• Molecules and Cells
• /
• v.27 no.3
• /
• pp.383-390
• /
• 2009

To investigate the regulatory mechanism underlying the contractile response in the intestinal smooth muscle of the nile tilapia (Orechromis niloticus), we used pharmacologic and molecular approaches to identify the muscarinic subreceptors and the intracellular signaling pathways involved in this motility. Myography assays revealed that an M1- and M3-subtype selective antagonist, but not a M2-subtype selective antagonist, inhibited carbachol HCl (CCH)-induced intestinal smooth muscle contraction. In addition, a phospholipase C inhibitor, but not an adenylate cyclase inhibitor, blocked the contractile response to CCH. We also cloned five muscarinic genes (OnM2A, OnM2B, OnM3, OnM5A, and OnM5B) from the nile tilapia. In the phylogenetic analysis and sequence comparison to compare our putative gene products (OnMs) with the sequences obtained from the near complete teleost genomes, we unexpectedly found that the teleost fish have respectively two paralogous genes corresponding to each muscarinic subreceptor, and other teleost fish, except zebrafish, do not possess muscarinic subreceptor M1. In addition, the expression pattern of the nile tilapia muscarinic subreceptor transcripts during CCH-induced intestinal smooth muscle contraction in the proximal intestinal tissue was analyzed by real-time PCR surveys and it was demonstrated that CCH increased the OnMs mRNA expression rapidly and transiently.

• Asian-Australasian Journal of Animal Sciences
• /
• v.21 no.3
• /
• pp.325-330
• /
• 2008

In order to understand the molecular mechanism of heterosis of reproduction traits in chickens, we used the quantitative real-time reverse transcriptional polymerase chain reaction (Quantitative real-time RT-PCR) technique to investigate the differential expression of estrogen receptor (ESR) and follicle stimulating hormone receptor (FSHR) genes in 32-week-old ovaries of inbred chickens and their hybrid offspring in $4{\times}4$ diallel crosses, which involved White Plymouth Rock (E), CAU Brown (D), Silkies (C) and White Leghorn (A). We found that there were significant differences in mRNA expression of ESR and FSHR genes not only between hybrids and their parental lines (p<0.01), but also among different crosses (p<0.01). Furthermore, positive correlations between differential expression of both ESR and FHSR in hybrids and heterosis percentages of 32-week-old and 42-week-old egg number traits were significant at p<0.05. Our results suggested that differential expression of ESR and FSHR genes in the ovaries of inbred chickens and their hybrids could play roles in the formation of heterosis of egg number traits to some extent.

• Journal of the Institute of Electronics and Information Engineers
• /
• v.50 no.9
• /
• pp.32-45
• /
• 2013

Globally, intelligent vehicles and telematics research and development through the integration of IT technology in the vehicle are significant increasing. Real-time data communication for intelligent transportation system (ITS) is very important. It collects real-time data from the vehicle and provides the information collected from ITS center. We propose an effective and secure communication scheme for these communication procedures. In particular, our proposed SIP-based MVPN reduces signaling cost and has many advantages in security aspects. In addition, our proposed scheme performs the mobility management applying NEMO (Network Mobility) for the communication between the vehicles. In other words, we propose an ITS communication mechanism of SIP-based mobile VPN and V2V NEMO. Finally, our performance analysis show that the ITS of SIP-based MVPN is significantly reducing the handoff signaling cost.

• Asian Pacific Journal of Cancer Prevention
• /
• v.16 no.10
• /
• pp.4199-4202
• /
• 2015

Background: Hepatitis B virus infection is one of the major world health problems. Epigallocatechin-3 gallate is the major component of the polyphenolic fraction of green tea and it has an anti-viral, anti-mutagenic, anti-tumorigenic, anti-angiogenic, anti-proliferative, and/or pro-apoptotic effects on mammalian cells. In this study, our aim was to investigate the inhibition of HBV replication by epigallocatechin-3 gallate in the Hep3B2.1-7 hepatocellular carcinoma cell line. Materials and Methods: HBV-replicating Hep3B2.1-7 cells were used to investigate the preventive effects of epigallocatechin-3 gallate on HBV DNA replication. The expression levels of HBsAg and HBeAg were determined using ELISA. Quantitative real-time-PCR was applied for the determination of the expression level of HBV DNA. Results: Cytotoxicity of epigallocathechin-3-gallate was not observed in the hepatic carcinoma cell line when the dose was lower than $100{\mu}M$. The ELISA method demonstrated that epigallocatechin-3 gallate have strong effects on HBsAg and HBeAg levels. Also it was detected by real-time PCR that epigallocatechin-3 gallate could prevent HBV DNA replication. Conclusions: The obtained data pointed out that although the exact mechanism of HBV DNA replication and related diseases remains unclear, epigallocatechin-3 gallate has a potential as an effective anti-HBV agent with low toxicity.

• Journal of Korea Multimedia Society
• /
• v.3 no.6
• /
• pp.611-616
• /
• 2000

By the most important media to deliver the contents on a remote instruction we commonly use audio and documents. A number of remote instruction system are trying to offer the video, but they did not acquire satisfiable results because of the limited network and width. Also, they use the general web browsers that have a lot of unspecific users access the contents. Also, they use the general web browsers that have a lot of unspecific users access the contents. Like this most systems that use the continuous media have not offer the satisfiable contents because of the network limitation. Moreover, because they use the web browser, they offer the contents having documents(web pages) only. In this paper, we propose a web-based remote instruction system on real-time using audio and documents which are the most important media for a information delivery. In addition to the system, we use a action synchronization mechanism between the web browsers. If the instructor uses web pages on his computer and explains the contents of them, the learners see the same web pages as the instructor's and listens to his voice.

