• Journal of the Korea Institute of Information and Communication Engineering
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• v.14 no.5
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• pp.1303-1310
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• 2010

Recently, researches related with automative mechanism have been widely studied to increase the driver's safety by continuously monitoring the driver's health condition to prevent driver's drowsiness. This paper describes the design of wearable chest belt for ECG and reflectance pulse oximetry for SpO2 sensors based on wireless sensor network to monitor the driver's healthcare status. ECG, SpO2 and heart rate signals can be transmitted via wireless sensor node to base station connected to the server. Intelligent monitoring system is designed at the server to analyze the SpO2 and ECG signals. HRV (Heart Rate Variability) signals can be obtained by processing the ECG and PPG signals. HRV signals are further analyzed based on time and frequency domain to determine the driver's drowsiness status.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.11 no.7
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• pp.2334-2340
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• 2010

Sun position computed by Michalsky shows maximum $1.5^{\circ}$, $0.88^{\circ}$ and 2 minutes differences in azimuth, altitude, and sunrise and sunset times respectively compared with Korean Almanac. The 2-axis solar tracking system, which consist control panel with ATmega128 CPU, BLDC motor-cylinder actuator and 2-axis link mechanism, was developed. Computed azimuth and altitude of sun for a current time, and latitude and longitude of tracker position built are controlled in real time by BLDC motor-cylinder actuators comparing with the position feed-backed by Hall sensor. The use of BLDC motor is free in maintenance. Implementation of a home-return function by Hall sensor is to minimize the cumulative error.

• KIPS Transactions on Software and Data Engineering
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• v.5 no.11
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• pp.521-526
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• 2016

As on-line informal text data have massive in its volume and have unstructured characteristics in nature, there are limitations in applying traditional relational data model technologies for data storage and data analysis jobs. Moreover, using dynamically generating massive social data, social user's real-time reaction analysis tasks is hard to accomplish. In the paper, to capture easily the semantics of massive and informal on-line documents with unsupervised learning mechanism, we design and implement automatic topic extraction systems according to the mass of the words that consists a document. The input data set to the proposed system are generated first, using N-gram algorithm to build multiple words to capture the meaning of the sentences precisely, and Hadoop and Spark (In-memory distributed computing framework) are adopted to run topic model. In the experiment phases, TB level input data are processed for data preprocessing and proposed topic extraction steps are applied. We conclude that the proposed system shows good performance in extracting meaningful topics in time as the intermediate results come from main memories directly instead of an HDD reading.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2009.10a
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• pp.545-548
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• 2009

Recently, researches related to automative mechanism have been widely studied to increase the driver's safety by continuously monitoring the driver's health condition to prevent driver's drowsiness. This paper describes the design of wearable chest belt for ECG and reflectance pulse oximetry for $SpO_2$ sensors based on wireless sensor network to monitor the driver's healthcare status. ECG, $SpO_2$ and heart rate signals can be transmitted via wireless sensor node to base station connected to the server. Intelligent monitoring system is designed at the server to analyze the $SpO_2$ and ECG signals. HRV(Heart Rate Variability) signals can be obtained by processing the ECG and PPG signals. HRV signals are further analyzed based on time and frequency domain to determine the driver's drowsiness status.

• Convergence Security Journal
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• v.17 no.5
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• pp.3-10
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• 2017

Standard Protocol Optimized for Resource-Constrained IoT Environment Constrained Application Protocol (CoAP) supports web-based communication between a sensor node in the IoT environment and a client on the Internet. The CoAP is a Request / Response model that responds to the client's CoAP Request message by responding with a CoAP Response message from the server. CoAP recommends the use of CoAP-DTLS for message protection. However, validation of the use of DTLS in the IoT environment is underway. We analyze CoAP and DTLS security mode, evaluate performance of secure channel creation time, security channel creation step time, and RAM / ROM consumption through Cooja simulator and evaluate the possibility of real environment application.

