• Journal of Advanced Navigation Technology
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• v.17 no.6
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• pp.736-748
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• 2013

In this paper, we analyse the vulnerabilities of KL scheme which is an ID-based authentication scheme for AMI network, and propose two kinds of authentication schemes which satisfy forward secrecy as well as security requirements introduced in the previous works. In the first scheme, we use MDMS which is the supervising system located in an electrical company for a time-synchronizing server, in order to synchronize smart grid devices in home, and we process device authentication with a new secret value generated by OTP function every session. In the second scheme, we use a secret hash-chain mechanism for authentication process, so we can use a new secret value every session. The proposed two schemes have strong points and weak points respectively and those depend on the services area and its environment, so we can select one of them efficiently considering real aspects of AMI environment.

• Journal of KIISE:Software and Applications
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• v.26 no.11
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• pp.1298-1305
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• 1999

네트워크 및 분산 처리 기술의 발달로 분산 환경에서 동작하는 응용 프로그램에 대한 생산성 향상이 요구되고 있다. 본 논문에서는 MVC(Model, View, Controller) 패턴을 기반으로 하는 분산 응용 프로그램의 생산성 향상을 위해, 조립식 분산 응용의 작성을 지원하는 분산 객체 조립기, DOC(Distributed Object Composer)를 소개한다. 분산 객체 조립성의 기반이 되는 DOC의 구성적{{) '전체'를 창조해 내기 위해 여러 요소들을 결합, 배치하여 하나의 예술작품을 성립시키는 방법. 모아서 조립한다는 의미.}} 인터페이스(Structural Interface)는 기존 객체지향 기술의 절차적 인터페이스(Procedural Interface)에 비해 콤포넌트 재사용성을 개선하고, 분산 응용의 신속한 개발을 지원하여, 소프트웨어의 생산성 및 확장성을 향상시킨다. 또한, DOC 방식으로 모델링하고 구현한 공급망 관리(Supply Chain Management: SCM) 응용 프로그램의 조립식 작성을 통해, DOC의 실시간 정보 처리 및 조립 기능을 보인다.DOC는 이질적인 통신 환경에서 클라이언트와 서버 객체간의 네트워크 투과성을 제공하는 미들웨어, CORBA를 기반으로 한다. 또한, 분산 객체들 간의 상호작용을 자동 처리하기 위해 Java의 관찰자/피관찰자(Observer/Observable)를 확장한 분산 관찰자/피관찰자 패턴을 정의하여 DOC와, 서버 및 클라이언트의 분산 응용 객체들을 정의하기 위한 인터페이스에 적용한다.Abstract Due to the availability of advances in network and distributed processing technologies, the enhanced productivity of application software that runs on distributed environment is demanded. In this paper, we introduce the Distributed Object Composer (DOC) that supports the structural composition of distributed applications for the enhanced productivity of MVC (Model, View, Controller) pattern-based application programs. The structural interface of DOC that is the basis for the composibility of distributed objects increases the reusability of software components and provides better software productivity and extendibility than the procedural interface of the traditional object-oriented technology by supporting rapid constructions of distributed applications. In this paper, we also discuss the real-time information processing and composibility features of DOC by applying the DOC mechanism to the Supply Chain Management (SCM) application.DOC is implemented using the CORBA middleware that supports the network transparency between client and server objects on heterogeneous communication environments. We apply the distributed Observer/Observable pattern that extends the Observer/Obseverable of Java to DOC itself and distributed client/server objects as their interface definitions for the automatic interactions of distributed objects.

• KIPS Transactions on Computer and Communication Systems
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• v.3 no.11
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• pp.403-408
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• 2014

In the Internet of Things(IoT) era, many of the things or objects that enclose our environments are able to associate with those things on the Internet. To construct IoT systems, it needs to consider a component for acquiring and aggregating of sensory data via things with sensors and instruments, which is connected by diverse networks, in IoT environment. An IoT system is intrinsically distributed in a variety of ways. In addition, to manage an IoT system efficiently, interoperability is needed to meet requirements while the IoT system is designed to deliver data among its applications. In this paper, a reference architecture based on asynchronous messaging is defined and used for designing an IoT system. To apply the architecture, we discuss how to manage data streams with real-time characteristics and make a prototype based on pipe-and-filter to produce and consume them by a pub/sub messaging system NaradaBrokering.

• Journal of KIISE:Computing Practices and Letters
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• v.15 no.8
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• pp.534-546
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• 2009

Query by humming is an effective and intuitive querying mechanism when a user wants to find a song without knowing lyrics. The query by humming system takes a user-hummed melody as input, compares it with melodies in a music database, and returns top-k similar melodies to the input. In this paper, we propose a novel algebra for query by humming, and design and implement a real query by humming system called HummingBase by exploiting the algebra. By analyzing existing similarity search techniques, we derive 10 core operators for the algebra. By using the well-defined algebra, we can easily implement such a system in a extensible and modular way. With two case studies, we show that the proposed algebra can easily represent the query processing processes of existing query-by-humming systems.

