• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.46 no.12
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• pp.1056-1069
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• 2018

In order to promote the commercial space activities of the private sector, the Trump Administration announced the commercial space regulatory reforms by issuing the Space Policy Directive-2 (SPD-2) on May 24, 2018, followed by the SPD-3 dealing with a separate issue of the space traffic management on June 18. Both executive orders, based on the recommendations prepared by the National Space Council (NSC) reconstituted in June 2017 and signed by the President, involve regulatory reform policy related to launch services, commercial remote sensing, establishment of one-stop shop office in Commerce Department, radio frequency spectrum, export control, and space traffic management, providing a strong guidance to the Federal Government. The commercial space regulatory reform policy can be seen in broader terms of the National Security Strategy earlier announced on Dec. 18, 2017, and as such, it pursues the economic growth of the U.S. and the national security as well. The U.S. law and policy prioritizing its national interests by promoting commercial space activities may lead to concerns and debate on the potential breach of the provisions of the Outer Space Treaty. Hence, it is worth noting the legal implications as derived from the U.S. space policy and domestic legislation, thereby accelerating international discussion to build on international norms as appropriate to the pr ogress of space technology and space commercialization.

• Korean journal of applied entomology
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• v.59 no.4
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• pp.433-441
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• 2020

This study was conducted to understand the distribution characteristics of aquatic insects in ecologically different ponds in terms of the disruption. We investigated the fauna of aquatic insects in three artificial ponds (pond 1, 2 and 3) and two natural ponds (pond 4 and 5) located within 1 km each other around Gungdaeoreum in Jeju Island, from March 2018 to June 2020. A total of 50 species belonging to 15 families were found in the surveyed ponds: total 850 individuals with 14 species in 4 families of the order Odonata, total 4,391 individuals with 14 species in 6 families of the order Hemiptera, and total 2,014 individuals with 22 species in 4 families of the order Coleoptera. In overall, total abundance and species numbers were relatively higher than those of artificial pond in natural ponds in which animal and plant ecosystems were well established. In the case of artificial ponds, the number of individuals and species recovered rapidly when reconstituted by introducing aquatic plants, etc. (Pond 1). The nymphs of Odonata were observed largely in ponds without natural enemies such as large fish, and where adults could freely access without interception by artificial structure. Phytophagous Corixidae of the order Hemiptera were abundant, and Haliplidae populations of the order Coleoptera were affected by the distribution of the plants. Accordingly, the major factors affecting aquatic insect abundance were identified as the presence of refuges such as the topography and aquatic plants and presence of predators. Species of the order, Odonata were vulnerable based on these factors. Our results can be useful as basic information for the restoration of wetlands and construction of artificial wetlands or for conservation of species diversity in the future.

• Proceedings of the KSAR Conference
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• 2001.03a
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• pp.52-52
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• 2001

Many nucleoside diphosphate (NDP) kinases are ubiquitous enzymes responsible for the exchange of ${\gamma}$-phosphates between tri- and diphosphonucleosides. The catalytic Many nucleoside diphosphate (NDP) kinases are ubiquitous enzymes responsible for the exchange of ${\gamma}$-phosphates between tri- and diphosphonucleosides. The catalytic reaction follows a ping-pong mechanism in which the enzyme is transiently phosphorylated on a histidine residue conserved in all nucleoside diphosphate kinases. Beside their role in nucleotide synthesis, these enzymes present additional functions, possibly independent of catalysis, in processes such as differentiation, cell growth, tumor progression, metastasis and development. To clone murine nm23-M5, several expressed sequence tags (ESTs) of the GenBank data base, selected according to their homology to nm23-H5 cDNA, reconstituted a complete open reading frame (GenBank AF222750). To test whether murine NDPKs (1, 2, 3, 4, 5, and 6) can inhibit Bax-mediated toxicity in yeast, co-transformation was performed respectively. The yeast S.cerevisiae was transformed with a copy expression plasmid containing the histidine selection marker and expressing murine Bax under the control of a galactose-inducible promoter. Several clones were selected and found to be growth inhibited when Bax expression was induced with galactose. A representative clone was transformed again with a copy expression plasmid containing the tryptophane selection marker and expressing either murine Bcl-xL or NDPK under the control of a galactose-inducible promoter. Several subclones of the double-transformants were selected and characterized. The ability of Bcl-xL and NDPKs to suppress Bax-mediated toxicity was determined by growing yeast cells overnight in galactose media and spot-testing on galactose plates starting with an equal number of yeast cells as determined by taking the OD$_{600}$. Ten-fold serial dilutions were used in the spot-test. Plates were grown at 3$0^{\circ}C$ for 2-3 days. All murine NDPKs suppressed Bax dependent apoptosis. Futher study will be peformed whether Bax-toxicity inhibition was caused by NDP kinase activity or additional function.n.

