• Molecules and Cells
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• v.42 no.4
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• pp.321-332
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• 2019

The brain is the most common metastatic site of lung adenocarcinoma; however, the mechanism of this selective metastasis remains unclear. We aimed to verify the hypothesis that exposure of tumor cells to the brain microenvironment leads to changes in their gene expression, which promotes their oriented transfer to the brain. A549 and H1299 lung adenocarcinoma cells were exposed to human astrocyte-conditioned medium to simulate the brain microenvironment. Microarray analysis was used to identify differentially expressed genes, which were confirmed by quantitative real-time PCR and western blotting. Knockdown experiments using microRNAs and the overexpression of genes by cell transfection were performed in addition to migration and invasion assays. In vitro findings were confirmed in clinical specimens using immunohistochemistry. We found and confirmed a significant increase in insulin-like growth factor binding protein-3 (IGFBP3) levels. Our results also showed that the up-regulation of IGFBP3 promoted A549 cell epithelial-mesenchymal transition, migration, and invasion, while the knockdown of IGFBP3 resulted in decreased cell motility. We also found that Transforming growth factor-${\beta}$ (TGF-${\beta}$)/Mothers against decapentaplegic homolog 4 (Smad4)-induced epithelial-mesenchymal transition was likely IGFBP3-dependent in A549 cells. Finally, expression of IGFBP3 was significantly elevated in pulmonary cancer tissues and intracranial metastatic tissues. Our data indicate that up-regulation of IGFBP3 might mediate brain metastasis in lung adenocarcinoma, which makes it a potential therapeutic target.

• Journal of Digital Convergence
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• v.17 no.1
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• pp.195-201
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• 2019

This paper proposes a blockchain system to share Wireless Local Area Network (WLAN) that recently suffers from mutual interference among increasing devices using unlicensed bands. Blockchain technology can induce cooperation from users by incentivizing them with cryptocurrency like shown in Bitcoin example. In this paper, we describe Blockchain based access mechanism in WLAN instead of conventional authentication based access. Here, users can access any WLAN access point by paying through smart contract while they also receive payment from others. In order to support real-time transaction, we apply proof-of-authority that is realized by Byzantine fault tolerant protocol instead of well-known proof-of-work that requires huge computing power and delay.

• Journal of the Korea Institute of Information Security & Cryptology
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• v.22 no.3
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• pp.447-461
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• 2012

A social network service(SNS) is gaining popularity as a new real-time information sharing mechanism. However, the user's privacy infringement is occurred frequently because the information that is shared through a social network include the private information such as user's identity or lifestyle patterns. To resolve this problem, the research about privacy preserving data sharing in social network are being proceed actively. In this paper, we proposed the efficient scheme for privacy preserving data sharing in social network. The proposed scheme provides an efficient conjunctive keyword search functionality. And, users who granted access right to storage server can store and search data in storage server. Also,, our scheme provide join/revocation functionality suited to the characteristics of a dynamic social network.

• Journal of the Korea Institute of Information Security & Cryptology
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• v.22 no.6
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• pp.1293-1300
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• 2012

With the rapid evolution of mobile devices and the development of wireless networks, users of mobile social network service on smartphone have been increasing. Also the security of personal information as a result of real-time communication and information-sharing are becoming a serious social issue. In this paper, a framework that can be linked with a social network services platform is designed using OpenAPI. In addition, we propose an authentication and detection mechanism to enhance the level of personal information security. The authentication scheme is based on an user ID and password, while the detection scheme analyzes user-designated input patterns to verify in advance whether personal information protection guidelines are met, enhancing the level of personal information security in a social network service environment. The effectiveness and validity of this study were confirmed through performance evaluations at the end.

• Journal of IKEEE
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• v.25 no.2
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• pp.291-301
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• 2021

OPC UA at the control and field level does not provide enough performance to replace the field bus. The OPC Foundation aims for a real-time and connection-less mechanism, and has added the OPC UA publish-subscribe model, a new specification that supports broker functions such as MQTT and AMQP, as the OPC UA Part 14 standard. This paper is about a gateway for interoperability between OPC UA publisher with the addition of OPC UA Part14 standard and DDS subscribers. Raspberry Pi 4 is used for the gateway proposed in this paper, and OpenDDS, an open source, is used for DDS. OPC UA publish-subscribe module used A-Open62541 publish-subscribe module, which additionally implements functions not provided by the corresponding source based on Open62541 publish-subscribe open source.

• The Journal of Korean Institute of Information Technology
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• v.17 no.5
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• pp.105-116
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• 2019

Most of the public and private buildings in Korea are installing CCTV for crime prevention and follow-up action, insider security, facility safety, and fire prevention, and the number of installations is increasing each year. In the questionnaire conducted on the increasing CCTV, many reactions were positive in terms of the prevention of crime that could occur due to the installation, rather than negative views such as privacy violation caused by CCTV shooting. However, CCTV poses a lot of privacy risks, and when the image data is collected using the cloud, the personal information of the subject can be leaked. InseCam relayed the CCTV surveillance video of each country in real time, including the front camera of the notebook computer, which caused a big issue. In this paper, we introduce a system to prevent leakage of private information and enhance the security of the cloud system by processing the privacy technique on image information about a subject photographed through CCTV.