• Journal of KIISE:Information Networking
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• v.29 no.2
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• pp.156-164
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• 2002

IPSec is a protocol which provides data encryption, message authentication and data integrity on public and open network transmission. In IPSec, ESP protocol is used when it needs to provide data encryption, authentication and Integrity In real transmission packets. ESP protocol uses DES-CBC encryption mode when sender encrypts packets and receiver decrypts data through this mode IV is used at that time. This value has many tasks of attack during transmission by attacker because it is transferred clean and opened. If IV value is modified, then decryption of ESP data is impossible and higher level information is changed. In this paper we propose a new algorithm that it encrypts IV values using DES-ECB mode for preventing IV attack and checks integrity of whole ESP data using message authentication function. Therefore, we will protect attacks of IV and data, and guarantee core safe transmission on the public network.

• Journal of Microbiology and Biotechnology
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• v.31 no.10
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• pp.1358-1365
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• 2021

To clarify the role of long intergenic nonprotein-coding RNA 1232 (LINC01232) in the progression of gastric cancer and the potential mechanism, we analyzed the expression of LINC01232 in TCGA database using the GEPIA online tool, and the LINC01232 level in gastric cancer cell lines was detected by quantitative real time-polymerase chain reaction (qRT-PCR) as well. Cell proliferation assay, colony formation assay, transwell assay and tumor formation experiment in nude mice were conducted to observe the biological behavior changes of gastric cancer cells through the influence of LINC01232 knockdown. LncATLAS database and subcellular isolation assay were used for subcellular distribution of LINC01232 in gastric cancer cells. The interaction among LINC01232, zeste homolog 2 (EZH2) and kruppel-like factor 2 (KLF2) was clarified by RNA-protein interaction prediction (RPISeq), RNA immunoprecipitation (RIP), qRT-PCR and chromatin immunoprecipitation (ChIP) assay. Rescue experiments were further conducted to elucidate the biological function of LINC01232/KLF2 axis in the progression of gastric cancer. LINC01232 was upregulated in stomach adenocarcinoma (STAD) tissues and gastric cancer lines. LINC01232 knockdown inhibited the proliferative capacities of gastric cancer cells in vitro, and impaired in vivo tumorigenicity. LINC01232 was mainly distributed in the cell nucleus where it epigenetically repressed KLF2 expression via binding to the enhancer of EZH2, which was capable of binding to promoter regions of KLF2 to induce histone H3 lysine 27 trimethylation (H3K27me3). LINC01232 exerts oncogenic activities in gastric cancer via inhibition of KLF2, and therefore, the knockdown of KLF2 could reverse the regulatory effect of LINC01232 in the proliferative ability of gastric cancer cells.

• Journal of Microbiology and Biotechnology
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• v.31 no.10
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• pp.1331-1342
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• 2021

In this study, we evaluated the mechanism of long non-coding RNA MIR22 host gene (LncRNA MIR22HG) in osteosarcoma cells. Forty-eight paired osteosarcoma and adjacent tissues samples were collected and the bioinformatic analyses were performed. Target genes and potential binding sites of MIR22HG, microRNA (miR)-629-5p and tet methylcytosine dioxygenase 3 (TET3) were predicted by Starbase and TargetScan V7.2 and confirmed by dual-luciferase reporter assay. Cell Counting Kit-8, colony formation and flow cytometry assays were utilized to determine the viability, proliferation and apoptosis of transfected osteosarcoma cells. Pearson's analysis was introduced for the correlation analysis between MIR22HG and miR-629-5p in osteosarcoma tissue. Relative expressions of MIR22HG, miR-629-5p and TET3 were measured by quantitative real-time polymerase chain reaction or Western blot. MiR-629-5p could competitively bind with and was negatively correlated with MIR22HG, the latter of which was evidenced by the high expression of miR-629-5p and low expression of MIR22HG in osteosarcoma tissues. Overexpressed MIR22HG repressed the viability and proliferation but enhanced apoptosis of osteosarcoma cells, which was reversed by miR-629-5p upregulation. TET3 was the target gene of miR-629-5p, and the promotive effects of upregulated miR-629-5p on the viability and proliferation as well as its repressive effect on apoptosis were abrogated via overexpressed TET3. To sum up, overexpressed MIR22HG inhibits the viability and proliferation of osteosarcoma cells, which was achieved via regulation of the miR-629-5p/TET3 axis.