• Journal of Veterinary Science
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• v.24 no.4
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• pp.53.1-53.12
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• 2023

Background: Mammalian orthoreovirus type 3 (MRV3), which is responsible for gastroenteritis in many mammalian species including pigs, has been isolated from piglets with severe diarrhea. However, the use of pig-derived cells as an infection model for swine-MRV3 has rarely been studied. Objectives: This study aims to establish porcine intestinal organoids (PIOs) and examine their susceptibility as an in vitro model for intestinal MRV3 infection. Methods: PIOs were isolated and established from the jejunum of a miniature pig. Established PIOs were characterized using polymerase chain reaction (PCR) and immunofluorescence assays (IFAs) to confirm the expression of small intestine-specific genes and proteins, such as Lgr5, LYZI, Mucin-2, ChgA, and Villin. The monolayered PIOs and three-dimensional (3D) PIOs, obtained through their distribution to expose the apical surface, were infected with MRV3 for 2 h, washed with Dulbecco's phosphate-buffered saline, and observed. Viral infection was confirmed using PCR and IFA. We performed quantitative real-time reverse transcription-PCR to assess changes in viral copy numbers and gene expressions linked to intestinal epithelial genes and antiviral activity. Results: The established PIOs have molecular characteristics of intestinal organoids. Infected PIOs showed delayed proliferation with disruption of structures. In addition, infection with MRV3 altered the gene expression linked to intestinal epithelial cells and antiviral activity, and these effects were observed in both 2D and 3D models. Furthermore, viral copy numbers in the supernatant of both models increased in a time-dependent manner. Conclusions: We suggest that PIOs can be an in vitro model to study the infection mechanism of MRV3 in detail, facilitating pharmaceutical development.

• Journal of Rhinology
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• v.59 no.6
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• pp.528-537
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• 2021

Background: Urban particulate matter (UPM) in ambient air is implicated in a variety of human health issues worldwide, however, few studies exist on the effect of UPM on the olfactory system. This study aimed to identify the factors affecting the destruction of the olfactory system in a mouse model following UPM exposure. Methods: Mice were divided into: control and four UPM-exposed groups (200 ㎍ UPM at 1 and 2 weeks, and 400 ㎍ UPM at 1 and 2 weeks [standard reference material 1649b; average particle diameter 10.5 ㎛]). The olfactory neuroepithelium was harvested for histologic examination, gene ontology, quantitative real-time polymerase chain reaction, and western blotting. Results: Compared to the control group, olfactory marker protein, Olfr1507, ADCY3, and GNAL mRNA levels were lower, and S-100, CNPase, NGFRAP1, BDNF, and TACR3 mRNA levels were higher in the olfactory neuroepithelium of the UPM groups. Moderately positive correlation was present between the 1- and 2-week groups. After analyzing the 200 and 400 UPM groups separately, the strength of the association between the 200 UPM 1- and 2-week groups was moderately positive. No differences was present in the neuroepithelial inflammatory marker levels between the UPM and control groups. Conclusions: UPM could have cytotoxic effects on the olfactory epithelium. The exposure time and particular concentration of UPM exposure could affect the degree of destruction of the olfactory neuroepithelium. The olfactory regeneration mechanism could be related to the neurotrophic factors, olfactory ensheathing cell stimulation, and trigeminal nerve support.

• BMB Reports
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• v.53 no.9
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• pp.478-483
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• 2020

Kudoa septempunctata is a myxozoan parasite that causes food poisoning in individuals consuming olive flounder. The present study aimed to investigate the currently insufficiently elucidated early molecular mechanisms of inflammatory responses in the intestine owing to parasite ingestion. After Kudoa spores were isolated from olive flounder, HT29 cells were exposed to spores identified to be alive using SYTO-9 and propidium iodide staining or to antigens of Kudoa spores (KsAg). IL-1β, IL-8, TNF-α and NFKB1 expression and NF-κB activation were assessed using real-time PCR, cytokine array and western blotting. The immunofluorescence of FITC-conjugated lectins, results of ligand binding assays using Mincle-Fc and IgG-Fc, CLEC4E expressions in response to KsAg stimulation, and Mincle-dependent NF-κB activation were assessed to clarify the early immune-triggering mechanism. Inflammatory cytokines (IL-1β, GM-CSF and TNF-α), chemokines (IL-8, CCL2, CCL5 and CXCL1) and NF-κB activation (pNF-κB/NF-κB) in HT29 cells increased following stimulation by KsAg. The immunofluorescence results of spores and lectins (concanavalin A and wheat germ agglutinin) suggested the importance of Mincle in molecular recognition between Kudoa spores and intestinal cells. Practically, data for Mincle-Fc and KsAg binding affinity, CLEC4E mRNA expression, Mincle immunofluorescence staining and hMincle-dependent NF-κB activation demonstrated the involvement of Mincle in the early immune-triggering mechanism. The present study newly elucidated that the molecular recognition and immune-triggering mechanism of K. septempunctata are associated with Mincle on human intestinal epithelial cells.

