• Journal of Aquaculture
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• v.21 no.4
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• pp.309-316
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• 2008

Effects of the various dietary additives on growth and tolerance of abalone Haliotis discus hannai to the stresses were determined in the 16-week feeding trial. Seventy juvenile (an initial body weight of 4.2 g) abalone per container were randomly distributed into 21, 50 L plastic rectangular containers each. The six kinds of experimental diets were prepared: control (CON) with no additive, by-product of green tea (BPG), extract of figs (EF), extract of green tea (EG), commercially available product of Hearok (PH), and Haematococcus (HC). In addition, dry sea tangle (ST) was prepared to compare the efficiency of the experimental diets. Fishmeal, soybean meal and shrimp head meal were used as the protein source, and dextrin, sea tangle powder and wheat flour, and soybean oil and fish oil were used as the carbohydrate and lipid sources, respectively in the experimental diets. The experimental diets were fed to abalone once a day at a satiation level with a little leftover. The feeding trial lasted for 16 weeks. At the end of the 16-week feeding trial, abalone was exposed to the different types of stresses (air exposure, and sudden changes of rearing temperature and salinity). Survival of abalone fed the sea tangle was highest. However, weight gain of abalone fed the EF, EG and PH diets was significantly (P<0.05) higher than that of abalone fed the BPG diet or dry sea tangle. Shell length of abalone fed the all experimental diets was significantly (P<0.05) higher than that of abalone fed the dry sea tangle. Accumulated mortality of abalone fed the sea tangle was low when exposed to the different types of stresses. Also, relatively low mortality was achieved in abalone fed the HC and EF diets. In considering these results, it can be concluded that the various sources of additives is effective to improve production of abalone, and Haematococcus and extract of figs can be considered as dietary additives to improve resistance of abalone against the different types of stresses.

• Journal of Sericultural and Entomological Science
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• v.52 no.2
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• pp.110-116
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• 2014

Recently the number of the butterflies, Mellicta ambigua, had been decreasing rapidly, and already disappeared at many habitat. In this studies, we investigated ecological environment of Mellicta ambigua for preparing of primary research data recovering habitat, and studied on bionomical characteristics. Two different habitat, Jindo and Inje, were selected for investigation of ecological environment. We investigated four times during 3-month, from June to August in 2012. In Jindo, we observed more than 100 butterflies and a lot of host plants, Melampyrum roseum var. japonicum. But only 5 butterflies and only a few host plants, Veronicastrum sibiricum were observed in Inje. We could not observe the eggs, the larva and pupa on the host plants at all. For finding of bionomical characteritics, we reared butterflies at natural conditions. Collected 3-female butterflies from Jindo laid 465 eggs on the leaves of 3-host plants, Veronicastrum sibiricum. 120 ~ 186 eggs per each female were laid in the shape of cluster. An egg was globular shape, 0.6 mm diameter and 0.7 mm height. The egg periods were $9.96{\pm}0.4days$ after ovipositioning, and the hatchability was 95.% at natural condition. The larval periods were $4.1{\pm}0.6days$ (1st instar), $2.1{\pm}1.0days$ (2nd), $8.1{\pm}0.7days$ (3rd), $239.2{\pm}10.9days$ (4th), $12.3{\pm}1.3days$ (5th), $17.1{\pm}1.1days$ (6th), $10.5{\pm}1.0days$ (7th) each other. The larva of 4th instar overwintered in the nest that had been made into the leaf of host plant with secreted thread as a group until early March next year. In the early March next year, overwintered larva went around their nest in search of host plants, and went to other host plants, Veronica persica and Plantago asiatica, sometimes. The overwintered larva of Mellicta ambigua could grow up on two other host plants normally. In the following experiment, the butterflies of Mellicta ambigua laid eggs on the leaves of Plantago asiatica, but the 1st instar larva from eggs died all. The headwidth of each developmental larval stage were $0.28{\pm}0.02mm$ (1st), $0.45{\pm}0.02mm$ (2nd), $0.58{\pm}0.02mm$ (3rd), $0.75{\pm}0.03mm$ (4th), $0.89{\pm}0.05mm$ (5th), $1.23{\pm}0.06mm$ (6th), $2.13{\pm}0.11mm$ (7th). The pupal ratio was 92.0%. The pupal period were $9.1{\pm}1.6days$, and the emergence rate was 88.6%. As a result we determined that Mellicta ambigua can rear at natural conditions. But indoor-rearing is considered to be difficult and not useful industrially, because they have long term larval stage and only one life cycle per an year.

