• Korean Journal of Fisheries and Aquatic Sciences
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• v.35 no.5
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• pp.490-496
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• 2002

In order to detect the DNA heteropolymorphism of chum salmon, selected essential genes were examined in different regional chum salmons, i.e., Korean, Japanese and American by denaturing gradient gel electrophoresis (DGGE) and real time PCR methods. From the promoter regions and introns of growth hormone, mtDNA NDI region, D-loop region, IGF-I, histone H3 and MCH2 several representative primer pairs were obtained and employed for the DGGE with the PCR products from the genomic DNAs of the different regional chum salmons. mtDNA NDI, D-loop region and IGE-I genes showed marked heteropolymorphism between Korean and American chum salmons. Intron C of growth hormone also showed a heteropolymorphism between Korean and Japanese chum salmons. Whereas heteropolnnorphism of histone liH and MCH2 genes was detected among in Korean, Japanese and Asnerican chum salmons in the examined region. The real time PCR disclosed the characteristic incremental production of target DNAs dependent on the heteropolymorphic conditions of genomic DNAa of chum salmons, thus the different regional chum salmons could be grouped by the variable incremental curies. Although the DGGE and real time PCR did not produce the identical results in this study, we suggest that the DGGE and real time PCR could be used for the primary screening of the DNA heteropolymorphism of different animal genome.

• Annals of Clinical Microbiology
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• v.21 no.4
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• pp.86-91
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• 2018

Background: The PANA RealTyper HPV kit (PANAGENE, Korea; PANA RealTyper) was developed to genotype human papillomavirus (HPV) and was based on multiplex real-time PCR amplification and melting curve analysis. In this study, we compared PANA RealTyper to the AdvanSure HPV GenoBlot assay (LG Life Sciences, Korea; AdvanSure assay) and attempted to evaluate the performance of PANA RealTyper. Methods: A total of 60 cervical specimens were collected from women undergoing routine cervical cancer screening. The AdvanSure assay and PANA RealTyper kit identified the same 20 high-risk genotypes. However, the AdvanSure assay identified 15 low-risk genotypes, while the PANA RealTyper kit identified only 2 but detected 18 low-risk genotypes. Results: Among the total 60 specimens, 54 high-risk genotypes (40 specimens) and 20 low-risk genotypes (18 specimens) were detected. The agreement rates of the assays ranged from 94.4 to 100% for high-risk genotypes. Among 9 genotypes that were positive in the PANA RealTyper kit but negative in the AdvanSure assay, 7 were confirmed as true positive (HPV genotypes 16 (n=1), 39 (n=1), 52 (n=1), 58 (n=2), 68 (n=2)). Among 4 genotypes that were negative in the PANA RealTyper kit but positive in the AdvanSure assay, 3 were confirmed as HPV genotype 59. Among the 19 low-risk genotypes positive in the AdvanSure assay, there were 2 cases of HPV 6 and 1 case of HPV 11. In comparison, only 1 positive case of HPV 6 was determined by the PANA RealTyper kit. Conclusion: The PANA RealTyper kit was comparable with the AdvanSure assay. The PANA RealTyper kit would be useful and suitable for HPV genotyping in the clinical laboratory.

• 제어로봇시스템학회:학술대회논문집
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• 1988.10a
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• pp.338-342
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• 1988

Recently, in sequence control systems, high flexibility and maintenance of control software are required. This is because product life cycles become shorter and control specification must be changed frequently. The authors extend the concept of Safe Petri Net to develop the design and analysis tool for sequence control systems taking the safeness and notation of input/output functions into consideration. Extended Safe Petri Net (S-Net) is proposed as such a new graph model and real time scanning algorithm based on S-Net is developed.

