• Journal of Microbiology and Biotechnology
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• 제21권1호
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• pp.100-108
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• 2011

A method for the rapid detection and quantification of Newcastle disease virus (NDV) produced in an animal cell culture-based production system was developed to enhance the speed of the NDV vaccine manufacturing process. A SYBR Green I-based real-time RT-PCR was designed with a conventional, inexpensive RT-PCR kit targeting the F gene of the NDV LaSota strain. The method developed in this study was validated for specificity, accuracy, precision, linearity, limit of detection (LOD), limit of quantification (LOQ), and robustness. The validation results satisfied the predetermined acceptance criteria. The validated method was used to quantify virus samples produced in an animal cell culture-based production system. The method was able to quantify the NDV samples from mid- or late-production phases, but not effective on samples from the early-production phase. For comparison with other quantifiable methods, immunoblotting, plaque assay, and tissue culture infectious dose 50 ($TCID_{50}$) assay were also performed with the NDV samples. The results demonstrated that the real-time RT-PCR method is suitable for the rapid quantification of virus particles produced in an animal cell-culture-based production system irrespective of viral infectivity.

• Food Science and Biotechnology
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• 제18권5호
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• pp.1118-1123
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• 2009

Event-specific real-time polymerase chain reaction (PCR) detection method for genetically modified (GM) maize MIR604 was developed based on integration junction sequences between the host plant genome and the integrated transgene. In this study, 2 primer pairs and probes were designed for specific amplification of 100 and 111 bp DNA fragments from the zSSIIb gene (the maize endogenous reference gene) and MIR604. The quantitative method was validated using 3 certified reference materials (CRMs) with levels of 0.1, 1, and 10% MIR604. The method was also assayed with 14 different plants and other GM maize. No amplification signal was observed in real-time PCR assays with any of the species tested other than MIR604 maize. As a result, the bias from the true value and the relative deviation for MIR604 was within the range from 0 to 9%. Precision, expressed as relative standard deviation (RSD), varied from 2.7 to 10% for MIR604. Limits of detections (LODs) of qualitative and quantitative methods were all 0.1%. These results indicated that the event-specific quantitative PCR detection system for MIR604 is accurate and useful.

• International Journal of Advanced Culture Technology
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• 제5권1호
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• pp.51-57
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• 2017

Digital signage is becoming part of daily life across a wide range of visual advertisements segments market used in stations, hotels, retail stores, hotels, etc. The current digital signage system used in market is generally works on limited user interactivity with static contents. In this paper, a new approach is proposed using computer vision based dynamic audience adaptive cost-effective digital signage system. The proposed design uses the Camera attached Raspberry Pi Open source platform to employ the real-time audience interaction using computer vision algorithms to extract facial features of the audience. The real-time facial features are extracted using Haar Cascade algorithm which are used for audience gender specific rendering of dynamic digital signage content. The audience facial characterization using Haar Cascade is evaluated on the FERET database with 95% accuracy for gender classification. The proposed system, developed and evaluated with male and female audiences in real-life environments camera embedded raspberry pi with good level of accuracy.

• 국제학술발표논문집
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• The 5th International Conference on Construction Engineering and Project Management
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• pp.386-389
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• 2013

Under climate change and urbanization, rainwater harvesting (RWH) systems are emerging as an alternative source of water supply because of growing concern about water sustainability. RWH systems can satisfy the various watering needs and provide the environmental benefits of lessening the damages from flood, drought, and runoff. The economic success of a RWH system is vitally concerned with the determination of the design capacity of storage tank to be built in the system. The design capacity is determined by the factors of average annual rainfall, period of water scarcity, and water price during the whole life-cycles. Despite the high uncertainties inherent in these factors, the current engineering design of RWH system construction often assumes that storage tanks should be built all at once. This assumption implicitly ignores the managerial flexibility in responds to the future as new information comes out-the right to build storage tanks stage by stage depending on the evolution of demand. This study evaluates the value of a multistage storage tank construction using a real option approach. A case study involving a typical RWH system construction in Jeonju, the Republic of Korea is conducted. The managerial flexibility obtained from the real option perspective allows engineers to develop investment strategies to better cope with the issue of water sustainability.