• Cross-Cultural Studies
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• v.29
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• pp.55-84
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• 2012

This paper is to study the Tang Dynasty monk poet Ling-yi's poem world and his significance on history of literature. In Chinese literature history, "the monk poets" is a very unique literature creation group. The means of a word "the monk poet", from the literal can see " he is the monk that Can write poems", which is dedicated to professionally writing poetry monk. Buddhism spread to China, from the Wei and Jin Dynasty beginning has been writing poetry of the monks, but the real meaning of "the monk poet" (i.e., professionally poetry monk) appeared to the Mid-Tang Dynasty period. The monk Ling-yi is the pioneer of the monk poets group and Buddist monk creative poem in Mid-tang Dynasty period. Although the Lingyi life is very short, only 35 had died, and his poetry has not been too much, only 44 songs, but he in this life of 35 years and 44 poems, for the development of classical Chinese poetry left noticeable imprinting. He is not just as monk's high practice and by advocating for great Buddhist scholar, also through the daily meditation in poetry creation practice were obtained at that time of many men of literature and writing respected. This paper from the poem monk Ling-yi double identity - the first is a Buddhist monk, the second is addicted to poetry poet to proceed, step by step, in-depth study as the poem monk Ling-yi's poetry creation characteristic and the creative mentality characteristics. This thesis also explores the poem monk by the creation of poetry pursuit, exploration, finally realized "poem" and "Zen" together as one "Zen poetry" creation mechanism.

• Asian-Australasian Journal of Animal Sciences
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• v.32 no.2
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• pp.170-175
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• 2019

Objective: Uncoupling protein 3 gene (UCP3) is a candidate gene associated with the meat quality of pigs. The aim of this study was to explore the regulation mechanism of UCP3 expression and provide a theoretical basis for the research of the function of porcine UCP3 gene in meat quality. Methods: Bisulfite sequencing polymerase chain reaction (PCR) and quantitative real-time PCR (Q-PCR) were used to analyze the methylation of UCP3 5′-flanking region and UCP3 mRNA expression in the adipose tissue or skeletal muscle of three pig breeds at different ages (1, 90, 210-day-old Putian Black pig; 90-day-old Duroc; and 90-day-old Dupu). Results: Results showed that two cytosine-guanine dinucleotide (CpG) islands are present in the promoter region of porcine UCP3 gene. The second CpG island located in the core promoter region contained 9 CpG sites. The methylation level of CpG island 2 was lower in the adipose tissue and skeletal muscle of 90-day-old Putian Black pigs compared with 1-day-old and 210-day-old Putian Black pigs, and the difference also existed in the skeletal muscle among the three 90-day-old pig breeds. Furthermore, the obvious changing difference of UCP3 mRNA expression was observed in the skeletal muscle of different groups. However, the difference of methylation status and expression level of UCP3 gene was not significant in the adipose tissue. Conclusion: Our data indicate that UCP3 mRNA expression level was associated with the methylation status of UCP3 promoter in the skeletal muscle of pigs.

• The Journal of Korean Medicine
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• v.40 no.2
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• pp.35-50
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• 2019

Objectives: Orostachys japonicas (O. japonicus) has been known for its anti-tumor effect. In the present study, it was investigated whether O. japonicus EtOH extracts could induce apoptosis and autophagy which are part of the main mechanism related to anti-tumor effect in THP-1 cells. Methods: Cells were treated with various concentrations of O. japonicus EtOH extracts ($0-300{\mu}g/ml$) for 24, 48, and 72h. Cell viability was evaluated by MTS/PMS assay and apoptosis rate was examined by flow cytometry and ELISA assay. The mRNA expression of apoptosis-related genes (Bcl-2, Mcl-1, Survivin, Bax) and autophagy-related gene (mTOR) was evaluated using real-time PCR. The protein expression of Caspase-3, Akt, LC3 II, Beclin-1, Atg5, $NF-{\kappa}B$, p38, ERK was evaluated using western blot analysis. Results: O. japonicus EtOH extracts inhibited cell proliferation and apoptosis rate was increased in both flow cytometry and ELISA assay. Bcl-2, Mcl-1, Survivin (anti-apoptosis factors) mRNA expressions were decreased and Bax (pro-apoptosis factor) mRNA level was increased. mTOR mRNA expressions was decreased and LC3 II protein expressions was increased. Activation of $NF-{\kappa}B$ was decreased and phosphorylation of p38 was increased. Conclusion: O. japonicus is regarded to inhibit cell proliferation, to induce apoptosis and to regulate autophagy-related genes in THP-1 cells via $NF-{\kappa}B$ and p38 MAPK signaling pathway. This suggests O. japonicus could be an effective herb in treating acute myeloid leukemia.