• Journal of the Korea Institute of Information and Communication Engineering
• /
• v.9 no.4
• /
• pp.844-853
• /
• 2005

Red tide(harmful algae) in the Korean Coastal Waters has a given a given damage to the fishery every year. However, the aim of our study understands the influence of meteorological factors (air and water temperature, precipitation sunshine, solar radiation, winds) relating to the mechanism of red tide occurrence and monitors red tide by satellite remote sensing, and analyzes the potential area for red tide occurrence by GIS. The meteorological factors have directly influenced on red tide formation. Thus, We want to predict and apply to red tide formation from statistical analyses on the relationships between red tide formation and meteorological factors. In future, it should be realized the near real time monitoring for red tide by the development of remote sensing technique and the construction of integrated model by the red tide information management system (the data base of red tide - meteorological informations. Finally our purpose is support to the prediction information for the possible red tide occurrence by coastal meteorological information and contribute to reduce the red tide disaster by the prediction technique for red tide.

• 한국균학회소식:학술대회논문집
• /
• 2015.11a
• /
• pp.35-35
• /
• 2015

Pleurotus eryngii has recently become a major cultivated mushroom; it uses tetrapolar heterothallism as a part of its reproductive process. Sexual development progresses only when the A and B mating types are compatible. Such mating incompatibility occasionally limits the efficiency of breeding programs in which crossing within loci-shared strains or backcrossing strategies are employed. Therefore, understanding the mating system in edible mushroom fungi will help provide a short cut in the development of new strains. We isolated and identified pheromone and receptor genes in the B3 locus of P. eryngii and performed a functional analysis of the genes in the mating process by transformation. A genomic DNA library was constructed to map the entire mating-type locus. The B3 locus was found to contain four pheromone precursor genes and four receptor genes. Remarkably, receptor PESTE3.3.1 has just 34 amino acid residues in its C-terminal cytoplasmic region; therefore, it seems likely to be a receptor-like gene. Real-time quantitative RT-PCR (real-time qRT-PCR) revealed that most pheromone and receptor genes showed significantly higher expression in monokaryotic cells than dikaryotic cells. The pheromone genes PEphb3.1 and PEphb3.3 and the receptor gene PESTE3.3.1 were transformed into P5 (A3B4). The transformants were mated with a tester strain (A4B4), and the progeny showed clamp connections and a normal fruiting body, which indicates the proposed role of these genes in mating and fruiting processes. This result also confirms that PESTE3.3.1 is a receptor gene. In this study, we identified pheromone and receptor genes in the B3 locus of P. eryngii and found that some of those genes appear to play a role in the mating and fruiting processes. These results might help elucidate the mechanism of fruiting differentiation and improve breeding efficiency.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
• /
• 2015.10a
• /
• pp.763-765
• /
• 2015

The study is being conducted to ensure the transparency of research and development have identified the problems of the current system and improve the way out. Such a study about the subject that do not follow either outside the institutional system has a disadvantage compared to an unfulfilled. R & D in order to prevent the misuse and fraud enforcement shall detect abnormal transactions that occur from transactions between research institutions and credit card issuers in real time. In this paper, we propose a detection method for real-time transaction over. It is able to detect and respond fraudulent transactions that may occur in a variety of environments by adding the data obtained by the business rules to derive stopped making detection system.

• Transactions of the Korean Society of Mechanical Engineers
• /
• v.11 no.3
• /
• pp.501-508
• /
• 1987

Experimental studies of particle (TiO$\_$2/) deposition from the laminar hot gas particle flow (about 1565K) onto the cold wall surface (about 1215K-1530K) were carried out by the 'real time' laser light reflectivity method (LLRM) and the photographs of scanning electron microscope(SEM). The LLRM was used for the measurement of thermophoretic deposition rates of small particles (d$\_$p/<3.mu.m), and the photographs of SEM were used for determining what factors control the collection of particles having diameters ranging from 0.2 to 30 microns. Two phenomena are primarily responsible for transport of the particles across the laminar boundary layers and deposition: (1) particle thermophoresis (i.e. particles migration down a temperature gradient), and (2) particle inertial impaction, the former effect being especially larger factor of the particle deposition in its size over the range of 0.2 to 1 microns. And also, this study indicates that thermophoresis can be important for particles as large as 15 microns. Beyond d$\_$p/=16.mu.m, this effect diminishes and the inertial impaction is taken into account as a dominant mechanism of particle deposition. The results of present experiments found to be in close agreement with existing theories.

• Journal of the Korea Institute of Information and Communication Engineering
• /
• v.7 no.8
• /
• pp.1823-1830
• /
• 2003

This paper presents a multiple face detector based on a robust pupil detection technique. The pupil detector uses active illumination that exploits the retro­reflectivity property of eyes to facilitate detection. The detection range of this method is appropriate for interactive desktop and kiosk applications. Once the location of the pupil candidates are computed, the candidates are filtered and grouped into pairs that correspond to faces using heuristic rules. To demonstrate the robustness of the face detection technique, a dual mode face tracker was developed, which is initialized with the most salient detected face. Recursive estimators are used to guarantee the stability of the process and combine the measurements from the multi­face detector and a feature correlation tracker. The estimated position of the face is used to control a pan­tilt servo mechanism in real­time, that moves the camera to keep the tracked face always centered in the image.