• Proceedings of the Korean Society of Tribologists and Lubrication Engineers Conference
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• 1998.10a
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• pp.91-99
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• 1998

A correlation between the low-cycle fatigue life and the scuffing-failure life is demonstrated using the plastic strain in boundary lubricated sliding. Loadings proportional to hardness with three different lubricated conditions were used to evaluate the plastic strain. As the results of scuffing tests using vacuum pump oils in nitrogen gas, plastic strain shows 0.0062, and In the mineral oils and commercial engine oils in air, plastic strain show 0.0042 and 0.00092. Those are very useful to describe quantitatively the real lubricated sliding conditions, and are very effective to find the relation between the low-cycle fatigue life and the scuffing life.

• Proceedings of the Korean Society for Technology of Plasticity Conference
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• 1998.03a
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• pp.94-99
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• 1998

In bulk metal forming processes prediction of tool life is very important for saving production cost and achieving good material properties. Generally the service life of tools in metal forming process is limited to a large extent by wear, fracture and plastic deformation of tools. In case of hot and warm forging processes, tool life depends on wear over 70%. In this study finite element analyses are applied to warm forging and hot forging by adopting suggested wear model. By comparision of simulation and real profile of die, suggested model is verified

• Proceedings of the Korean Society for Technology of Plasticity Conference
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• 1998.06b
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• pp.54-59
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• 1998

In this study, two kinds of life prediction method for hot forming die are developed . One is empirical method requiring some experiment that evaluate thermal softening of die material accoring to operating conditions. The other is analyticl method that calcuate wear quantity of die occuring during the forming process. Wear is a predominant factor as well as plastic deformation and heat checking . And, these methods are applied to prodict tool life real die producting part for automobile. Thus , the applicability and the accuracy of the presented methods are investigated. Using the verified life prediction method above , optimal blocker die design minimizing the finisher die is done.

• IE interfaces
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• v.6 no.2
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• pp.37-51
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• 1993

This paper studies the economic replacement method for production facilities which requires huge investment but are necessary for higher productivity and competability of products. That is, the general models for the decision of economic life of properties which minimizes the total costs throughout the usage life(Life cycle costs) are generated. Main factors which make influences for the decision of econmic life can be divided by three. These are the change of salvage value, repair and maintenance costs, and availability of production facilities with the passage of usage time. In this paper, the real world data for these three factors are collected and analyzed for the extraction of representative standard forms with the passage of time. And general models for economic replacement methods and optimal usage terms are presented through tables with the combination of the standard forms of these three main factors.

• Asian-Australasian Journal of Animal Sciences
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• v.30 no.3
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• pp.328-337
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• 2017

Objective: An experiment was conducted to determine the relationship between the KAP11.1 and the regulation wool fineness. Methods: In previous work, we constructed a skin cDNA library and isolated a full-length cDNA clone termed KAP11.1. On this basis, we conducted a series of bioinformatics analysis. Tissue distribution of KAP11.1 mRNA was performed using semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) analysis. The expression of KAP11.1 mRNA in primary and secondary hair follicles was performed using real-time PCR (real-time polymerase chain reaction) analysis. The expression location of KAP11.1 mRNA in primary and secondary hair follicles was performed using in situ hybridization. Results: Bioinformatics analysis showed that KAP11.1 gene encodes a putative 158 amino acid protein that exhibited a high content of cysteine, serine, threonine, and valine and has a pubertal mammary gland) structural domain. Secondary structure prediction revealed a high proportion of random coils (76.73%). Semi-quantitative RT-PCR showed that KAP11.1 gene was expressed in heart, skin, and liver, but not expressed in spleen, lung and kidney. Real time PCR results showed that the expression of KAP11.1 has a higher expression in catagen than in anagen in the primary hair follicles. However, in the secondary hair follicles, KAP11.1 has a significantly higher expression in anagen than in catagen. Moreover, KAP11.1 gene has a strong expression in inner root sheath, hair matrix, and a lower expression in hair bulb. Conclusion: We conclude that KAP11.1 gene may play an important role in regulating the fiber diameter.

• Journal of Korea Entertainment Industry Association
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• v.13 no.1
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• pp.121-138
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• 2019

A term 'Wolabal' which is an abbreviation of the words, in South Korea means the balance between work and life. The term reflects on people's thoughts to seek for their happiness these days. In fact, they think that the quality of the life is more valuable than any other things on their lives, reflecting on the issues caused by modern societies. 'Wolabal' has emerged as an alternatives to solve the social issues like economic recession, high unemployment rate, aging society, low birth rate and etc. However, in order to establish 'Wolabal' as a culture the comprehensive agreement between an individual and society should be considered first. In society, the system or policies to forster cultural business should be settled while in individual, it is necessary to change the way they think about their work and the qualities of their lives. From this view we have to focus on the relationship between their work and leisure. On the relationship between their work and leisure we should understand that the relationship is not conflicted but co-exists and understanding the real meaning of the relationship is critical in balancing between work and life. The recognition to the labor which has been from the past would give not only the meaning of individual survival but the one of their whole lives. Despite this, modern society has faded away the real meaning of labor because it has focused on the mass manufacturing and sometimes the long-termed economic sluggish has emerged. This trend has made people think about their lives and seek for their lives' real value.