• Journal of the Korean Geotechnical Society
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• v.24 no.1
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• pp.91-99
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• 2008

The method to prepare the large cemented sand specimen for calibration chamber test by air-pluviation is investigated in this study. The uniformity of cemented specimen is evaluated by performing the CPTs, DMTs, and bender element tests in the calibration chamber. The sand particles, pre-wetted with 0.5% water content, are mixed with gypsum to provide the homogeneous coating of gypsum particles on the grain surface. It was shown that the pre-wetting of particle surface is effective to minimize the potential for segregation between sands and gypsum during air-pluviation. It was observed that the extreme void ratios ($e_{max}\;and\;e_{mix}$) of the mixture of pre-wetted sand and gypsum powder increase at lower gypsum content while those of the mixture of dry sand and gypsum decrease with increasing gypsum content. It was also shown from the test results that large cemented specimens reconstituted in calibration chamber by rainer system are quite uniform in vertical and horizontal directions.

• Journal of the Korean Geotechnical Society
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• v.26 no.4
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• pp.27-36
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• 2010

In this study, a series of flat dilatometer tests are performed for Busan sand reconstituted in a large calibration chamber to evaluate the state parameter ($\Psi$). Experimental result shows that the horizontal amplification factor ($K_D/K_0$) is linearly related with state parameter in semi-logarithmic space, but the $K_D/K_0$ of OC specimen is smaller than that of NC specimen because of the horizontal residual stress by stress history of OC specimen. The relation between the normalized dilatometer modulus ($E_D/\sigma_m'$) and the state parameter is also linearly expressed in semi-logarithmic space, and the effect of stress history is relatively insignificant in this relation. However, the variation in $E_D/\sigma_m'-\Psi$ relation of NC state is slightly higher than that of OC state due to the effect of the stress level, and the correlation curve is descending with increase of confining stress. The comparison of test result with previous results of Ticino and Toyoura sands shows that the $E_D/\sigma_m'-\Psi$ relation of Toyoura sand is located on upper side than that of Busan and Tieino sands due to the effect of the higher compressibility, whereas the $K_D/K_0-\Psi$ relation of each sand is irregularly distributed.

• Journal of the Korean Geotechnical Society
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• v.23 no.10
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• pp.5-11
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• 2007

This paper is to investigate the removal of the smear at elevated temperatures. This study utilized both small cylinder cell and large consolidometer apparatus to investigate the combination of PVD with heat and without heat. Two types of heaters are used in this study. The heater drain made of copper tube is used for all tests except large consolidometer and flexible wire heater is used for large consolidometer. Specimens demonstrated volume contraction upon heat and without heat. This contraction is dependent on temperature magnitude. When the specimen is heated, the magnitude of settlements is higher and rate of consolidation is faster. After treatment using PVD combined without heat and with heat for undisturbed specimens the $C_h$ values obtained were $3.45m^2/yr$ and $3.83m^2/yr$, respectively, from $3.2m^2/yr$ before treatment. The $K_h/K_s$ ratios were 3 for the specimen without heat and 2 for the specimen with heat. Similarly, in reconstituted specimens without heat and with heat, the $C_h$ values were $2.1m^2/yr$ and $2.5m^2/yr$ with $K_h/K_s$ ratios of 1.75 and 1.5, respectively.

• Asia-pacific Journal of Multimedia Services Convergent with Art, Humanities, and Sociology
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• v.6 no.10
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• pp.449-456
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• 2016

In this study we recomposed the life story of Min Tatyana who is a Koryo-saram, or in other words, an ethnic Korean residing Kazakhstan. The life story of an overseas Korean, or Koryo-saram to be exact, includes in itself their multi-level identity which consists of the period of the immigration of Koryo-sarams, the special incident of deportation, as well as life as people of the Soviet Union and Kazakhstan, and life as a Korean race. In this study, we were confirmed the texture of the Korean race thought ordinary korean Min Tatyana life who living in Kazakhstan. The life story of Min Tatyana was reconstituted into two stories as "growing up in a multicultural society as Koryo-Saram" and "to live a life as the descendants of independence activist". She was born in historically region Kyzylorda, where living Korean groups and now she is living in Almaty, where living the many koreans ethnic groups of Kazakhstan. Also, her parents were respected to the local residents and her grandfather of husband was famous independent activist. These aspects have helped keep her Korean ethnic identity. The life story of Min Tatyana is personal story and qualitative text, which shows social, cultural background of korean ethnic who living in Kazakhstan. This study is expected to help to confirm the Koryo-Saram characteristics and aspect of their multilayer life.

• Analytical Science and Technology
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• v.20 no.4
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• pp.347-354
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• 2007

A simple and rapid high-performance liquid chromatography assay for the determination of residual novobiocin levels in bovine, porcine, chicken, flatfish and japanese eel muscle has been developed and validated. The separation condition for HPLC/UV was optimized with phenyl hexyl ($4.6{\times}150mm$, $5{\mu}m$) column with 10 mM monobasic sodium phosphate buffer (pH 2.5)/acetonitrile (50/50, v/v) as the mobile phase at a flow rate of 1.0 mL/min and detection wavelength was set at 254 nm. Residues were extracted from tissue by blending with methanol and lipid materials were removed with n-hexane. Then, the methanol extract was evaporated to dryness under a nitrogen stream, reconstituted in the mobile phase. Aliquot of the organic extract was decanted and filtered through $0.45{\mu}m$ syringe filter. The $20{\mu}L$ of the resulting solution was injected into the HPLC system. The calibration ranges were $0.5{\sim}5{\mu}g/g$ and calibration curves were linear with coefficients of correlation better than 0.95. The limits of quantification were $0.5{\mu}g/g$ for all muscles. The recoveries of bovine, porcine, chicken, flatfish and japaneseel muscles were 99.8%, 102.4%, 91.0%, 104.0% and 93.0%, respectively. The procedures were validated according to the CODEX guideline, determining specificity, linearity, accuracy, precision, quantitation limit and recovery.

