• The Korean Journal of Blood Transfusion
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• v.29 no.3
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• pp.291-300
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• 2018

Background: The use of a functionally closed system for the glycerolization and deglycerolization of red blood cells (RBCs) allows for prolonged post-thaw storage for more than 24 hours. The aim of this study was to assess glycerolization and deglycerolization processing for RBCs using a high glycerol method in the automated, closed system provided by Haemonetics ACP 215. Methods: Thirty-five packed RBCs were glycerolized using the ACP 215 to a final concentration of 40% (wt/vol). The units were either frozen as such (n=30) or excess glycerol was removed (n=5) before freezing. After storage at $-80^{\circ}C$, the units were thawed, deglycerolized and resuspended in SAG-M. The frozen-thawed RBCs were stored at $4^{\circ}C$, and analyzed for their stability and in vitro quality. Results: No prefreeze excess glycerol removal units showed significantly less potassium leakage during post-thaw storage compared to the prefreeze excess glycerol removal units. All measurements of the stability and in vitro quality of thawed RBCs prepared from frozen RBCs without the prefreeze removal of excess glycerol during post-thaw storage at $4^{\circ}C$ for 7 days were acceptable to the American Blood Bank Association's standards and European standards. Conclusion: RBCs frozen without prefreeze removal of excess glycerol and the ACP 215 simplifies cryopreservation procedure and increases the stability of frozen-thawed RBCs. This increases the practical applicability of cryopreserved RBCs in blood transfusion practice.

• International Journal of Vascular Biomedical Engineering
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• v.1 no.2
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• pp.30-35
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• 2003

Flow characteristics of blood flow in a micro channel were investigated experimentally using a micro-PIV (Particle Image Velocimetry) velocity field measurement technique. The main objective of this study was to understand the real blood flow in micron-sized blood vessels. The Reynolds number based on the hydraulic diameter of micro-channel for deionized (DI) water was about Re=0.34. For each experimental condition, 100 instantaneous velocity fields were captured and ensemble-averaged to get the spatial distributions of mean velocity. In addition, the motion of RBC (Red Blood Cell) was visualized with a high-speed CCD camera. The captured flow images of nano-scale fluorescent tracer particles in DI water were clear and gave good velocity tracking-ability. However, there were substantial velocity variations in the central region of real blood flow in a micro-channel due to the presence of red blood cells.

• Tuberculosis and Respiratory Diseases
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• v.44 no.1
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• pp.104-113
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• 1997

Background : Toxic oxygen free radicals have been implicated as important pathologic mediators in many clinical disorders. Enhancing the intracellular content of antioxidant enzymes(superoxide dismutase, glutathione peroxidase, and catalase) can provide means of limiting biological damage caused by oxygen free radicals. The oxygen free radicals and changes of antioxidant enzymes are though to play a role in the pathogenesis of chronic obstructive pulmonary disease. Method : To investigate the pulmonary oxygen radical injury and the protective role of antioxidant enzymes in Chronic obstructive pulmonary disease(COPD), author measured the amount of thiobarbituric acid reactants, the activities of antioxidant enzymes and the sulfhydry1 groups of glutathione in serum and red blood cells from the patients with COPD(COPD patients) and the normal controls. Results : The thiobarbituric acid reactant in serum and red blood cells of COPD patients was increased than those of the normal controls, and the superoxide dismutase activity in red blood cells was no statistical difference in both groups. But the glutathione peroxidase and catalase activities in red blood cel1s of COPD patients were significantly lowered than those of the normal controls. The sulfhydry1 groups in serum and red blood cells were no statistically difference in both groups. Conclusion : These results suggest that the increased thiobarbituric acid reactants in serum and RBCs of chronic obstructive pulmonary disease mean oxygen radical toxicity, and the decreased glutathione peroxidase and catalase activities in RBC could take pan in pathogenesis of chronic obstructive pulmonary disease.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2016.05a
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• pp.715-717
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• 2016

Conventional drug carriers such as liposomes, nanoparticles, polymer micelles, polymeric conjugate and lipid microemulsion for cancer chemotherapy shield normal tissues from toxic drugs to treat cancer cells in tumors. However, inaccurate tumor targeting uncontrolled drug release from the carriers and unwanted accumulation in healthy sites can limit treatment efficacy with current conventional drug carriers with insufficient concentrations of drugs in the tumors and unexpected side effects as a result. In this research, we examined the use of sickle red blood cells as a new drug carrier with novel tumor targeting and controlled release properties. Sickle red blood cells show natural tumor preferential accumulation without any manipulation and controlled drug release is possible using a hemolysis method with photosensitizers.

• Journal of Yeungnam Medical Science
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• v.12 no.2
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• pp.339-346
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• 1995

Assays for HBsAg, HBV DNA, anti-HBc and anti-HBs of 285 units of packed red blood cells supplied by Taegu Red Cross Blood Center were performed to evaluate the correlation between the prevalence of HBV DNA and the serologic markers for hepatitis B virus. None of 285 plasma samples was positive for HBsAg, however, HBV DNA were detected by polymerase chain reaction in 2 samples which both presented only with anti-HBc positivity. Of 204 samples tested for anti-HBs, 96 samples(47.1%) were positive and among 216 samples tested for anti-HBc, 80 samples(37.0%) were positive. Of 193 samples tested for both anti-HBs and anti-HBc, 80(41.1%) were all negative and 48(24.9%) were positive on both tests. Those samples which showed positivity only to anti-HBc were 25(13.0%). Considering the above results, transfusion-transmitted hepatitis B virus infection could be prevented by discarding anti-HBc positive blood, however, that may bring insufficient supply of donor bloods in the country like Korea where the prevalence of anti-HBc is high. Anti-HBc positive blood unequivocally positive for anti-HBs should be considered noninfectious for HBV and should be allowed to be transfused. It would reduce the amount of discarding donor blood as the routine blood donor screening tests presently used at Korea Red Cross Blood Center supplemented by anti-HBs and anti-HBc testing.