• 제어로봇시스템학회:학술대회논문집
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• 2003.10a
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• pp.2151-2154
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• 2003

Internet is deeply rooted in everyday life and many things are performed using internet in real-world, therefore internet users increased because of convenience. Also internet accident is on the increase rapidly. The security vendor developed security system to protect network and system from intruder. Many hackings can be prevented and detected by using these security solutions. However, the new hacking methods and tools that can detour or defeat these solutions have been emerging and even script kids using these methods and tools can easily hack the systems. In consequence, system has gone through various difficulties. So, Necessity of intruder trace-back technology is increased gradually. Trace-back technology is tracing back a malicious hacker to his real location. trace-back technology is largely divided into TCP connection trace-back and IP packet trace-back to trace spoofed IP of form denial-of-service attacks. TCP connection trace-back technology that autonomously traces back the real location of hacker who attacks system using stepping stone at real time. In this paper, We will describe watermark trace-back system using TCP hijacking technique to supplement difficult problem of connection maintenance happened at watermark insertion. Through proposed result, we may search attacker's real location which attempt attack through multiple connection by real time.

• Journal for History of Mathematics
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• v.21 no.2
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• pp.135-152
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• 2008

In this paper, we survey the thought of students for the reason of the study of mathematics, for mathematics, for the textbook of mathematics and the attitude appling mathematical knowledge in the real life and analyze that. We have a correct understanding how to study mathematics and that motivates study of mathematics to students. Student have a correct understanding how to use basic knowledge of mathematical theory in the real life and have for the study of mathematics. In this article, we investigate the reason for studying mathematics in the real life and analyze the way how to use basic knowledge of mathematical theories through actual examples. The reasons for studying math are divided into 3 categories: mathematics for obtaining common sense and wisdom, practical mathematics for application, and mathematics as a liberal art for promoting our characters and recreation. We investigate the reasons for studying mathematics in each category. By theses results, we make the effectual educational method for mathematics and investigate the effect.

• ALGAE
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• v.31 no.2
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• pp.167-174
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• 2016

Biological response of cells to variable conditions should affect the expression level of certain genes. Quantification of these changes in target genes needs stable internal controls. Real-time quantitative polymerase chain reaction (PCR) has traditionally used reference or ‘housekeeping’ genes, that are considered to maintain equal expression in different conditions, to evaluate changes in target genes between samples and experimental conditions. Recent studies showed that some housekeeping genes may vary considerably in certain biological samples. This has not been evaluated in red algae. In order to identify the optimal internal controls for real-time PCR, we studied the expression of eleven commonly used housekeeping genes; elongation factor 1-alpha, glyceraldehyde-3-phosphate dehydrogenase, β-actin, polyubiquitin, 30S ribosomal gene, 60S ribosomal gene, beta-tubulin, alpha-tubulin, translation initiation factor, ubiquitin-conjugating enzyme, and isocitrate dehydrogenase in different life-history stages of Bostrychia moritziana. Our results suggest that glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and 30S ribosomal gene, have the most stable gene expression levels between the different life history stages (male, female, carposporophyte, and tetrasporophyte), while the other genes are not satisfactory as internal controls. These results suggest that the combinations of GAPDH and 30S would be useful as internal controls to assess expression level changes in genes that may control different physiological processes in this organism or that may change in different life history stages. These results may also be useful in other red algal systems.

• Journal of the Korean School Mathematics Society
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• v.5 no.2
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• pp.71-82
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• 2002

This study purported to develop the learning materials for the optional activities of mathematics, apply them to the students, and examine the effect on the learner's interest and attitude to mathematics. In order to conduct this study, the learning materials for the mathematics optional activities were developed according to the units of grade 8-Ga and the developed learning materials of the mathematics optional activities were inputted in two classes for 18 units after the second grade middle school students in Daejeon area received the questionnaire regarding the learning attitude and the interest in the mathematics. After that, the questionnaire regarding the learning attitude and the interest in the mathematics and the examination regarding the response to the application of the learning materials for mathematics optional activities were conducted and did t-test. The results of the study as follows : First The learning materials applied exciting games for mathematics optional activities aroused interest in mathematics and made the learners have confidence in mathematics learning. Second, There was affirmative change in the interest and attitude to the mathematics learning with the application of the mathematics optional learning materials. Third The leaning materials of the mathematics optional activities made the learners think mathematical questions connected with the real life and try various ways to solve the questions. Here are some suggestions to study the development of the learning materials such as story, game, quiz and craft. First it is necessary to develop the learning materials applied the games or the real life matters connected with mathematics for all grade middle school students and use them in the mathematics optional activities. Second, many students feel a great interest in animation. moving picture, sound graphic, etc. If the multimedia learning materials included the real life matters or the games are developed, the learners will feel more interest in mathematics. Third, the systematic and economical support is continuously needed in order that teachers are given much time to study and the opportunity to attend the training course for the understanding of the optional activities and the development of the various learning materials.