• Korean Journal of Ichthyology
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• v.27 no.1
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• pp.21-25
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• 2015

We studied the effects of temperature and salinity on the egg development and larvae of sevenband grouper Epinephelus septemfasciatus under sea cage culturing condition. In regard to rearing environment, the water temperature is $21.5{\sim}23.5^{\circ}C$ (mean $22.0{\pm}0.05^{\circ}C$) and the salinity is 32.0~33.0 psu (mean $32.5{\pm}0.05psu$). The time of egg development was positively proportional to water temperature with 29 hrs, 27 hrs, 24 hrs, 17 hrs, 15 hrs after fertilization in $15^{\circ}C$, $20^{\circ}C$, $25^{\circ}C$, $30^{\circ}C$, $35^{\circ}C$ respectively. Each of the salinity hatching rate was 80% in the section of 15 psu, 81.8% in the section of 25 psu, 89.1% in the section of 30 psu, and 79.1% in the section of 35 psu. All things considered, the highest hatching rate appeared in the range of the section of 25~30 psu. Abnormalities of low salinity section, such as 15 psu (20.0%) and 20 psu (18.2%), was lower than the abnormalities of 35 psu that belongs to high salt section. The relation between the time of egg development (t:hour) and water temperature ($T:^{\circ}C$) was represented by the mathematical formulae. The mean biological minimum temperature was $5.4^{\circ}C$.

• Korean Journal of Childcare and Education
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• v.6 no.2
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• pp.155-173
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• 2010

The purpose of this study was to find factors affecting the psychological well-being of married working women in Seoul. For this purpose, survey data form 332 employed married women residing in Seoul are used to evaluate the relative effects of Psychological well-being of married working women. Survey questions included demographic information, housework and child-rearing related variables, work related variables, variables related with social support systems, items on child care services for the preschoolers, and psychological well-being of married working women. The major findings were as follows: 1) The psychological well-being of married working women score is 36.14(mean score is 32). The difference between the psychological well-being of married working women varied this according to socio-demographic variables: educational level, income level, spouse's support, occupation, and job satisfaction. 2) The factors that affected psychological well-bing were amount of household labor by husband, job satisfaction, socio-support systems, and educare service satisfaction. 3) The factor that had the most significant impact on the psychological well-being of married working women is job satisfaction. It is suggested to utilize the Employment Assistant Program to establish friendly working environment for married women.

• Korean Journal of Agricultural Science
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• v.30 no.1
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• pp.11-19
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• 2003

The life-cycle of the aphid-parasite, Aphidius gifuensis, took 11.9 days at $25^{\circ}C$ and 12.1 days at $30^{\circ}C$. The female appearance rate of A. gifuensis was the best at $20{\sim}25^{\circ}C$ compared to the other temperatures. Optimum rearing temperature of Aphidius gifuensis was $20{\sim}25^{\circ}C$ Optimum temperatures for A. gifuensis emergence were $20^{\circ}C$ and $25^{\circ}C$ with 99.1% and 98.2%, respectively. However the emergence rate of Aphidius gifuensis was 58.8% at $10^{\circ}C$. There was a positive correlation between A. gifuensis adult activity and temperature, but there was a negative correlation between developmental period of Myzus persicae nymph and temperature. Among the pests occurring in pepper greenhouse, dominant species was M. persicae and its density was 6~1,024 per plant. The damaged fruit rate by Helicoverpa assulta was 3.3~53.3%. The number of aphid and mummy in the A. gifuensis released plot were 173.5 and 10 in June, 1.8 and 17 in July, 2000, respectively. The numbers of Aphidius gifuensis were 7.5 and 0.4 in May, 27.1 and 2.1 in June, 2001, respectively. The suppressive effects on M. persicae in A. gifuensis released plot was better than the control plot, but there was no significant difference compared to the pesticide-sprayed plot. The first leaf length and the stem width in the A. gifuensis released plot, the control plot, and the pesticide-sprayed plot were not significantly different, but the A. gifuensis released plot showed the highest yield among the plots.