• Journal of Marine Life Science
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• v.1 no.2
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• pp.79-87
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• 2016

Heavy metals such as cadmium (Cd) are highly toxic to aquatic organisms and human, even at trace concentration. Herein we investigated the effect of Cd on the gene expression of ATP-binding cassette (ABC) transporters and glutathione S-transferase (GST) in marine ciliate Euplotes crassus. Seven ABC transporters and one GST genes were partially cloned and sequences, and thereafter, transcriptional modulation of these genes after exposure to Cd for 8 h was investigated using quantitative real time RT- PCR (qRT-PCR). As results, sequence analysis and phylogenetic study revealed that E. crassus ABCs are likely typical ABC transports, in particular, B/C family, and GST gene may be similar to GST theta isoform. A significant increase in the expression of ABCs, except for ABCB21 was observed in a concentration dependent manner after exposure to Cd (0.1 and 0.5 mg/l) for 8 h. The GST mRNA level was the highest at 0.5 mg/l Cd and then reduced until control level. These findings suggest that ABCs and GST may be involved in a protective mechanism against Cd-mediated toxicity in E. crassus.

• Asian-Australasian Journal of Animal Sciences
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• v.31 no.10
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• pp.1550-1557
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• 2018

Objective: Circular RNAs (circRNAs) are a newfound class of non-coding RNA in animals and plants. Recent studies have revealed that circRNAs play important roles in cell proliferation, differentiation, autophagy and apoptosis during development. However, there are few reports about muscle development-related circRNAs in livestock. Methods: RNA sequencing analysis was employed to identify and annotate circRNAs from longissimus dorsi of sheep. Reverse transcription followed by real-time quantitative (q) polymerase chain reaction (PCR) analysis verified the presence of these circRNAs. Targetscan7.0 and miRanda were used to analyse the interaction of circRNA-microRNA (miRNA). To investigate the function of circRNAs, an experiment was conducted to perform enrichment analysis hosting genes of circRNAs using gene ontology (GO) and Kyoto encyclopedia of genes and genomes (KEGG) pathways. Results: About 75.5 million sequences were obtained from RNA libraries of sheep skeletal muscle. These sequences were mapped to 729 genes in the sheep reference genome. We identified 886 circRNAs, including numerous circular intronic RNAs and exonic circRNAs. Reverse transcription PCR (RT-PCR) and DNA sequencing analysis confirmed the presence of several circRNAs. Real-Time RT-PCR analysis exhibited resistance of sheep circRNAs to RNase R digestion. We found that many circRNAs interacted with muscle-specific miRNAs involved in growth and development of muscle, especially circ776. The GO and KEGG enrichment analysis showed that hosting genes of circRNAs was involved in muscle cell development and signaling pathway. Conclusion: The study provides comprehensive expression profiles of circRNAs in sheep skeletal muscle. Our study offers a large number of circRNAs to facilitate a better understanding of their roles in muscle growth. Meanwhile, we suggested that circ776 could be analyzed in future study.

• Journal of the Computational Structural Engineering Institute of Korea
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• v.26 no.3
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• pp.199-205
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• 2013

In this study, the methodology for the fatigue life prediction using finite element method(FEM) in wire, bundle and assembly level of the wire harness system and the development of the fatigue life test machine for the numerical analysis are investigated. To obtain stress-life(S-N) histories of the componential wires of the system, five kinds of wires are prepared and applied to the repeated bending motion using developed fatigue life test equipment. Equivalent model of the wire from the rule of mixtures theory is used for the material modeling of sheath and wire core combination. Contact conditions among the wires, taping conditions are established through the bundle level test and numerical bundle analysis. Wire and bundle level results are adopted for the assembly level analysis. For the assembly level analysis, real wire harness system including bundle and grommet is numerically modeled and applied contact condition between wires with real opening motion. The fatigue life more than 700,000 cycles of the assembly is obtained from the FEM, and it is confirmed that the result has good agreement with the experimental result.

• Animal cells and systems
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• v.9 no.3
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• pp.153-160
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• 2005

Most living organisms exhibit the circadian rhythm in their physiology and behavior. Recent identification of several clock genes in mammals has led to the molecular understanding of how these components generate and maintain the circadian rhythm. Many reports have implicated the photic induction of either mPer1 or mPer2 in the hypothalamic region called the suprachiasmatic nucleus (SCN) to phase shift the brain clock. It is now established that peripheral tissues other than the brain also express these clock genes and that the clock machinery in these tissues work in a similar way to the SCN clock. To determine the role of the two canonical clock genes, mPer1 and mPer2, in the peripheral clock shift, stable HEK293EcR cell lines that can be induced and stably express these proteins were prepared. By regulating the expression of these proteins, it could be shown that induction of the clock genes, either mPer1 or mPer2 alone is not sufficient to cause clock phase shifting in these cells. Our real-time PCR analysis on these cells indicates that the induction of mPER proteins dampens the expression of the clock-specific transcription factor mBmal1. Altogether, our present data suggest that mPer1 and mPer2 may not function in clock shift or take part in differential roles on the peripheral circadian clock.