• Korean Journal of Veterinary Research
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• v.33 no.1
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• pp.151-169
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• 1993

The present study was carried out to investigate the best condition for nuclear-cytoplasm fusion and in vitro culture of nuclear transplanted embryos and to investigate the production of nuclear transplanted offsprings. The nuclei from 2-, 4- and 8-cell mouse embryos were transferred into enucleated 2-cell embryos, and the reconstituted embryos were submitted to direct current(DC) pulses at output voltage of 1.0, 1.5 and 2.0 kV/cm for 100, 150 and $200{\mu}$ sec to induce cell fusion. 1. The culture of intact or zona cut 2-cell embryos in the medium supplemented with cytochalasin B($5{\mu}g/m{\ell}$) and colcemide($0.1{\mu}g/m{\ell}$)for 30 and 60 minutes did not affect the development to later stage. 2. The in vitro developmental rates of group A(a nucleus from one of the blastomeres was removed) and B(electrofusion of group A) were significantly lower than that of control group(p<0.01). 3. When nuclear transplanted embryos were submitted to electrofusion, the significantly higher fusion rates of 2-cell donor nuclei were achieved at the electric field strength of DC 1.5kV/cm for 100 and $150{\mu}$ sec, DC 2.0 kV/cm for $100{\sim}200{\mu}$ sec than DC 1.0 kV/cm for 100 and $150{\mu}$ sec(p<0.01). The significantly higher fusion rates of 4-cell donor nuclei were achieved at DC 2.0 kV/cm for 100 and $150{\mu}$ sec than DC 1.0kV/cm for $100{\sim}200{\mu}$ sec(p<0.01). These fusion rates in 8-cell donor nuclei were 88.7~99.3%. 4. The developmental potency to blastocyst in 2- and 4-cell donor nuclei was significantly higher in DC 1.0 and 2.0 kV/cm for $100{\sim}200{\mu}$ sec treated group and DC 2.0 kV/cm for 150 and $200{\mu}$ sec treated group (p<0.01). The developmental potency to blastocyst in 8-cell donor nuclei was significantly higher in DC 2.0 kV/cm for $100{\mu}$ sec treated group than in DC 1.0 kV/cm for $100{\mu}$ sec treated group and DC 2.0 kV/cm for 150 and $200{\mu}$ sec treated group(p<001). 5. The developmental potency to blastocyst after nuclear transplantation was significantly higher in 2-cell donor nuclei than in 8-cell donor nuclei(p<0.01). 6. The success rate of nuclear injection into enucleated 2-cell embryos was significantly higher in 2-cell donor nuclei than in 4- or 8-cell donor nuclei(p<0.01). 7. The culture time taken for the nuclear transplanted 2-cell embryos to blastocyst stage was significantly longer in 2-cell donor nuclei than in 8-cell donor nuclei(p<0.01). 8. There was no significant difference in the developmental potency of nuclear transplanted embryos within the concentration of EGF at 0 to 15 ng per $m{\ell}$ of BMOC-3 solution. 9. The production rates of offspring after transfer of nuclear transplanted embryos to recipient mouse were significantly higher in 2-cell donor nuclei than in 8-cell donor nuclei(p<0.01).

• The Korean Journal of Pesticide Science
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• v.16 no.3
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• pp.261-266
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• 2012

It is common to use many experiment animals to evaluate the toxicity of chemicals including pesticides. For protecting animal, the concepts of 3R (Reduction, Replacement, Refinement) were introduced and in vitro alternatives methods actively have been developed all over the world. Many experimental animals for toxicological tests have been used, so that it is important to establish the alternative methods. In this study, the alternative method using reconstituted human skin model (Keraskin$^{TM}$) was conducted for classification of skin irritation on pesticides. Sixteen formulations selected on the basis of the degree of irritation were treated by Keraskin$^{TM}$ test. The percent of cell viability was measured into the culture medium collected after treatment of the pesticides for 24-72 hrs. The skin irritations of formulations were evaluated by the cell viability. In this study, The 4 formulations with mild irritation in rabbits were evaluated as nonirritant, the 6 formulations with moderate and severe irritation were evaluated as irritant in human skin model test. We suggest that the alternative test using Keraskin$^{TM}$ model could be used as toxicity evaluation for primary irritation index (P.I.I.) score of greater than or equal to 2.1 of pesticides. The further studies should be required to apply for hazardous assessment of pesticides on alternative skin irritation methods because of the interindividual variability of the sensitivity of skin irritation on pesticides.