• Journal of Acupuncture Research
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• v.22 no.3
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• pp.23-35
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• 2005

Objective : The aim of the study is to investigate the effect of electro-acupuncture (EA) on ST36 to modulate immune reaction in BALB/c mice immunized intraperitoneally with 2,4-dinitrophenylated keyhole limpet protein(DNP-KLH). Methods : Experimental mice were divided into four groups : 1) Normal group was not performed by any operation. 2) IM(Immunized) group was immunized intraperitoneally with DNP-KLH and aluminum hydroxide without electro-acupunture stimulation. 3) IM-EA(immunized-elctro- acupuncture) group was performed by successive electro-acupuncture on the ST36 acupoint after immunization. 4) IM-NA(immunized-naloxone) group was performed by immunization and electro-acupuncture with same method, but naloxone was injected intraperitoneally 30 minutes before eletro-acupuncture to inhibit the opiate receptor in spleen. Serum total immunoglobulin I(IgE) and antigen-specific IgE was measured in each group. The expression of interferon-${\gamma}$ and interleukin-4 mRNA in spleen was researched by real-time RT-PCR Results : Serum total-IgE and antigen-specific IgE were significantly decreased only in IM-EA group. The expression of interleukin-4 in spleen cell was significantly reduced not only in IM-EA group, but also in IM-EA group. Conclusions : Above results indicate that the mechanism of immunomodulatory effect of electro-acupuncture is related to opioid system especially in B-cell immune reaction. Further research on the T-cell immunity is necessary to explain the mechanism of immunomodulatory effect of electro-acupuncture.

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.23
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• pp.10407-10412
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• 2015

Background: ${\beta}$-elemene, extracted from herb medicine Curcuma wenyujin has potent anti-tumor effects in various cancer cell lines. However, the activity of ${\beta}$-elemene against glioma cells remains unclear. In the present study, we assessed effects of ${\beta}$-elemene on human glioma cells and explored the underlying mechanism. Materials and Methods: Human glioma U87 cells were used. Cell proliferation was determined with MTT assay and colony formation assay to detect the effect of ${\beta}$-elemene at different doses and times. Fluorescence microscopy was used to observe cell apoptosis with Hoechst 33258 staining and change of glioma apoptosis and cell cycling were analyzed by flow cytometry. Real-time quantitative PCR and Western-blotting assay were performed to investigated the influence of ${\beta}$-elemene on expression levels of Fas/FasL, caspase-3, Bcl-2 and Bax. The experiment was divided into two groups: the blank control group and ${\beta}$-elemne treatment group. Results: With increase in the concentration of ${\beta}$-elemene, cytotoxic effects were enhanced in the glioma cell line and the concentration of inhibited cell viability ($IC_{50}$) was $48.5{\mu}g/mL$ for 24h. ${\beta}$-elemene could induce cell cycle arrest in the G0/G1 phase. With Hoechst 33258 staining, apoptotic nuclear morphological changes were observed. Activation of caspase-3,-8 and -9 was increased and the pro-apoptotic factors Fas/FasL and Bax were upregulated, while the anti-apoptotic Bcl-2 was downregulated after treatment with ${\beta}$-elemene at both mRNA and protein levels. Furthermore, proliferation and colony formation by U87 cells were inhibited by ${\beta}$-elemene in a time and does-dependent manner. Conclusions: Our results indicate that ${\beta}$-elemene inhibits growth and induces apoptosis of human glioma cells in vitro. The induction of apoptosis appears to be related with the upregulation of Fas/FasL and Bax, activation of caspase-3,-8 and -9 and downregulation of Bcl-2, which then trigger major apoptotic cascades.