• Animal Bioscience
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• v.35 no.1
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• pp.22-28
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• 2022

Objective: Endoplasmic reticulum (ER) stress engages the unfolded protein response (UPR) that serves as an important mechanism for modulating hepatic fatty acid oxidation and lipogenesis. Chronic fasting in mice induced the UPR activation to regulate lipid metabolism. However, there is no direct evidence of whether negative energy balance (NEB) induces ER stress in the liver of cows. This study aimed to elucidate the relationship between the NEB attributed to feed deprivation and ER stress in bovine hepatocytes. Methods: Blood samples and liver biopsy tissues were collected from 6 non-lactating cows before and after their starvation for 48 h. The blood non-esterified fatty acids (NEFA), β-hydroxybutyric acid (BHBA) and glucose level were analyzed. Real-time quantitative polymerase chain reaction and Western blotting were used to explore the regulation of genes associated with UPR and lipid metabolism. Results: The starvation increased the plasma BHBA and NEFA levels and decreased the glucose level. Additionally, the starvation caused significant increases in the mRNA expression level of spliced X-box binding protein 1 (XBP1s) and the protein level of phosphorylated inositol-requiring kinase 1 alpha (p-IRE1α; an upstream protein of XBP1) in the liver. The mRNA expression levels of peroxisome proliferator-activated receptor alpha and its target fatty acid oxidation- and ketogenesis-related genes were significantly upregulated by the starvation-mediated NEB. Furthermore, we found that the mRNA expression levels of lipogenic genes were not significantly changed after starvation. Conclusion: These findings suggest that in the initial stage of NEB in dairy cows, the liver coordinates an adaptive response by activating the IRE1 arm of the UPR to enhance ketogenesis, thereby avoiding a fatty liver status.

• Tunnel and Underground Space
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• v.31 no.6
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• pp.385-399
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• 2021

Among human-induced seismicity, fluid production has been one of the causes. In this report, the mechanism that causes an earthquake due to a decrease in the fluid pressure inside the reservoir during fluid extraction is summarized. As case studies, the Lacq gas field in France, the Cerro Prieto geothermal field in Mexico, and the Groningen gas field in the Netherlands, which have become issue recently, were introduced. It is showed that fluid production, ground subsidence, and the presence of existing faults were closely related with the induced seismicity. Therefore, for the development of oil or gas field and geothermal field, it is important to investigate the presence of faults that may cause earthquakes in the reservoir, to monitor ground subsidence during production in real time, and to control production.

• Journal of Veterinary Science
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• v.23 no.4
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• pp.56.1-56.17
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• 2022

Background: At the therapeutic doses, diclofenac sodium (DFS) has few toxic side effects on mammals. On the other hand, DFS exhibits potent toxicity against birds and the mechanisms remain ambiguous. Objectives: This paper was designed to probe the toxicity of DFS exposure on the hepatic proteome of broiler chickens. Methods: Twenty 30-day-old broiler chickens were randomized evenly into two groups (n = 10). DFS was administered orally at 10mg/kg body weight in group A, while the chickens in group B were perfused with saline as a control. Histopathological observations, serum biochemical examinations, and quantitative real-time polymerase chain reaction were performed to assess the liver injury induced by DFS. Proteomics analysis of the liver samples was conducted using isobaric tags for relative and absolute quantification (iTRAQ) technology. Results: Ultimately, 201 differentially expressed proteins (DEPs) were obtained, of which 47 were up regulated, and 154 were down regulated. The Gene Ontology classification and Kyoto Encyclopedia of Genes and Genomes pathway analysis were conducted to screen target DEPs associated with DFS hepatotoxicity. The regulatory relationships between DEPs and signaling pathways were embodied via a protein-protein interaction network. The results showed that the DEPs enriched in multiple pathways, which might be related to the hepatotoxicity of DFS, were "protein processing in endoplasmic reticulum," "retinol metabolism," and "glycine, serine, and threonine metabolism." Conclusions: The hepatotoxicity of DFS on broiler chickens might be achieved by inducing the apoptosis of hepatocytes and affecting the metabolism of retinol and purine. The present study could provide molecular insights into the hepatotoxicity of DFS on broiler chickens.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.16 no.9
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• pp.3138-3151
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• 2022

Traffic safety and congestion are becoming more and more serious, especially the frequent occurrence of traffic accidents, which have caused great casualties and economic losses. Cellular Vehicle to Everything (C-V2X) can assist in safe driving and improve traffic efficiency through real-time information sharing and communication between vehicles. All vehicles communicate directly with Base Stations (BS), which will increase the base station load. And when the communicating vehicles are too far apart, too fast or there are obstacles in the communication path, the communication link can be unstable or even interrupted. Therefore, choosing an effective and reliable multi-hop relay-assisted Vehicle to Vehicle (V2V) communication can not only reduce the base station load and improve the system throughput but also expand the base station coverage and improve the communication quality of edge vehicles. Therefore, a communication area division scheme based on regular hexagon segmentation technology is proposed, a relay-assisted V2V communication mechanism is designed for the divided communication areas, and an efficient communication link is constructed by selecting the best relay node. Simulation results show that the scheme can improve the throughput of the system by nearly 55% and enhance the robustness of the V2V communication link.