• Journal of Biosystems Engineering
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• v.36 no.1
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• pp.48-57
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• 2011

The computer vision system was designed and constructed to measure the weight of a dairy cattle. Its development involved the functions of image capture, image preprocessing, image algorithm, and control integrated into one program. The experiments were conducted with the model dairy cattle and the real dairy cattle by two ways. First experiment with the model dairy cattle was conducted by using the indoor vision experimental system, which was built to measure the model dairy cattle in the laboratory. Second experiment with real dairy cattle was conducted by using the outdoor vision experimental system, which was built for measuring 229 heads of cows in the cattle facilities. This vision system proved to a reliable system by conducting their performance test with 15 heads of real cow in the cattle facilities. Indirect weight measuring with four methods were conducted by using the image processing system, which was the same system for measuring of body parameters. Error value of transform equation using chest girth was 30%. This error was seen as the cause of accumulated error by manually measurement. So it was not appropriate to estimate cow weight by using the transform equation, which was calculated from pixel values of the chest girth. Measurement of cow weight by multiple regression equation from top and side view images has relatively less error value, 5%. When cow weight was measured indirectly by image surface area from the pixel of top and side view images, maximum error value was 11.7%. When measured cow weight by image volume, maximum error weight was 57 kg. Generally, weight error was within 30 kg but maximum error 10.7%. Volume transform method, out of 4 measuring weight methods, was minimum error weight 21.8 kg.

• School Mathematics
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• v.11 no.3
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• pp.513-526
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• 2009

The purpose of this paper intuitively shows the exact and logical explanation of Independent Event and Dependent Event. In actual classrooms, teachers have difficulty in describing the connection between those events and real life. Some teachers have wrong perceptions on the definition of those events. For example, they may not realize exactly what P(B A)=P(B) means and may not explain intuitively the original meaning of why it is independent event. Also they believe that Independent Event and Dependent Event do not always match with real life. This paper, therefore, tries to prove intuitively the exact meanings of those events in the Verbal Form with some examples and it proves that those events exactly match with real life. It is expected that this paper will greatly contribute to the improvement of probability education.

• The Mathematical Education
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• v.55 no.3
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• pp.317-334
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• 2016

Knowledge and data interpretation on statistical estimation was important to have statistical literacy that current curriculum was said not to satisfy. The author investigated mathematics teachers' MKT on statistical estimation concerning interpretation of confidence interval by using questionnaire and interview. SMK of teachers' confidence was limited to the area of textbooks to be difficult to interpret data of real life context. Most of teachers wrongly understood SMK of interpretation of confidence interval to have influence upon PCK making correction of students' wrong concept. SMK of samples and sampling distribution that were basic concept of reliability and confidence interval cognized representation of samples rather exactly not to understand importance and value of not only variability but also size of the sample exactly, and not to cognize appropriateness and needs of each stage from sampling to confidence interval estimation to have great difficulty at proper teaching of statistical estimation. PCK that had teaching method had problem of a lot of misconception. MKT of sample and sampling distribution that interpreted confidence interval had almost no relation with teachers' experience to require opportunity for development of teacher professionalism. Therefore, teachers were asked to estimate statistic and to get confidence interval and to understand concept of the sample and think much of not only relationship of each concept but also validity of estimated values, and to have knowledge enough to interpret data of real life contexts, and to think and discuss students' concepts. So, textbooks should introduce actual concepts at real life context to make use of exact orthography and to let teachers be reeducated for development of professionalism.