• Proceedings of the KSME Conference
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• 2004.11a
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• pp.1510-1515
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• 2004

Aggregability of red blood cells (RBCs) was determined by a laser backscattering light analysis in a microfluidic channel. Available techniques for RBC aggregation often adopt a rotational Couette-flow using bob-and-cup system for disaggregating RBCs, which causes the system to be complex and expensive. A disposable microfluidic channel and vibration generating mechanism were used in the proposed new detection system for RBC aggregation. Prior to measurement, RBC aggregates in a blood sample were completely disaggregated by applying vibration-induced shear. With the present apparatus, the aggregation indexes of RBCs can be easily measured with small quantities of blood sample. The measurements with the present aggregometer were compared with those of LORCA and showed a strong correlation between them. The aggregability of the defibrinogenated blood RBCs is markedly lower than that of the normal RBCs. The noble feature of this design is the vibration-induced disaggregation mechanism, which enables to incorporate disposable element that holds the blood sample.

• Quality Improvement in Health Care
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• v.3 no.2
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• pp.70-85
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• 1997

Background : 6.1% of red blood cells and whole blood issued to the operating room was not transfused to the patients and discarded in Seoul National University Hospital in 1994. Objectives : We planned to set up an effective management program of blood in the operating room and we investigated whether this program could reduce the disposal rate of blood. Methods : We made a guideline of blood management in the operating room through a workshop. The guideline was revised after a preliminary application. The revised guideline was applied for 5 months from May to September in 1996. The disposal rate was compared before and after the installation of the new program. Results : 5,336 units of blood were issued to the operating room for 5 months. Disposal rate of red blood cells and whole blood was markedly reduced from 6.2% in May to 2.1% in September(p<0.05). The average disposal rate was 3.7% during the five months. Conclusion : We were able to reduce the disposal of unused blood in the operating room through the development and the application of a new blood management program.

• Korean Journal of Clinical Pharmacy
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• v.5 no.2
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• pp.75-84
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• 1995

Drug delivery by red cells was established to maintain the release of drugs in the blood. The entrapment method by amphotericin B was re-examined and evaluated for obtaining the suitable entrapping conditions without hemolysis. The amphotericin B treatment below $10{\mu}g/ml$ induced the non-hemolysis to entrap daunorubicin into red cells within 10min. Under these conditions intracellular ATP level was decreased as $18\%$. Membrane fluidity and the shape factor of red cells were maintained. To maintain intracellular ATP, ATP and sodium pyruvate were added during the entrapment procedure because hemolysis during the release test would reflect the loss of intracellular ATP that would be postulate the decrease of the viability invivo. Consequently, the addition of ATP in the reaction solution can raise the intracellular level of ATP.

• The Korean Journal of Physiology
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• v.5 no.2
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• pp.1-7
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• 1971

The osmotic and mechanical red cell fragility of the professional blood donors, who were found to be anemic as the result of frequent and repeated blood loss the past 5-6 years, were compared with that of the normal person while incubating the blood at $4-6^{\circ}C$ for 28 days. The fragility was expressed as % hemolysis occured during the incubation, and the following results were obtained: 1. The osmotic fragility in the normal persons (i.e, ; control group) progressively increased as the incubation became longer, and % hemolysis in 0.42% NaCl solution at 0, 10, 15, 21 and 28 incubation day was 31.90, 50.20, 41.68, 43.50 and 55.40 respectively. The mechanical fragility. in the normal red cells ranged between the minimum of 0.00% to the maximum of 5.80% both in 0.90 and 0.66% of NaCl solutions. 2. The hemolysis curve obtained in the red cell osmotic fragility from three cases of the anemic persons (i.e,; experimental group) showed a significant left side shift comparing with the normal in general which indicates that the fragility was more increased in the experimental group. The mechanical fragility in the experimental group ranged between the minimum 0.00% to the maximum 19.00% both in 0.90 and 0.66% of NaCl solutions. 3. The red cells of the chronic anemic person due to the frequent blood loss as the professional blood donor exhibit significantly marked increase both in osmotic and mechanical fragility comparing with the normal, and the tendency was more prominent as the incubation period became longer.

• Proceedings of the PSK Conference
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• 2003.10b
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• pp.125.1-125.1
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• 2003

Effects of Compound-A, a phenylpropanoid isolated from Arctium lappa fruit, on sheep red blood cells (sRBC)-induced arthus reaction (AR) were studied in ICR male mice and determined the plaque forming cells (PFC) numbers and hemagglutinin (HA) titer. Two weeks after sensitization of i.p. injection of sRBC (4x10$^{8}$ cells), ICR male mice were challenged by i.p. injection of sRBC (2$\times{10}^8$ cells). Five days after the challenge of antigen, paw edema induced three hours after the last challenge by Arthus reaction. (omitted)