• Journal of Aquaculture
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• v.18 no.1
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• pp.37-44
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• 2005

This study was conducted to compare the physiological conditions of black porgy (Acanthopagrus schlegeli) when reared in freshwater (0 psu) and seawater (35 psu) during 90 days. In terms of stress response, there was no significant difference in cortisol levels of the fish reared either freshwater or seawater until 60 days. Although cortisol level of fish reared in freshwater(12.6$\pm$5.0 ng/ml) was significantly higher than in seawater (4.5$\pm$2.9 ng/ml) at the end of experiment, these values were stable levels as compared with that of non-stressed fish. No significant differences in plasma osmolalities were recognized between the fish reared in freshwater (346.7$\pm$4.6$\~$356.5$\pm$2.1 mOsm/kg) and seawater (350.0$\pm$2.0$\~$357.0$\pm$22.6 mOsm/kg). Normal structure of gill lamellae without histological damage or cell necrosis has been observed in the fish reared in freshwater. In connection with growth and survival rate, total length and body weight of fish reared in freshwater were slightly longer and higher than those of fish reared in seawater. Survival rate of black porgy reared in freshwater was slightly higher than that of fish reared in seawater, but there was no significant difference. The results suggest that black porgy is reared with normal growth rate in freshwater without stress.

• Korean Journal of Ecology and Environment
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• v.45 no.3
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• pp.314-321
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• 2012

To quantitatively assess the effects of turbidity on egg development and larval fish survival, a laboratory fish rearing experiment was applied to different life stages (newly hatched larval stage, juvenile stage and pre-adult stage) of the Zacco platypus, one of the most universal and tolerant species in Korea. According to the stress index of turbidity in water with exposure time, three different treatments, including a reference condition (1~7 NTU) as well as intermediate (20~150 NTU) and high turbidity conditions (400~1,000 NTU) were applied, and egg hatching and larval fish mortality rates were observed. The mortality rates of newly hatched larval fish were significantly different among treatments (ANOVA, $F_{2,3}$=17.79, p<0.05). Average rates of survival to hatching were 20.9% (${\pm}0.1%$) for reference condition, 11% (${\pm}6.9%$) for intermediate level and 3.2% (${\pm}3.7%$) for high level conditions, respectively. A sudden change of mortality at the high level was observed within 5 days of the experiment. About 84% of juvenile fish survived until 20 experimental periods under conditions of reference turbidity, while survival under conditions of intermediate turbidity was over 80% of larval fish until day 13 of the experiment, but dropped to less than 10% after day 14 and 15 of two experiments. Fish mortality appeared from day 6 of the high turbidity experiment, and 50% mortality was achieved at day 9 to 10 of experiment. Full mortality occurred at day 14 of the experiment (RM-ANOVA, $F_{2,38}$, p<0.005). In the pre-adult stage experiment, no mortality was observed during the experiment at reference level treatment (20 days), while only slight mortality rates were observed for both intermediate and high levels until day 5 of the experiment, however, no further fish died in either experiment. It was significantly different compared to reference condition (RM-ANOVA, $F_{2,20}$=8.28, p<0.01), but no difference was observed between intermediate and high level conditions. Consequently, this tolerant species has been determined to be well adapted to high levels of turbidity in its adult stage, but more vulnerable throughout earlier life stages.

• Korean Journal of Ecology and Environment
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• v.43 no.3
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• pp.409-417
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• 2010

