food Science and Technology
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• v.33 no.2
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• pp.184-189
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• 2001

Comparative effects of gamma irradiation and methyl bromide (MeBr) fumigation on water-soluble components of small red bean and mung bean were investigated. The levels of soluble solid and reducing sugar of soaked water at $20^{\circ}C$ for 16 h definitely increased as irradiation dose increased, which was more apparent in small red bean than in mung bean. But, their levels of MeBr-fumigated sample showed a similar result to those of the control, except for soluble solid of small red bean. The pH of soaked water was insignificantly different among treatments. The decrease in lightness (L value) and the increases of redness (a value) and yellowness (b value) were remarkable as irradiation dose increased, which was more significant in fumigated samples. The ${\Delta}E$ values of fumigated sample showed almost equaled to those of 10 kGy in red bean and 30 kGy in mung bean, respectively. Gamma irradiation caused the increase in free amino acids of soaked water, that mainly consisted of asparagine, glutamic acid, and valine, while fumigation reduced their contents. The extractables in soaked water, such as soluble solid and reducing sugar, showed a highly positive correlation with irradiation dose applied.

• Asian-Australasian Journal of Animal Sciences
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• v.17 no.5
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• pp.638-642
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• 2004

In Australia, many cropping areas are affected by salt. In these regions, Chenopodiaceous plants, such as Atriplex, Kochia and Bassia spp have been planted to improve soil conditions. These plants have become invaluable feed resources for grazing animals in dry summers, but have a high sodium content. To assess the impact of high salt intake on grazing deer, two experiments were conducted. The first experiment used 30 fallow weaner deer to examine the effect of salt level in the diet on feed intake, water intake and body weight of fallow deer. Salt was added to lucerne chaff at 0, 1.5, 3.0, 4.5 and 6% and fresh water was offered all the time. Increasing the salt level in the diet from 0 to 6% didn't affect feed intake, osmotic pressure and mineral concentration in blood of fallow deer. However, water intake was significantly higher (p<0.05) in deer fed diets containing more than 3% salt. Body weight was lower (p${\leq}$0.056) for fallow deer in July and August when salt content was over 3%, suggesting they can ingest over 15 g sodium/day without significant depression in both feed intake and growth rate if the fresh water is available. In the second experiment, 18 red weaner deer were fed lucerne chaff diets containing 1.5, 4.5 and 6.0% salt with 6 deer/diet. The results revealed that feed intake and blood osmotic pressure were similar (p>0.05) for red deer fed different levels of salt although the feed intake declined from 1.91 to 1.67 kg with the increase of salt level from 1.5% to 6.0% in the diet. Water intake was significantly higher for deer fed diets containing over 4.5% salt, but there was no difference in body weight during the experiment. However, no recommendation can be made on the salt tolerance of red deer due to limited increment of salt level in the diet.