• Mycobiology
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• 제49권1호
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• pp.86-88
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• 2021

The monokaryotic strain, Schizophyllum commune strain IUM1114-SS01, was generated from a basidiospore of dikaryotic parental strain IUM1114. It even showed the decolorizing activities for several textile dyes much better than its parental strain. Based on the results of a single-molecule real-time sequencing technology, we present the draft genome of S. commune IUM1114-SS01, comprising 41.1 Mb with GC contents of the genome were 57.44%. Among 13,380 protein-coding genes, 534 genes are carbon hydrate-active enzyme coding genes.

• 한국수자원학회:학술대회논문집
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• 한국수자원학회 2005년도 학술발표회(1)
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• pp.446-447
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• 2005

• 한국농림기상학회:학술대회논문집
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• 한국농림기상학회 2013년도 추계 학술발표논문집
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• pp.79-85
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• 2013

• 성인간호학회지
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• 제14권3호
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• pp.438-448
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• 2002

Purpose: This study is aimed to search the lived experiences of the participants and to analyze the contents so that we can be assured about what the elderly wish and find the appropriate nursing intervention for them in real life. Method: This is based on a phenomenological approach. Participants of this study consist of people older than 65 years old. Data was collected from May to August in 2001. Data collection was done through in-depth interviews and observations. The time used in each interview was from 50 minutes to 2 hours. Each participant was interviewed three to five times. Giorgi's phenomenological analysis was used in data analysis. Result: The elder's wishes are 1) a respected life, 2) a happy life, 3) a peaceful life, 4) a independent life, 5) a financially independent life, 6) a satisfactory life, 7) a mind to depend on someone, 8) a serving life, 9) a historic family, 10) a happy-ending life. Conclusion: The study will contribute for the nursing intervention to enhance the quality of life and successful aging of the elderly.

• 한국윤활학회:학술대회논문집
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• 한국윤활학회 2001년도 제34회 추계학술대회 개최
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• pp.172-178
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• 2001

Using dislocation pileup theory, the near surface crack initiation life was calculated. The crack initiation life calculted in the previous study is not a real life strictly and just for the cracking in substrate. In this study, two life equations which can be applied for each near-surface and substrate were used for a comparative study. The downward tendency of life at near surface and substrate was similar and the crack initiation life at near-surface was much shorter than the life in substrate. The improvement of the crack initiation life equations which were proposed by W. Cheng was discussed.

• 한국콘텐츠학회논문지
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• 제22권5호
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• pp.203-213
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• 2022

본 연구는 삶의 질 저하가 공정성 인식에 미치는 영향 및 부동산 규제 완화에 대한 지지에 미치는 영향에 대해 살펴보고자 하였다. 삶의 질 저하로 인한 불만감의 고조는 외부 사회 시스템의 공정성에 대한 귀책을 가중시키고, 이러한 공정성 측면의 평가절하는 그러한 불공정성을 바로 잡기 위한 정부의 인위적 개입, 즉 정부 주도의 시장 규제 강화에 대한 지지를 증진시킬 수 있다. 또한, 삶의 질 저하로 인한 공정성 차원의 평가절하는 개인의 계층상승에 대한 전망이 비관적일 때 보다 더 증폭될 수 있다. 불공정한 사회 시스템의 운영으로 인한 개인적 타격이 보다 크게 예상될 때, 즉 계층상승에 대한 전망이 비관적일 때 사적 차원의 삶의 질 저하문제는 공적 차원의 공정성 문제로까지 이어질 가능성이 보다 증가될 수 있다. 본 연구는 총 6300명을 대상으로 한 설문자료를 활용해 이러한 예측들에 대한 검증을 시도했으며, 분석 결과는 이를 지지하고 있다. 이러한 결과는 공정성 문제가 최근 쟁점 사안으로 부상하게 된 데 대한 기저 원인을 구체적으로 조망해 볼 기회를 제공하여 주며 나아가 정부의 부동산 규제 완화 혹은 강화에 대한 지지가 공정성 및 계층상승에 대한 전망에 어떻게 영향받게 되는지에 대한 이해를 넓혀주는데 기여하여 줄 수 있을 것이다.