Present study aimed to investigate morphological and physiological change in the tissues of Carassius auratus after exposure against Pb (lead) with various rearing condition. We measured the level of accumulated heavy metal and analyzed ultrastructure with transmission electron microscopy. The heavy metal, Pb, was accumulated in the gill, bone and integument increased drastically for exposure periods, the 40 days. The accumulation of Pb in the gill showed relatively higher than that in other tissues. And accumulated Pb amounts also were increased with exposure time dependent manner in the gill, bone and integument tissues. Also, specific activities of antioxidation enzymes in all tissue after exposure to Pb were increased in the course of exposure. And the activities of SOD from tissues exposed to Pb were increased 2 folds than those from the unexposed but GPX activities were maintained constant. The increased numbers of mucous cells in gill tissues exposed to Pb were determined and morphological changes, such as clubbing and fusion, were shown secondary lamella. Also, exposure of Pb for 40 days on gills tissues cause membrane damage in mitochondria and nucleus. In kidney tissues, the atrophied glomerulus was observed, and the empty space in Bowman's capsule was expanded. Based on the all results, it is suggested that the exposure to the high level of Pb for long period affect on the morphology of tissues, and change the enzymatic balance in C. auratus.

• International Journal of Industrial Entomology and Biomaterials
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• v.18 no.2
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• pp.105-112
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• 2009

The greenhouse whitefly, Trialeurodes vaporariorum (Westwood) and the sweet potato whitefly, Bemisia tabaci (Gennadius) are serious insect pests that have a wide host range including cucumber, tomato, and pepper. In this study, we tested larvicidal efficacy of several on-the-market environment-friendly agricultural materials (EFAM) to select the effective products after the target pests were stabilized in indoor rearing condition. The developmental periods of two whiteflies are as follows: in the case of T. vaporariorum, egg duration is 9.6 days and nymph is 18.9 days, and in the case of B. tabaci, egg duration is 7.4 days and nymph is 15.2 days under $25^{\circ}C$ with relative humidity (RH) of $60{\pm}5%$ and a photoperiod of 16 L:8D. The total period of T. vaporariorum was 5 days longer than B. tabaci. Among 22 EFAMs six products showed more than 60% of insecticide efficacy against T. vaporariorum BTV B, BTV D, BTV G, BTV L, BTV M, and BTV S. On the other hand, seven EFAM products showed over 60% of insecticide efficacy against B. tabaci BTV D, BTV G, BTV K, BTV L, BTV M, BTV N, and BTV U. In the case of Spodptera litura previously, 16 EFAMs were tested against $2^{nd}$ instar S. litura, and six EFAMs were found to have more than 90% efficacy. Test of these six EFAMs against entire larval stages were performed in this study. Although some of these products showed still more than 90% of insecticidal efficacy against up to $3^{rd}$ instar larvae, the efficacy of these EFAMs sharply decreased as ages increase, resulting in less than 60% of efficacy of the products at most. This result indicates the difficulty to control S. litura with the on-the-market EFAMs alone under economic injury level. Collectively, it is required to find more EFAMs, and find alternative method, and combined way of controlling to control those insect pests tested in this study.

• Journal of Sericultural and Entomological Science
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• v.51 no.1
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• pp.20-29
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• 2013

Countries of the world are competing for the collection and utilization of genetic resources, which is a resource of value-added industrialization. We are building database of silkworm and mulberry resources with morphological, genetic characteristics data etc. Moreover, recent bioactive compound has been receiving increasing attention. So we analyzed 1-deoxynojirimycin(1-DNJ) content as a this item in addition to the basic information using 363 strains(varieties) of mulberry genetic resources. They were grown under the same environment and conditions. Mulberry leaves were collected and then freeze-dried and powdered for 1-DNJ test. As a result, 1-DNJ mean content of 363 mulberry strains was $0.176{\pm}0.077%$, and the coefficient of variation (CV) was 43.5%. The variation between the strains was greatly severe. Among the tested strains, 'C1D89/29' was showed the highest content of 0.47%, whereas 'Pumbo 24' and 'Turkey E' were showed the lowest content of 0.05% respectively. The content of 1-DNJ of 16 mulberry varieties for silkworm rearing were compared. Average concentration was $0.17{\pm}0.04%$, and the coefficient of variation (CV) was 22.8%. Variation among cultivars was not significant. Finally we selected 1-DNJ high-containing 11 strains. They are as follows. 'C1D89/29', 'Hiihak', 'Jeokasibmunja', 'Gweonchil', 'Botongsibmunja', 'Jeokchuk', 'bulguksang', 'Geunsookgojo', 'Busanggeum', 'Guksang 20' and 'Taekwang' which are more than twice of the overall average content.